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UniProtKB - Q8CBW3 (ABI1_MOUSE)

Protein

Abl interactor 1

Gene

Abi1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. In vitro, at least isoform 2 and isoform 4 suppress the transforming activity of Abelson murine leukemia virus (v-Abl) after overexpression in fibroblasts. May play a role in regulation EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.6 Publications

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Enzyme and pathway databases

ReactomeiR-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs

Names & Taxonomyi

Protein namesi
Recommended name:
Abl interactor 1
Alternative name(s):
Abelson interactor 1
Short name:
Abi-1
Ablphilin-1
Eps8 SH3 domain-binding protein
Short name:
Eps8-binding protein
Spectrin SH3 domain-binding protein 1
e3B1
Gene namesi
Name:Abi1Imported
Synonyms:Ssh3bp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:104913 Abi1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001917882 – 481Abl interactor 1Add BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei53PhosphotyrosineBy similarity1
Modified residuei174PhosphothreonineBy similarity1
Modified residuei178PhosphothreonineBy similarity1
Modified residuei183PhosphoserineCombined sources1
Modified residuei187PhosphoserineBy similarity1
Modified residuei213PhosphotyrosineCombined sources1
Modified residuei215PhosphothreonineCombined sources1
Modified residuei216PhosphoserineBy similarity1
Modified residuei222PhosphoserineBy similarity1
Modified residuei225PhosphoserineBy similarity1
Modified residuei292PhosphoserineBy similarity1
Modified residuei296PhosphoserineBy similarity1
Modified residuei428PhosphotyrosineCombined sources1
Modified residuei439PhosphoserineBy similarity1
Modified residuei480PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl. Seems to be phosphorylated at Tyr-53 by ABL1, required for nuclear but not for synaptic localization (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8CBW3
PRIDEiQ8CBW3

PTM databases

iPTMnetiQ8CBW3
PhosphoSitePlusiQ8CBW3

Expressioni

Tissue specificityi

Widely expressed with highest levels in bone marrow, spleen, brain, testes, and embryonic brain. In adult brain prominently expressed in the neocortex, hippocampus and dentate gyrus.2 Publications

Developmental stagei

Detected at E10 and E12 in developing brain, but does not appear more prominent in the neuroepithelium compared to the surrounding tissue.1 Publication

Gene expression databases

BgeeiENSMUSG00000058835 Expressed in 279 organ(s), highest expression level in CA1 field of hippocampus
CleanExiMM_ABI1
ExpressionAtlasiQ8CBW3 baseline and differential
GenevisibleiQ8CBW3 MM

Interactioni

Subunit structurei

Interacts with ENAH, Abelson murine leukemia virus V-ABL, ABL1, STX1A, SNAP25, VAMP2, and through its N-terminus with WASF1. Part of a complex consisting of ABI1, STX1A and SNAP25. Part of a complex consisting of ABI1, EPS8 and SOS1. Interacts with EPS8, SOS1, SOS2, GRB2, SPTA1, and the first SH3 domain of NCK1 (By similarity). Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 (NCKAP1l/HEM1 in hematopoietic cells) and WASF2/WAVE2. Interacts (via SH3 domain) with SHANK2 and SHANK3, but not SHANK1; the interaction is direct. Interacts with the heterodimer MYC:MAX; the interaction may enhance MYC:MAX transcriptional activity. Interacts with FNBP1L (via the SH3 domain), WASF2, and CDC42, but only in the presence of FNBP1L (By similarity).By similarity5 Publications

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi197905, 16 interactors
CORUMiQ8CBW3
DIPiDIP-29534N
IntActiQ8CBW3, 36 interactors
MINTiQ8CBW3
STRINGi10090.ENSMUSP00000118491

Structurei

3D structure databases

ProteinModelPortaliQ8CBW3
SMRiQ8CBW3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 107t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63
Domaini419 – 478SH3PROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni18 – 79Required for binding to WASF1Add BLAST62

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi337 – 391Pro-richAdd BLAST55

Domaini

The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A.

Sequence similaritiesi

Belongs to the ABI family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG2546 Eukaryota
ENOG410Y0MH LUCA
GeneTreeiENSGT00390000003756
HOGENOMiHOG000293213
HOVERGENiHBG050446
InParanoidiQ8CBW3
OMAiVGHGVKE
PhylomeDBiQ8CBW3
TreeFamiTF314303

Family and domain databases

CDDicd11971 SH3_Abi1, 1 hit
InterProiView protein in InterPro
IPR028457 ABI
IPR028456 ABI1
IPR035725 Abi1_SH3
IPR012849 Abl-interactor_HHR_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR000727 T_SNARE_dom
PANTHERiPTHR10460 PTHR10460, 1 hit
PTHR10460:SF2 PTHR10460:SF2, 1 hit
PfamiView protein in Pfam
PF07815 Abi_HHR, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit
PS50192 T_SNARE, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8CBW3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAELQMLLEE EIPSGKRALI ESYQNLTRVA DYCENNYIQA TDKRKALEET 
        60         70         80         90        100
KAYTTQSLAS VAYQINALAN NVLQLLDIQA SQLRRMESSI NHISQTVDIH 
       110        120        130        140        150
KEKVARREIG ILTTNKNTSR THKIIAPANM ERPVRYIRKP IDYTVLDDVG 
       160        170        180        190        200
HGVKWLKAKH GNNQPARTGT LSRTNPPTQK PPSPPVSGRG TLGRNTPYKT 
       210        220        230        240        250
LEPVKPPTVP NDYMTSPARL GSQHSPGRTA SLNQRPRTHS GSSGGSGSRE 
       260        270        280        290        300
NSGSSSIGIP IAVPTPSPPT AGPAAPGAAP GSQYGTMTRQ ISRHNSTTSS 
       310        320        330        340        350
TSSGGYRRTP SVAAQFSAQP HVNGGPLYSQ NSISVAPPPP PMPQLTPQIP 
       360        370        380        390        400
LTGFVARVQE NIADSPTPPP PPPPDDIPMF DDSPPPPPPP PVDYEDEEAA 
       410        420        430        440        450
VVQYSDPYAD GDPAWAPKNY IEKVVAIYDY TKDKDDELSF KEGAIIYVIK 
       460        470        480 
KNDDGWFEGV CNRVTGLFPG NYVESIMHYT D
Length:481
Mass (Da):52,288
Last modified:January 23, 2007 - v3
Checksum:i1654A1E89438BC1D
GO
Isoform 2 (identifier: Q8CBW3-2) [UniParc]FASTAAdd to basket
Also known as: short

The sequence of this isoform differs from the canonical sequence as follows:
     154-158: Missing.
     274-274: Missing.
     333-361: Missing.

Show »
Length:446
Mass (Da):48,512
Checksum:i805A2F6072F1D24B
GO
Isoform 3 (identifier: Q8CBW3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     154-158: Missing.

Show »
Length:476
Mass (Da):51,661
Checksum:iB7E38AA1E9ED7FA6
GO
Isoform 4 (identifier: Q8CBW3-4) [UniParc]FASTAAdd to basket
Also known as: long

The sequence of this isoform differs from the canonical sequence as follows:
     154-158: Missing.
     274-274: Missing.

Show »
Length:475
Mass (Da):51,590
Checksum:iA0A613AAD091B0F0
GO
Isoform 5 (identifier: Q8CBW3-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     154-158: Missing.
     274-362: AAPGAAPGSQ...GFVARVQENI → V

Show »
Length:388
Mass (Da):42,548
Checksum:i27FAB95E05BCA655
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7ZCU2B7ZCU2_MOUSE
Abl interactor 1
Abi1
393Annotation score:
B7ZCU0B7ZCU0_MOUSE
Abl interactor 1
Abi1
480Annotation score:
B7ZCU4B7ZCU4_MOUSE
Abl interactor 1
Abi1
451Annotation score:
B7ZCU3B7ZCU3_MOUSE
Abl interactor 1
Abi1
452Annotation score:
J3QNK8J3QNK8_MOUSE
Abl interactor 1
Abi1
481Annotation score:
B7ZCU5B7ZCU5_MOUSE
Abl interactor 1
Abi1
422Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti313A → T in AAH04657 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010756154 – 158Missing in isoform 2, isoform 3, isoform 4 and isoform 5. 5 Publications5
Alternative sequenceiVSP_022636274 – 362AAPGA…VQENI → V in isoform 5. 1 PublicationAdd BLAST89
Alternative sequenceiVSP_010757274Missing in isoform 2 and isoform 4. 3 Publications1
Alternative sequenceiVSP_010758333 – 361Missing in isoform 2. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF420251 mRNA Translation: AAL16036.1
AY033645 mRNA Translation: AAK59381.1
AK034476 mRNA Translation: BAC28722.1
AK152061 mRNA Translation: BAE30917.1
AK152184 mRNA Translation: BAE31015.1
AK151026 mRNA Translation: BAE30044.1
BC004657 mRNA Translation: AAH04657.1
U17698 mRNA Translation: AAB00373.1 Sequence problems.
CCDSiCCDS38058.1 [Q8CBW3-5]
CCDS38059.1 [Q8CBW3-3]
CCDS38060.1 [Q8CBW3-2]
CCDS38061.1 [Q8CBW3-1]
CCDS50513.1 [Q8CBW3-4]
RefSeqiNP_001070658.1, NM_001077190.3 [Q8CBW3-1]
NP_001070660.1, NM_001077192.3 [Q8CBW3-2]
NP_001070661.1, NM_001077193.3 [Q8CBW3-5]
NP_031406.2, NM_007380.4 [Q8CBW3-4]
UniGeneiMm.205647
Mm.249752

Genome annotation databases

EnsembliENSMUST00000114544; ENSMUSP00000110191; ENSMUSG00000058835 [Q8CBW3-5]
ENSMUST00000123948; ENSMUSP00000118491; ENSMUSG00000058835 [Q8CBW3-1]
ENSMUST00000126112; ENSMUSP00000117335; ENSMUSG00000058835 [Q8CBW3-3]
ENSMUST00000140164; ENSMUSP00000120462; ENSMUSG00000058835 [Q8CBW3-4]
ENSMUST00000149719; ENSMUSP00000120621; ENSMUSG00000058835 [Q8CBW3-2]
GeneIDi11308
KEGGimmu:11308
UCSCiuc008ins.2 mouse [Q8CBW3-1]
uc008inw.2 mouse [Q8CBW3-5]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF420251 mRNA Translation: AAL16036.1
AY033645 mRNA Translation: AAK59381.1
AK034476 mRNA Translation: BAC28722.1
AK152061 mRNA Translation: BAE30917.1
AK152184 mRNA Translation: BAE31015.1
AK151026 mRNA Translation: BAE30044.1
BC004657 mRNA Translation: AAH04657.1
U17698 mRNA Translation: AAB00373.1 Sequence problems.
CCDSiCCDS38058.1 [Q8CBW3-5]
CCDS38059.1 [Q8CBW3-3]
CCDS38060.1 [Q8CBW3-2]
CCDS38061.1 [Q8CBW3-1]
CCDS50513.1 [Q8CBW3-4]
RefSeqiNP_001070658.1, NM_001077190.3 [Q8CBW3-1]
NP_001070660.1, NM_001077192.3 [Q8CBW3-2]
NP_001070661.1, NM_001077193.3 [Q8CBW3-5]
NP_031406.2, NM_007380.4 [Q8CBW3-4]
UniGeneiMm.205647
Mm.249752

3D structure databases

ProteinModelPortaliQ8CBW3
SMRiQ8CBW3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197905, 16 interactors
CORUMiQ8CBW3
DIPiDIP-29534N
IntActiQ8CBW3, 36 interactors
MINTiQ8CBW3
STRINGi10090.ENSMUSP00000118491

PTM databases

iPTMnetiQ8CBW3
PhosphoSitePlusiQ8CBW3

Proteomic databases

PaxDbiQ8CBW3
PRIDEiQ8CBW3

Protocols and materials databases

DNASUi11308
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000114544; ENSMUSP00000110191; ENSMUSG00000058835 [Q8CBW3-5]
ENSMUST00000123948; ENSMUSP00000118491; ENSMUSG00000058835 [Q8CBW3-1]
ENSMUST00000126112; ENSMUSP00000117335; ENSMUSG00000058835 [Q8CBW3-3]
ENSMUST00000140164; ENSMUSP00000120462; ENSMUSG00000058835 [Q8CBW3-4]
ENSMUST00000149719; ENSMUSP00000120621; ENSMUSG00000058835 [Q8CBW3-2]
GeneIDi11308
KEGGimmu:11308
UCSCiuc008ins.2 mouse [Q8CBW3-1]
uc008inw.2 mouse [Q8CBW3-5]

Organism-specific databases

CTDi10006
MGIiMGI:104913 Abi1

Phylogenomic databases

eggNOGiKOG2546 Eukaryota
ENOG410Y0MH LUCA
GeneTreeiENSGT00390000003756
HOGENOMiHOG000293213
HOVERGENiHBG050446
InParanoidiQ8CBW3
OMAiVGHGVKE
PhylomeDBiQ8CBW3
TreeFamiTF314303

Enzyme and pathway databases

ReactomeiR-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs

Miscellaneous databases

ChiTaRSiAbi1 mouse
PROiPR:Q8CBW3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000058835 Expressed in 279 organ(s), highest expression level in CA1 field of hippocampus
CleanExiMM_ABI1
ExpressionAtlasiQ8CBW3 baseline and differential
GenevisibleiQ8CBW3 MM

Family and domain databases

CDDicd11971 SH3_Abi1, 1 hit
InterProiView protein in InterPro
IPR028457 ABI
IPR028456 ABI1
IPR035725 Abi1_SH3
IPR012849 Abl-interactor_HHR_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR000727 T_SNARE_dom
PANTHERiPTHR10460 PTHR10460, 1 hit
PTHR10460:SF2 PTHR10460:SF2, 1 hit
PfamiView protein in Pfam
PF07815 Abi_HHR, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit
PS50192 T_SNARE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiABI1_MOUSE
AccessioniPrimary (citable) accession number: Q8CBW3
Secondary accession number(s): Q3U8V0
, Q60747, Q91ZM5, Q923I9, Q99KH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 161 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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