Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

A disintegrin and metalloproteinase with thrombospondin motifs 2

Gene

Adamts2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves the propeptides of type I and II collagen prior to fibril assembly. Does not act on type III collagen. May also play a role in development that is independent of its role in collagen biosynthesis (By similarity).By similarity

Catalytic activityi

Cleaves the N-propeptide of collagen chain alpha-1(I) at Pro-|-Gln and of alpha-1(II) and alpha-2(I) at Ala-|-Gln.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi409Zinc; catalyticPROSITE-ProRule annotation1
Active sitei410PROSITE-ProRule annotation1
Metal bindingi413Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi419Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • metalloendopeptidase activity Source: InterPro
  • peptidase activity Source: MGI
  • zinc ion binding Source: InterPro

GO - Biological processi

  • collagen catabolic process Source: UniProtKB-KW
  • collagen fibril organization Source: MGI
  • lung development Source: MGI
  • protein processing Source: MGI
  • skin development Source: MGI
  • spermatogenesis Source: MGI

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processCollagen degradation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1650814 Collagen biosynthesis and modifying enzymes
R-MMU-5173214 O-glycosylation of TSR domain-containing proteins

Protein family/group databases

MEROPSiM12.301

Names & Taxonomyi

Protein namesi
Recommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 2 (EC:3.4.24.14)
Short name:
ADAM-TS 2
Short name:
ADAM-TS2
Short name:
ADAMTS-2
Alternative name(s):
Procollagen I N-proteinase
Short name:
PC I-NP
Procollagen I/II amino propeptide-processing enzyme
Procollagen N-endopeptidase
Short name:
pNPI
Gene namesi
Name:Adamts2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1347356 Adamts2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
PropeptideiPRO_000002916129 – 260By similarityAdd BLAST232
ChainiPRO_0000029160261 – 1213A disintegrin and metalloproteinase with thrombospondin motifs 2Add BLAST953

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi111N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi252N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi344 ↔ 393By similarity
Disulfide bondi387 ↔ 466By similarity
Disulfide bondi426 ↔ 452By similarity
Disulfide bondi493 ↔ 518By similarity
Disulfide bondi504 ↔ 527By similarity
Disulfide bondi513 ↔ 546By similarity
Disulfide bondi540 ↔ 551By similarity
Disulfide bondi574 ↔ 611By similarity
Disulfide bondi578 ↔ 616By similarity
Disulfide bondi589 ↔ 601By similarity
Glycosylationi949N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi950N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi988 ↔ 1024By similarity
Disulfide bondi992 ↔ 1029By similarity
Glycosylationi994N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1003 ↔ 1013By similarity
Glycosylationi1032N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1099N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1147N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1152N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ8C9W3
PaxDbiQ8C9W3
PRIDEiQ8C9W3

PTM databases

iPTMnetiQ8C9W3
PhosphoSitePlusiQ8C9W3

Expressioni

Gene expression databases

BgeeiENSMUSG00000036545
GenevisibleiQ8C9W3 MM

Interactioni

Subunit structurei

May belong to a multimeric complex. Binds specifically to collagen type XIV (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040171

Structurei

3D structure databases

ProteinModelPortaliQ8C9W3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini267 – 471Peptidase M12BPROSITE-ProRule annotationAdd BLAST205
Domaini480 – 560DisintegrinAdd BLAST81
Domaini561 – 617TSP type-1 1PROSITE-ProRule annotationAdd BLAST57
Domaini855 – 913TSP type-1 2PROSITE-ProRule annotationAdd BLAST59
Domaini915 – 975TSP type-1 3PROSITE-ProRule annotationAdd BLAST61
Domaini976 – 1030TSP type-1 4PROSITE-ProRule annotationAdd BLAST55
Domaini1060 – 1098PLACPROSITE-ProRule annotationAdd BLAST39

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni723 – 851SpacerAdd BLAST129

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi692 – 694Cell attachment siteSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi618 – 722Cys-richAdd BLAST105

Domaini

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410INDA Eukaryota
ENOG410XSRH LUCA
GeneTreeiENSGT00900000140815
HOGENOMiHOG000034222
HOVERGENiHBG004314
InParanoidiQ8C9W3
KOiK08618
OMAiGVEWEYR
OrthoDBiEOG091G0790
PhylomeDBiQ8C9W3
TreeFamiTF313537

Family and domain databases

Gene3Di2.20.100.10, 4 hits
3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR010294 ADAM_spacer1
IPR024079 MetalloPept_cat_dom_sf
IPR013275 Pept_M12B_ADAM-TS2
IPR001590 Peptidase_M12B
IPR002870 Peptidase_M12B_N
IPR010909 PLAC
IPR000884 TSP1_rpt
IPR036383 TSP1_rpt_sf
PfamiView protein in Pfam
PF05986 ADAM_spacer1, 1 hit
PF01562 Pep_M12B_propep, 1 hit
PF01421 Reprolysin, 1 hit
PF00090 TSP_1, 4 hits
PRINTSiPR01859 ADAMTS2
SMARTiView protein in SMART
SM00209 TSP1, 4 hits
SUPFAMiSSF82895 SSF82895, 4 hits
PROSITEiView protein in PROSITE
PS50215 ADAM_MEPRO, 1 hit
PS50900 PLAC, 1 hit
PS50092 TSP1, 4 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8C9W3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPPAGAARR LLCPALLLLL LPPPPLLLLP PPPASVRLVA ATEPPGGPPG
60 70 80 90 100
QGAERILAVP VRTDAQGRLV SHVVSLETAG AGVRARRAAL DQTSGLPGGA
110 120 130 140 150
AQDPGGRLFY NLTVFGRDLH LRLRPNARLV APGATVEWQG ETGDTRVEPL
160 170 180 190 200
LGSCLYVGDV ADLPKASSVA LSNCDGLAGL IRMEEEEFFI EPLEKGQTDQ
210 220 230 240 250
EAEQGRVHVV YRRPPTPKPP PVSEPQALDT GVSQGNLDSL SRALGVLEER
260 270 280 290 300
INSSRRRVRR HATDDDYNIE VLLGVDDSVV QFHGKEHVQK YLLTLMNIVN
310 320 330 340 350
EIYHDESLGA HINVVLVRII LLSHAKSMSL IEIGNPSQSL ENVCRWAYLQ
360 370 380 390 400
QKPDTDHDEY HDHAIFLTRQ DFGPSGMQGY APVTGMCHPV RSCTLNHEDG
410 420 430 440 450
FSSAFVVAHE TGHVLGMEHD GQGNRCGDEV RLGSIMAPLV QAAFHRFHWS
460 470 480 490 500
RCSQQELSRY LHSYDCLRDD PFAHDWPALP QLPGLHYSMN EQCRFDFGLG
510 520 530 540 550
YMMCTAFRTF DPCKQLWCSH PDNPYFCKTK KGPPLDGTMC APGKHCFKGH
560 570 580 590 600
CIWLTPDILK RDGNWGAWTP FGSCSRTCGT GVKFRTRQCD NPHPANGGRT
610 620 630 640 650
CSGLAYDFQL CNPQDCPNSL ADFREEQCQQ WDLYFEHGDV QHHWLPHEHR
660 670 680 690 700
DAKERCHLYC ESKETGEVVS MKRMVHDGTR CSYKDAFSLC VRGDCRKVGC
710 720 730 740 750
DGVIGSRKQE DKCGVCGGDN THCKVVKGTF TRSPRKQDYI KMFEIPAGAR
760 770 780 790 800
HLLIQEADTT SHHLSVKNLE TGKFILNEEN HLDPNSRSFI AMGVEWEYRN
810 820 830 840 850
EDERETLQTI GPLHGTITVL VIPEGDTRIS LTYKYMIHED SLNVDDNNVL
860 870 880 890 900
EDDAVRHEWA LKKWSPCSKP CGGGSQFTKY GCRRRLDSKM VHRAFCSALA
910 920 930 940 950
KPKAIRRACN PQECSQPVWV TGEWEPCTQS CGRTGMQVRS VRCIQPLHNN
960 970 980 990 1000
TTRSVHTKHC NDHRPESRRA CNRELCPGRW RAGSWSQCSV TCGNGTQERP
1010 1020 1030 1040 1050
VLCRTADDNF GVCREERPET ARICRLAPCP RNGSDPSKKS YVVQWLSRPD
1060 1070 1080 1090 1100
PDSPIQKISS KDQCQGDKSM FCRMEVLSRY CSIPSYNKLC CKSCNPPRNL
1110 1120 1130 1140 1150
SNTEDGGVEP PPGKHNDIDV FMPTLPGPTV ATQVQPSPGP PLEAPLNVSS
1160 1170 1180 1190 1200
TNATEDHPET NAVDVPYKIH GVDEEVPSPN LIPRRPSLYV KTRNQRIQEL
1210
INAVQRKEKP GKF
Length:1,213
Mass (Da):135,299
Last modified:June 20, 2003 - v2
Checksum:iB27431E00443EDB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK040370 mRNA Translation: BAC30572.1
BC046456 mRNA Translation: AAH46456.1
CCDSiCCDS24636.1
RefSeqiNP_783574.1, NM_175643.3
UniGeneiMm.110597
Mm.339048

Genome annotation databases

EnsembliENSMUST00000040523; ENSMUSP00000040171; ENSMUSG00000036545
GeneIDi216725
KEGGimmu:216725
UCSCiuc007iso.2 mouse

Similar proteinsi

Entry informationi

Entry nameiATS2_MOUSE
AccessioniPrimary (citable) accession number: Q8C9W3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: June 20, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health