Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin-like modifier-activating enzyme 6

Gene

Uba6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34 (By similarity). Essential for embryonic development. Required for UBD/FAT10 conjugation.By similarity1 Publication

Catalytic activityi

ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei470ATP; via amide nitrogenBy similarity1
Binding sitei497ATPBy similarity1
Binding sitei508ATPBy similarity1
Binding sitei521ATPBy similarity1
Active sitei625Glycyl thioester intermediate1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi569 – 570ATPBy similarity2

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • FAT10 activating enzyme activity Source: UniProtKB
  • ubiquitin activating enzyme activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like modifier-activating enzyme 6 (EC:6.2.1.45By similarity)
Short name:
Ubiquitin-activating enzyme 6
Alternative name(s):
Ubiquitin-activating enzyme E1-like protein 2
Short name:
E1-L2
Gene namesi
Name:Uba6
Synonyms:Ube1l2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1913894 Uba6

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality at early stage.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002777981 – 1053Ubiquitin-like modifier-activating enzyme 6Add BLAST1053

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei54PhosphothreonineBy similarity1
Modified residuei544N6-acetyllysineBy similarity1
Modified residuei729N6-acetyllysineCombined sources1
Modified residuei737PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8C7R4
MaxQBiQ8C7R4
PaxDbiQ8C7R4
PeptideAtlasiQ8C7R4
PRIDEiQ8C7R4

PTM databases

iPTMnetiQ8C7R4
PhosphoSitePlusiQ8C7R4
SwissPalmiQ8C7R4

Expressioni

Gene expression databases

BgeeiENSMUSG00000035898 Expressed in 242 organ(s), highest expression level in ear vesicle
CleanExiMM_UBA6
ExpressionAtlasiQ8C7R4 baseline and differential
GenevisibleiQ8C7R4 MM

Interactioni

Subunit structurei

Forms a thioester with UBD in cells stimulated with tumor necrosis factor-alpha (TNFa) and interferon-gamma (IFNg) (PubMed:17889673).1 Publication

Protein-protein interaction databases

BioGridi231114, 3 interactors
IntActiQ8C7R4, 3 interactors
MINTiQ8C7R4
STRINGi10090.ENSMUSP00000035328

Structurei

3D structure databases

ProteinModelPortaliQ8C7R4
SMRiQ8C7R4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family.Curated

Phylogenomic databases

eggNOGiKOG2012 Eukaryota
COG0476 LUCA
GeneTreeiENSGT00390000016689
HOGENOMiHOG000167329
HOVERGENiHBG054199
InParanoidiQ8C7R4
KOiK10699
OMAiHIQKPKS
OrthoDBiEOG091G0130
PhylomeDBiQ8C7R4
TreeFamiTF300586

Family and domain databases

Gene3Di3.10.290.60, 1 hit
InterProiView protein in InterPro
IPR032420 E1_4HB
IPR032418 E1_FCCH
IPR000594 ThiF_NAD_FAD-bd
IPR018965 Ub-activating_enz_E1_C
IPR038252 UBA_E1_C_sf
IPR019572 UBA_E1_Cys
IPR035985 Ubiquitin-activating_enz
IPR018075 UBQ-activ_enz_E1
IPR000011 UBQ/SUMO-activ_enz_E1-like
PfamiView protein in Pfam
PF16191 E1_4HB, 1 hit
PF16190 E1_FCCH, 1 hit
PF09358 E1_UFD, 1 hit
PF00899 ThiF, 2 hits
PF10585 UBA_e1_thiolCys, 1 hit
PRINTSiPR01849 UBIQUITINACT
SMARTiView protein in SMART
SM00985 UBA_e1_C, 1 hit
SUPFAMiSSF69572 SSF69572, 2 hits
TIGRFAMsiTIGR01408 Ube1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8C7R4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MERSEPLAVL SCEEASCSSW GACGASKNLP TMTTESLEID DGLYSRQRYV
60 70 80 90 100
LGDTAMQKMA KSCVFLSGMG GLGVEIAKNL VLAGIKALTI HDTKKCQAWD
110 120 130 140 150
LGTNFFLCED DVVNERNRAE AVLHRIAELN PYVQVSSSSA PLDETTDLSF
160 170 180 190 200
LEKYQCVVLT EIKLTLQKKI NNFCHSHCPP IKFISADVHG IWSRLFCDFG
210 220 230 240 250
DEFEVSDTTG EEPKEIFISN ITQANPGIVT CLESHPHKLE TGQFLTFREI
260 270 280 290 300
HGMTGLNGSV QQITVISPFS FSIGDTTKLD PYLHGGIAVQ VKTPKTFCFE
310 320 330 340 350
PLESQIKHPR CLIADFSKPE APLEIHLAML ALDQFQENYN RKPNIRCQQD
360 370 380 390 400
SDELLKLTVS INETLEEKPE VNADIVHWLS WTAQGFLPPL AAAVGGVASQ
410 420 430 440 450
EVLKAVTGKF SPLCQWLYLE AADTVESLGN PGHEEFLPRG DRYDAIRACI
460 470 480 490 500
GNTLCQKLQN LNIFLVGCGA IGCEMLKNFA LLGVGTGREK GMVTVTDPDL
510 520 530 540 550
IEKSNLNRQF LFRPHHIQKP KSYTAAEATL KINPQLKIDA HLNKVCPATE
560 570 580 590 600
SIYSDEFYTK QDIIITALDN VEARRYVDSR CLANLRPLLD SGTMGTKGHT
610 620 630 640 650
EIIVPQLTES YNSHRDPPEE EIPFCTLKSF PAAIEHTIQW ARDKFESSFS
660 670 680 690 700
HKPSLFNKFW QAYPSAEDVL QKIQNGQSLE GCFQVIKLLS RRPRIWSQCV
710 720 730 740 750
ELARLKFEKY FNHKALQLLH CFPLETRLKD GSLFWQSPKR PPSPIKFDLN
760 770 780 790 800
EPLHLSFLQS AAKLYATVYC IPFSEKDLSV NSLMDILSEV KIEEFKPSNK
810 820 830 840 850
VVQTDETARK PDHVPVSSED ERNAVFQLEE ALSSNKATKS DLQMTVLSFE
860 870 880 890 900
KDDDRNGHID FITAASNLRA KMYSIEPADR FKTKRIAGKI IPAIATSTAA
910 920 930 940 950
VSGLVALEMI KVAGGYPFDA YKNCFLNLAI PIIVFTETSE VRKTEIRNGI
960 970 980 990 1000
SFTIWDRWTV HGKEDFTLSD FINAVKENYG IEPTMVVQGV KMLYVPVMPG
1010 1020 1030 1040 1050
HAKRLKLTMH KLVKPSTEKK YVDLTVSFAP DADGDEDLPG PPVRYYFSHD

TNE
Length:1,053
Mass (Da):117,966
Last modified:March 1, 2003 - v1
Checksum:i90529575F77DE09B
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z061D3Z061_MOUSE
Ubiquitin-like modifier-activating ...
Uba6
1,022Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049603 mRNA Translation: BAC33836.1
BC063048 mRNA Translation: AAH63048.1
CCDSiCCDS19378.1
RefSeqiNP_766300.1, NM_172712.2
UniGeneiMm.34012
Mm.392216
Mm.393083
Mm.491297

Genome annotation databases

EnsembliENSMUST00000039373; ENSMUSP00000035328; ENSMUSG00000035898
GeneIDi231380
KEGGimmu:231380
UCSCiuc008xxj.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049603 mRNA Translation: BAC33836.1
BC063048 mRNA Translation: AAH63048.1
CCDSiCCDS19378.1
RefSeqiNP_766300.1, NM_172712.2
UniGeneiMm.34012
Mm.392216
Mm.393083
Mm.491297

3D structure databases

ProteinModelPortaliQ8C7R4
SMRiQ8C7R4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231114, 3 interactors
IntActiQ8C7R4, 3 interactors
MINTiQ8C7R4
STRINGi10090.ENSMUSP00000035328

PTM databases

iPTMnetiQ8C7R4
PhosphoSitePlusiQ8C7R4
SwissPalmiQ8C7R4

Proteomic databases

EPDiQ8C7R4
MaxQBiQ8C7R4
PaxDbiQ8C7R4
PeptideAtlasiQ8C7R4
PRIDEiQ8C7R4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039373; ENSMUSP00000035328; ENSMUSG00000035898
GeneIDi231380
KEGGimmu:231380
UCSCiuc008xxj.2 mouse

Organism-specific databases

CTDi55236
MGIiMGI:1913894 Uba6

Phylogenomic databases

eggNOGiKOG2012 Eukaryota
COG0476 LUCA
GeneTreeiENSGT00390000016689
HOGENOMiHOG000167329
HOVERGENiHBG054199
InParanoidiQ8C7R4
KOiK10699
OMAiHIQKPKS
OrthoDBiEOG091G0130
PhylomeDBiQ8C7R4
TreeFamiTF300586

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

PROiPR:Q8C7R4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035898 Expressed in 242 organ(s), highest expression level in ear vesicle
CleanExiMM_UBA6
ExpressionAtlasiQ8C7R4 baseline and differential
GenevisibleiQ8C7R4 MM

Family and domain databases

Gene3Di3.10.290.60, 1 hit
InterProiView protein in InterPro
IPR032420 E1_4HB
IPR032418 E1_FCCH
IPR000594 ThiF_NAD_FAD-bd
IPR018965 Ub-activating_enz_E1_C
IPR038252 UBA_E1_C_sf
IPR019572 UBA_E1_Cys
IPR035985 Ubiquitin-activating_enz
IPR018075 UBQ-activ_enz_E1
IPR000011 UBQ/SUMO-activ_enz_E1-like
PfamiView protein in Pfam
PF16191 E1_4HB, 1 hit
PF16190 E1_FCCH, 1 hit
PF09358 E1_UFD, 1 hit
PF00899 ThiF, 2 hits
PF10585 UBA_e1_thiolCys, 1 hit
PRINTSiPR01849 UBIQUITINACT
SMARTiView protein in SMART
SM00985 UBA_e1_C, 1 hit
SUPFAMiSSF69572 SSF69572, 2 hits
TIGRFAMsiTIGR01408 Ube1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUBA6_MOUSE
AccessioniPrimary (citable) accession number: Q8C7R4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again