UniProtKB - Q8CBQ5 (P4K2B_MOUSE)
Phosphatidylinositol 4-kinase type 2-beta
Pi4k2b
Functioni
Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking.
By similarityCatalytic activityi
- a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H+By similarityEC:2.7.1.67By similarityThis reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 121 | ATPBy similarity | 1 | |
Binding sitei | 136 | ATPBy similarity | 1 | |
Binding sitei | 332 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 245 – 248 | ATPBy similarity | 4 | |
Nucleotide bindingi | 259 – 260 | ATPBy similarity | 2 |
GO - Molecular functioni
- 1-phosphatidylinositol 4-kinase activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- endosome organization Source: GO_Central
- Golgi organization Source: GO_Central
- phosphatidylinositol phosphate biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Lipid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1483248, Synthesis of PIPs at the ER membrane R-MMU-1660499, Synthesis of PIPs at the plasma membrane R-MMU-1660514, Synthesis of PIPs at the Golgi membrane R-MMU-1660516, Synthesis of PIPs at the early endosome membrane |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatidylinositol 4-kinase type 2-beta (EC:2.7.1.67By similarity)Alternative name(s): Phosphatidylinositol 4-kinase type II-beta |
Gene namesi | Name:Pi4k2b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914323, Pi4k2b |
VEuPathDBi | HostDB:ENSMUSG00000029186 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Endosome
- Early endosome membrane By similarity
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity
Golgi apparatus
- Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity
Cytoplasm and Cytosol
- cytosol By similarity
Note: Mainly cytosolic, association with membranes of the Golgi, endoplasmic and plasma membrane is stimulated by active RAC1. Association with early endosomes has not been confirmed.By similarity
Cytosol
- cytosol Source: UniProtKB
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB
Endosome
- endosome Source: GO_Central
Golgi apparatus
- Golgi membrane Source: UniProtKB
- trans-Golgi network Source: GO_Central
Plasma Membrane
- plasma membrane Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Endoplasmic reticulum, Endosome, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000285165 | 1 – 469 | Phosphatidylinositol 4-kinase type 2-betaAdd BLAST | 469 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 37 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8CBQ5 |
MaxQBi | Q8CBQ5 |
PaxDbi | Q8CBQ5 |
PeptideAtlasi | Q8CBQ5 |
PRIDEi | Q8CBQ5 |
ProteomicsDBi | 294095 [Q8CBQ5-1] 294096 [Q8CBQ5-2] |
PTM databases
iPTMneti | Q8CBQ5 |
PhosphoSitePlusi | Q8CBQ5 |
SwissPalmi | Q8CBQ5 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029186, Expressed in left lung lobe and 286 other tissues |
Genevisiblei | Q8CBQ5, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000031081 |
Miscellaneous databases
RNActi | Q8CBQ5, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 108 – 439 | PI3K/PI4K catalyticPROSITE-ProRule annotationAdd BLAST | 332 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 84 | DisorderedSequence analysisAdd BLAST | 84 | |
Regioni | 114 – 120 | G-loopPROSITE-ProRule annotation | 7 | |
Regioni | 141 – 143 | Important for substrate bindingBy similarity | 3 | |
Regioni | 149 – 162 | Important for interaction with membranesBy similarityAdd BLAST | 14 | |
Regioni | 252 – 260 | Important for interaction with membranesBy similarity | 9 | |
Regioni | 289 – 297 | Catalytic loopPROSITE-ProRule annotation | 9 | |
Regioni | 330 – 350 | Activation loopPROSITE-ProRule annotationAdd BLAST | 21 | |
Regioni | 345 – 354 | Important for interaction with membranesBy similarity | 10 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 65 – 84 | Polar residuesSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2381, Eukaryota |
GeneTreei | ENSGT00390000010434 |
HOGENOMi | CLU_032516_1_0_1 |
InParanoidi | Q8CBQ5 |
OMAi | FHWAWLS |
OrthoDBi | 1273723at2759 |
PhylomeDBi | Q8CBQ5 |
TreeFami | TF314740 |
Family and domain databases
InterProi | View protein in InterPro IPR039756, Lsb6/PI4K2 IPR000403, PI3/4_kinase_cat_dom |
PANTHERi | PTHR12865, PTHR12865, 1 hit |
Pfami | View protein in Pfam PF00454, PI3_PI4_kinase, 1 hit |
PROSITEi | View protein in PROSITE PS50290, PI3_4_KINASE_3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAEACEPTRP SEDEDEEREP LLPRVAWAQP RRVAPGSAVR MQADEGADVL
60 70 80 90 100
REPATDEPPA VSGEGSISAS LSTELDRTRT TSSETNTFLE DPEFADIVLK
110 120 130 140 150
AEQAIEIGVF PERISQGSSG SYFVKDSKRN IIGVFKPKSE EPYGQLNPKW
160 170 180 190 200
TKYVHKVCCP CCFGRGCLLP NQGYLSEAGA YLVDVKLNLG IVPKTKVVWL
210 220 230 240 250
VSETFNYSAI DRAKSRGKKY ALEKVPKVGR KFHRIGLPPK VGSFQLFVKD
260 270 280 290 300
YKEAEYWLRR FEAEPLPENI RKQFQSQFEK LVILDYIIRN TDRGNDNWLV
310 320 330 340 350
KYDEMKYAKK IESEESNWID NKQLLIKIAA IDNGLAFPFK HPDEWRAYPF
360 370 380 390 400
HWAWLPQAKV PFSEETRNLI LPYISDMNFV QDLCEDLYEL FKTDKGFDRA
410 420 430 440 450
AFENQMSVMR GQILNLTQAL RDGKSPMQLA QMPCVIVECS KSGSQGRVVH
460
LGSSFTQTVH CRKPFFSSW
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JEK1 | A0A0G2JEK1_MOUSE | Phosphatidylinositol 4-kinase type ... | Pi4k2b | 89 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 199 | W → L in AAL04155 (Ref. 1) Curated | 1 | |
Sequence conflicti | 199 | W → L in BAB27819 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 229 – 236 | GRKFHRIG → AESPSDR in AAN37399 (Ref. 2) Curated | 8 | |
Sequence conflicti | 244 | F → S in AAN37399 (Ref. 2) Curated | 1 | |
Sequence conflicti | 244 | F → S in BAB30411 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 261 | F → Y in AAN37399 (Ref. 2) Curated | 1 | |
Sequence conflicti | 261 | F → Y in BAB30411 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 399 | R → K in AAH10257 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024828 | 84 – 129 | ETNTF…KDSKR → GPELCPFYSRSLCYKTTTAL GYY in isoform 2. 2 PublicationsAdd BLAST | 46 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF411321 mRNA Translation: AAL04155.1 AY148879 mRNA Translation: AAN37399.1 AK011751 mRNA Translation: BAB27819.1 AK016754 mRNA Translation: BAB30411.1 AK035523 mRNA Translation: BAC29091.1 AK170730 mRNA Translation: BAE41986.1 BC010257 mRNA Translation: AAH10257.1 BC062144 mRNA Translation: AAH62144.1 |
CCDSi | CCDS19287.1 [Q8CBQ5-1] CCDS39086.1 [Q8CBQ5-2] |
RefSeqi | NP_080227.2, NM_025951.3 [Q8CBQ5-1] NP_083020.2, NM_028744.3 [Q8CBQ5-2] |
Genome annotation databases
Ensembli | ENSMUST00000031081; ENSMUSP00000031081; ENSMUSG00000029186 [Q8CBQ5-1] ENSMUST00000031082; ENSMUSP00000031082; ENSMUSG00000029186 [Q8CBQ5-2] |
GeneIDi | 67073 |
KEGGi | mmu:67073 |
UCSCi | uc008xkq.2, mouse [Q8CBQ5-1] uc008xkr.2, mouse [Q8CBQ5-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF411321 mRNA Translation: AAL04155.1 AY148879 mRNA Translation: AAN37399.1 AK011751 mRNA Translation: BAB27819.1 AK016754 mRNA Translation: BAB30411.1 AK035523 mRNA Translation: BAC29091.1 AK170730 mRNA Translation: BAE41986.1 BC010257 mRNA Translation: AAH10257.1 BC062144 mRNA Translation: AAH62144.1 |
CCDSi | CCDS19287.1 [Q8CBQ5-1] CCDS39086.1 [Q8CBQ5-2] |
RefSeqi | NP_080227.2, NM_025951.3 [Q8CBQ5-1] NP_083020.2, NM_028744.3 [Q8CBQ5-2] |
3D structure databases
AlphaFoldDBi | Q8CBQ5 |
SMRi | Q8CBQ5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000031081 |
PTM databases
iPTMneti | Q8CBQ5 |
PhosphoSitePlusi | Q8CBQ5 |
SwissPalmi | Q8CBQ5 |
Proteomic databases
EPDi | Q8CBQ5 |
MaxQBi | Q8CBQ5 |
PaxDbi | Q8CBQ5 |
PeptideAtlasi | Q8CBQ5 |
PRIDEi | Q8CBQ5 |
ProteomicsDBi | 294095 [Q8CBQ5-1] 294096 [Q8CBQ5-2] |
Protocols and materials databases
Antibodypediai | 1318, 185 antibodies from 26 providers |
DNASUi | 67073 |
Genome annotation databases
Ensembli | ENSMUST00000031081; ENSMUSP00000031081; ENSMUSG00000029186 [Q8CBQ5-1] ENSMUST00000031082; ENSMUSP00000031082; ENSMUSG00000029186 [Q8CBQ5-2] |
GeneIDi | 67073 |
KEGGi | mmu:67073 |
UCSCi | uc008xkq.2, mouse [Q8CBQ5-1] uc008xkr.2, mouse [Q8CBQ5-2] |
Organism-specific databases
CTDi | 55300 |
MGIi | MGI:1914323, Pi4k2b |
VEuPathDBi | HostDB:ENSMUSG00000029186 |
Phylogenomic databases
eggNOGi | KOG2381, Eukaryota |
GeneTreei | ENSGT00390000010434 |
HOGENOMi | CLU_032516_1_0_1 |
InParanoidi | Q8CBQ5 |
OMAi | FHWAWLS |
OrthoDBi | 1273723at2759 |
PhylomeDBi | Q8CBQ5 |
TreeFami | TF314740 |
Enzyme and pathway databases
Reactomei | R-MMU-1483248, Synthesis of PIPs at the ER membrane R-MMU-1660499, Synthesis of PIPs at the plasma membrane R-MMU-1660514, Synthesis of PIPs at the Golgi membrane R-MMU-1660516, Synthesis of PIPs at the early endosome membrane |
Miscellaneous databases
BioGRID-ORCSi | 67073, 2 hits in 74 CRISPR screens |
ChiTaRSi | Pi4k2b, mouse |
PROi | PR:Q8CBQ5 |
RNActi | Q8CBQ5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029186, Expressed in left lung lobe and 286 other tissues |
Genevisiblei | Q8CBQ5, MM |
Family and domain databases
InterProi | View protein in InterPro IPR039756, Lsb6/PI4K2 IPR000403, PI3/4_kinase_cat_dom |
PANTHERi | PTHR12865, PTHR12865, 1 hit |
Pfami | View protein in Pfam PF00454, PI3_PI4_kinase, 1 hit |
PROSITEi | View protein in PROSITE PS50290, PI3_4_KINASE_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | P4K2B_MOUSE | |
Accessioni | Q8CBQ5Primary (citable) accession number: Q8CBQ5 Secondary accession number(s): Q91Z30, Q9D072, Q9D471 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families