UniProtKB - Q8CCN5 (BCAS3_MOUSE)
BCAS3 microtubule associated cell migration factor
Bcas3
Functioni
Functions synergistically with PELP1 as a transcriptional coactivator of estrogen receptor-responsive genes. Stimulates histone acetyltransferase activity. Binds to chromatin (By similarity).
Plays a role in angiogenesis. Participates in the regulation of cell polarity and directional endothelial cell migration by mediating both the activation and recruitment of CDC42 and the reorganization of the actin cytoskeleton at the cell leading edge. Promotes filipodia formation. Plays a regulatory role in autophagic activity. In complex with PHAF1, associates with the preautophagosomal structure during both non-selective and selective autophagy. Probably binds phosphatidylinositol 3-phosphate (PtdIns3P) which would mediate the recruitment preautophagosomal structures (By similarity).
By similarity1 PublicationMiscellaneous
GO - Molecular functioni
- acetyltransferase activator activity Source: MGI
- beta-tubulin binding Source: UniProtKB
- chromatin binding Source: UniProtKB
- histone acetyltransferase binding Source: MGI
- histone binding Source: MGI
- nuclear receptor binding Source: MGI
- phosphatidylinositol binding Source: UniProtKB
GO - Biological processi
- activation of GTPase activity Source: UniProtKB
- angiogenesis Source: UniProtKB-KW
- autophagy Source: InterPro
- cellular response to estrogen stimulus Source: UniProtKB
- Golgi organization Source: UniProtKB
- microtubule organizing center organization Source: UniProtKB
- negative regulation of focal adhesion assembly Source: UniProtKB
- negative regulation of GTPase activity Source: UniProtKB
- positive regulation of actin cytoskeleton reorganization Source: UniProtKB
- positive regulation of catalytic activity Source: UniProtKB
- positive regulation of endothelial cell migration Source: UniProtKB
- positive regulation of filopodium assembly Source: UniProtKB
- positive regulation of GTPase activity Source: UniProtKB
- positive regulation of intracellular protein transport Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of establishment of cell polarity Source: UniProtKB
- response to starvation Source: GO_Central
- tube formation Source: UniProtKB
Keywordsi
Biological process | Angiogenesis, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: BCAS3 microtubule associated cell migration factorAlternative name(s): Breast carcinoma-amplified sequence 3 homologBy similarity K20D41 Publication Protein rudhira1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2385848, Bcas3 |
VEuPathDBi | HostDB:ENSMUSG00000059439 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Nucleus
- Nucleus By similarity
Other locations
- Preautophagosomal structure By similarity
Note: Associates with chromatin. Recruited to estrogen receptor-induced promoters in a PELP1-dependent manner (By similarity). Localizes in the cytoplasm in stationary cells. Translocates from the cytoplasm to the leading edge in motile cells. Colocalizes with microtubules and intermediate filaments in both stationary and motile cells. The BCAS3:PHAF1 complex is recruited to the preautophagosomal structures adjacent to the damaged mitochondria upon mitophagy in a PRKN-PINK1 dependent manner (By similarity).By similarity1 Publication
Cytoskeleton
- cytoplasmic microtubule Source: UniProtKB
- intermediate filament cytoskeleton Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Other locations
- cell leading edge Source: UniProtKB
- cell periphery Source: MGI
- cytoplasm Source: UniProtKB
- phagophore assembly site Source: UniProtKB
- transcriptionally active chromatin Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050884 | 1 – 928 | BCAS3 microtubule associated cell migration factorAdd BLAST | 928 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Cross-linki | 215 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 215 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 461 | PhosphoserineCombined sources | 1 | |
Modified residuei | 480 | PhosphoserineCombined sources | 1 | |
Modified residuei | 488 | PhosphoserineCombined sources | 1 | |
Modified residuei | 838 | PhosphoserineCombined sources | 1 | |
Modified residuei | 886 | PhosphoserineCombined sources | 1 | |
Modified residuei | 898 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8CCN5 |
jPOSTi | Q8CCN5 |
MaxQBi | Q8CCN5 |
PaxDbi | Q8CCN5 |
PeptideAtlasi | Q8CCN5 |
PRIDEi | Q8CCN5 |
ProteomicsDBi | 273734 [Q8CCN5-1] 273735 [Q8CCN5-2] |
PTM databases
iPTMneti | Q8CCN5 |
PhosphoSitePlusi | Q8CCN5 |
SwissPalmi | Q8CCN5 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000059439, Expressed in brain and 275 other tissues |
ExpressionAtlasi | Q8CCN5, baseline and differential |
Genevisiblei | Q8CCN5, MM |
Interactioni
Subunit structurei
Interacts with histone H3, ESR1, KAT2B and PELP1; the interactions occur in a estrogen-dependent manner.
Interacts with beta-tubulin and VIM.
Interacts (via C-terminal) with PHAF1; the interaction is requrired for the association with the phagophore (By similarity).
By similarityGO - Molecular functioni
- beta-tubulin binding Source: UniProtKB
- histone acetyltransferase binding Source: MGI
- histone binding Source: MGI
- nuclear receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 228674, 2 interactors |
STRINGi | 10090.ENSMUSP00000103696 |
Miscellaneous databases
RNActi | Q8CCN5, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 254 – 312 | Required for recruitment to preautophagosomal structure in response to mitophagyBy similarityAdd BLAST | 59 | |
Regioni | 437 – 560 | Required for recruitment to preautophagosomal structure in response to mitophagyBy similarityAdd BLAST | 124 | |
Regioni | 472 – 518 | DisorderedSequence analysisAdd BLAST | 47 | |
Regioni | 795 – 816 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 868 – 928 | DisorderedSequence analysisAdd BLAST | 61 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 473 – 517 | Polar residuesSequence analysisAdd BLAST | 45 | |
Compositional biasi | 799 – 816 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 882 – 928 | Polar residuesSequence analysisAdd BLAST | 47 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2109, Eukaryota KOG4415, Eukaryota |
GeneTreei | ENSGT00390000006454 |
HOGENOMi | CLU_037675_0_0_1 |
InParanoidi | Q8CCN5 |
OrthoDBi | 537714at2759 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR022175, BCAS3 IPR045142, BCAS3-like IPR015943, WD40/YVTN_repeat-like_dom_sf IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR13268, PTHR13268, 1 hit |
Pfami | View protein in Pfam PF12490, BCAS3, 1 hit |
SUPFAMi | SSF50978, SSF50978, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNETMATDSP RRPSRCTGGV VVRPQAVTEQ SYMESVVTFL QDVVPQAYSG
60 70 80 90 100
SPLTEEKEKI VWVRFENADL NDTSRNLEFH ELHSTGNEPP LLVMIGYSDG
110 120 130 140 150
MQVWGIPISG EAQELFSVRH GPVRAARILP APQLGAQKCD NFAEKRPLLG
160 170 180 190 200
VCKSIGSSGT TPPYCCVDLY SLRTGEMVKS IQFKTPIYDL HCNKRILVVV
210 220 230 240 250
LQEKIAAFDS CTFTKKFFVT SCYPCPGPNM NPIALGSRWL AYAENKLIRC
260 270 280 290 300
HQSRGGACGD NIQSYTATVL SAAKTLKSGL TMVGKVVTQL TGTLPSGVTE
310 320 330 340 350
DDVALHCNSR RSPLVPGIIT VIDTETVGEG QVLVSEDSDS DGIVAHFPAH
360 370 380 390 400
EKPVCCMAFN TSGMLLVTTD TLGHDFHVFQ ILTHPWSSSQ CAVHHLYTLH
410 420 430 440 450
RGETEAKVQD ICFSHDCRWV VVSTLRGTSH VFPINPYGGQ PCVRTHMSPR
460 470 480 490 500
VVNRMSRFQK SAGLEEIEQE LTSKQGGRCS PVPGLSSSPS GSPLHGKLTS
510 520 530 540 550
QDSYNNFTNN NPGNPRLSPL PSLMVVTPLA QIKQPMTLGT ITKRTGPYLF
560 570 580 590 600
GAGCFSIKAP CKVKSPPQIS PSKSMGGEFC VAAVFGTSRS WFANNAGLKR
610 620 630 640 650
EKDQSKQVVV ESLYIISCYG TLVEHMIEPR PISTAPKISD DTPLEIMTSP
660 670 680 690 700
RASWTLVRTP QWNELQPPFN ANHPLLLAAE AVQYYQLLLA GSLPPGSPGP
710 720 730 740 750
ITRHGSYDSL ASDHSGQEDE EWLSQVEIVT HTGPHRRLWM GPQFHFKTIQ
760 770 780 790 800
TSGQTTVIST SSSVLQSHGP SDTPQPLLDF DTDDLDLNSL RIQPVRSDPV
810 820 830 840 850
SMPGSSRAVS DRRGVSTVTD AASGTFDRSV TLLEVCGSWP EGFGLRHMSS
860 870 880 890 900
MEHSEEGLRE RLADAMAESP SRDVVGSGTE LQREGSIETL SNSSGSTSGS
910 920
IPRNFDGYRS PLPTNESQPL SLFPTGFP
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB1AR74 | B1AR74_MOUSE | BCAS3 microtubule-associated cell m... | Bcas3 | 943 | Annotation score: | ||
Q0VF62 | Q0VF62_MOUSE | BCAS3 microtubule-associated cell m... | Bcas3 | 913 | Annotation score: | ||
F7C0F2 | F7C0F2_MOUSE | BCAS3 microtubule-associated cell m... | Bcas3 | 693 | Annotation score: | ||
F7CPM1 | F7CPM1_MOUSE | BCAS3 microtubule-associated cell m... | Bcas3 | 477 | Annotation score: | ||
B1AR76 | B1AR76_MOUSE | BCAS3 microtubule-associated cell m... | Bcas3 | 458 | Annotation score: | ||
F6VJ09 | F6VJ09_MOUSE | BCAS3 microtubule-associated cell m... | Bcas3 | 86 | Annotation score: | ||
Q5SWI1 | Q5SWI1_MOUSE | BCAS3 microtubule-associated cell m... | Bcas3 | 165 | Annotation score: | ||
B1B0B3 | B1B0B3_MOUSE | BCAS3 microtubule-associated cell m... | Bcas3 | 120 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 75 | R → Q in BAC27862 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 472 | T → D (PubMed:16099728).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007861 | 498 – 527 | LTSQD…LMVVT → HFPLMLLSSRFLLYHLGSDA NFYSVCAEHS in isoform 2. 1 PublicationAdd BLAST | 30 | |
Alternative sequenceiVSP_007862 | 528 – 928 | Missing in isoform 2. 1 PublicationAdd BLAST | 401 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF313800 mRNA Translation: AAG34697.1 Sequence problems. AK032423 mRNA Translation: BAC27862.1 AK034117 mRNA Translation: BAC28592.1 |
CCDSi | CCDS25195.1 [Q8CCN5-1] |
RefSeqi | NP_001160114.1, NM_001166642.1 NP_619622.3, NM_138681.4 [Q8CCN5-1] XP_006532603.1, XM_006532540.2 [Q8CCN5-1] |
Genome annotation databases
Ensembli | ENSMUST00000074875; ENSMUSP00000074416; ENSMUSG00000059439 [Q8CCN5-1] ENSMUST00000108056; ENSMUSP00000103691; ENSMUSG00000059439 [Q8CCN5-2] |
GeneIDi | 192197 |
KEGGi | mmu:192197 |
UCSCi | uc007krn.2, mouse [Q8CCN5-2] uc007krp.2, mouse [Q8CCN5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF313800 mRNA Translation: AAG34697.1 Sequence problems. AK032423 mRNA Translation: BAC27862.1 AK034117 mRNA Translation: BAC28592.1 |
CCDSi | CCDS25195.1 [Q8CCN5-1] |
RefSeqi | NP_001160114.1, NM_001166642.1 NP_619622.3, NM_138681.4 [Q8CCN5-1] XP_006532603.1, XM_006532540.2 [Q8CCN5-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 228674, 2 interactors |
STRINGi | 10090.ENSMUSP00000103696 |
PTM databases
iPTMneti | Q8CCN5 |
PhosphoSitePlusi | Q8CCN5 |
SwissPalmi | Q8CCN5 |
Proteomic databases
EPDi | Q8CCN5 |
jPOSTi | Q8CCN5 |
MaxQBi | Q8CCN5 |
PaxDbi | Q8CCN5 |
PeptideAtlasi | Q8CCN5 |
PRIDEi | Q8CCN5 |
ProteomicsDBi | 273734 [Q8CCN5-1] 273735 [Q8CCN5-2] |
Protocols and materials databases
Antibodypediai | 9303, 225 antibodies from 36 providers |
Genome annotation databases
Ensembli | ENSMUST00000074875; ENSMUSP00000074416; ENSMUSG00000059439 [Q8CCN5-1] ENSMUST00000108056; ENSMUSP00000103691; ENSMUSG00000059439 [Q8CCN5-2] |
GeneIDi | 192197 |
KEGGi | mmu:192197 |
UCSCi | uc007krn.2, mouse [Q8CCN5-2] uc007krp.2, mouse [Q8CCN5-1] |
Organism-specific databases
CTDi | 54828 |
MGIi | MGI:2385848, Bcas3 |
VEuPathDBi | HostDB:ENSMUSG00000059439 |
Phylogenomic databases
eggNOGi | KOG2109, Eukaryota KOG4415, Eukaryota |
GeneTreei | ENSGT00390000006454 |
HOGENOMi | CLU_037675_0_0_1 |
InParanoidi | Q8CCN5 |
OrthoDBi | 537714at2759 |
Miscellaneous databases
BioGRID-ORCSi | 192197, 1 hit in 64 CRISPR screens |
ChiTaRSi | Bcas3, mouse |
PROi | PR:Q8CCN5 |
RNActi | Q8CCN5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000059439, Expressed in brain and 275 other tissues |
ExpressionAtlasi | Q8CCN5, baseline and differential |
Genevisiblei | Q8CCN5, MM |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR022175, BCAS3 IPR045142, BCAS3-like IPR015943, WD40/YVTN_repeat-like_dom_sf IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR13268, PTHR13268, 1 hit |
Pfami | View protein in Pfam PF12490, BCAS3, 1 hit |
SUPFAMi | SSF50978, SSF50978, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BCAS3_MOUSE | |
Accessioni | Q8CCN5Primary (citable) accession number: Q8CCN5 Secondary accession number(s): Q8CC16, Q9EPX3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2003 |
Last sequence update: | July 25, 2003 | |
Last modified: | February 23, 2022 | |
This is version 147 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families