UniProtKB - Q8C5D8 (PIAS2_MOUSE)
E3 SUMO-protein ligase PIAS2
Pias2
Functioni
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein sumoylation Pathwayi
This protein is involved in the pathway protein sumoylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 331 – 408 | SP-RING-typePROSITE-ProRule annotationAdd BLAST | 78 |
GO - Molecular functioni
- androgen receptor binding Source: MGI
- DNA binding Source: MGI
- estrogen receptor binding Source: MGI
- glucocorticoid receptor binding Source: MGI
- protein domain specific binding Source: MGI
- RNA polymerase II transcription factor binding Source: GO_Central
- SUMO ligase activity Source: ParkinsonsUK-UCL
- SUMO transferase activity Source: MGI
- transcription coregulator activity Source: GO_Central
- transcription factor binding Source: ParkinsonsUK-UCL
- ubiquitin protein ligase binding Source: BHF-UCL
- zinc ion binding Source: InterPro
GO - Biological processi
- androgen receptor signaling pathway Source: MGI
- negative regulation of androgen receptor signaling pathway Source: MGI
- negative regulation of DNA-binding transcription factor activity Source: MGI
- positive regulation of dendrite morphogenesis Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- protein sumoylation Source: MGI
- regulation of androgen receptor signaling pathway Source: MGI
- regulation of osteoblast differentiation Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- transcription, DNA-templated Source: MGI
Keywordsi
Molecular function | DNA-binding, Transferase |
Biological process | Transcription, Transcription regulation, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-3108214, SUMOylation of DNA damage response and repair proteins R-MMU-3232118, SUMOylation of transcription factors R-MMU-3232142, SUMOylation of ubiquitinylation proteins R-MMU-3899300, SUMOylation of transcription cofactors R-MMU-4090294, SUMOylation of intracellular receptors R-MMU-4551638, SUMOylation of chromatin organization proteins |
UniPathwayi | UPA00886 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 SUMO-protein ligase PIAS2 (EC:2.3.2.27)Alternative name(s): Androgen receptor-interacting protein 3 Short name: ARIP3 DAB2-interacting protein Short name: DIP Msx-interacting zinc finger protein Protein inhibitor of activated STAT x Protein inhibitor of activated STAT2 RING-type E3 ubiquitin transferase PIAS2Curated |
Gene namesi | Name:Pias2 Synonyms:Miz1, Piasx |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1096566, Pias2 |
Subcellular locationi
Nucleus
- Nucleus speckle 1 Publication
- PML body By similarity
- Nucleus 1 Publication
Note: Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) (By similarity). Colocalizes with SUMO1 in nuclear granules (PubMed:12077349).By similarity1 Publication
Nucleus
- nuclear body Source: MGI
- nuclear speck Source: UniProtKB-SubCell
- nucleoplasm Source: MGI
- nucleus Source: MGI
- PML body Source: UniProtKB-SubCell
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218977 | 1 – 621 | E3 SUMO-protein ligase PIAS2Add BLAST | 621 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 46 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 249 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 430 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 435 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 443 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 452 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 476 | PhosphoserineBy similarity | 1 | |
Modified residuei | 477 | PhosphoserineBy similarity | 1 | |
Modified residuei | 478 | PhosphoserineBy similarity | 1 | |
Cross-linki | 489 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 499 | PhosphoserineCombined sources | 1 | |
Cross-linki | 502 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8C5D8 |
jPOSTi | Q8C5D8 |
PaxDbi | Q8C5D8 |
PRIDEi | Q8C5D8 |
PTM databases
iPTMneti | Q8C5D8 |
PhosphoSitePlusi | Q8C5D8 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000025423, Expressed in testis and 314 other tissues |
ExpressionAtlasi | Q8C5D8, baseline and differential |
Genevisiblei | Q8C5D8, MM |
Interactioni
Subunit structurei
Binds SUMO1 and UBE2I.
Interacts with AXIN1, JUN, MDM2, PARK7, TP53 and TP73 isoform alpha, but not TP73 isoform beta.
Interacts with STAT4 following IL12 and IFN-alpha stimulation of T-cells.
Interacts also with GTF2I, GTF2IRD1, IKFZ1, DAB2 and MSX2, as well as with several steroid receptors, including ESR1, ESR2, NR3C1, PGR, AR, and with NCOA2. Sumoylation of a target protein seems to enhance the interaction. Binds to sumoylated ELK1.
Interacts with PLAG1 (By similarity). Binds DNA, such as CDKN1A promoter, in a sequence-specific manner.
Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G1 phase (By similarity).
Interacts with IFIH1/MDA5 (By similarity).
Interacts with PML (By similarity).
Interacts with PRDM1 (By similarity).
By similarityBinary interactionsi
Hide detailsQ8C5D8
With | #Exp. | IntAct |
---|---|---|
Dab2 [P98078] | 2 | EBI-6305825,EBI-1391846 |
Dab2 - isoform p67 [P98078-3] | 3 | EBI-6305825,EBI-6305891 |
Isoform 1 [Q8C5D8-1]
With | #Exp. | IntAct |
---|---|---|
Egr2 [P08152] | 5 | EBI-8064899,EBI-7070449 |
GO - Molecular functioni
- androgen receptor binding Source: MGI
- estrogen receptor binding Source: MGI
- glucocorticoid receptor binding Source: MGI
- protein domain specific binding Source: MGI
- RNA polymerase II transcription factor binding Source: GO_Central
- transcription factor binding Source: ParkinsonsUK-UCL
- ubiquitin protein ligase binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 201429, 14 interactors |
IntActi | Q8C5D8, 6 interactors |
MINTi | Q8C5D8 |
STRINGi | 10090.ENSMUSP00000110425 |
Miscellaneous databases
RNActi | Q8C5D8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 45 | SAPPROSITE-ProRule annotationAdd BLAST | 35 | |
Domaini | 134 – 299 | PINITPROSITE-ProRule annotationAdd BLAST | 166 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 467 – 473 | SUMO1-bindingBy similarity | 7 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 19 – 23 | LXXLL motif | 5 | |
Motifi | 484 – 492 | Nuclear localization signal1 Publication | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 571 – 610 | Ser-richAdd BLAST | 40 | |
Compositional biasi | 596 – 602 | Poly-Ser | 7 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 331 – 408 | SP-RING-typePROSITE-ProRule annotationAdd BLAST | 78 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2169, Eukaryota |
GeneTreei | ENSGT00990000203531 |
InParanoidi | Q8C5D8 |
OMAi | DPQQYCP |
OrthoDBi | 1205949at2759 |
PhylomeDBi | Q8C5D8 |
TreeFami | TF323787 |
Family and domain databases
Gene3Di | 1.10.720.30, 1 hit 2.60.120.780, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR027228, PIAS2 IPR023321, PINIT IPR038654, PINIT_sf IPR003034, SAP_dom IPR036361, SAP_dom_sf IPR004181, Znf_MIZ IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR10782:SF12, PTHR10782:SF12, 1 hit |
Pfami | View protein in Pfam PF14324, PINIT, 1 hit PF02891, zf-MIZ, 1 hit |
SMARTi | View protein in SMART SM00513, SAP, 1 hit |
SUPFAMi | SSF68906, SSF68906, 1 hit |
PROSITEi | View protein in PROSITE PS51466, PINIT, 1 hit PS50800, SAP, 1 hit PS51044, ZF_SP_RING, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADFEELRNM VSSFRVSELQ VLLGFAGRNK SGRKHDLLMR ALHLLKSGCS
60 70 80 90 100
PAVQIKIREL YRRRYPRTLE GLCDLSTIKS SVFSLDGSSS PVEPDLPVAG
110 120 130 140 150
IHSLPSTSIT PHSPSSPVGS VLLQDTKPTF EMQQPSPPIP PVHPDVQLKN
160 170 180 190 200
LPFYDVLDVL IKPTSLVQSS IQRFQEKFFI FALTPQQVRE ICISRDFLPG
210 220 230 240 250
GRRDYTVQVQ LRLCLAETSC PQEDNYPNSL CIKVNGKLFP LPGYAPPPKN
260 270 280 290 300
GIEQKRPGRP LNITSLVRLS SAVPNQISIS WASEIGKNYS MSVYLVRQLT
310 320 330 340 350
SAMLLQRLKM KGIRNPDHSR ALIKEKLTAD PDSEIATTSL RVSLMCPLGK
360 370 380 390 400
MRLTIPCRAV TCTHLQCFDA ALYLQMNEKK PTWICPVCDK KAAYESLILD
410 420 430 440 450
GLFMEILNDC SDVDEIKFQE DGSWCPMRPK KEAMKVTSQP CTKVESSSVF
460 470 480 490 500
SKPCSVTVAS DASKKKIDVI DLTIESSSDE EEDPPAKRKC IFMSETQSSP
510 520 530 540 550
TKGVLMYQPS SVRVPSVTSV DPAAIPPSLT DYSVPFHHTP VSSMSSDLPG
560 570 580 590 600
LDFLSLIPVD PQYCPPMFLD SLTSPLTASS TSVTTTSPHE SSTHVSSSSS
610 620
RSETGVITSS GRNIPDIISL D
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8WHS8 | F8WHS8_MOUSE | E3 SUMO-protein ligase PIAS2 | Pias2 | 565 | Annotation score: | ||
G3UWE3 | G3UWE3_MOUSE | E3 SUMO-protein ligase PIAS2 | Pias2 | 614 | Annotation score: | ||
A0A494B929 | A0A494B929_MOUSE | E3 SUMO-protein ligase PIAS2 | Pias2 | 252 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 73 | C → S in AAF12825 (PubMed:11104669).Curated | 1 | ||
Sequence conflicti | 97 | P → A in AAF12825 (PubMed:11104669).Curated | 1 | ||
Sequence conflicti | 110 | T → A in AAF12825 (PubMed:11104669).Curated | 1 | ||
Sequence conflicti | 119 | G → E in AAF12825 (PubMed:11104669).Curated | 1 | ||
Sequence conflicti | 150 | N → T in AAF12825 (PubMed:11104669).Curated | 1 | ||
Sequence conflicti | 158 | D → E in AAF12825 (PubMed:11104669).Curated | 1 | ||
Sequence conflicti | 173 | R → G in BAC26579 (PubMed:16141072).Curated | 1 | ||
Sequence conflicti | 243 | G → A in AAF12825 (PubMed:11104669).Curated | 1 | ||
Sequence conflicti | 288 | N → Q in AAF12825 (PubMed:11104669).Curated | 1 | ||
Sequence conflicti | 320 | R → K in AAF12825 (PubMed:11104669).Curated | 1 | ||
Sequence conflicti | 327 | L → P in AAF12825 (PubMed:11104669).Curated | 1 | ||
Sequence conflicti | 375 | Q → R in AAF12825 (PubMed:11104669).Curated | 1 | ||
Sequence conflicti | 413 | V → A in AAF12825 (PubMed:11104669).Curated | 1 | ||
Sequence conflicti | 438 | S → I in BAC37407 (PubMed:16141072).Curated | 1 | ||
Sequence conflicti | 458 | V → G in AAF12825 (PubMed:11104669).Curated | 1 | ||
Sequence conflicti | 464 – 466 | KKK → GTR in AAF12825 (PubMed:11104669).Curated | 3 | ||
Isoform 2 (identifier: Q8C5D8-2) | |||||
Sequence conflicti | 555 | N → T in AAF12825 (PubMed:11104669).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012198 | 1 – 9 | Missing in isoform 3 and isoform 5. 1 Publication | 9 | |
Alternative sequenceiVSP_012199 | 551 – 572 | LDFLS…FLDSL → EQRRNDINNEVQLGASSDTV QQ in isoform 2 and isoform 3. 3 PublicationsAdd BLAST | 22 | |
Alternative sequenceiVSP_012200 | 563 – 580 | YCPPM…LTASS → SHLTLNSKQYVCHHHQPP in isoform 4. 1 PublicationAdd BLAST | 18 | |
Alternative sequenceiVSP_012201 | 573 – 621 | Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST | 49 | |
Alternative sequenceiVSP_012202 | 581 – 621 | Missing in isoform 4. 1 PublicationAdd BLAST | 41 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF201391 mRNA Translation: AAF12825.1 AK029716 mRNA Translation: BAC26579.1 AK014871 mRNA Translation: BAB29594.1 Frameshift. AK078813 mRNA Translation: BAC37407.1 AK086653 mRNA Translation: BAC39710.1 BC005596 mRNA Translation: AAH05596.1 BC034711 mRNA Translation: AAH34711.1 AF039567 mRNA Translation: AAB96678.1 Different initiation. |
CCDSi | CCDS37866.1 [Q8C5D8-1] |
RefSeqi | NP_001157639.1, NM_001164167.1 [Q8C5D8-4] NP_001157640.1, NM_001164168.1 NP_001157641.1, NM_001164169.1 [Q8C5D8-2] NP_001157642.1, NM_001164170.1 NP_032628.3, NM_008602.4 [Q8C5D8-1] |
Genome annotation databases
Ensembli | ENSMUST00000114777; ENSMUSP00000110425; ENSMUSG00000025423 [Q8C5D8-1] |
GeneIDi | 17344 |
KEGGi | mmu:17344 |
UCSCi | uc008frb.1, mouse [Q8C5D8-2] uc008frc.2, mouse [Q8C5D8-1] uc012bfb.1, mouse [Q8C5D8-4] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF201391 mRNA Translation: AAF12825.1 AK029716 mRNA Translation: BAC26579.1 AK014871 mRNA Translation: BAB29594.1 Frameshift. AK078813 mRNA Translation: BAC37407.1 AK086653 mRNA Translation: BAC39710.1 BC005596 mRNA Translation: AAH05596.1 BC034711 mRNA Translation: AAH34711.1 AF039567 mRNA Translation: AAB96678.1 Different initiation. |
CCDSi | CCDS37866.1 [Q8C5D8-1] |
RefSeqi | NP_001157639.1, NM_001164167.1 [Q8C5D8-4] NP_001157640.1, NM_001164168.1 NP_001157641.1, NM_001164169.1 [Q8C5D8-2] NP_001157642.1, NM_001164170.1 NP_032628.3, NM_008602.4 [Q8C5D8-1] |
3D structure databases
SMRi | Q8C5D8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201429, 14 interactors |
IntActi | Q8C5D8, 6 interactors |
MINTi | Q8C5D8 |
STRINGi | 10090.ENSMUSP00000110425 |
PTM databases
iPTMneti | Q8C5D8 |
PhosphoSitePlusi | Q8C5D8 |
Proteomic databases
EPDi | Q8C5D8 |
jPOSTi | Q8C5D8 |
PaxDbi | Q8C5D8 |
PRIDEi | Q8C5D8 |
Protocols and materials databases
Antibodypediai | 9110, 506 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000114777; ENSMUSP00000110425; ENSMUSG00000025423 [Q8C5D8-1] |
GeneIDi | 17344 |
KEGGi | mmu:17344 |
UCSCi | uc008frb.1, mouse [Q8C5D8-2] uc008frc.2, mouse [Q8C5D8-1] uc012bfb.1, mouse [Q8C5D8-4] |
Organism-specific databases
CTDi | 9063 |
MGIi | MGI:1096566, Pias2 |
Phylogenomic databases
eggNOGi | KOG2169, Eukaryota |
GeneTreei | ENSGT00990000203531 |
InParanoidi | Q8C5D8 |
OMAi | DPQQYCP |
OrthoDBi | 1205949at2759 |
PhylomeDBi | Q8C5D8 |
TreeFami | TF323787 |
Enzyme and pathway databases
UniPathwayi | UPA00886 |
Reactomei | R-MMU-3108214, SUMOylation of DNA damage response and repair proteins R-MMU-3232118, SUMOylation of transcription factors R-MMU-3232142, SUMOylation of ubiquitinylation proteins R-MMU-3899300, SUMOylation of transcription cofactors R-MMU-4090294, SUMOylation of intracellular receptors R-MMU-4551638, SUMOylation of chromatin organization proteins |
Miscellaneous databases
BioGRID-ORCSi | 17344, 3 hits in 17 CRISPR screens |
ChiTaRSi | Pias2, mouse |
PROi | PR:Q8C5D8 |
RNActi | Q8C5D8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025423, Expressed in testis and 314 other tissues |
ExpressionAtlasi | Q8C5D8, baseline and differential |
Genevisiblei | Q8C5D8, MM |
Family and domain databases
Gene3Di | 1.10.720.30, 1 hit 2.60.120.780, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR027228, PIAS2 IPR023321, PINIT IPR038654, PINIT_sf IPR003034, SAP_dom IPR036361, SAP_dom_sf IPR004181, Znf_MIZ IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR10782:SF12, PTHR10782:SF12, 1 hit |
Pfami | View protein in Pfam PF14324, PINIT, 1 hit PF02891, zf-MIZ, 1 hit |
SMARTi | View protein in SMART SM00513, SAP, 1 hit |
SUPFAMi | SSF68906, SSF68906, 1 hit |
PROSITEi | View protein in PROSITE PS51466, PINIT, 1 hit PS50800, SAP, 1 hit PS51044, ZF_SP_RING, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PIAS2_MOUSE | |
Accessioni | Q8C5D8Primary (citable) accession number: Q8C5D8 Secondary accession number(s): O54987 Q9QZ63 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 7, 2004 |
Last sequence update: | December 7, 2004 | |
Last modified: | December 2, 2020 | |
This is version 162 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families