UniProtKB - Q8C9X6 (EPC1_MOUSE)
Enhancer of polycomb homolog 1
Epc1
Functioni
Component of the NuA4 histone acetyltransferase (HAT) complex, a multiprotein complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A (PubMed:28694333).
The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) by promoting homologous recombination (HR) (By similarity).
The NuA4 complex is also required for spermatid development by promoting acetylation of histones: histone acetylation is required for histone replacement during the transition from round to elongating spermatids (PubMed:28694333).
In the NuA4 complex, EPC1 is required to recruit MBTD1 into the complex (By similarity).
By similarity1 PublicationGO - Molecular functioni
- chromatin-protein adaptor Source: UniProtKB
GO - Biological processi
- double-strand break repair via homologous recombination Source: UniProtKB
- histone acetylation Source: MGI
- histone H2A acetylation Source: UniProtKB
- histone H4 acetylation Source: UniProtKB
- negative regulation of gene expression, epigenetic Source: MGI
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of double-strand break repair via homologous recombination Source: MGI
- positive regulation of striated muscle cell differentiation Source: MGI
- positive regulation of transcription, DNA-templated Source: ComplexPortal
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of apoptotic process Source: ComplexPortal
- regulation of cell cycle Source: MGI
- regulation of chromatin organization Source: ComplexPortal
- regulation of double-strand break repair Source: ComplexPortal
- regulation of growth Source: UniProtKB-KW
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, Chromatin regulator |
Biological process | Differentiation, Growth regulation, Spermatogenesis, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-8953750, Transcriptional Regulation by E2F6 |
Names & Taxonomyi
Protein namesi | Recommended name: Enhancer of polycomb homolog 1Curated |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1278322, Epc1 |
VEuPathDBi | HostDB:ENSMUSG00000024240 |
Subcellular locationi
Nucleus
- NuA4 histone acetyltransferase complex Source: UniProtKB
- nuclear membrane Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
- Piccolo NuA4 histone acetyltransferase complex Source: UniProtKB
Other locations
- intracellular membrane-bounded organelle Source: MGI
- nucleosome Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000214154 | 1 – 813 | Enhancer of polycomb homolog 1Add BLAST | 813 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 319 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 538 | PhosphoserineBy similarity | 1 | |
Cross-linki | 650 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8C9X6 |
jPOSTi | Q8C9X6 |
MaxQBi | Q8C9X6 |
PaxDbi | Q8C9X6 |
PRIDEi | Q8C9X6 |
ProteomicsDBi | 275930 [Q8C9X6-1] 275931 [Q8C9X6-2] |
PTM databases
iPTMneti | Q8C9X6 |
PhosphoSitePlusi | Q8C9X6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000024240, Expressed in pituitary gland and 307 other tissues |
ExpressionAtlasi | Q8C9X6, baseline and differential |
Genevisiblei | Q8C9X6, MM |
Interactioni
Subunit structurei
Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6 (By similarity). KAT5/TIP60, EPC1, and ING3 together constitute a minimal HAT complex termed Piccolo NuA4 (By similarity).
Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41 (By similarity).
Interacts with TRIM27 (By similarity).
Interacts with MBTD1; interaction is direct and promotes recruitment of MBTD1 into the NuA4 histone acetyltransferase complex (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 199466, 11 interactors |
ComplexPortali | CPX-747, Piccolo NuA4 histone acetyltransferase complex CPX-990, NuA4 histone acetyltransferase complex |
IntActi | Q8C9X6, 5 interactors |
MINTi | Q8C9X6 |
STRINGi | 10090.ENSMUSP00000028100 |
Miscellaneous databases
RNActi | Q8C9X6, protein |
Structurei
3D structure databases
AlphaFoldDBi | Q8C9X6 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 310 – 403 | DisorderedSequence analysisAdd BLAST | 94 | |
Regioni | 484 – 513 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 528 – 577 | DisorderedSequence analysisAdd BLAST | 50 | |
Regioni | 779 – 813 | DisorderedSequence analysisAdd BLAST | 35 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 313 – 343 | Basic and acidic residuesSequence analysisAdd BLAST | 31 | |
Compositional biasi | 369 – 390 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 487 – 513 | Polar residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 560 – 577 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 799 – 813 | Polar residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2261, Eukaryota |
GeneTreei | ENSGT00940000155003 |
HOGENOMi | CLU_012781_0_0_1 |
InParanoidi | Q8C9X6 |
OMAi | XENHESE |
OrthoDBi | 957492at2759 |
PhylomeDBi | Q8C9X6 |
TreeFami | TF106438 |
Family and domain databases
InterProi | View protein in InterPro IPR024943, Enhancer_polycomb IPR019542, Enhancer_polycomb-like_N IPR009607, Enhancer_polycomb_C |
PANTHERi | PTHR14898, PTHR14898, 1 hit |
Pfami | View protein in Pfam PF06752, E_Pc_C, 1 hit PF10513, EPL1, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSKLSFRARA LDASKPLPVF RCEDLPDLHE YASINRAVPQ MPTGMEKEEE
60 70 80 90 100
SEHHLQRAIS AQQVYGEKRD NMVIPVPEAE SNIAYYESIY PGEFRMPKQL
110 120 130 140 150
IHIQPFSLDA EQPDYDLDSE DEVFVNKLKK KMDICPLQFE EMIDRLEKGS
160 170 180 190 200
GQQPVSLQEA KLLLKEDDEL IREVYEYWIK KRKTCRGSSL IPLVKQEKRD
210 220 230 240 250
GSSTNDPYVA FRRRTEKMQT RKNRKNDEAS YEKMLKLRRD LSRAVTILEM
260 270 280 290 300
IKRREKSKRE LLHLTLEIME KRYNLGDYSG EIMSEVMAQR QPVKPTYAIP
310 320 330 340 350
IIPITNSSQF KHQDATDSKE FKVNKQDKAD LIRPKRKYEK KPKVLPPSAA
360 370 380 390 400
APQQQSPAAL PGFSAKDLNQ YDFPSSDEEP LSQVLSGSSE AEEENDPDGP
410 420 430 440 450
FAFRRKAGCQ YYAPHLDQTG NWPWTSPKDG GLGDVRYRYC LTTLTVPQRC
460 470 480 490 500
LGFARRRVGR GGRVVLDRAH SDYDSMFHHL DLDMLSSPQP SPVNQFANTS
510 520 530 540 550
EPNTSDRSSS KDLSQILVDI KSCRWRHFRP RTPSLPDSDS GELSSRKLHR
560 570 580 590 600
SISRAGAAQP GAHTCSTSTQ NRSSSGSAHC AFTAEQYQQH QQQLALMQQQ
610 620 630 640 650
QLAQTQQQQQ ANSSSSAAAQ QGFVSKTLDS ASAQFAASAL MTSEQLLGFK
660 670 680 690 700
VKDDVVLGLG VNGVLPASGV YKGLHLSSTT PTALVHTSPS TAGSTLLQPS
710 720 730 740 750
NITQTSGSHS SLSHQVTAAS SATTQVLFGN NIRLTVPSSV PTVNSVTPIN
760 770 780 790 800
ARHIPRTLSA VPPSALKLAA AANCQVSKVP SSSSVDSVPR ENHESEKPAL
810
NNIADNTVAM EVT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ6PBH3 | Q6PBH3_MOUSE | Enhancer of polycomb homolog 1 | Epc1 | 61 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012878 | 1 – 51 | MSKLS…KEEES → M in isoform 2. 1 PublicationAdd BLAST | 51 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF079765 mRNA Translation: AAC64272.1 AK040254 mRNA Translation: BAC30552.1 BC138622 mRNA Translation: AAI38623.1 BC138623 mRNA Translation: AAI38624.1 |
CCDSi | CCDS29041.1 [Q8C9X6-1] CCDS37726.1 [Q8C9X6-2] |
RefSeqi | NP_001263279.1, NM_001276350.1 NP_031961.1, NM_007935.2 [Q8C9X6-2] NP_081773.1, NM_027497.3 [Q8C9X6-1] |
Genome annotation databases
Ensembli | ENSMUST00000028100; ENSMUSP00000028100; ENSMUSG00000024240 [Q8C9X6-1] ENSMUST00000115870; ENSMUSP00000111536; ENSMUSG00000024240 [Q8C9X6-2] |
GeneIDi | 13831 |
KEGGi | mmu:13831 |
UCSCi | uc008dzi.2, mouse [Q8C9X6-1] uc008dzj.2, mouse [Q8C9X6-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF079765 mRNA Translation: AAC64272.1 AK040254 mRNA Translation: BAC30552.1 BC138622 mRNA Translation: AAI38623.1 BC138623 mRNA Translation: AAI38624.1 |
CCDSi | CCDS29041.1 [Q8C9X6-1] CCDS37726.1 [Q8C9X6-2] |
RefSeqi | NP_001263279.1, NM_001276350.1 NP_031961.1, NM_007935.2 [Q8C9X6-2] NP_081773.1, NM_027497.3 [Q8C9X6-1] |
3D structure databases
AlphaFoldDBi | Q8C9X6 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 199466, 11 interactors |
ComplexPortali | CPX-747, Piccolo NuA4 histone acetyltransferase complex CPX-990, NuA4 histone acetyltransferase complex |
IntActi | Q8C9X6, 5 interactors |
MINTi | Q8C9X6 |
STRINGi | 10090.ENSMUSP00000028100 |
PTM databases
iPTMneti | Q8C9X6 |
PhosphoSitePlusi | Q8C9X6 |
Proteomic databases
EPDi | Q8C9X6 |
jPOSTi | Q8C9X6 |
MaxQBi | Q8C9X6 |
PaxDbi | Q8C9X6 |
PRIDEi | Q8C9X6 |
ProteomicsDBi | 275930 [Q8C9X6-1] 275931 [Q8C9X6-2] |
Protocols and materials databases
Antibodypediai | 26401, 87 antibodies from 20 providers |
DNASUi | 13831 |
Genome annotation databases
Ensembli | ENSMUST00000028100; ENSMUSP00000028100; ENSMUSG00000024240 [Q8C9X6-1] ENSMUST00000115870; ENSMUSP00000111536; ENSMUSG00000024240 [Q8C9X6-2] |
GeneIDi | 13831 |
KEGGi | mmu:13831 |
UCSCi | uc008dzi.2, mouse [Q8C9X6-1] uc008dzj.2, mouse [Q8C9X6-2] |
Organism-specific databases
CTDi | 80314 |
MGIi | MGI:1278322, Epc1 |
VEuPathDBi | HostDB:ENSMUSG00000024240 |
Phylogenomic databases
eggNOGi | KOG2261, Eukaryota |
GeneTreei | ENSGT00940000155003 |
HOGENOMi | CLU_012781_0_0_1 |
InParanoidi | Q8C9X6 |
OMAi | XENHESE |
OrthoDBi | 957492at2759 |
PhylomeDBi | Q8C9X6 |
TreeFami | TF106438 |
Enzyme and pathway databases
Reactomei | R-MMU-8953750, Transcriptional Regulation by E2F6 |
Miscellaneous databases
BioGRID-ORCSi | 13831, 3 hits in 75 CRISPR screens |
ChiTaRSi | Epc1, mouse |
PROi | PR:Q8C9X6 |
RNActi | Q8C9X6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024240, Expressed in pituitary gland and 307 other tissues |
ExpressionAtlasi | Q8C9X6, baseline and differential |
Genevisiblei | Q8C9X6, MM |
Family and domain databases
InterProi | View protein in InterPro IPR024943, Enhancer_polycomb IPR019542, Enhancer_polycomb-like_N IPR009607, Enhancer_polycomb_C |
PANTHERi | PTHR14898, PTHR14898, 1 hit |
Pfami | View protein in Pfam PF06752, E_Pc_C, 1 hit PF10513, EPL1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EPC1_MOUSE | |
Accessioni | Q8C9X6Primary (citable) accession number: Q8C9X6 Secondary accession number(s): B2RRY2, Q9Z299 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 15, 2005 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families