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Protein

U4/U6 small nuclear ribonucleoprotein Prp31

Gene

Prpf31

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing as component of the spliceosome. Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6 small nuclear ribonucleoprotein Prp31
Alternative name(s):
Pre-mRNA-processing factor 31
U4/U6 snRNP 61 kDa protein
Short name:
Protein 61K
Gene namesi
Name:Prpf31
Synonyms:Prp31
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1916238 Prpf31

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002278001 – 499U4/U6 small nuclear ribonucleoprotein Prp31Add BLAST499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei379PhosphoserineBy similarity1
Modified residuei395PhosphoserineBy similarity1
Modified residuei432PhosphoserineBy similarity1
Modified residuei438N6-acetyllysineCombined sources1
Modified residuei439PhosphoserineBy similarity1
Modified residuei440PhosphothreonineBy similarity1
Modified residuei450PhosphoserineBy similarity1
Modified residuei455PhosphothreonineCombined sources1
Cross-linki471Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki478Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8CCF0
PeptideAtlasiQ8CCF0
PRIDEiQ8CCF0

PTM databases

iPTMnetiQ8CCF0
PhosphoSitePlusiQ8CCF0

Expressioni

Gene expression databases

BgeeiENSMUSG00000008373 Expressed in 283 organ(s), highest expression level in primitive streak
GenevisibleiQ8CCF0 MM

Interactioni

Subunit structurei

Identified in the spliceosome B complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Interacts with a complex formed by SNU13 and U4 snRNA, but not with SNU13 or U4 snRNA alone. The complex formed by SNU13 and PRPF31 binds also U4atac snRNA, a characteristic component of specific, less abundant spliceosomal complexes. Interacts with PRPF6/U5 snRNP-associated 102 kDa protein. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts (via its NLS) with CTNNBL1.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei247Interaction with U4 snRNABy similarity1
Sitei270Interaction with U4 snRNA and U4atac snRNABy similarity1
Sitei289Interaction with U4atac snRNABy similarity1
Sitei293Interaction with U4 snRNA and U4atac snRNABy similarity1
Sitei298Interaction with U4 snRNA and U4atac snRNABy similarity1

Protein-protein interaction databases

BioGridi213160, 3 interactors
IntActiQ8CCF0, 3 interactors
MINTiQ8CCF0
STRINGi10090.ENSMUSP00000008517

Structurei

3D structure databases

ProteinModelPortaliQ8CCF0
SMRiQ8CCF0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini215 – 333NopPROSITE-ProRule annotationAdd BLAST119

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili85 – 120By similarityAdd BLAST36
Coiled coili181 – 215By similarityAdd BLAST35

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi351 – 364Nuclear localization signal (NLS)By similarityAdd BLAST14

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi16 – 19Poly-Glu4
Compositional biasi25 – 29Poly-Glu5

Domaini

Interacts with the snRNP via the Nop domain.By similarity
The coiled coil domain is formed by two non-contiguous helices.By similarity

Sequence similaritiesi

Belongs to the PRP31 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2574 Eukaryota
COG1498 LUCA
GeneTreeiENSGT00550000075069
HOVERGENiHBG082193
InParanoidiQ8CCF0
KOiK12844
OMAiFTRDKYS
OrthoDBiEOG091G09U7
TreeFamiTF300677

Family and domain databases

Gene3Di1.10.287.660, 1 hit
InterProiView protein in InterPro
IPR029012 Helix_hairpin_bin_sf
IPR002687 Nop_dom
IPR036070 Nop_dom_sf
IPR012976 NOSIC
IPR027105 Prp31
IPR019175 Prp31_C
PANTHERiPTHR13904 PTHR13904, 1 hit
PfamiView protein in Pfam
PF01798 Nop, 1 hit
PF09785 Prp31_C, 1 hit
SMARTiView protein in SMART
SM00931 NOSIC, 1 hit
SUPFAMiSSF89124 SSF89124, 1 hit
PROSITEiView protein in PROSITE
PS51358 NOP, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q8CCF0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSLADELLAD LEEAAEEEEG GSYGEEEEEP AIEDVQEETQ LDLSGDSVKS
60 70 80 90 100
IAKLWDSKMF AEIMMKIEEY ISKQANVSEV MGPVEAAPEY RVIVDANNLT
110 120 130 140 150
VEIENELNII HKFIRDKYSK RFPELESLVP NALDYIRTVK ELGNSLDKCK
160 170 180 190 200
NNENLQQILT NATIMVVSVT ASTTQGQQLS DEELERLEEA CDMALELNAS
210 220 230 240 250
KHRIYEYVES RMSFIAPNLS IIIGASTAAK IMGVAGGLTN LSKMPACNIM
260 270 280 290 300
LLGAQRKTLS GFSSTSVLPH TGYIYHSDIV QSLPPDLRRK AARLVAAKCT
310 320 330 340 350
LAARVDSFHE STEGKVGYEL KDEIERKFDK WQEPPPVKQV KPLPAPLDGQ
360 370 380 390 400
RKKRGGRRYR KMKERLGLTE IRKQANRMSF GEIEEDAYQE DLGFSLGHLG
410 420 430 440 450
KSGSGRVRQT QVNEATKARI SKTLQRTLQK QSVVYGGKST IRDRSSGTAS
460 470 480 490
SVAFTPLQGL EIVNPQAAEK KVAEANQKYF SSMAEFLKVK GEKSGTMST
Length:499
Mass (Da):55,430
Last modified:July 27, 2011 - v3
Checksum:iA8149257A6213D4F
GO
Isoform 2 (identifier: Q8CCF0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     316-321: Missing.

Note: No experimental confirmation available.
Show »
Length:493
Mass (Da):54,740
Checksum:i4D95CBC24749FA1A
GO
Isoform 4 (identifier: Q8CCF0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     60-65: FAEIMM → VSLLRS
     66-499: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
Show »
Length:65
Mass (Da):7,146
Checksum:iFE35F67E54C4EF98
GO

Sequence cautioni

The sequence BAC25109 differs from that shown. Reason: Frameshift at position 357.Curated
The sequence BAC31903 differs from that shown. Contaminating sequence. The C-terminus matches chromosome 19 region.Curated
The sequence BAC31931 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated
The sequence BAC34578 differs from that shown. Reason: Frameshift at position 7.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77V → A in AAK77987 (PubMed:11867543).Curated1
Sequence conflicti77V → A in BAC31931 (PubMed:16141072).Curated1
Sequence conflicti77V → A in CAJ18397 (Ref. 3) Curated1
Sequence conflicti77V → A in AAH18376 (PubMed:15489334).Curated1
Sequence conflicti77V → A in AAH57877 (PubMed:15489334).Curated1
Sequence conflicti104E → V in BAC28192 (PubMed:16141072).Curated1
Sequence conflicti177Q → R in BAC28220 (PubMed:16141072).Curated1
Sequence conflicti177Q → R in BAC28192 (PubMed:16141072).Curated1
Sequence conflicti382E → G in BAC25109 (PubMed:16141072).Curated1
Sequence conflicti481S → F in AAH61461 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01758960 – 65FAEIMM → VSLLRS in isoform 4. 1 Publication6
Alternative sequenceiVSP_01759066 – 499Missing in isoform 4. 1 PublicationAdd BLAST434
Alternative sequenceiVSP_017591316 – 321Missing in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY040823 mRNA Translation: AAK77987.1
AK005294 mRNA Translation: BAC25109.1 Frameshift.
AK033190 mRNA Translation: BAC28192.1
AK033283 mRNA Translation: BAC28220.1
AK044398 mRNA Translation: BAC31903.1 Sequence problems.
AK044457 mRNA Translation: BAC31931.1 Sequence problems.
AK051260 mRNA Translation: BAC34578.1 Frameshift.
CT010189 mRNA Translation: CAJ18397.1
AC130680 Genomic DNA No translation available.
BC018376 mRNA Translation: AAH18376.1
BC057877 mRNA Translation: AAH57877.1
BC061461 mRNA Translation: AAH61461.1
CCDSiCCDS39729.1 [Q8CCF0-1]
CCDS51965.1 [Q8CCF0-2]
RefSeqiNP_001153186.1, NM_001159714.1 [Q8CCF0-2]
NP_081604.3, NM_027328.4 [Q8CCF0-1]
UniGeneiMm.246863

Genome annotation databases

EnsembliENSMUST00000008517; ENSMUSP00000008517; ENSMUSG00000008373 [Q8CCF0-1]
ENSMUST00000108636; ENSMUSP00000104276; ENSMUSG00000008373 [Q8CCF0-2]
ENSMUST00000125782; ENSMUSP00000146017; ENSMUSG00000008373 [Q8CCF0-4]
ENSMUST00000179769; ENSMUSP00000136031; ENSMUSG00000008373 [Q8CCF0-2]
GeneIDi68988
KEGGimmu:68988
UCSCiuc009evi.2 mouse [Q8CCF0-1]
uc012ewf.1 mouse [Q8CCF0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY040823 mRNA Translation: AAK77987.1
AK005294 mRNA Translation: BAC25109.1 Frameshift.
AK033190 mRNA Translation: BAC28192.1
AK033283 mRNA Translation: BAC28220.1
AK044398 mRNA Translation: BAC31903.1 Sequence problems.
AK044457 mRNA Translation: BAC31931.1 Sequence problems.
AK051260 mRNA Translation: BAC34578.1 Frameshift.
CT010189 mRNA Translation: CAJ18397.1
AC130680 Genomic DNA No translation available.
BC018376 mRNA Translation: AAH18376.1
BC057877 mRNA Translation: AAH57877.1
BC061461 mRNA Translation: AAH61461.1
CCDSiCCDS39729.1 [Q8CCF0-1]
CCDS51965.1 [Q8CCF0-2]
RefSeqiNP_001153186.1, NM_001159714.1 [Q8CCF0-2]
NP_081604.3, NM_027328.4 [Q8CCF0-1]
UniGeneiMm.246863

3D structure databases

ProteinModelPortaliQ8CCF0
SMRiQ8CCF0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213160, 3 interactors
IntActiQ8CCF0, 3 interactors
MINTiQ8CCF0
STRINGi10090.ENSMUSP00000008517

PTM databases

iPTMnetiQ8CCF0
PhosphoSitePlusiQ8CCF0

Proteomic databases

PaxDbiQ8CCF0
PeptideAtlasiQ8CCF0
PRIDEiQ8CCF0

Protocols and materials databases

DNASUi68988
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000008517; ENSMUSP00000008517; ENSMUSG00000008373 [Q8CCF0-1]
ENSMUST00000108636; ENSMUSP00000104276; ENSMUSG00000008373 [Q8CCF0-2]
ENSMUST00000125782; ENSMUSP00000146017; ENSMUSG00000008373 [Q8CCF0-4]
ENSMUST00000179769; ENSMUSP00000136031; ENSMUSG00000008373 [Q8CCF0-2]
GeneIDi68988
KEGGimmu:68988
UCSCiuc009evi.2 mouse [Q8CCF0-1]
uc012ewf.1 mouse [Q8CCF0-2]

Organism-specific databases

CTDi26121
MGIiMGI:1916238 Prpf31

Phylogenomic databases

eggNOGiKOG2574 Eukaryota
COG1498 LUCA
GeneTreeiENSGT00550000075069
HOVERGENiHBG082193
InParanoidiQ8CCF0
KOiK12844
OMAiFTRDKYS
OrthoDBiEOG091G09U7
TreeFamiTF300677

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway

Miscellaneous databases

PROiPR:Q8CCF0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000008373 Expressed in 283 organ(s), highest expression level in primitive streak
GenevisibleiQ8CCF0 MM

Family and domain databases

Gene3Di1.10.287.660, 1 hit
InterProiView protein in InterPro
IPR029012 Helix_hairpin_bin_sf
IPR002687 Nop_dom
IPR036070 Nop_dom_sf
IPR012976 NOSIC
IPR027105 Prp31
IPR019175 Prp31_C
PANTHERiPTHR13904 PTHR13904, 1 hit
PfamiView protein in Pfam
PF01798 Nop, 1 hit
PF09785 Prp31_C, 1 hit
SMARTiView protein in SMART
SM00931 NOSIC, 1 hit
SUPFAMiSSF89124 SSF89124, 1 hit
PROSITEiView protein in PROSITE
PS51358 NOP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPRP31_MOUSE
AccessioniPrimary (citable) accession number: Q8CCF0
Secondary accession number(s): E9QPM6
, Q6P7X2, Q8BQ91, Q8C8U4, Q8C8V5, Q8CCG6, Q8CF52, Q8VBW3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 132 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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