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Protein

Radical S-adenosyl methionine domain-containing protein 2

Gene

Rsad2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interferon-inducible iron-sulfur (4FE-4S) cluster-binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of viruses, including west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus and vesicular stomatitis virus (VSV). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphosphate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T-helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins.4 Publications

Cofactori

[4Fe-4S] clusterCombined sources1 PublicationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.Combined sources1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi84Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources1 Publication1
Metal bindingi88Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources1 Publication1
Metal bindingi91Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAntiviral defense, Immunity, Innate immunity
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Radical S-adenosyl methionine domain-containing protein 2
Alternative name(s):
Viperin
Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible
Gene namesi
Name:Rsad2Imported
Synonyms:Vig1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1929628 Rsad2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003095841 – 362Radical S-adenosyl methionine domain-containing protein 2Add BLAST362

Proteomic databases

MaxQBiQ8CBB9
PaxDbiQ8CBB9
PRIDEiQ8CBB9

PTM databases

iPTMnetiQ8CBB9
PhosphoSitePlusiQ8CBB9

Expressioni

Tissue specificityi

Expressed at higher levels in atherosclerotic arteries than in normal arteries.1 Publication

Inductioni

By interferon type I, type II and LPS. Induced by infection with Vesicular stomatitis virus and pseudorabies virus in dendritic cells, presumably through type I interferon pathway.2 Publications

Gene expression databases

BgeeiENSMUSG00000020641 Expressed in 192 organ(s), highest expression level in blood
CleanExiMM_RSAD2
ExpressionAtlasiQ8CBB9 baseline and differential
GenevisibleiQ8CBB9 MM

Interactioni

Subunit structurei

Homodimer. Interacts with FPPS (By similarity). Interacts (via C-terminus) with VAPA/VAP33 (via C-terminus) and inhibits its interaction with hepatitis virus C (HCV) protein NS5A. Interacts with HADHB (By similarity). Interacts with IRAK1 and TRAF6.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi208378, 5 interactors
STRINGi10090.ENSMUSP00000020970

Structurei

Secondary structure

1362
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8CBB9
SMRiQ8CBB9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region (1-43) is necessary for its localization to the endoplasmic reticulum membrane and lipid droplet.

Sequence similaritiesi

Belongs to the radical SAM superfamily. RSAD2 family.Curated

Phylogenomic databases

eggNOGiENOG410IGZE Eukaryota
ENOG410XQM5 LUCA
GeneTreeiENSGT00390000013670
HOVERGENiHBG053099
InParanoidiQ8CBB9
KOiK15045
OMAiINRFNVE
OrthoDBiEOG091G0GXY
PhylomeDBiQ8CBB9
TreeFamiTF300085

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
IPR034395 SPASM/twitch_domain-containing
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
SFLDiSFLDG01067 SPASM/twitch_domain_containing, 1 hit
SFLDS00029 Radical_SAM, 2 hits
SMARTiView protein in SMART
SM00729 Elp3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8CBB9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGMLVPTALA ARLLSLFQQQ LGSLWSGLAI LFCWLRIALG WLDPGKEQPQ
60 70 80 90 100
VRGEPEDTQE TQEDGNSTQP TTPVSVNYHF TRQCNYKCGF CFHTAKTSFV
110 120 130 140 150
LPLEEAKRGL LLLKQAGLEK INFSGGEPFL QDRGEYLGKL VRFCKEELAL
160 170 180 190 200
PSVSIVSNGS LIRERWFKDY GEYLDILAIS CDSFDEQVNA LIGRGQGKKN
210 220 230 240 250
HVENLQKLRR WCRDYKVAFK INSVINRFNV DEDMNEHIKA LSPVRWKVFQ
260 270 280 290 300
CLLIEGENSG EDALREAERF LISNEEFETF LERHKEVSCL VPESNQKMKD
310 320 330 340 350
SYLILDEYMR FLNCTGGRKD PSKSILDVGV EEAIKFSGFD EKMFLKRGGK
360
YVWSKADLKL DW
Length:362
Mass (Da):41,524
Last modified:March 1, 2003 - v1
Checksum:iF56F0BBEC9F20E50
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RJ49D6RJ49_MOUSE
Radical S-adenosyl methionine domai...
Rsad2
187Annotation score:

Sequence cautioni

The sequence BAE29281 differs from that shown. Reason: Erroneous termination at position 46. Translated as Lys.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15S → R in BAE31578 (PubMed:16141072).Curated1
Sequence conflicti15S → R in BAE30379 (PubMed:16141072).Curated1
Sequence conflicti15S → R in BAE29807 (PubMed:16141072).Curated1
Sequence conflicti15S → R in BAE29764 (PubMed:16141072).Curated1
Sequence conflicti26S → C in BAE30676 (PubMed:16141072).Curated1
Sequence conflicti26S → G in BAE31898 (PubMed:16141072).Curated1
Sequence conflicti26S → G in BAE31903 (PubMed:16141072).Curated1
Sequence conflicti52R → P in BAE30920 (PubMed:16141072).Curated1
Sequence conflicti55P → L in AAF60314 (PubMed:11038379).Curated1
Sequence conflicti55P → L in CAJ18585 (Ref. 3) Curated1
Sequence conflicti55P → L in AAH57868 (PubMed:15489334).Curated1
Sequence conflicti55P → L in AAL50054 (PubMed:11752458).Curated1
Sequence conflicti57D → E in AAF60314 (PubMed:11038379).Curated1
Sequence conflicti57D → E in CAJ18585 (Ref. 3) Curated1
Sequence conflicti57D → E in AAH57868 (PubMed:15489334).Curated1
Sequence conflicti57D → E in AAL50054 (PubMed:11752458).Curated1
Sequence conflicti70P → R in AAF60314 (PubMed:11038379).Curated1
Sequence conflicti70P → R in CAJ18585 (Ref. 3) Curated1
Sequence conflicti70P → R in AAH57868 (PubMed:15489334).Curated1
Sequence conflicti70P → R in AAL50054 (PubMed:11752458).Curated1
Sequence conflicti73P → T in BAE31898 (PubMed:16141072).Curated1
Sequence conflicti94T → K in BAE30826 (PubMed:16141072).Curated1
Sequence conflicti94T → K in BAE32092 (PubMed:16141072).Curated1
Sequence conflicti125G → E in BAE29848 (PubMed:16141072).Curated1
Sequence conflicti148L → Q in BAE31064 (PubMed:16141072).Curated1
Sequence conflicti149A → S in BAE30826 (PubMed:16141072).Curated1
Sequence conflicti163R → Q in AAF60314 (PubMed:11038379).Curated1
Sequence conflicti163R → Q in AAL50054 (PubMed:11752458).Curated1
Sequence conflicti211W → R in BAE31565 (PubMed:16141072).Curated1
Sequence conflicti211W → R in BAE31383 (PubMed:16141072).Curated1
Sequence conflicti211W → R in BAE30737 (PubMed:16141072).Curated1
Sequence conflicti241L → Q in BAE31898 (PubMed:16141072).Curated1
Sequence conflicti241L → Q in BAE31903 (PubMed:16141072).Curated1
Sequence conflicti247K → E in BAE30673 (PubMed:16141072).Curated1
Sequence conflicti247K → E in BAE30203 (PubMed:16141072).Curated1
Sequence conflicti262D → G in BAE30726 (PubMed:16141072).Curated1
Sequence conflicti270F → L in BAE31348 (PubMed:16141072).Curated1
Sequence conflicti273S → G in BAE31064 (PubMed:16141072).Curated1
Sequence conflicti339F → Y in BAE30676 (PubMed:16141072).Curated1
Sequence conflicti339F → Y in BAE30787 (PubMed:16141072).Curated1
Sequence conflicti339F → Y in BAE30790 (PubMed:16141072).Curated1
Sequence conflicti339F → Y in BAE30920 (PubMed:16141072).Curated1
Sequence conflicti339F → Y in BAE30927 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF236064 mRNA Translation: AAF60314.2
AK036373 mRNA Translation: BAC29401.1
AK150069 mRNA Translation: BAE29281.1 Sequence problems.
AK150406 mRNA Translation: BAE29532.1
AK150425 mRNA Translation: BAE29548.1
AK150525 mRNA Translation: BAE29636.1
AK150616 mRNA Translation: BAE29706.1
AK150684 mRNA Translation: BAE29764.1
AK150731 mRNA Translation: BAE29807.1
AK150767 mRNA Translation: BAE29833.1
AK150783 mRNA Translation: BAE29848.1
AK150788 mRNA Translation: BAE29852.1
AK150812 mRNA Translation: BAE29875.1
AK150835 mRNA Translation: BAE29895.1
AK151140 mRNA Translation: BAE30147.1
AK151185 mRNA Translation: BAE30184.1
AK151191 mRNA Translation: BAE30188.1
AK151206 mRNA Translation: BAE30203.1
AK151286 mRNA Translation: BAE30271.1
AK151308 mRNA Translation: BAE30290.1
AK151339 mRNA Translation: BAE30317.1
AK151389 mRNA Translation: BAE30360.1
AK151410 mRNA Translation: BAE30375.1
AK151412 mRNA Translation: BAE30377.1
AK151414 mRNA Translation: BAE30379.1
AK151476 mRNA Translation: BAE30431.1
AK151502 mRNA Translation: BAE30453.1
AK151538 mRNA Translation: BAE30485.1
AK151767 mRNA Translation: BAE30673.1
AK151770 mRNA Translation: BAE30676.1
AK151825 mRNA Translation: BAE30722.1
AK151833 mRNA Translation: BAE30726.1
AK151846 mRNA Translation: BAE30737.1
AK151906 mRNA Translation: BAE30787.1
AK151909 mRNA Translation: BAE30790.1
AK151940 mRNA Translation: BAE30816.1
AK151954 mRNA Translation: BAE30826.1
AK151971 mRNA Translation: BAE30840.1
AK152050 mRNA Translation: BAE30906.1
AK152065 mRNA Translation: BAE30920.1
AK152075 mRNA Translation: BAE30927.1
AK152235 mRNA Translation: BAE31060.1
AK152239 mRNA Translation: BAE31062.1
AK152241 mRNA Translation: BAE31064.1
AK152310 mRNA Translation: BAE31116.1
AK152354 mRNA Translation: BAE31146.1
AK152493 mRNA Translation: BAE31264.1
AK152600 mRNA Translation: BAE31348.1
AK152601 mRNA Translation: BAE31349.1
AK152631 mRNA Translation: BAE31374.1
AK152644 mRNA Translation: BAE31383.1
AK152664 mRNA Translation: BAE31399.1
AK152796 mRNA Translation: BAE31501.1
AK152861 mRNA Translation: BAE31552.1
AK152880 mRNA Translation: BAE31565.1
AK152895 mRNA Translation: BAE31578.1
AK152934 mRNA Translation: BAE31608.1
AK152962 mRNA Translation: BAE31625.1
AK153003 mRNA Translation: BAE31643.1
AK153024 mRNA Translation: BAE31658.1
AK153062 mRNA Translation: BAE31688.1
AK153077 mRNA Translation: BAE31702.1
AK153078 mRNA Translation: BAE31703.1
AK153232 mRNA Translation: BAE31825.1
AK153234 mRNA Translation: BAE31827.1
AK153306 mRNA Translation: BAE31887.1
AK153318 mRNA Translation: BAE31898.1
AK153324 mRNA Translation: BAE31903.1
AK153435 mRNA Translation: BAE31992.1
AK153556 mRNA Translation: BAE32092.1
AK159147 mRNA Translation: BAE34854.1
CT010378 mRNA Translation: CAJ18585.1
BC057868 mRNA Translation: AAH57868.1
AF442152 mRNA Translation: AAL50054.1
CCDSiCCDS25848.1
RefSeqiNP_067359.2, NM_021384.4
UniGeneiMm.24045

Genome annotation databases

EnsembliENSMUST00000020970; ENSMUSP00000020970; ENSMUSG00000020641
GeneIDi58185
KEGGimmu:58185
UCSCiuc007nfh.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF236064 mRNA Translation: AAF60314.2
AK036373 mRNA Translation: BAC29401.1
AK150069 mRNA Translation: BAE29281.1 Sequence problems.
AK150406 mRNA Translation: BAE29532.1
AK150425 mRNA Translation: BAE29548.1
AK150525 mRNA Translation: BAE29636.1
AK150616 mRNA Translation: BAE29706.1
AK150684 mRNA Translation: BAE29764.1
AK150731 mRNA Translation: BAE29807.1
AK150767 mRNA Translation: BAE29833.1
AK150783 mRNA Translation: BAE29848.1
AK150788 mRNA Translation: BAE29852.1
AK150812 mRNA Translation: BAE29875.1
AK150835 mRNA Translation: BAE29895.1
AK151140 mRNA Translation: BAE30147.1
AK151185 mRNA Translation: BAE30184.1
AK151191 mRNA Translation: BAE30188.1
AK151206 mRNA Translation: BAE30203.1
AK151286 mRNA Translation: BAE30271.1
AK151308 mRNA Translation: BAE30290.1
AK151339 mRNA Translation: BAE30317.1
AK151389 mRNA Translation: BAE30360.1
AK151410 mRNA Translation: BAE30375.1
AK151412 mRNA Translation: BAE30377.1
AK151414 mRNA Translation: BAE30379.1
AK151476 mRNA Translation: BAE30431.1
AK151502 mRNA Translation: BAE30453.1
AK151538 mRNA Translation: BAE30485.1
AK151767 mRNA Translation: BAE30673.1
AK151770 mRNA Translation: BAE30676.1
AK151825 mRNA Translation: BAE30722.1
AK151833 mRNA Translation: BAE30726.1
AK151846 mRNA Translation: BAE30737.1
AK151906 mRNA Translation: BAE30787.1
AK151909 mRNA Translation: BAE30790.1
AK151940 mRNA Translation: BAE30816.1
AK151954 mRNA Translation: BAE30826.1
AK151971 mRNA Translation: BAE30840.1
AK152050 mRNA Translation: BAE30906.1
AK152065 mRNA Translation: BAE30920.1
AK152075 mRNA Translation: BAE30927.1
AK152235 mRNA Translation: BAE31060.1
AK152239 mRNA Translation: BAE31062.1
AK152241 mRNA Translation: BAE31064.1
AK152310 mRNA Translation: BAE31116.1
AK152354 mRNA Translation: BAE31146.1
AK152493 mRNA Translation: BAE31264.1
AK152600 mRNA Translation: BAE31348.1
AK152601 mRNA Translation: BAE31349.1
AK152631 mRNA Translation: BAE31374.1
AK152644 mRNA Translation: BAE31383.1
AK152664 mRNA Translation: BAE31399.1
AK152796 mRNA Translation: BAE31501.1
AK152861 mRNA Translation: BAE31552.1
AK152880 mRNA Translation: BAE31565.1
AK152895 mRNA Translation: BAE31578.1
AK152934 mRNA Translation: BAE31608.1
AK152962 mRNA Translation: BAE31625.1
AK153003 mRNA Translation: BAE31643.1
AK153024 mRNA Translation: BAE31658.1
AK153062 mRNA Translation: BAE31688.1
AK153077 mRNA Translation: BAE31702.1
AK153078 mRNA Translation: BAE31703.1
AK153232 mRNA Translation: BAE31825.1
AK153234 mRNA Translation: BAE31827.1
AK153306 mRNA Translation: BAE31887.1
AK153318 mRNA Translation: BAE31898.1
AK153324 mRNA Translation: BAE31903.1
AK153435 mRNA Translation: BAE31992.1
AK153556 mRNA Translation: BAE32092.1
AK159147 mRNA Translation: BAE34854.1
CT010378 mRNA Translation: CAJ18585.1
BC057868 mRNA Translation: AAH57868.1
AF442152 mRNA Translation: AAL50054.1
CCDSiCCDS25848.1
RefSeqiNP_067359.2, NM_021384.4
UniGeneiMm.24045

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5VSLX-ray1.97A/B45-362[»]
5VSMX-ray1.70A/B45-362[»]
ProteinModelPortaliQ8CBB9
SMRiQ8CBB9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208378, 5 interactors
STRINGi10090.ENSMUSP00000020970

PTM databases

iPTMnetiQ8CBB9
PhosphoSitePlusiQ8CBB9

Proteomic databases

MaxQBiQ8CBB9
PaxDbiQ8CBB9
PRIDEiQ8CBB9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020970; ENSMUSP00000020970; ENSMUSG00000020641
GeneIDi58185
KEGGimmu:58185
UCSCiuc007nfh.2 mouse

Organism-specific databases

CTDi91543
MGIiMGI:1929628 Rsad2

Phylogenomic databases

eggNOGiENOG410IGZE Eukaryota
ENOG410XQM5 LUCA
GeneTreeiENSGT00390000013670
HOVERGENiHBG053099
InParanoidiQ8CBB9
KOiK15045
OMAiINRFNVE
OrthoDBiEOG091G0GXY
PhylomeDBiQ8CBB9
TreeFamiTF300085

Miscellaneous databases

PROiPR:Q8CBB9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020641 Expressed in 192 organ(s), highest expression level in blood
CleanExiMM_RSAD2
ExpressionAtlasiQ8CBB9 baseline and differential
GenevisibleiQ8CBB9 MM

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
IPR034395 SPASM/twitch_domain-containing
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
SFLDiSFLDG01067 SPASM/twitch_domain_containing, 1 hit
SFLDS00029 Radical_SAM, 2 hits
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRSAD2_MOUSE
AccessioniPrimary (citable) accession number: Q8CBB9
Secondary accession number(s): Q3U5I6
, Q3U5T4, Q3U622, Q3U627, Q3U7I6, Q3U7M1, Q3U8F4, Q3U8U7, Q3U941, Q3U977, Q3U9E1, Q3U9J0, Q3U9J3, Q3UBW6, Q3UC07, Q3UDI0, Q6PEU4, Q8VHM2, Q9JHD4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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