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Protein

CCR4-NOT transcription complex subunit 2

Gene

Cnot2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for the CCR4-NOT complex structural integrity. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may specifically involve the N-Cor repressor complex containing HDAC3, NCOR1 and NCOR2. Involved in the maintenance of emryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Repressor
Biological processRNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcription complex subunit 2
Alternative name(s):
CCR4-associated factor 2
Gene namesi
Name:Cnot2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1919318 Cnot2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001983321 – 540CCR4-NOT transcription complex subunit 2Add BLAST540

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei93PhosphothreonineBy similarity1
Modified residuei126PhosphoserineBy similarity1
Modified residuei157PhosphoserineCombined sources1
Modified residuei165PhosphoserineCombined sources1
Modified residuei169PhosphoserineCombined sources1
Modified residuei242PhosphoserineBy similarity1
Modified residuei274PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8C5L3
PRIDEiQ8C5L3

PTM databases

iPTMnetiQ8C5L3
PhosphoSitePlusiQ8C5L3

Expressioni

Developmental stagei

Expressed in embryonic stem (ES) cells and in inner cell mass (ICM) of the blastocyst.1 Publication

Gene expression databases

BgeeiENSMUSG00000020166 Expressed in 290 organ(s), highest expression level in skin of back
CleanExiMM_CNOT2
ExpressionAtlasiQ8C5L3 baseline and differential
GenevisibleiQ8C5L3 MM

Interactioni

Subunit structurei

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT3. Interacts with NCOR1, NCOR2. HDAC3 and GPS2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi215128, 19 interactors
IntActiQ8C5L3, 19 interactors
STRINGi10090.ENSMUSP00000100902

Structurei

3D structure databases

ProteinModelPortaliQ8C5L3
SMRiQ8C5L3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CNOT2/3/5 family.Curated

Phylogenomic databases

eggNOGiKOG2151 Eukaryota
COG5601 LUCA
GeneTreeiENSGT00390000001285
HOGENOMiHOG000246523
HOVERGENiHBG051041
InParanoidiQ8C5L3
KOiK12605
OrthoDBiEOG091G0C7Y
PhylomeDBiQ8C5L3
TreeFamiTF313102

Family and domain databases

Gene3Di2.30.30.1020, 1 hit
InterProiView protein in InterPro
IPR038635 CCR4-NOT_su2/3/5_N_sf
IPR007282 NOT2/3/5_C
PfamiView protein in Pfam
PF04153 NOT2_3_5, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8C5L3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVRTDGHTLS EKRNYQVTNS MFGASRKKFV EGVDSDYHDE NMYYSQSSMF
60 70 80 90 100
PHRSEKDMLA SPSTSGQLSQ FGASLYGQQS ALGLPMRGMS NNTPQLNRSL
110 120 130 140 150
SQGTQLPSHV TPTTGVPTMS LHTPPSPSRG ILPMNPRNMM NHSQVGQGIG
160 170 180 190 200
IPSRTNSMSS SGLGSPNRSS PSIICMPKQQ PSRQPFTVNS MSGFGMNRNQ
210 220 230 240 250
AFGMNNSLSS NIFNGTDGSE NVTGLDLSDF PALADRNRRE GSGNPTPLIN
260 270 280 290 300
PLAGRAPYVG MVTKPANEQS QDFSIHNEDF PALPGSSYKD PTSSNDDSKS
310 320 330 340 350
NLSTSGKTTS STDGPKFPGD KSSTTQNNNQ QKKGIQVLPD GRVTNIPQGM
360 370 380 390 400
VTDQFGMIGL LTFIRAAETD PGMVHLALGS DLTTLGLNLN SPENLYPKFA
410 420 430 440 450
SPWASSPCRP QDIDFHVPSE YLTNIHIRDK LAAIKLGRYG EDLLFYLYYM
460 470 480 490 500
NGGDVLQLLA AVELFNRDWR YHKEERVWIT RAPGMEPTMK TNTYERGTYY
510 520 530 540
FFDCLNWRKV AKEFHLEYDK LEERPHLPST FNYNPAQQAF
Length:540
Mass (Da):59,711
Last modified:April 13, 2004 - v2
Checksum:iC5185262B5E3C78C
GO
Isoform 2 (identifier: Q8C5L3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MVRTDGHTLSEKRNY → MLKEVA

Note: No experimental confirmation available.
Show »
Length:531
Mass (Da):58,594
Checksum:i242DC8CF90C700E6
GO
Isoform 3 (identifier: Q8C5L3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.

Note: No experimental confirmation available.
Show »
Length:455
Mass (Da):50,224
Checksum:i9778467D3A90FB8E
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q7M5E9Q7M5_MOUSE
CCR4-NOT transcription complex subu...
Cnot2
48Annotation score:
E9Q8D5E9Q8D5_MOUSE
CCR4-NOT transcription complex subu...
Cnot2
490Annotation score:
E9Q027E9Q027_MOUSE
CCR4-NOT transcription complex subu...
Cnot2
499Annotation score:
H7BWX6H7BWX6_MOUSE
CCR4-NOT transcription complex subu...
Cnot2
109Annotation score:
A0A1W2P771A0A1W2P771_MOUSE
CCR4-NOT transcription complex subu...
Cnot2
78Annotation score:
E9Q8R6E9Q8R6_MOUSE
CCR4-NOT transcription complex subu...
Cnot2
57Annotation score:

Sequence cautioni

The sequence AAH43133 differs from that shown. Reason: Frameshift at position 1.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti148G → S in BAC37134 (PubMed:16141072).Curated1
Sequence conflicti426H → Y in AAH43133 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0099171 – 85Missing in isoform 3. 1 PublicationAdd BLAST85
Alternative sequenceiVSP_0099181 – 15MVRTD…EKRNY → MLKEVA in isoform 2. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011231 mRNA Translation: BAB27481.1
AK078121 mRNA Translation: BAC37134.1
AK149767 mRNA Translation: BAE29072.1
BC043133 mRNA Translation: AAH43133.1 Frameshift.
BC063105 mRNA Translation: AAH63105.1
BC065171 mRNA Translation: AAH65171.1
CCDSiCCDS36064.1 [Q8C5L3-1]
RefSeqiNP_001032935.2, NM_001037846.3 [Q8C5L3-1]
NP_001032936.2, NM_001037847.2
NP_001032937.1, NM_001037848.3
NP_082358.2, NM_028082.2
XP_006514238.1, XM_006514175.3 [Q8C5L3-1]
XP_006514240.1, XM_006514177.3
XP_006514242.1, XM_006514179.3
XP_006514243.1, XM_006514180.1
XP_006514244.1, XM_006514181.1 [Q8C5L3-2]
XP_006514246.1, XM_006514183.3 [Q8C5L3-3]
XP_006514247.1, XM_006514184.3 [Q8C5L3-3]
XP_017169595.1, XM_017314106.1
XP_017169596.1, XM_017314107.1 [Q8C5L3-2]
XP_017169597.1, XM_017314108.1
XP_017169598.1, XM_017314109.1
UniGeneiMm.351553

Genome annotation databases

EnsembliENSMUST00000105265; ENSMUSP00000100900; ENSMUSG00000020166 [Q8C5L3-3]
ENSMUST00000105267; ENSMUSP00000100902; ENSMUSG00000020166 [Q8C5L3-1]
ENSMUST00000169921; ENSMUSP00000132152; ENSMUSG00000020166 [Q8C5L3-1]
GeneIDi72068
KEGGimmu:72068
UCSCiuc007hcb.2 mouse [Q8C5L3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011231 mRNA Translation: BAB27481.1
AK078121 mRNA Translation: BAC37134.1
AK149767 mRNA Translation: BAE29072.1
BC043133 mRNA Translation: AAH43133.1 Frameshift.
BC063105 mRNA Translation: AAH63105.1
BC065171 mRNA Translation: AAH65171.1
CCDSiCCDS36064.1 [Q8C5L3-1]
RefSeqiNP_001032935.2, NM_001037846.3 [Q8C5L3-1]
NP_001032936.2, NM_001037847.2
NP_001032937.1, NM_001037848.3
NP_082358.2, NM_028082.2
XP_006514238.1, XM_006514175.3 [Q8C5L3-1]
XP_006514240.1, XM_006514177.3
XP_006514242.1, XM_006514179.3
XP_006514243.1, XM_006514180.1
XP_006514244.1, XM_006514181.1 [Q8C5L3-2]
XP_006514246.1, XM_006514183.3 [Q8C5L3-3]
XP_006514247.1, XM_006514184.3 [Q8C5L3-3]
XP_017169595.1, XM_017314106.1
XP_017169596.1, XM_017314107.1 [Q8C5L3-2]
XP_017169597.1, XM_017314108.1
XP_017169598.1, XM_017314109.1
UniGeneiMm.351553

3D structure databases

ProteinModelPortaliQ8C5L3
SMRiQ8C5L3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215128, 19 interactors
IntActiQ8C5L3, 19 interactors
STRINGi10090.ENSMUSP00000100902

PTM databases

iPTMnetiQ8C5L3
PhosphoSitePlusiQ8C5L3

Proteomic databases

PaxDbiQ8C5L3
PRIDEiQ8C5L3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000105265; ENSMUSP00000100900; ENSMUSG00000020166 [Q8C5L3-3]
ENSMUST00000105267; ENSMUSP00000100902; ENSMUSG00000020166 [Q8C5L3-1]
ENSMUST00000169921; ENSMUSP00000132152; ENSMUSG00000020166 [Q8C5L3-1]
GeneIDi72068
KEGGimmu:72068
UCSCiuc007hcb.2 mouse [Q8C5L3-1]

Organism-specific databases

CTDi4848
MGIiMGI:1919318 Cnot2

Phylogenomic databases

eggNOGiKOG2151 Eukaryota
COG5601 LUCA
GeneTreeiENSGT00390000001285
HOGENOMiHOG000246523
HOVERGENiHBG051041
InParanoidiQ8C5L3
KOiK12605
OrthoDBiEOG091G0C7Y
PhylomeDBiQ8C5L3
TreeFamiTF313102

Enzyme and pathway databases

ReactomeiR-MMU-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain

Miscellaneous databases

ChiTaRSiCnot2 mouse
PROiPR:Q8C5L3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020166 Expressed in 290 organ(s), highest expression level in skin of back
CleanExiMM_CNOT2
ExpressionAtlasiQ8C5L3 baseline and differential
GenevisibleiQ8C5L3 MM

Family and domain databases

Gene3Di2.30.30.1020, 1 hit
InterProiView protein in InterPro
IPR038635 CCR4-NOT_su2/3/5_N_sf
IPR007282 NOT2/3/5_C
PfamiView protein in Pfam
PF04153 NOT2_3_5, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCNOT2_MOUSE
AccessioniPrimary (citable) accession number: Q8C5L3
Secondary accession number(s): Q3UE39, Q80YA5, Q9D0P1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: November 7, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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