UniProtKB - Q8CBQ5 (P4K2B_MOUSE)
Protein
Phosphatidylinositol 4-kinase type 2-beta
Gene
Pi4k2b
Organism
Mus musculus (Mouse)
Status
Functioni
Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking (By similarity).By similarity
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H+EC:2.7.1.67
Activity regulationi
Recruited and activated by membrane association. Binding to the microsomal membrane has been shown to enhance its activity (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 136 | ATPBy similarity | 1 | |
Binding sitei | 332 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 115 – 121 | ATPBy similarity | 7 | |
Nucleotide bindingi | 245 – 248 | ATPBy similarity | 4 |
GO - Molecular functioni
- 1-phosphatidylinositol 4-kinase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- endosome organization Source: GO_Central
- Golgi organization Source: GO_Central
- phosphatidylinositol phosphorylation Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1483248, Synthesis of PIPs at the ER membrane R-MMU-1660499, Synthesis of PIPs at the plasma membrane R-MMU-1660514, Synthesis of PIPs at the Golgi membrane R-MMU-1660516, Synthesis of PIPs at the early endosome membrane |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatidylinositol 4-kinase type 2-beta (EC:2.7.1.67)Alternative name(s): Phosphatidylinositol 4-kinase type II-beta |
Gene namesi | Name:Pi4k2b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914323, Pi4k2b |
Subcellular locationi
Other locations
Note: Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism (By similarity).By similarity
Endosome
- endosome Source: GO_Central
Golgi apparatus
- trans-Golgi network Source: GO_Central
Plasma Membrane
- plasma membrane Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000285165 | 1 – 469 | Phosphatidylinositol 4-kinase type 2-betaAdd BLAST | 469 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 37 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8CBQ5 |
PaxDbi | Q8CBQ5 |
PeptideAtlasi | Q8CBQ5 |
PRIDEi | Q8CBQ5 |
PTM databases
iPTMneti | Q8CBQ5 |
PhosphoSitePlusi | Q8CBQ5 |
SwissPalmi | Q8CBQ5 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029186, Expressed in left lung lobe and 277 other tissues |
Genevisiblei | Q8CBQ5, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000031081 |
Miscellaneous databases
RNActi | Q8CBQ5, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 117 – 424 | PI3K/PI4KAdd BLAST | 308 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 141 – 143 | Important for substrate bindingBy similarity | 3 | |
Regioni | 149 – 162 | Important for interaction with membranesBy similarityAdd BLAST | 14 | |
Regioni | 252 – 260 | Important for interaction with membranesBy similarity | 9 | |
Regioni | 345 – 354 | Important for interaction with membranesBy similarity | 10 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2381, Eukaryota |
GeneTreei | ENSGT00390000010434 |
HOGENOMi | CLU_032516_1_0_1 |
InParanoidi | Q8CBQ5 |
OMAi | ECGVFPE |
OrthoDBi | 1273723at2759 |
PhylomeDBi | Q8CBQ5 |
TreeFami | TF314740 |
Family and domain databases
InterProi | View protein in InterPro IPR039756, Lsb6/PI4K2 IPR000403, PI3/4_kinase_cat_dom |
PANTHERi | PTHR12865, PTHR12865, 1 hit |
Pfami | View protein in Pfam PF00454, PI3_PI4_kinase, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8CBQ5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAEACEPTRP SEDEDEEREP LLPRVAWAQP RRVAPGSAVR MQADEGADVL
60 70 80 90 100
REPATDEPPA VSGEGSISAS LSTELDRTRT TSSETNTFLE DPEFADIVLK
110 120 130 140 150
AEQAIEIGVF PERISQGSSG SYFVKDSKRN IIGVFKPKSE EPYGQLNPKW
160 170 180 190 200
TKYVHKVCCP CCFGRGCLLP NQGYLSEAGA YLVDVKLNLG IVPKTKVVWL
210 220 230 240 250
VSETFNYSAI DRAKSRGKKY ALEKVPKVGR KFHRIGLPPK VGSFQLFVKD
260 270 280 290 300
YKEAEYWLRR FEAEPLPENI RKQFQSQFEK LVILDYIIRN TDRGNDNWLV
310 320 330 340 350
KYDEMKYAKK IESEESNWID NKQLLIKIAA IDNGLAFPFK HPDEWRAYPF
360 370 380 390 400
HWAWLPQAKV PFSEETRNLI LPYISDMNFV QDLCEDLYEL FKTDKGFDRA
410 420 430 440 450
AFENQMSVMR GQILNLTQAL RDGKSPMQLA QMPCVIVECS KSGSQGRVVH
460
LGSSFTQTVH CRKPFFSSW
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JEK1 | A0A0G2JEK1_MOUSE | Phosphatidylinositol 4-kinase type ... | Pi4k2b | 89 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 199 | W → L in AAL04155 (Ref. 1) Curated | 1 | |
Sequence conflicti | 199 | W → L in BAB27819 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 229 – 236 | GRKFHRIG → AESPSDR in AAN37399 (Ref. 2) Curated | 8 | |
Sequence conflicti | 244 | F → S in AAN37399 (Ref. 2) Curated | 1 | |
Sequence conflicti | 244 | F → S in BAB30411 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 261 | F → Y in AAN37399 (Ref. 2) Curated | 1 | |
Sequence conflicti | 261 | F → Y in BAB30411 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 399 | R → K in AAH10257 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024828 | 84 – 129 | ETNTF…KDSKR → GPELCPFYSRSLCYKTTTAL GYY in isoform 2. 2 PublicationsAdd BLAST | 46 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF411321 mRNA Translation: AAL04155.1 AY148879 mRNA Translation: AAN37399.1 AK011751 mRNA Translation: BAB27819.1 AK016754 mRNA Translation: BAB30411.1 AK035523 mRNA Translation: BAC29091.1 AK170730 mRNA Translation: BAE41986.1 BC010257 mRNA Translation: AAH10257.1 BC062144 mRNA Translation: AAH62144.1 |
CCDSi | CCDS19287.1 [Q8CBQ5-1] CCDS39086.1 [Q8CBQ5-2] |
RefSeqi | NP_080227.2, NM_025951.3 [Q8CBQ5-1] NP_083020.2, NM_028744.3 [Q8CBQ5-2] |
Genome annotation databases
Ensembli | ENSMUST00000031081; ENSMUSP00000031081; ENSMUSG00000029186 [Q8CBQ5-1] ENSMUST00000031082; ENSMUSP00000031082; ENSMUSG00000029186 [Q8CBQ5-2] |
GeneIDi | 67073 |
KEGGi | mmu:67073 |
UCSCi | uc008xkq.2, mouse [Q8CBQ5-1] uc008xkr.2, mouse [Q8CBQ5-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF411321 mRNA Translation: AAL04155.1 AY148879 mRNA Translation: AAN37399.1 AK011751 mRNA Translation: BAB27819.1 AK016754 mRNA Translation: BAB30411.1 AK035523 mRNA Translation: BAC29091.1 AK170730 mRNA Translation: BAE41986.1 BC010257 mRNA Translation: AAH10257.1 BC062144 mRNA Translation: AAH62144.1 |
CCDSi | CCDS19287.1 [Q8CBQ5-1] CCDS39086.1 [Q8CBQ5-2] |
RefSeqi | NP_080227.2, NM_025951.3 [Q8CBQ5-1] NP_083020.2, NM_028744.3 [Q8CBQ5-2] |
3D structure databases
SMRi | Q8CBQ5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000031081 |
PTM databases
iPTMneti | Q8CBQ5 |
PhosphoSitePlusi | Q8CBQ5 |
SwissPalmi | Q8CBQ5 |
Proteomic databases
EPDi | Q8CBQ5 |
PaxDbi | Q8CBQ5 |
PeptideAtlasi | Q8CBQ5 |
PRIDEi | Q8CBQ5 |
Protocols and materials databases
Antibodypediai | 1318, 159 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000031081; ENSMUSP00000031081; ENSMUSG00000029186 [Q8CBQ5-1] ENSMUST00000031082; ENSMUSP00000031082; ENSMUSG00000029186 [Q8CBQ5-2] |
GeneIDi | 67073 |
KEGGi | mmu:67073 |
UCSCi | uc008xkq.2, mouse [Q8CBQ5-1] uc008xkr.2, mouse [Q8CBQ5-2] |
Organism-specific databases
CTDi | 55300 |
MGIi | MGI:1914323, Pi4k2b |
Phylogenomic databases
eggNOGi | KOG2381, Eukaryota |
GeneTreei | ENSGT00390000010434 |
HOGENOMi | CLU_032516_1_0_1 |
InParanoidi | Q8CBQ5 |
OMAi | ECGVFPE |
OrthoDBi | 1273723at2759 |
PhylomeDBi | Q8CBQ5 |
TreeFami | TF314740 |
Enzyme and pathway databases
Reactomei | R-MMU-1483248, Synthesis of PIPs at the ER membrane R-MMU-1660499, Synthesis of PIPs at the plasma membrane R-MMU-1660514, Synthesis of PIPs at the Golgi membrane R-MMU-1660516, Synthesis of PIPs at the early endosome membrane |
Miscellaneous databases
BioGRID-ORCSi | 67073, 0 hits in 18 CRISPR screens |
ChiTaRSi | Pi4k2b, mouse |
PROi | PR:Q8CBQ5 |
RNActi | Q8CBQ5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029186, Expressed in left lung lobe and 277 other tissues |
Genevisiblei | Q8CBQ5, MM |
Family and domain databases
InterProi | View protein in InterPro IPR039756, Lsb6/PI4K2 IPR000403, PI3/4_kinase_cat_dom |
PANTHERi | PTHR12865, PTHR12865, 1 hit |
Pfami | View protein in Pfam PF00454, PI3_PI4_kinase, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | P4K2B_MOUSE | |
Accessioni | Q8CBQ5Primary (citable) accession number: Q8CBQ5 Secondary accession number(s): Q91Z30, Q9D072, Q9D471 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families