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Protein

Nicotinate phosphoribosyltransferase

Gene

Naprt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate. Helps prevent cellular oxidative stress via its role in NAD biosynthesis.By similarity

Catalytic activityi

Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate.By similarity

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Activity is highest with Mn2+.By similarity

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate D-ribonucleotide from nicotinate.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinate phosphoribosyltransferase (Naprt)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate D-ribonucleotide from nicotinate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21NicotinateBy similarity1
Binding sitei210NicotinateBy similarity1
Binding sitei318NicotinateBy similarity1
Binding sitei380PhosphoribosylpyrophosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase, Transferase
Biological processPyridine nucleotide biosynthesis
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi6.3.4.21 3474
ReactomeiR-MMU-197264 Nicotinamide salvaging
R-MMU-6798695 Neutrophil degranulation
UniPathwayi
UPA00253;UER00457

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate phosphoribosyltransferase (EC:6.3.4.21By similarity)
Short name:
NAPRTase
Alternative name(s):
Nicotinate phosphoribosyltransferase domain-containing protein 1
Gene namesi
Name:Naprt
Synonyms:Naprt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2442664 Naprt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003156821 – 538Nicotinate phosphoribosyltransferaseAdd BLAST538

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei213PhosphohistidineBy similarity1

Post-translational modificationi

Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8CC86
PaxDbiQ8CC86
PeptideAtlasiQ8CC86
PRIDEiQ8CC86

PTM databases

iPTMnetiQ8CC86
PhosphoSitePlusiQ8CC86
SwissPalmiQ8CC86

Expressioni

Tissue specificityi

Abundantly expressed in the small intestine, liver and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000022574 Expressed in 155 organ(s), highest expression level in intestine
GenevisibleiQ8CC86 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ8CC86, 3 interactors
MINTiQ8CC86
STRINGi10090.ENSMUSP00000023237

Structurei

3D structure databases

ProteinModelPortaliQ8CC86
SMRiQ8CC86
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAPRTase family.Curated

Phylogenomic databases

eggNOGiKOG2511 Eukaryota
COG1488 LUCA
GeneTreeiENSGT00390000002664
HOGENOMiHOG000001111
HOVERGENiHBG062439
InParanoidiQ8CC86
KOiK00763
OMAiNPHIYKV
OrthoDBiEOG091G088G
PhylomeDBiQ8CC86
TreeFamiTF314732

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR007229 Nic_PRibTrfase-Fam
IPR006405 Nic_PRibTrfase_pncB
IPR036068 Nicotinate_pribotase_like_C
PANTHERiPTHR11098 PTHR11098, 1 hit
PIRSFiPIRSF000484 NAPRT, 1 hit
SUPFAMiSSF51690 SSF51690, 2 hits
TIGRFAMsiTIGR01513 NAPRTase_put, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8CC86-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEMELDSEGR MVVRPLLTDL YQATMALGYW RAGRACEAAE FELFFRHCPF
60 70 80 90 100
GGSFALSAGL QDCMRFLRAF RLRDADVQFL ASVLPPDTDP AFFEHLRALD
110 120 130 140 150
CSGVTVRALP EGSLAFPGVP LLQVSGPLLL VQLLETPLLC LVSYASLVAT
160 170 180 190 200
NAARLRLIAG PDKKLLEMGL RRAQGPDGGF TASIYSYLGG FDSSSNTLAG
210 220 230 240 250
QLRGVPVAGT LAHSFVTSFS GSEVPPDPML APASSEGPTV DLPARVNLWL
260 270 280 290 300
KRVCLYLGLE EQEPHPGERA AFVAYALAFP RAFQGLLDSY SVRRSGLPNF
310 320 330 340 350
LAVALALGEL GYRAVGVRLD SGDLLQQAKE IRGIFRTAGA QFQMPWLESV
360 370 380 390 400
PIAVSNNIDE SELMRLAQKG SEVNVIGIGT SVVTCPKQPS MGCVYKLVSV
410 420 430 440 450
GGQPRIKLTE DPEKQTLPGS KAAFRFLGPD GSLLLDLLQL AEEPPPKAGQ
460 470 480 490 500
ELRVWPRGTQ EPCTVKPAQV EPLLRLYLQQ GQLCEPLPSL DESRRFAQQS
510 520 530
LSLLRPAHKQ LQSPAVYPVA LSEKLRALVD SLSARGPL
Length:538
Mass (Da):58,265
Last modified:March 1, 2003 - v1
Checksum:iDFD3BC809E860A9E
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2R8VHP2A0A2R8VHP2_MOUSE
Nicotinate phosphoribosyltransferas...
Naprt
344Annotation score:

Sequence cautioni

The sequence BAC33575 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC33575 differs from that shown. Reason: Erroneous termination at position 517. Translated as Tyr.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti518P → T in BAC33575 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY214331 mRNA Translation: AAP69609.1
AK033661 mRNA Translation: BAC28414.1
AK049157 mRNA Translation: BAC33575.1 Sequence problems.
AK159127 mRNA Translation: BAE34841.1
AK170518 mRNA Translation: BAE41854.1
BC058800 mRNA Translation: AAH58800.1
CCDSiCCDS27552.1
RefSeqiNP_766195.2, NM_172607.3
UniGeneiMm.288368

Genome annotation databases

EnsembliENSMUST00000023237; ENSMUSP00000023237; ENSMUSG00000022574
GeneIDi223646
KEGGimmu:223646
UCSCiuc007whi.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY214331 mRNA Translation: AAP69609.1
AK033661 mRNA Translation: BAC28414.1
AK049157 mRNA Translation: BAC33575.1 Sequence problems.
AK159127 mRNA Translation: BAE34841.1
AK170518 mRNA Translation: BAE41854.1
BC058800 mRNA Translation: AAH58800.1
CCDSiCCDS27552.1
RefSeqiNP_766195.2, NM_172607.3
UniGeneiMm.288368

3D structure databases

ProteinModelPortaliQ8CC86
SMRiQ8CC86
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CC86, 3 interactors
MINTiQ8CC86
STRINGi10090.ENSMUSP00000023237

PTM databases

iPTMnetiQ8CC86
PhosphoSitePlusiQ8CC86
SwissPalmiQ8CC86

Proteomic databases

MaxQBiQ8CC86
PaxDbiQ8CC86
PeptideAtlasiQ8CC86
PRIDEiQ8CC86

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023237; ENSMUSP00000023237; ENSMUSG00000022574
GeneIDi223646
KEGGimmu:223646
UCSCiuc007whi.1 mouse

Organism-specific databases

CTDi93100
MGIiMGI:2442664 Naprt

Phylogenomic databases

eggNOGiKOG2511 Eukaryota
COG1488 LUCA
GeneTreeiENSGT00390000002664
HOGENOMiHOG000001111
HOVERGENiHBG062439
InParanoidiQ8CC86
KOiK00763
OMAiNPHIYKV
OrthoDBiEOG091G088G
PhylomeDBiQ8CC86
TreeFamiTF314732

Enzyme and pathway databases

UniPathwayi
UPA00253;UER00457

BRENDAi6.3.4.21 3474
ReactomeiR-MMU-197264 Nicotinamide salvaging
R-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

PROiPR:Q8CC86
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022574 Expressed in 155 organ(s), highest expression level in intestine
GenevisibleiQ8CC86 MM

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR007229 Nic_PRibTrfase-Fam
IPR006405 Nic_PRibTrfase_pncB
IPR036068 Nicotinate_pribotase_like_C
PANTHERiPTHR11098 PTHR11098, 1 hit
PIRSFiPIRSF000484 NAPRT, 1 hit
SUPFAMiSSF51690 SSF51690, 2 hits
TIGRFAMsiTIGR01513 NAPRTase_put, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPNCB_MOUSE
AccessioniPrimary (citable) accession number: Q8CC86
Secondary accession number(s): Q8C7W2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2003
Last modified: October 10, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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