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UniProtKB - Q8C5D8 (PIAS2_MOUSE)

Entry version 162 (02 Dec 2020)
Sequence version 2 (07 Dec 2004)
Previous versions | rss
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Protein

E3 SUMO-protein ligase PIAS2

Gene

Pias2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIASx-beta, but not isoform PIASx-alpha, promotes MDM2 sumoylation. Isoform PIASx-alpha promotes PARK7 sumoylation. Isoform PIASx-beta promotes NCOA2 sumoylation more efficiently than isoform PIASx-alpha (By similarity). Sumoylates PML at'Lys-65' and 'Lys-160' (By similarity).By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri331 – 408SP-RING-typePROSITE-ProRule annotationAdd BLAST78

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Transferase
Biological processTranscription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-MMU-3108214, SUMOylation of DNA damage response and repair proteins
R-MMU-3232118, SUMOylation of transcription factors
R-MMU-3232142, SUMOylation of ubiquitinylation proteins
R-MMU-3899300, SUMOylation of transcription cofactors
R-MMU-4090294, SUMOylation of intracellular receptors
R-MMU-4551638, SUMOylation of chromatin organization proteins

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00886

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
E3 SUMO-protein ligase PIAS2 (EC:2.3.2.27)
Alternative name(s):
Androgen receptor-interacting protein 3
Short name:
ARIP3
DAB2-interacting protein
Short name:
DIP
Msx-interacting zinc finger protein
Protein inhibitor of activated STAT x
Protein inhibitor of activated STAT2
RING-type E3 ubiquitin transferase PIAS2Curated
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Pias2
Synonyms:Miz1, Piasx
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 18

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...MGIi		MGI:1096566, Pias2

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002189771 – 621E3 SUMO-protein ligase PIAS2Add BLAST621

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki249Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki430Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki435Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki443Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki452Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei476PhosphoserineBy similarity1
Modified residuei477PhosphoserineBy similarity1
Modified residuei478PhosphoserineBy similarity1
Cross-linki489Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei499PhosphoserineCombined sources1
Cross-linki502Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Sumoylated.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q8C5D8

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q8C5D8

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q8C5D8

PRoteomics IDEntifications database

More...PRIDEi		Q8C5D8

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q8C5D8

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q8C5D8

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSMUSG00000025423, Expressed in testis and 314 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q8C5D8, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q8C5D8, MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Binds SUMO1 and UBE2I.

Interacts with AXIN1, JUN, MDM2, PARK7, TP53 and TP73 isoform alpha, but not TP73 isoform beta.

Interacts with STAT4 following IL12 and IFN-alpha stimulation of T-cells.

Interacts also with GTF2I, GTF2IRD1, IKFZ1, DAB2 and MSX2, as well as with several steroid receptors, including ESR1, ESR2, NR3C1, PGR, AR, and with NCOA2. Sumoylation of a target protein seems to enhance the interaction. Binds to sumoylated ELK1.

Interacts with PLAG1 (By similarity). Binds DNA, such as CDKN1A promoter, in a sequence-specific manner.

Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G1 phase (By similarity).

Interacts with IFIH1/MDA5 (By similarity).

Interacts with PML (By similarity).

Interacts with PRDM1 (By similarity).

By similarity

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		201429, 14 interactors

Protein interaction database and analysis system

More...IntActi		Q8C5D8, 6 interactors

Molecular INTeraction database

More...MINTi		Q8C5D8

STRING: functional protein association networks

More...STRINGi		10090.ENSMUSP00000110425

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q8C5D8, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q8C5D8

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini11 – 45SAPPROSITE-ProRule annotationAdd BLAST35
Domaini134 – 299PINITPROSITE-ProRule annotationAdd BLAST166

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni467 – 473SUMO1-bindingBy similarity7

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi19 – 23LXXLL motif5
Motifi484 – 492Nuclear localization signal1 Publication9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi571 – 610Ser-richAdd BLAST40
Compositional biasi596 – 602Poly-Ser7

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The LXXLL motif is a transcriptional coregulator signature.

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the PIAS family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri331 – 408SP-RING-typePROSITE-ProRule annotationAdd BLAST78

Keywords - Domaini

Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG2169, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00990000203531

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q8C5D8

Identification of Orthologs from Complete Genome Data

More...OMAi		DPQQYCP

Database of Orthologous Groups

More...OrthoDBi		1205949at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q8C5D8

TreeFam database of animal gene trees

More...TreeFami		TF323787

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.720.30, 1 hit
2.60.120.780, 1 hit
3.30.40.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR027228, PIAS2
IPR023321, PINIT
IPR038654, PINIT_sf
IPR003034, SAP_dom
IPR036361, SAP_dom_sf
IPR004181, Znf_MIZ
IPR013083, Znf_RING/FYVE/PHD

The PANTHER Classification System

More...PANTHERi		PTHR10782:SF12, PTHR10782:SF12, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF14324, PINIT, 1 hit
PF02891, zf-MIZ, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00513, SAP, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF68906, SSF68906, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51466, PINIT, 1 hit
PS50800, SAP, 1 hit
PS51044, ZF_SP_RING, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (5+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 5 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8C5D8-1) [UniParc]FASTAAdd to basket
Also known as: PIASx-beta, Miz1

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MADFEELRNM VSSFRVSELQ VLLGFAGRNK SGRKHDLLMR ALHLLKSGCS 
        60         70         80         90        100
PAVQIKIREL YRRRYPRTLE GLCDLSTIKS SVFSLDGSSS PVEPDLPVAG 
       110        120        130        140        150
IHSLPSTSIT PHSPSSPVGS VLLQDTKPTF EMQQPSPPIP PVHPDVQLKN 
       160        170        180        190        200
LPFYDVLDVL IKPTSLVQSS IQRFQEKFFI FALTPQQVRE ICISRDFLPG 
       210        220        230        240        250
GRRDYTVQVQ LRLCLAETSC PQEDNYPNSL CIKVNGKLFP LPGYAPPPKN 
       260        270        280        290        300
GIEQKRPGRP LNITSLVRLS SAVPNQISIS WASEIGKNYS MSVYLVRQLT 
       310        320        330        340        350
SAMLLQRLKM KGIRNPDHSR ALIKEKLTAD PDSEIATTSL RVSLMCPLGK 
       360        370        380        390        400
MRLTIPCRAV TCTHLQCFDA ALYLQMNEKK PTWICPVCDK KAAYESLILD 
       410        420        430        440        450
GLFMEILNDC SDVDEIKFQE DGSWCPMRPK KEAMKVTSQP CTKVESSSVF 
       460        470        480        490        500
SKPCSVTVAS DASKKKIDVI DLTIESSSDE EEDPPAKRKC IFMSETQSSP 
       510        520        530        540        550
TKGVLMYQPS SVRVPSVTSV DPAAIPPSLT DYSVPFHHTP VSSMSSDLPG 
       560        570        580        590        600
LDFLSLIPVD PQYCPPMFLD SLTSPLTASS TSVTTTSPHE SSTHVSSSSS 
       610        620 
RSETGVITSS GRNIPDIISL D
Length:621
Mass (Da):68,426
Last modified:December 7, 2004 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE980B834C2A9C58E
GO
Isoform 2 (identifier: Q8C5D8-2) [UniParc]FASTAAdd to basket
Also known as: PIASx-alpha, ARIP3

The sequence of this isoform differs from the canonical sequence as follows:
     551-572: LDFLSLIPVDPQYCPPMFLDSL → EQRRNDINNEVQLGASSDTVQQ
     573-621: Missing.

Show »
Length:572
Mass (Da):63,458
Checksum:i70668C1CEB3702EE
GO
Isoform 3 (identifier: Q8C5D8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.
     551-572: LDFLSLIPVDPQYCPPMFLDSL → EQRRNDINNEVQLGASSDTVQQ
     573-621: Missing.

Show »
Length:563
Mass (Da):62,352
Checksum:i25D6D636E852AA07
GO
Isoform 4 (identifier: Q8C5D8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     563-580: YCPPMFLDSLTSPLTASS → SHLTLNSKQYVCHHHQPP
     581-621: Missing.

Show »
Length:580
Mass (Da):64,421
Checksum:i4461E5438EC68633
GO
Isoform 5 (identifier: Q8C5D8-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Show »
Length:612
Mass (Da):67,320
Checksum:i98EA1B83556CDE86
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WHS8F8WHS8_MOUSE
E3 SUMO-protein ligase PIAS2
Pias2
565Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
G3UWE3G3UWE3_MOUSE
E3 SUMO-protein ligase PIAS2
Pias2
614Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A494B929A0A494B929_MOUSE
E3 SUMO-protein ligase PIAS2
Pias2
252Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAB96678 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB29594 differs from that shown. Reason: Frameshift.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti73C → S in AAF12825 (PubMed:11104669).Curated1
Sequence conflicti97P → A in AAF12825 (PubMed:11104669).Curated1
Sequence conflicti110T → A in AAF12825 (PubMed:11104669).Curated1
Sequence conflicti119G → E in AAF12825 (PubMed:11104669).Curated1
Sequence conflicti150N → T in AAF12825 (PubMed:11104669).Curated1
Sequence conflicti158D → E in AAF12825 (PubMed:11104669).Curated1
Sequence conflicti173R → G in BAC26579 (PubMed:16141072).Curated1
Sequence conflicti243G → A in AAF12825 (PubMed:11104669).Curated1
Sequence conflicti288N → Q in AAF12825 (PubMed:11104669).Curated1
Sequence conflicti320R → K in AAF12825 (PubMed:11104669).Curated1
Sequence conflicti327L → P in AAF12825 (PubMed:11104669).Curated1
Sequence conflicti375Q → R in AAF12825 (PubMed:11104669).Curated1
Sequence conflicti413V → A in AAF12825 (PubMed:11104669).Curated1
Sequence conflicti438S → I in BAC37407 (PubMed:16141072).Curated1
Sequence conflicti458V → G in AAF12825 (PubMed:11104669).Curated1
Sequence conflicti464 – 466KKK → GTR in AAF12825 (PubMed:11104669).Curated3
Isoform 2 (identifier: Q8C5D8-2)
Sequence conflicti555N → T in AAF12825 (PubMed:11104669).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0121981 – 9Missing in isoform 3 and isoform 5. 1 Publication9
Alternative sequenceiVSP_012199551 – 572LDFLS…FLDSL → EQRRNDINNEVQLGASSDTV QQ in isoform 2 and isoform 3. 3 PublicationsAdd BLAST22
Alternative sequenceiVSP_012200563 – 580YCPPM…LTASS → SHLTLNSKQYVCHHHQPP in isoform 4. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_012201573 – 621Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST49
Alternative sequenceiVSP_012202581 – 621Missing in isoform 4. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AF201391 mRNA Translation: AAF12825.1
AK029716 mRNA Translation: BAC26579.1
AK014871 mRNA Translation: BAB29594.1 Frameshift.
AK078813 mRNA Translation: BAC37407.1
AK086653 mRNA Translation: BAC39710.1
BC005596 mRNA Translation: AAH05596.1
BC034711 mRNA Translation: AAH34711.1
AF039567 mRNA Translation: AAB96678.1 Different initiation.

The Consensus CDS (CCDS) project

More...CCDSi		CCDS37866.1 [Q8C5D8-1]

NCBI Reference Sequences

More...RefSeqi		NP_001157639.1, NM_001164167.1 [Q8C5D8-4]
NP_001157640.1, NM_001164168.1
NP_001157641.1, NM_001164169.1 [Q8C5D8-2]
NP_001157642.1, NM_001164170.1
NP_032628.3, NM_008602.4 [Q8C5D8-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENSMUST00000114777; ENSMUSP00000110425; ENSMUSG00000025423 [Q8C5D8-1]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		17344

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		mmu:17344

UCSC genome browser

More...UCSCi		uc008frb.1, mouse [Q8C5D8-2]
uc008frc.2, mouse [Q8C5D8-1]
uc012bfb.1, mouse [Q8C5D8-4]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF201391 mRNA Translation: AAF12825.1
AK029716 mRNA Translation: BAC26579.1
AK014871 mRNA Translation: BAB29594.1 Frameshift.
AK078813 mRNA Translation: BAC37407.1
AK086653 mRNA Translation: BAC39710.1
BC005596 mRNA Translation: AAH05596.1
BC034711 mRNA Translation: AAH34711.1
AF039567 mRNA Translation: AAB96678.1 Different initiation.
CCDSiCCDS37866.1 [Q8C5D8-1]
RefSeqiNP_001157639.1, NM_001164167.1 [Q8C5D8-4]
NP_001157640.1, NM_001164168.1
NP_001157641.1, NM_001164169.1 [Q8C5D8-2]
NP_001157642.1, NM_001164170.1
NP_032628.3, NM_008602.4 [Q8C5D8-1]

3D structure databases

SMRiQ8C5D8
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi201429, 14 interactors
IntActiQ8C5D8, 6 interactors
MINTiQ8C5D8
STRINGi10090.ENSMUSP00000110425

PTM databases

iPTMnetiQ8C5D8
PhosphoSitePlusiQ8C5D8

Proteomic databases

EPDiQ8C5D8
jPOSTiQ8C5D8
PaxDbiQ8C5D8
PRIDEiQ8C5D8

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		9110, 506 antibodies

Genome annotation databases

EnsembliENSMUST00000114777; ENSMUSP00000110425; ENSMUSG00000025423 [Q8C5D8-1]
GeneIDi17344
KEGGimmu:17344
UCSCiuc008frb.1, mouse [Q8C5D8-2]
uc008frc.2, mouse [Q8C5D8-1]
uc012bfb.1, mouse [Q8C5D8-4]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		9063
MGIiMGI:1096566, Pias2

Phylogenomic databases

eggNOGiKOG2169, Eukaryota
GeneTreeiENSGT00990000203531
InParanoidiQ8C5D8
OMAiDPQQYCP
OrthoDBi1205949at2759
PhylomeDBiQ8C5D8
TreeFamiTF323787

Enzyme and pathway databases

UniPathwayiUPA00886
ReactomeiR-MMU-3108214, SUMOylation of DNA damage response and repair proteins
R-MMU-3232118, SUMOylation of transcription factors
R-MMU-3232142, SUMOylation of ubiquitinylation proteins
R-MMU-3899300, SUMOylation of transcription cofactors
R-MMU-4090294, SUMOylation of intracellular receptors
R-MMU-4551638, SUMOylation of chromatin organization proteins

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		17344, 3 hits in 17 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		Pias2, mouse

Protein Ontology

More...PROi		PR:Q8C5D8
RNActiQ8C5D8, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSMUSG00000025423, Expressed in testis and 314 other tissues
ExpressionAtlasiQ8C5D8, baseline and differential
GenevisibleiQ8C5D8, MM

Family and domain databases

Gene3Di1.10.720.30, 1 hit
2.60.120.780, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR027228, PIAS2
IPR023321, PINIT
IPR038654, PINIT_sf
IPR003034, SAP_dom
IPR036361, SAP_dom_sf
IPR004181, Znf_MIZ
IPR013083, Znf_RING/FYVE/PHD
PANTHERiPTHR10782:SF12, PTHR10782:SF12, 1 hit
PfamiView protein in Pfam
PF14324, PINIT, 1 hit
PF02891, zf-MIZ, 1 hit
SMARTiView protein in SMART
SM00513, SAP, 1 hit
SUPFAMiSSF68906, SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS51466, PINIT, 1 hit
PS50800, SAP, 1 hit
PS51044, ZF_SP_RING, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPIAS2_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8C5D8
Secondary accession number(s): O54987
, Q8C384, Q8CDQ8, Q8K208, Q99JX5, Q9D5W7, Q9QZ63
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: December 2, 2020
This is version 162 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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