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Protein

Signaling threshold-regulating transmembrane adapter 1

Gene

Sit1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Negatively regulates T-cell antigen receptor (TCR)-mediated signaling. Involved in positive selection of T-cells.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAdaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Signaling threshold-regulating transmembrane adapter 1
Alternative name(s):
SHP2-interacting transmembrane adapter protein
Suppression-inducing transmembrane adapter 1
Gene namesi
Name:Sit1
Synonyms:Sit
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1889342 Sit1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24ExtracellularSequence analysisAdd BLAST24
Transmembranei25 – 45Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini46 – 180CytoplasmicSequence analysisAdd BLAST135

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile, with normal growth and no obvious abnormalities. They show normal B-cell development and peripheral B-cell functions, but have mildly altered thymic development probably due to enhanced TCR signaling. This results in a greater susceptibility to autoimmune diseases.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000833411 – 180Signaling threshold-regulating transmembrane adapter 1Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi6N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi7InterchainBy similarity
Modified residuei63PhosphoserineBy similarity1
Modified residuei66PhosphoserineBy similarity1
Modified residuei73PhosphotyrosineBy similarity1
Modified residuei85PhosphoserineBy similarity1
Modified residuei90PhosphoserineBy similarity1
Modified residuei111PhosphotyrosineBy similarity1
Modified residuei132PhosphotyrosineBy similarity1
Modified residuei153PhosphotyrosineBy similarity1
Modified residuei166PhosphoserineCombined sources1
Modified residuei172PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosines upon TCR activation; which leads to the recruitment of PTPN11, GRB2 and CSK.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8C503
MaxQBiQ8C503
PaxDbiQ8C503
PeptideAtlasiQ8C503
PRIDEiQ8C503

PTM databases

iPTMnetiQ8C503
PhosphoSitePlusiQ8C503

Expressioni

Tissue specificityi

Expressed in thymus and spleen, with highest levels in immature thymocytes (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000028460 Expressed in 32 organ(s), highest expression level in thymus
CleanExiMM_SIT1
GenevisibleiQ8C503 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked. When phosphorylated, interacts with PTPN11/SHP2, GRB2 and CSK (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8C503, 4 interactors

Structurei

3D structure databases

SMRiQ8C503
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni73 – 76Interaction with GRB2By similarity4
Regioni130 – 135Interaction with PTPN11By similarity6
Regioni153 – 156Interaction with CSKBy similarity4
Regioni172 – 175Interaction with GRB2By similarity4

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYYR Eukaryota
ENOG4111C3Q LUCA
GeneTreeiENSGT00390000016476
HOGENOMiHOG000231524
HOVERGENiHBG080311
InParanoidiQ8C503
OMAiQAWGLWA
OrthoDBiEOG091G0U43
PhylomeDBiQ8C503
TreeFamiTF337816

Family and domain databases

InterProiView protein in InterPro
IPR033269 Sit1
PANTHERiPTHR15604 PTHR15604, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD358400 PD358400, 1 hit

Sequencei

Sequence statusi: Complete.

Q8C503-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRDYNCTTD DQLAWGIPSI SHAWGLWALL GVVTVLLLIS LAALLSQWTR
60 70 80 90 100
GRRRNQEGQG PLSGRSAEEV PLYGNLHYLQ TGRLSQEPRS EEQDPPSSGG
110 120 130 140 150
LARGAEEAMC YTSLQLRPAQ GRIPSSGNPI KYCEVVLDSE PKPQAPGPEP
160 170 180
ELYASVCAQT RRGRASFPDQ AYANSQPAPS
Length:180
Mass (Da):19,578
Last modified:March 1, 2003 - v1
Checksum:i7D1C0FD958B9BD03
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62L → H in CAB41506 (PubMed:10209036).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ236881 mRNA Translation: CAB41506.1
AK079882 mRNA Translation: BAC37773.1
BC064055 mRNA Translation: AAH64055.1
CCDSiCCDS18096.1
RefSeqiNP_062309.2, NM_019436.3
UniGeneiMm.31775

Genome annotation databases

EnsembliENSMUST00000030180; ENSMUSP00000030180; ENSMUSG00000028460
GeneIDi54390
KEGGimmu:54390
UCSCiuc008spw.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ236881 mRNA Translation: CAB41506.1
AK079882 mRNA Translation: BAC37773.1
BC064055 mRNA Translation: AAH64055.1
CCDSiCCDS18096.1
RefSeqiNP_062309.2, NM_019436.3
UniGeneiMm.31775

3D structure databases

SMRiQ8C503
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8C503, 4 interactors

PTM databases

iPTMnetiQ8C503
PhosphoSitePlusiQ8C503

Proteomic databases

EPDiQ8C503
MaxQBiQ8C503
PaxDbiQ8C503
PeptideAtlasiQ8C503
PRIDEiQ8C503

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030180; ENSMUSP00000030180; ENSMUSG00000028460
GeneIDi54390
KEGGimmu:54390
UCSCiuc008spw.2 mouse

Organism-specific databases

CTDi27240
MGIiMGI:1889342 Sit1

Phylogenomic databases

eggNOGiENOG410IYYR Eukaryota
ENOG4111C3Q LUCA
GeneTreeiENSGT00390000016476
HOGENOMiHOG000231524
HOVERGENiHBG080311
InParanoidiQ8C503
OMAiQAWGLWA
OrthoDBiEOG091G0U43
PhylomeDBiQ8C503
TreeFamiTF337816

Miscellaneous databases

PROiPR:Q8C503
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028460 Expressed in 32 organ(s), highest expression level in thymus
CleanExiMM_SIT1
GenevisibleiQ8C503 MM

Family and domain databases

InterProiView protein in InterPro
IPR033269 Sit1
PANTHERiPTHR15604 PTHR15604, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD358400 PD358400, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSIT1_MOUSE
AccessioniPrimary (citable) accession number: Q8C503
Secondary accession number(s): Q9WUU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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