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Protein

Vacuolar protein sorting-associated protein 52 homolog

Gene

Vps52

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.By similarity

GO - Molecular functioni

  • Rab GTPase binding Source: GO_Central
  • syntaxin binding Source: MGI

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-6811440 Retrograde transport at the Trans-Golgi-Network

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 52 homolog
Gene namesi
Name:Vps52
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1330304 Vps52

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002133162 – 723Vacuolar protein sorting-associated protein 52 homologAdd BLAST722

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei355PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8C754
PaxDbiQ8C754
PeptideAtlasiQ8C754
PRIDEiQ8C754

PTM databases

iPTMnetiQ8C754
PhosphoSitePlusiQ8C754

Expressioni

Gene expression databases

BgeeiENSMUSG00000024319 Expressed in 217 organ(s), highest expression level in ear vesicle
CleanExiMM_VPS52
ExpressionAtlasiQ8C754 baseline and differential
GenevisibleiQ8C754 MM

Interactioni

Subunit structurei

Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. Component of the endosome-associated retrograde protein (EARP) complex, composed of VPS51, VPS52, VPS53 and VPS50/Syndetin. EIPR1 interacts with both EARP and GARP complexes and mediates the recruitment of the GARP complex to the trans-Golgi network. Interacts with RAB6A and STX10. Interacts with UHRF1BP1L.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025178

Structurei

3D structure databases

ProteinModelPortaliQ8C754
SMRiQ8C754
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili107 – 127Sequence analysisAdd BLAST21
Coiled coili194 – 214Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the VPS52 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1961 Eukaryota
ENOG410XNZ1 LUCA
GeneTreeiENSGT00390000008815
HOGENOMiHOG000020960
HOVERGENiHBG052966
InParanoidiQ8C754
KOiK20298
OMAiYKFRKPM
OrthoDBiEOG091G02UM
PhylomeDBiQ8C754
TreeFamiTF314937

Family and domain databases

InterProiView protein in InterPro
IPR007258 Vps52
PANTHERiPTHR14190 PTHR14190, 1 hit
PfamiView protein in Pfam
PF04129 Vps52, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8C754-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAAATMAAA ARELVLRAGA SDVEEEEGPL GGGSGLQEPL QLGELDITSD
60 70 80 90 100
EFILDEVDVH IQANLEDELV KEALKTGVDL RHYSKQVELE LQQIEQKSIR
110 120 130 140 150
DYIQESENIA SLHNQITACD AVLERMEQML GAFQSDLSSI SSEIRTLQEQ
160 170 180 190 200
SGAMNIRLRN RQAVRGKLGE LVDGLVVPSA LVTAILEAPV TEPRFLEQLQ
210 220 230 240 250
ELDAKAAAVR EQEAMGTAAC ADVRGVLDRL RVKAVTKIRE FILQKIYSFR
260 270 280 290 300
KPMTNYQIPQ AALLKYRFFY QFLLGNERAT AKEIRDEYVE TLSKIYLSYY
310 320 330 340 350
RSYVGRLMKV QYEEVAEKDD LMGVEDTAKK GFFSKPSLRS RNTIFTLGTR
360 370 380 390 400
GTVISPAELE APILVPHTAQ RGEQRYPFEA LFRSQHYALL DNSCREYLFI
410 420 430 440 450
CEFFIVSGPA AHDLFHAVMG RTLSMTLKHL ESYLADCYDA IAVFLCIHIV
460 470 480 490 500
LRFRNIAAKR DVPALDRYWE QVLALLWPRF ELILEMNVQS VRSTDPQRLG
510 520 530 540 550
GLDTRPHYIT RRYAEFSSAL VSINQTIPNE RTLQLLGQLQ VEVENFVLRV
560 570 580 590 600
AAEFSSRKEQ LVFLINNYDM MLGVLMERAA DDSKEVESFQ QLLNARTQEF
610 620 630 640 650
IEELLSPPFG GLVAFVKEAE ALIERGQAER LRGEEARVTQ LIRGFGSSWK
660 670 680 690 700
ASVESLSQDV MRSFTNFRNG TSIIQGALTQ LIQLYHRFHR VLSQPQLRAL
710 720
PARAELINIH HLMVELKKHK PNF
Length:723
Mass (Da):82,044
Last modified:March 1, 2003 - v1
Checksum:i5209C84FCA84DF75
GO
Isoform 2 (identifier: Q8C754-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     124-191: Missing.
     681-715: Missing.

Note: No experimental confirmation available.
Show »
Length:620
Mass (Da):70,484
Checksum:iEB4F0EF343A0289D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3UY33G3UY33_MOUSE
Vacuolar protein sorting-associated...
Vps52
612Annotation score:

Sequence cautioni

The sequence AAC69899 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAC97979 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti458A → G in AAH63329 (PubMed:15489334).Curated1
Sequence conflicti722N → K in BAC34741 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016234124 – 191Missing in isoform 2. 1 PublicationAdd BLAST68
Alternative sequenceiVSP_016235681 – 715Missing in isoform 2. 1 PublicationAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051732 mRNA Translation: BAC34741.1
AK052523 mRNA Translation: BAC35024.1
AF100956 Genomic DNA Translation: AAC69899.1 Sequence problems.
AF110520 Genomic DNA Translation: AAC97979.1 Sequence problems.
BC063329 mRNA Translation: AAH63329.1
CCDSiCCDS37574.1 [Q8C754-1]
RefSeqiNP_766208.2, NM_172620.3 [Q8C754-1]
UniGeneiMm.448614
Mm.485758
Mm.487048

Genome annotation databases

EnsembliENSMUST00000025178; ENSMUSP00000025178; ENSMUSG00000024319 [Q8C754-1]
GeneIDi224705
KEGGimmu:224705
UCSCiuc008cam.1 mouse [Q8C754-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051732 mRNA Translation: BAC34741.1
AK052523 mRNA Translation: BAC35024.1
AF100956 Genomic DNA Translation: AAC69899.1 Sequence problems.
AF110520 Genomic DNA Translation: AAC97979.1 Sequence problems.
BC063329 mRNA Translation: AAH63329.1
CCDSiCCDS37574.1 [Q8C754-1]
RefSeqiNP_766208.2, NM_172620.3 [Q8C754-1]
UniGeneiMm.448614
Mm.485758
Mm.487048

3D structure databases

ProteinModelPortaliQ8C754
SMRiQ8C754
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025178

PTM databases

iPTMnetiQ8C754
PhosphoSitePlusiQ8C754

Proteomic databases

EPDiQ8C754
PaxDbiQ8C754
PeptideAtlasiQ8C754
PRIDEiQ8C754

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025178; ENSMUSP00000025178; ENSMUSG00000024319 [Q8C754-1]
GeneIDi224705
KEGGimmu:224705
UCSCiuc008cam.1 mouse [Q8C754-1]

Organism-specific databases

CTDi6293
MGIiMGI:1330304 Vps52

Phylogenomic databases

eggNOGiKOG1961 Eukaryota
ENOG410XNZ1 LUCA
GeneTreeiENSGT00390000008815
HOGENOMiHOG000020960
HOVERGENiHBG052966
InParanoidiQ8C754
KOiK20298
OMAiYKFRKPM
OrthoDBiEOG091G02UM
PhylomeDBiQ8C754
TreeFamiTF314937

Enzyme and pathway databases

ReactomeiR-MMU-6811440 Retrograde transport at the Trans-Golgi-Network

Miscellaneous databases

ChiTaRSiVps52 mouse
PROiPR:Q8C754
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024319 Expressed in 217 organ(s), highest expression level in ear vesicle
CleanExiMM_VPS52
ExpressionAtlasiQ8C754 baseline and differential
GenevisibleiQ8C754 MM

Family and domain databases

InterProiView protein in InterPro
IPR007258 Vps52
PANTHERiPTHR14190 PTHR14190, 1 hit
PfamiView protein in Pfam
PF04129 Vps52, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVPS52_MOUSE
AccessioniPrimary (citable) accession number: Q8C754
Secondary accession number(s): Q6P4N7
, Q8BQ15, Q9QWT6, Q9QWV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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