UniProtKB - Q8C854 (MYEF2_MOUSE)
Protein
Myelin expression factor 2
Gene
Myef2
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional repressor of the myelin basic protein gene (MBP). Binds to the proximal MB1 element 5'-TTGTCC-3' of the MBP promoter. Its binding to MB1 and function are inhibited by PURA.3 Publications
GO - Molecular functioni
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- mRNA binding Source: GO_Central
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- single-stranded DNA binding Source: InterPro
GO - Biological processi
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of mRNA stability involved in response to oxidative stress Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor, RNA-binding |
Biological process | Transcription |
Names & Taxonomyi
Protein namesi | Recommended name: Myelin expression factor 2Short name: MEF-2 Short name: MyEF-2 |
Gene namesi | Name:Myef2 Synonyms:Kiaa1341, Mef2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104592, Myef2 |
Subcellular locationi
Nucleus
Nucleus
- nucleus Source: MGI
- post-mRNA release spliceosomal complex Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- ribonucleoprotein complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081656 | 1 – 591 | Myelin expression factor 2Add BLAST | 591 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 44 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 397 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 417 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 422 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8C854 |
MaxQBi | Q8C854 |
PaxDbi | Q8C854 |
PeptideAtlasi | Q8C854 |
PRIDEi | Q8C854 |
PTM databases
iPTMneti | Q8C854 |
PhosphoSitePlusi | Q8C854 |
SwissPalmi | Q8C854 |
Expressioni
Tissue specificityi
Highly expressed in the brain.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000027201, Expressed in ventricular zone and 281 other tissues |
ExpressionAtlasi | Q8C854, baseline and differential |
Genevisiblei | Q8C854, MM |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 201640, 17 interactors |
IntActi | Q8C854, 8 interactors |
MINTi | Q8C854 |
STRINGi | 10090.ENSMUSP00000123088 |
Miscellaneous databases
RNActi | Q8C854, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 91 – 169 | RRM 1PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 224 – 301 | RRM 2PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 514 – 590 | RRM 3PROSITE-ProRule annotationAdd BLAST | 77 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 321 – 513 | Gly-richAdd BLAST | 193 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0118, Eukaryota |
GeneTreei | ENSGT00940000157397 |
HOGENOMi | CLU_019566_1_0_1 |
InParanoidi | Q8C854 |
OMAi | GTRSKGC |
OrthoDBi | 1174365at2759 |
PhylomeDBi | Q8C854 |
TreeFami | TF313406 |
Family and domain databases
CDDi | cd12658, RRM1_MYEF2, 1 hit cd12662, RRM3_MYEF2, 1 hit |
Gene3Di | 3.30.70.330, 3 hits |
InterProi | View protein in InterPro IPR034632, MYEF2 IPR034630, MYEF2_RRM1 IPR034631, MYEF2_RRM3 IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
PANTHERi | PTHR23003:SF15, PTHR23003:SF15, 1 hit |
Pfami | View protein in Pfam PF00076, RRM_1, 3 hits |
SMARTi | View protein in SMART SM00360, RRM, 3 hits |
SUPFAMi | SSF54928, SSF54928, 3 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8C854-4) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADADKSEAA AGDDGSQQQP AEPRRDTHPG EPEKPPRSSA NGVKMENDES
60 70 80 90 100
VKEEKSDLKE KSTGNKKANR FHPYSKDKNS GTGEKKGPNR NRVFISNIPY
110 120 130 140 150
DMKWQAIKDL MREKVGEVTY VELFKDAEGK SRGCGVVEFK DEEFVKKALE
160 170 180 190 200
TMNKYDLSGR PLNIKEDPDG ENARRALQRT GTSFQGSHAS DVGSGLVNLP
210 220 230 240 250
PSILNNPNIP PEVISNLQAG RLGSTIFVAN LDFKVGWKKL KEVFSIAGTV
260 270 280 290 300
KRADIKEDKD GKSRGMGTVT FEQAIEAVQA ISMFNGQFLF DRPMHVKMDD
310 320 330 340 350
KSVPHEDYRS HDSKTSQLPR GLGGIGMGLG PGGQPISASQ LNITGVMGNL
360 370 380 390 400
GPSGMGMDGP GFGGVNRIGG GVGFGGLEAM NSMAGFGGVG RMGELYRGAM
410 420 430 440 450
TSSMERDFGR GDIGLSRGFG DSFGRLGSAM IGGFAGRIGA SNMGPVGTGI
460 470 480 490 500
SGSMSGMSTV TGGMGMGLDR MSSSFDRMGP GIGAILERSI DVDRGFLSGP
510 520 530 540 550
MGSGMRDRLG SKGNQIFVRN LPFDLTWQKL KEKFSQCGHV MFAEIKMENG
560 570 580 590
KSKGCGTVRF ESAESAEKAC RIMNGIKISG REIDVRLDRN A
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2ATP5 | A2ATP5_MOUSE | Myelin expression factor 2 | Myef2 | 550 | Annotation score: | ||
F6XJA1 | F6XJA1_MOUSE | Myelin expression factor 2 | Myef2 | 446 | Annotation score: | ||
A2ATP6 | A2ATP6_MOUSE | Myelin expression factor 2 | Myef2 | 567 | Annotation score: | ||
G8JL68 | G8JL68_MOUSE | Myelin expression factor 2 | Myef2 | 530 | Annotation score: |
Sequence cautioni
The sequence AAH60946 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC98146 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 341 | L → R in AAA78270 (PubMed:7534248).Curated | 1 | |
Sequence conflicti | 537 | C → L in AAA78270 (PubMed:7534248).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013455 | 354 – 370 | Missing in isoform 2. 2 PublicationsAdd BLAST | 17 | |
Alternative sequenceiVSP_013456 | 394 – 451 | ELYRG…GTGIS → G in isoform 4. 1 PublicationAdd BLAST | 58 | |
Alternative sequenceiVSP_013457 | 427 – 451 | GSAMI…GTGIS → G in isoform 3. 1 PublicationAdd BLAST | 25 | |
Alternative sequenceiVSP_013458 | 511 – 512 | SK → RN in isoform 3. 1 Publication | 2 | |
Alternative sequenceiVSP_013459 | 513 – 591 | Missing in isoform 3. 1 PublicationAdd BLAST | 79 | |
Alternative sequenceiVSP_013460 | 539 – 555 | HVMFA…KSKGC → QINRDSKTHSNCGQHHS in isoform 4. 1 PublicationAdd BLAST | 17 | |
Alternative sequenceiVSP_013461 | 556 – 591 | Missing in isoform 4. 1 PublicationAdd BLAST | 36 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK048404 mRNA Translation: BAC33325.1 AK129336 mRNA Translation: BAC98146.1 Different initiation. BC060946 mRNA Translation: AAH60946.1 Different initiation. AF483504 mRNA Translation: AAL90778.1 AF483505 mRNA Translation: AAL90779.1 U13262 mRNA Translation: AAA78270.1 |
CCDSi | CCDS50690.1 [Q8C854-3] CCDS50691.1 [Q8C854-4] |
PIRi | A56704 |
RefSeqi | NP_001155889.1, NM_001162417.1 [Q8C854-4] XP_006498947.1, XM_006498884.3 [Q8C854-3] XP_017171620.1, XM_017316131.1 [Q8C854-4] XP_017171621.1, XM_017316132.1 [Q8C854-4] XP_017171623.1, XM_017316134.1 [Q8C854-4] |
Genome annotation databases
Ensembli | ENSMUST00000061419; ENSMUSP00000058811; ENSMUSG00000049230 [Q8C854-2] ENSMUST00000067780; ENSMUSP00000066312; ENSMUSG00000027201 [Q8C854-3] ENSMUST00000110501; ENSMUSP00000106127; ENSMUSG00000027201 [Q8C854-3] ENSMUST00000152367; ENSMUSP00000123088; ENSMUSG00000027201 [Q8C854-4] |
GeneIDi | 17876 |
KEGGi | mmu:17876 |
UCSCi | uc008mbw.2, mouse [Q8C854-4] uc008mby.2, mouse [Q8C854-3] uc012cdb.1, mouse [Q8C854-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK048404 mRNA Translation: BAC33325.1 AK129336 mRNA Translation: BAC98146.1 Different initiation. BC060946 mRNA Translation: AAH60946.1 Different initiation. AF483504 mRNA Translation: AAL90778.1 AF483505 mRNA Translation: AAL90779.1 U13262 mRNA Translation: AAA78270.1 |
CCDSi | CCDS50690.1 [Q8C854-3] CCDS50691.1 [Q8C854-4] |
PIRi | A56704 |
RefSeqi | NP_001155889.1, NM_001162417.1 [Q8C854-4] XP_006498947.1, XM_006498884.3 [Q8C854-3] XP_017171620.1, XM_017316131.1 [Q8C854-4] XP_017171621.1, XM_017316132.1 [Q8C854-4] XP_017171623.1, XM_017316134.1 [Q8C854-4] |
3D structure databases
SMRi | Q8C854 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201640, 17 interactors |
IntActi | Q8C854, 8 interactors |
MINTi | Q8C854 |
STRINGi | 10090.ENSMUSP00000123088 |
PTM databases
iPTMneti | Q8C854 |
PhosphoSitePlusi | Q8C854 |
SwissPalmi | Q8C854 |
Proteomic databases
EPDi | Q8C854 |
MaxQBi | Q8C854 |
PaxDbi | Q8C854 |
PeptideAtlasi | Q8C854 |
PRIDEi | Q8C854 |
Genome annotation databases
Ensembli | ENSMUST00000061419; ENSMUSP00000058811; ENSMUSG00000049230 [Q8C854-2] ENSMUST00000067780; ENSMUSP00000066312; ENSMUSG00000027201 [Q8C854-3] ENSMUST00000110501; ENSMUSP00000106127; ENSMUSG00000027201 [Q8C854-3] ENSMUST00000152367; ENSMUSP00000123088; ENSMUSG00000027201 [Q8C854-4] |
GeneIDi | 17876 |
KEGGi | mmu:17876 |
UCSCi | uc008mbw.2, mouse [Q8C854-4] uc008mby.2, mouse [Q8C854-3] uc012cdb.1, mouse [Q8C854-2] |
Organism-specific databases
CTDi | 50804 |
MGIi | MGI:104592, Myef2 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG0118, Eukaryota |
GeneTreei | ENSGT00940000157397 |
HOGENOMi | CLU_019566_1_0_1 |
InParanoidi | Q8C854 |
OMAi | GTRSKGC |
OrthoDBi | 1174365at2759 |
PhylomeDBi | Q8C854 |
TreeFami | TF313406 |
Miscellaneous databases
BioGRID-ORCSi | 17876, 1 hit in 17 CRISPR screens |
ChiTaRSi | Myef2, mouse |
PROi | PR:Q8C854 |
RNActi | Q8C854, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027201, Expressed in ventricular zone and 281 other tissues |
ExpressionAtlasi | Q8C854, baseline and differential |
Genevisiblei | Q8C854, MM |
Family and domain databases
CDDi | cd12658, RRM1_MYEF2, 1 hit cd12662, RRM3_MYEF2, 1 hit |
Gene3Di | 3.30.70.330, 3 hits |
InterProi | View protein in InterPro IPR034632, MYEF2 IPR034630, MYEF2_RRM1 IPR034631, MYEF2_RRM3 IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
PANTHERi | PTHR23003:SF15, PTHR23003:SF15, 1 hit |
Pfami | View protein in Pfam PF00076, RRM_1, 3 hits |
SMARTi | View protein in SMART SM00360, RRM, 3 hits |
SUPFAMi | SSF54928, SSF54928, 3 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MYEF2_MOUSE | |
Accessioni | Q8C854Primary (citable) accession number: Q8C854 Secondary accession number(s): Q60690 Q8QZZ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot