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Protein

Ubiquitin carboxyl-terminal hydrolase 20

Gene

Usp20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei154NucleophilePROSITE-ProRule annotation1
Active sitei645Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri28 – 92UBP-typePROSITE-ProRule annotationAdd BLAST65

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processEndocytosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5689880 Ub-specific processing proteases

Protein family/group databases

MEROPSiC19.025

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 20 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 20
Ubiquitin thioesterase 20
Ubiquitin-specific-processing protease 20
VHL-interacting deubiquitinating enzyme 2
Gene namesi
Name:Usp20
Synonyms:Kiaa1003, Vdu2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1921520 Usp20

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003904181 – 916Ubiquitin carboxyl-terminal hydrolase 20Add BLAST916

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei112PhosphoserineBy similarity1
Modified residuei132PhosphoserineCombined sources1
Modified residuei134PhosphoserineBy similarity1
Modified residuei259PhosphothreonineBy similarity1
Modified residuei306PhosphoserineBy similarity1
Modified residuei369PhosphoserineCombined sources1
Modified residuei378PhosphothreonineCombined sources1
Modified residuei410PhosphoserineBy similarity1
Modified residuei415PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8C6M1
PaxDbiQ8C6M1
PeptideAtlasiQ8C6M1
PRIDEiQ8C6M1

PTM databases

iPTMnetiQ8C6M1
PhosphoSitePlusiQ8C6M1

Expressioni

Gene expression databases

BgeeiENSMUSG00000026854
ExpressionAtlasiQ8C6M1 baseline and differential
GenevisibleiQ8C6M1 MM

Interactioni

Subunit structurei

Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with CCP110, DIO2 and HIF1A (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8C6M1, 1 interactor
STRINGi10090.ENSMUSP00000099913

Structurei

3D structure databases

ProteinModelPortaliQ8C6M1
SMRiQ8C6M1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini145 – 687USPAdd BLAST543
Domaini689 – 782DUSP 1PROSITE-ProRule annotationAdd BLAST94
Domaini791 – 894DUSP 2PROSITE-ProRule annotationAdd BLAST104

Domaini

The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue (By similarity).By similarity

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri28 – 92UBP-typePROSITE-ProRule annotationAdd BLAST65

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1870 Eukaryota
COG5560 LUCA
GeneTreeiENSGT00860000133682
HOGENOMiHOG000286031
HOVERGENiHBG054196
InParanoidiQ8C6M1
KOiK11848
OMAiLHGEQKI
OrthoDBiEOG091G02UV
PhylomeDBiQ8C6M1
TreeFamiTF352179

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR035927 DUSP-like_sf
IPR038765 Papain_like_cys_pep_sf
IPR006615 Pept_C19_DUSP
IPR001394 Peptidase_C19_UCH
IPR018200 USP_CS
IPR028889 USP_dom
IPR013083 Znf_RING/FYVE/PHD
IPR001607 Znf_UBP
PfamiView protein in Pfam
PF06337 DUSP, 2 hits
PF00443 UCH, 1 hit
PF02148 zf-UBP, 1 hit
SMARTiView protein in SMART
SM00695 DUSP, 2 hits
SM00290 ZnF_UBP, 1 hit
SUPFAMiSSF143791 SSF143791, 2 hits
SSF54001 SSF54001, 3 hits
PROSITEiView protein in PROSITE
PS51283 DUSP, 2 hits
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit
PS50271 ZF_UBP, 1 hit

Sequencei

Sequence statusi: Complete.

Q8C6M1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDARDLCPH LDCIGEVTKE DLLLKSKGTC QSCGVAGPNL WACLQVTCPY
60 70 80 90 100
VGCGESFADH SSIHAQVKKH NLTVNLTTFR VWCYACEREV FLEQRLAVHL
110 120 130 140 150
ASSSARLSEQ DSPPPSHPLK AVPIAVADEG ESESEDDDLK PRGLTGMKNL
160 170 180 190 200
GNSCYMNAAL QALSNCPPLT QFFLECGGLV RTDKKPALCK SYQKLISEVW
210 220 230 240 250
HKKRPSYVVP TSLSHGIKLV NPMFRGYAQQ DTQEFLRCLM DQLHEELKEP
260 270 280 290 300
MVAAVAALTD ARDSDSSDTD ERRDGDRSPS EDEFLSCDSS SDRGEGDGQG
310 320 330 340 350
RGGGSSKAEM ELLISDEAGR AISEKERMKD RKFSWGQQRT NSEQVDEDAD
360 370 380 390 400
VDTAMASLDE QSREAQPPSP RSTSPCQTPE PDNEAHIRSS SRPCSPVHHH
410 420 430 440 450
HEGHSKLSSS PPRASPVRMG PSYVLKKAQV PSTGGRRRKE QSYRSVISDV
460 470 480 490 500
FNGSVLSLVQ CLTCDRVSTT VETFQDLSLP IPGKEDLAKL HSAIYQNVPA
510 520 530 540 550
KPGACGDSYS SQGWLAFIVE YIRRFVVSCT PSWFWGPVVT LEDCLAAFFA
560 570 580 590 600
ADELKGDNMY SCERCKKLRN GVKYCKVLCL PEILCVHLKR FRHEVMYSFK
610 620 630 640 650
VSSHVSFPLE GLDLRPFLAK ECTSQVTTYD LLSVICHHGT AGSGHYIAYC
660 670 680 690 700
QNVINGQWYE FDDQYVTEVH ETVVQNVEAY VLFYRKSSEE AMRERQQVVS
710 720 730 740 750
LAAMREPSLL RFYVSREWLN KFNTFAEPGP ITNHTFLCSH GGIPPNKYHY
760 770 780 790 800
IDDLVVILPQ SVWEHLYSRF GGGPAVNHLY VCSICQVEIE ALAKRRRVEI
810 820 830 840 850
DTFIKLNKAF QAEESPAVIY CISMHWFREW EAFVKGKDSE PPGPIDNSRI
860 870 880 890 900
AQVKGSGHIQ LKQGADCGQI SEETWTYLSS LYGGGPEIAI RQSVAQLPDP
910
ESLHGEQKIE AETRAL
Length:916
Mass (Da):102,140
Last modified:March 1, 2003 - v1
Checksum:iE104BD489E7ED49F
GO

Sequence cautioni

The sequence BAD32361 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti117H → T in AAN15803 (PubMed:12056827).Curated1
Sequence conflicti380E → D in AAN15803 (PubMed:12056827).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF449715 mRNA Translation: AAN15803.1
AK173083 mRNA Translation: BAD32361.1 Different initiation.
AK054279 mRNA Translation: BAC35715.1
AK163663 mRNA Translation: BAE37447.1
AL844546 Genomic DNA Translation: CAM18433.1
CH466542 Genomic DNA Translation: EDL08500.1
BC079674 mRNA Translation: AAH79674.1
CCDSiCCDS15893.1
RefSeqiNP_083122.1, NM_028846.5
UniGeneiMm.346654

Genome annotation databases

EnsembliENSMUST00000102849; ENSMUSP00000099913; ENSMUSG00000026854
ENSMUST00000170476; ENSMUSP00000127388; ENSMUSG00000026854
GeneIDi74270
KEGGimmu:74270
UCSCiuc008jde.2 mouse

Similar proteinsi

Entry informationi

Entry nameiUBP20_MOUSE
AccessioniPrimary (citable) accession number: Q8C6M1
Secondary accession number(s): Q69ZT5, Q8CJ72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: March 1, 2003
Last modified: June 20, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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