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Protein

Transcription factor E4F1

Gene

E4f1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription.4 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri193 – 215C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri221 – 243C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri249 – 273C2H2-type 3; degeneratePROSITE-ProRule annotationAdd BLAST25
Zinc fingeri434 – 456C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri462 – 484C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri490 – 512C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri518 – 540C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri546 – 568C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri574 – 596C2H2-type 9; degeneratePROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor, Transferase
Biological processCell cycle, Cell division, Growth regulation, Mitosis, Transcription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E4F1 (EC:2.3.2.27By similarity)
Alternative name(s):
E4F transcription factor 1
Putative E3 ubiquitin-protein ligase E4F1
RING-type E3 ubiquitin transferase E4F1Curated
Transcription factor E4F
Transcription factor phi AP3
p120E4F
Gene namesi
Name:E4f1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:109530 E4f1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Death before E7.5 probably at the peri-implantation stage. Blastocysts display defects in mitotic progression and chromosomal segregation and increased apoptosis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003243081 – 783Transcription factor E4F1Add BLAST783

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated; phosphorylation is cell cycle-dependent and regulates DNA-binding activity and function.1 Publication
May be sumoylated by UBE2I upon interaction with CDKN2A.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8CCE9
PRIDEiQ8CCE9

PTM databases

iPTMnetiQ8CCE9
PhosphoSitePlusiQ8CCE9

Expressioni

Tissue specificityi

Ubiquitously expressed.2 Publications

Developmental stagei

Continuously expressed during embryogenesis.1 Publication

Gene expression databases

BgeeiENSMUSG00000024137 Expressed in 302 organ(s), highest expression level in head
CleanExiMM_E4F1
GenevisibleiQ8CCE9 MM

Interactioni

Subunit structurei

Homodimer; binds DNA as a dimer (By similarity). Forms a complex with CDKN2A and TP53. Interacts with HDAC1, HMGA2 and RASSF1 (By similarity). Interactions with TP53, RB1, ANP32A and probably BMI1 and FHL2 regulate E4F1 activity.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Anp32aO353812EBI-7450874,EBI-643140

Protein-protein interaction databases

BioGridi199353, 4 interactors
IntActiQ8CCE9, 1 interactor
MINTiQ8CCE9
STRINGi10090.ENSMUSP00000062344

Structurei

3D structure databases

ProteinModelPortaliQ8CCE9
SMRiQ8CCE9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 84Required for ubiquitin ligase activityBy similarityAdd BLAST45
Regioni185 – 264Mediates dimerization, DNA-binding, transcription repression of CCNA2 and interaction with HMGA2By similarityAdd BLAST80
Regioni368 – 565Mediates interaction with CDKN2ABy similarityAdd BLAST198
Regioni434 – 598Interaction with BMI1By similarityAdd BLAST165
Regioni520 – 579Mediates interaction with TP53By similarityAdd BLAST60
Regioni574 – 596Mediates interaction with RASSF1By similarityAdd BLAST23

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri193 – 215C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri221 – 243C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri249 – 273C2H2-type 3; degeneratePROSITE-ProRule annotationAdd BLAST25
Zinc fingeri434 – 456C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri462 – 484C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri490 – 512C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri518 – 540C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri546 – 568C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri574 – 596C2H2-type 9; degeneratePROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00840000129970
HOVERGENiHBG052707
InParanoidiQ8CCE9
KOiK22401
OMAiSGHQIIV
OrthoDBiEOG091G03X5
PhylomeDBiQ8CCE9
TreeFamiTF315387

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 4 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 10 hits
SUPFAMiSSF57667 SSF57667, 6 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 7 hits
PS50157 ZINC_FINGER_C2H2_2, 9 hits

Sequences (5+)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8CCE9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEGAMAVRVT AAHTAEARAE AGREAGEGGV AAAAALSSGG FLGLPAPFSE
60 70 80 90 100
EDEDDVHRCG RCQVEFTALE DFVQHKIQKT CHRAPQEALP TTPAATALLD
110 120 130 140 150
QEVVPTAAEG GPDEPITVAH IVVEATSLAE DISHAPDLVG SGHIKEVIVA
160 170 180 190 200
AEAEPGDVEM AEAPGSPNHQ ELGLLGEGEQ AHVKLLVNKE GRYVCMLCHK
210 220 230 240 250
TFKTGSILKA HMVTHSSRKD HECKLCGASF RTKGSLIRHH RRHTDERPYK
260 270 280 290 300
CAKCGKSFRE SGALTRHLKS LTPCTEKIRF SISKDTAVGK EEVPAGSSAS
310 320 330 340 350
TVGTVTSSVA GDPMETSPVI HLVTDAKGTV IHEVHVQMQE LPLGMKALTP
360 370 380 390 400
ESPDSEELPC SSENSRENLL HQAMQNSGIV LERVAGEESA LEPAPPSGSS
410 420 430 440 450
PQCLGDGSPE LPLLKVEQIE TQVASEAATV PRTHPCPQCS ETFPTAATLE
460 470 480 490 500
AHKRGHIAPR PFTCTQCGKA FPKAYLLKKH QEVHVHERRF RCGDCGKLYK
510 520 530 540 550
TIAHVRGHRR VHSDERPFPC PQCGKRYKTK NAQQVHFRTH LEEKPHVCQF
560 570 580 590 600
CSRGFREKGS LVRHVRHHTG EKPFKCYKCG RGFAEHGTLN RHLRTKGGCL
610 620 630 640 650
LEVEELLVSE ESPSAAATVL AEDPHTVLVE FSSVVADTQE YIIEATADDT
660 670 680 690 700
ETSEATEIIE GTQTEVDSHI MKVVQQIVHQ AGAGHQIIVQ NVTMDQETAL
710 720 730 740 750
GSEATAADTI TIATPESLTE QVAMTLASAI SEGTVLTARA GPNSTEQATV
760 770 780
TMVSSEDIEI LEHGGELVIA SPEGQLEVQT VIV
Length:783
Mass (Da):84,296
Last modified:March 18, 2008 - v2
Checksum:i59E47F7A64A224A4
GO
Isoform 2 (identifier: Q8CCE9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     667-684: DSHIMKVVQQIVHQAGAG → RVWVRDGRITGLLGSPSG
     685-783: Missing.

Show »
Length:684
Mass (Da):74,060
Checksum:i62B9E2973ACA07C3
GO
Isoform 3 (identifier: Q8CCE9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     422-422: Missing.

Note: No experimental confirmation available.
Show »
Length:782
Mass (Da):84,168
Checksum:iF0CEA4AC61FE5BDA
GO
Isoform 4 (identifier: Q8CCE9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     458-458: A → VWQGLPQSLP...AWHTQPAPAH
     459-783: Missing.

Note: No experimental confirmation available.
Show »
Length:586
Mass (Da):62,553
Checksum:i03DE21745C393119
GO
Isoform 5 (identifier: Q8CCE9-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     244-274: Missing.

Note: No experimental confirmation available.
Show »
Length:752
Mass (Da):80,830
Checksum:iBC7A27C32B98E325
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2I3BRE9A0A2I3BRE9_MOUSE
Transcription factor E4F1
E4f1
427Annotation score:
A0A2I3BPE3A0A2I3BPE3_MOUSE
Transcription factor E4F1
E4f1
586Annotation score:
A0A2I3BR50A0A2I3BR50_MOUSE
Transcription factor E4F1
E4f1
675Annotation score:

Sequence cautioni

The sequence AAF22563 differs from that shown. Reason: Frameshift at positions 230 and 235.Curated
The sequence AAH71228 differs from that shown. Reason: Frameshift at position 94.Curated
The sequence BAE25748 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA54188 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13H → D in BAE25748 (PubMed:16141072).Curated1
Sequence conflicti18R → G in AAF22563 (Ref. 1) Curated1
Sequence conflicti18R → G in BAE25748 (PubMed:16141072).Curated1
Sequence conflicti18R → G in AAH46459 (PubMed:15489334).Curated1
Sequence conflicti18R → G in AAH71228 (PubMed:15489334).Curated1
Sequence conflicti30V → VA in AAF22563 (Ref. 1) Curated1
Sequence conflicti30V → VA in BAE25748 (PubMed:16141072).Curated1
Sequence conflicti62C → F in BAE25748 (PubMed:16141072).Curated1
Sequence conflicti139V → A in CAA54188 (PubMed:8262041).Curated1
Sequence conflicti148I → V in CAA54188 (PubMed:8262041).Curated1
Sequence conflicti158V → G in AAF22563 (Ref. 1) Curated1
Sequence conflicti158V → G in BAE25748 (PubMed:16141072).Curated1
Sequence conflicti158V → G in AAH46459 (PubMed:15489334).Curated1
Sequence conflicti158V → G in AAH71228 (PubMed:15489334).Curated1
Sequence conflicti158V → G in CAA54188 (PubMed:8262041).Curated1
Sequence conflicti224 – 225KL → NF in AAF22563 (Ref. 1) Curated2
Sequence conflicti281S → N in AAF22563 (Ref. 1) Curated1
Sequence conflicti281S → N in BAE25748 (PubMed:16141072).Curated1
Sequence conflicti380V → L in AAF22563 (Ref. 1) Curated1
Sequence conflicti390A → V in AAF22563 (Ref. 1) Curated1
Sequence conflicti395 – 400PPSGSS → LPFGST in AAF22563 (Ref. 1) Curated6
Sequence conflicti412P → L in AAF22563 (Ref. 1) Curated1
Sequence conflicti479 – 480KH → ND in CAA54188 (PubMed:8262041).Curated2
Sequence conflicti585 – 586EH → DD in CAA54188 (PubMed:8262041).Curated2
Sequence conflicti630E → Q in AAF22563 (Ref. 1) Curated1
Sequence conflicti630E → Q in BAE25748 (PubMed:16141072).Curated1
Sequence conflicti630E → Q in AAH46459 (PubMed:15489334).Curated1
Sequence conflicti630E → Q in CAA54188 (PubMed:8262041).Curated1
Sequence conflicti705T → A in AAF22563 (Ref. 1) Curated1
Sequence conflicti705T → A in BAE25748 (PubMed:16141072).Curated1
Sequence conflicti705T → A in CAA54188 (PubMed:8262041).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_032192244 – 274Missing in isoform 5. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_032193422Missing in isoform 3. 1 Publication1
Alternative sequenceiVSP_032194458A → VWQGLPQSLPAQEAPGGARA RAPLPLWRLWEALQDHRSCA GPPACSLRREAFPLSPVRQA LQNQECPASTLPDTSGRKAP RVPVLQPRLPGEGLSGAACE APHRRETFQVLQVWPWLRGA WHTQPAPAH in isoform 4. 1 Publication1
Alternative sequenceiVSP_032195459 – 783Missing in isoform 4. 1 PublicationAdd BLAST325
Alternative sequenceiVSP_032196667 – 684DSHIM…QAGAG → RVWVRDGRITGLLGSPSG in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_032197685 – 783Missing in isoform 2. 1 PublicationAdd BLAST99

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126967 mRNA Translation: AAF22563.1 Frameshift.
AK033285 mRNA Translation: BAC28222.1
AK144176 mRNA Translation: BAE25748.1 Different initiation.
BC046459 mRNA Translation: AAH46459.1
BC057011 mRNA Translation: AAH57011.1
BC071228 mRNA Translation: AAH71228.1 Frameshift.
X76858 mRNA Translation: CAA54188.1 Different initiation.
CCDSiCCDS28481.1 [Q8CCE9-3]
PIRiS41688
RefSeqiNP_001288713.1, NM_001301784.1 [Q8CCE9-1]
NP_031919.2, NM_007893.4 [Q8CCE9-3]
XP_011244570.1, XM_011246268.2 [Q8CCE9-5]
UniGeneiMm.163132
Mm.445985

Genome annotation databases

EnsembliENSMUST00000056032; ENSMUSP00000062344; ENSMUSG00000024137 [Q8CCE9-3]
ENSMUST00000226941; ENSMUSP00000154444; ENSMUSG00000024137 [Q8CCE9-2]
GeneIDi13560
KEGGimmu:13560
UCSCiuc008avy.2 mouse [Q8CCE9-3]
uc008awa.2 mouse [Q8CCE9-1]
uc008awb.2 mouse [Q8CCE9-2]
uc012ami.2 mouse [Q8CCE9-5]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126967 mRNA Translation: AAF22563.1 Frameshift.
AK033285 mRNA Translation: BAC28222.1
AK144176 mRNA Translation: BAE25748.1 Different initiation.
BC046459 mRNA Translation: AAH46459.1
BC057011 mRNA Translation: AAH57011.1
BC071228 mRNA Translation: AAH71228.1 Frameshift.
X76858 mRNA Translation: CAA54188.1 Different initiation.
CCDSiCCDS28481.1 [Q8CCE9-3]
PIRiS41688
RefSeqiNP_001288713.1, NM_001301784.1 [Q8CCE9-1]
NP_031919.2, NM_007893.4 [Q8CCE9-3]
XP_011244570.1, XM_011246268.2 [Q8CCE9-5]
UniGeneiMm.163132
Mm.445985

3D structure databases

ProteinModelPortaliQ8CCE9
SMRiQ8CCE9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199353, 4 interactors
IntActiQ8CCE9, 1 interactor
MINTiQ8CCE9
STRINGi10090.ENSMUSP00000062344

PTM databases

iPTMnetiQ8CCE9
PhosphoSitePlusiQ8CCE9

Proteomic databases

PaxDbiQ8CCE9
PRIDEiQ8CCE9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056032; ENSMUSP00000062344; ENSMUSG00000024137 [Q8CCE9-3]
ENSMUST00000226941; ENSMUSP00000154444; ENSMUSG00000024137 [Q8CCE9-2]
GeneIDi13560
KEGGimmu:13560
UCSCiuc008avy.2 mouse [Q8CCE9-3]
uc008awa.2 mouse [Q8CCE9-1]
uc008awb.2 mouse [Q8CCE9-2]
uc012ami.2 mouse [Q8CCE9-5]

Organism-specific databases

CTDi1877
MGIiMGI:109530 E4f1

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00840000129970
HOVERGENiHBG052707
InParanoidiQ8CCE9
KOiK22401
OMAiSGHQIIV
OrthoDBiEOG091G03X5
PhylomeDBiQ8CCE9
TreeFamiTF315387

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

PROiPR:Q8CCE9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024137 Expressed in 302 organ(s), highest expression level in head
CleanExiMM_E4F1
GenevisibleiQ8CCE9 MM

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 4 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 10 hits
SUPFAMiSSF57667 SSF57667, 6 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 7 hits
PS50157 ZINC_FINGER_C2H2_2, 9 hits
ProtoNetiSearch...

Entry informationi

Entry nameiE4F1_MOUSE
AccessioniPrimary (citable) accession number: Q8CCE9
Secondary accession number(s): Q05BH7
, Q3UNJ9, Q62065, Q6IR08, Q6PGI1, Q9QY56
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: November 7, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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