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Protein

E3 ubiquitin-protein ligase pellino homolog 1

Gene

Peli1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (By similarity). Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation (PubMed:16951688). Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 (PubMed:29883609). Negatively regulates necroptosis by reducing RIPK3 expression (PubMed:29883609). Mediates 'Lys-63'-linked ubiquitination of RIPK1 (By similarity).By similarity2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-9020702 Interleukin-1 signaling
R-MMU-937039 IRAK1 recruits IKK complex
R-MMU-975144 IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase pellino homolog 1 (EC:2.3.2.27)
Short name:
Pellino-1
Alternative name(s):
RING-type E3 ubiquitin transferase pellino homolog 1Curated
Gene namesi
Name:Peli1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1914495 Peli1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941731 – 418E3 ubiquitin-protein ligase pellino homolog 1Add BLAST418

Post-translational modificationi

Phosphorylated by IRAK1 and IRAK4 enhancing its E3 ligase activity.By similarity
Sumoylated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8C669
PaxDbiQ8C669
PRIDEiQ8C669

PTM databases

iPTMnetiQ8C669
PhosphoSitePlusiQ8C669

Expressioni

Gene expression databases

BgeeiENSMUSG00000020134 Expressed in 286 organ(s), highest expression level in lymph node
CleanExiMM_PELI1
ExpressionAtlasiQ8C669 baseline and differential
GenevisibleiQ8C669 MM

Interactioni

Subunit structurei

Interacts with MAP3K7 (By similarity). Upon IL1B treatment, forms a complex with TRAF6, IRAK1, IRAK4 and MYD88; this complex recruits MAP3K7/TAK1, TAB1 and TAB2 to mediate NF-kappa-B activation. Direct binding of SMAD6 to PELI1 prevents the complex formation and hence negatively regulates IL1R-TLR signaling and eventually NF-kappa-B-mediated gene expression (PubMed:16951688). Interacts (via atypical FHA domain) with RIPK3 (PubMed:29883609). Binds preferentially to the 'Thr-182' phosphorylated form of RIPK3 (By similarity). Interacts with RIPK1 (PubMed:29883609).By similarity2 Publications

Protein-protein interaction databases

BioGridi212043, 3 interactors
DIPiDIP-48336N
IntActiQ8C669, 2 interactors
STRINGi10090.ENSMUSP00000090979

Structurei

3D structure databases

ProteinModelPortaliQ8C669
SMRiQ8C669
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 200FHA; atypicalBy similarityAdd BLAST188

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni311 – 399Ring-like domain; necessary for ubiquitination of RIPK3By similarityAdd BLAST89

Sequence similaritiesi

Belongs to the pellino family.Curated

Phylogenomic databases

eggNOGiKOG3842 Eukaryota
ENOG410XP3S LUCA
GeneTreeiENSGT00390000007680
HOGENOMiHOG000234110
HOVERGENiHBG053559
InParanoidiQ8C669
KOiK11964
OMAiCPFCAQQ
OrthoDBiEOG091G0631
PhylomeDBiQ8C669
TreeFamiTF314338

Family and domain databases

InterProiView protein in InterPro
IPR006800 Pellino_fam
PANTHERiPTHR12098 PTHR12098, 1 hit
PfamiView protein in Pfam
PF04710 Pellino, 1 hit
PIRSFiPIRSF038886 Pellino, 1 hit

Sequencei

Sequence statusi: Complete.

Q8C669-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFSPDQENHP SKAPVKYGEL IVLGYNGSLP NGDRGRRKSR FALFKRPKAN
60 70 80 90 100
GVKPSTVHIA CTPQAAKAIS NKDQHSISYT LSRAQTVVVE YTHDSNTDMF
110 120 130 140 150
QIGRSTESPI DFVVTDTVPG SQSNSDTQSV QSTISRFACR IICERSPPFT
160 170 180 190 200
ARIYAAGFDS SKNIFLGEKA AKWKTSDGQM DGLTTNGVLV MHPRNGFTED
210 220 230 240 250
SKPGIWREIS VCGNVFSLRE TRSAQQRGKM VEIETNQLQD GSLIDLCGAT
260 270 280 290 300
LLWRTAEGLS HTPTVKHLEA LRQEINAARP QCPVGFNTLA FPSMKRKDVV
310 320 330 340 350
DEKQPWVYLN CGHVHGYHNW GNKEERDGKD RECPMCRSVG PYVPLWLGCE
360 370 380 390 400
AGFYVDAGPP THAFSPCGHV CSEKTTAYWS QIPLPHGTHT FHAACPFCAH
410
QLAGEQGYIR LIFQGPLD
Length:418
Mass (Da):46,259
Last modified:October 24, 2003 - v2
Checksum:i26072654577EBBF7
GO

Sequence cautioni

The sequence BAC36351 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5 – 7DQE → GSR in AAG15391 (PubMed:11306823).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302503 mRNA Translation: AAG15391.1
AK009945 mRNA Translation: BAB26600.1
AK045673 mRNA Translation: BAC32452.1
AK076458 mRNA Translation: BAC36351.1 Different initiation.
BC016515 mRNA Translation: AAH16515.1
CCDSiCCDS24462.1
RefSeqiNP_075813.2, NM_023324.2
UniGeneiMm.28957

Genome annotation databases

EnsembliENSMUST00000093290; ENSMUSP00000090979; ENSMUSG00000020134
ENSMUST00000101477; ENSMUSP00000099018; ENSMUSG00000020134
GeneIDi67245
KEGGimmu:67245
UCSCiuc007idl.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302503 mRNA Translation: AAG15391.1
AK009945 mRNA Translation: BAB26600.1
AK045673 mRNA Translation: BAC32452.1
AK076458 mRNA Translation: BAC36351.1 Different initiation.
BC016515 mRNA Translation: AAH16515.1
CCDSiCCDS24462.1
RefSeqiNP_075813.2, NM_023324.2
UniGeneiMm.28957

3D structure databases

ProteinModelPortaliQ8C669
SMRiQ8C669
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212043, 3 interactors
DIPiDIP-48336N
IntActiQ8C669, 2 interactors
STRINGi10090.ENSMUSP00000090979

PTM databases

iPTMnetiQ8C669
PhosphoSitePlusiQ8C669

Proteomic databases

MaxQBiQ8C669
PaxDbiQ8C669
PRIDEiQ8C669

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000093290; ENSMUSP00000090979; ENSMUSG00000020134
ENSMUST00000101477; ENSMUSP00000099018; ENSMUSG00000020134
GeneIDi67245
KEGGimmu:67245
UCSCiuc007idl.1 mouse

Organism-specific databases

CTDi57162
MGIiMGI:1914495 Peli1

Phylogenomic databases

eggNOGiKOG3842 Eukaryota
ENOG410XP3S LUCA
GeneTreeiENSGT00390000007680
HOGENOMiHOG000234110
HOVERGENiHBG053559
InParanoidiQ8C669
KOiK11964
OMAiCPFCAQQ
OrthoDBiEOG091G0631
PhylomeDBiQ8C669
TreeFamiTF314338

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-9020702 Interleukin-1 signaling
R-MMU-937039 IRAK1 recruits IKK complex
R-MMU-975144 IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation

Miscellaneous databases

ChiTaRSiPeli1 mouse
PROiPR:Q8C669
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020134 Expressed in 286 organ(s), highest expression level in lymph node
CleanExiMM_PELI1
ExpressionAtlasiQ8C669 baseline and differential
GenevisibleiQ8C669 MM

Family and domain databases

InterProiView protein in InterPro
IPR006800 Pellino_fam
PANTHERiPTHR12098 PTHR12098, 1 hit
PfamiView protein in Pfam
PF04710 Pellino, 1 hit
PIRSFiPIRSF038886 Pellino, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPELI1_MOUSE
AccessioniPrimary (citable) accession number: Q8C669
Secondary accession number(s): Q91YL9, Q9CV22, Q9ERJ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: October 24, 2003
Last modified: November 7, 2018
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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