UniProtKB - Q8C4Y3 (NELFB_MOUSE)
Negative elongation factor B
Nelfb
Functioni
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- cell population proliferation Source: UniProtKB
- negative regulation of stem cell differentiation Source: UniProtKB
- negative regulation of transcription elongation from RNA polymerase II promoter Source: UniProtKB
- stem cell differentiation Source: UniProtKB
Keywordsi
Molecular function | Repressor, RNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-112382, Formation of RNA Pol II elongation complex R-MMU-113418, Formation of the Early Elongation Complex R-MMU-674695, RNA Polymerase II Pre-transcription Events R-MMU-6796648, TP53 Regulates Transcription of DNA Repair Genes R-MMU-75955, RNA Polymerase II Transcription Elongation |
Names & Taxonomyi
Protein namesi | Recommended name: Negative elongation factor BShort name: NELF-B Alternative name(s): Cofactor of BRCA11 Publication |
Gene namesi | Name:Nelfb Synonyms:Cobra11 Publication ORF Names:MNCb-5210 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1931035, Nelfb |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytosol
- cytosol Source: Reactome
Mitochondrion
- mitochondrial outer membrane Source: Reactome
Nucleus
- NELF complex Source: UniProtKB
- nucleoplasm Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219130 | 1 – 580 | Negative elongation factor BAdd BLAST | 580 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 519 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 557 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8C4Y3 |
jPOSTi | Q8C4Y3 |
MaxQBi | Q8C4Y3 |
PaxDbi | Q8C4Y3 |
PeptideAtlasi | Q8C4Y3 |
PRIDEi | Q8C4Y3 |
ProteomicsDBi | 287369 [Q8C4Y3-1] 355156 |
PTM databases
iPTMneti | Q8C4Y3 |
PhosphoSitePlusi | Q8C4Y3 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000013465, Expressed in ear vesicle and 286 other tissues |
Genevisiblei | Q8C4Y3, MM |
Interactioni
Subunit structurei
The NELF complex is composed of NELFA, NELFB, NELFCD and NELFE; the N-terminus of NELFB binds to the NELFA:NELFCD subcomplex (By similarity). Binds RNA which may help to stabilize the NELF complex on nucleic acid (By similarity)
Interacts with the first BRCT repeat of BRCA1 (By similarity).
Interacts with KIAA1191 (By similarity). Isoform 1 and isoform 2 interact with NELFA, NELFCD and NELFE (PubMed:26010750).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 208386, 3 interactors |
STRINGi | 10090.ENSMUSP00000057731 |
Miscellaneous databases
RNActi | Q8C4Y3, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QTMJ, Eukaryota |
HOGENOMi | CLU_037919_0_0_1 |
InParanoidi | Q8C4Y3 |
OrthoDBi | 1095614at2759 |
PhylomeDBi | Q8C4Y3 |
TreeFami | TF324620 |
Family and domain databases
InterProi | View protein in InterPro IPR010405, COBRA1 |
PANTHERi | PTHR13503, PTHR13503, 1 hit |
Pfami | View protein in Pfam PF06209, COBRA1, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFAGLQDLGV ANGEDLKETL TNCTEPLKAI EQFQTENGVL LPSLQSALPF
60 70 80 90 100
LDLHGTPRLE FHQSVFDELR DKLLERVSAI ASEGKAEERY KKLEDLLEKS
110 120 130 140 150
FSLVKMPSLQ PVVMCVMKHL PKVPEKKLKL VMADKELYRA CAVEVKRQIW
160 170 180 190 200
QDNQALFGDE VSPLLKQYIL EKESALFSTE LSVLHNFFSP SPKTRRQGEV
210 220 230 240 250
VQKLTQMVGK NVKLYDMVLQ FLRTLFLRTR NVHYCTLRAE LLMSLHDLDV
260 270 280 290 300
SDICTVDPCH KFTWCLDACI RERFVDSKRA RELQGFLDGV KKGQEQVLGD
310 320 330 340 350
LSMILCDPFA INTLSLSTIR HLQELVSQET LPRDSPDLLL LLRLLALGQG
360 370 380 390 400
AWDLIDSQVF KEPKMEAELI TKFLPMLMSL VVDDFTFNVD QKLPAEEKAS
410 420 430 440 450
VTYPNTLPES FTKFLQEQRM ACEVGLYYVL HITKQRNKNA LLRLLPGLVE
460 470 480 490 500
TFGDLAFSDI FLHLLTGSLV LLADEFALED FCSSLFDGFF LTASPRKENV
510 520 530 540 550
HRHVLRLLLH LHARVAPSKL EALQKALEPT GQSGEAVKEL YSQLGEKLEQ
560 570 580
LDHRKPSPTQ AAETPALDLP LPSVPAPATL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6SWS9 | F6SWS9_MOUSE | Negative elongation factor B | Nelfb Cobra1 | 204 | Annotation score: | ||
F6UFF7 | F6UFF7_MOUSE | Negative elongation factor B | Nelfb Cobra1 | 144 | Annotation score: | ||
A0A5H1ZRL3 | A0A5H1ZRL3_MOUSE | Negative elongation factor B | Nelfb | 631 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 470 | V → A in BAA95090 (Ref. 4) Curated | 1 | |
Sequence conflicti | 547 | K → R in BAA95090 (Ref. 4) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_059999 | 1 | M → MATLEAAGERGLGGPRGTVE RASGAPSGSATAPAERGGDG VHSRASAGASAM in isoform 2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL732309 Genomic DNA No translation available. BC004762 mRNA Translation: AAH04762.1 Different initiation. AK080419 mRNA No translation available. AB041607 mRNA Translation: BAA95090.1 Different initiation. AK173124 mRNA Translation: BAD32402.1 |
RefSeqi | NP_001297086.1, NM_001310157.1 NP_067368.3, NM_021393.3 |
Genome annotation databases
GeneIDi | 58202 |
KEGGi | mmu:58202 |
UCSCi | uc008iqm.2, mouse [Q8C4Y3-1] |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL732309 Genomic DNA No translation available. BC004762 mRNA Translation: AAH04762.1 Different initiation. AK080419 mRNA No translation available. AB041607 mRNA Translation: BAA95090.1 Different initiation. AK173124 mRNA Translation: BAD32402.1 |
RefSeqi | NP_001297086.1, NM_001310157.1 NP_067368.3, NM_021393.3 |
3D structure databases
SMRi | Q8C4Y3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208386, 3 interactors |
STRINGi | 10090.ENSMUSP00000057731 |
PTM databases
iPTMneti | Q8C4Y3 |
PhosphoSitePlusi | Q8C4Y3 |
Proteomic databases
EPDi | Q8C4Y3 |
jPOSTi | Q8C4Y3 |
MaxQBi | Q8C4Y3 |
PaxDbi | Q8C4Y3 |
PeptideAtlasi | Q8C4Y3 |
PRIDEi | Q8C4Y3 |
ProteomicsDBi | 287369 [Q8C4Y3-1] 355156 |
Genome annotation databases
GeneIDi | 58202 |
KEGGi | mmu:58202 |
UCSCi | uc008iqm.2, mouse [Q8C4Y3-1] |
Organism-specific databases
CTDi | 25920 |
MGIi | MGI:1931035, Nelfb |
Phylogenomic databases
eggNOGi | ENOG502QTMJ, Eukaryota |
HOGENOMi | CLU_037919_0_0_1 |
InParanoidi | Q8C4Y3 |
OrthoDBi | 1095614at2759 |
PhylomeDBi | Q8C4Y3 |
TreeFami | TF324620 |
Enzyme and pathway databases
Reactomei | R-MMU-112382, Formation of RNA Pol II elongation complex R-MMU-113418, Formation of the Early Elongation Complex R-MMU-674695, RNA Polymerase II Pre-transcription Events R-MMU-6796648, TP53 Regulates Transcription of DNA Repair Genes R-MMU-75955, RNA Polymerase II Transcription Elongation |
Miscellaneous databases
BioGRID-ORCSi | 58202, 24 hits in 52 CRISPR screens |
ChiTaRSi | Nelfb, mouse |
PROi | PR:Q8C4Y3 |
RNActi | Q8C4Y3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000013465, Expressed in ear vesicle and 286 other tissues |
Genevisiblei | Q8C4Y3, MM |
Family and domain databases
InterProi | View protein in InterPro IPR010405, COBRA1 |
PANTHERi | PTHR13503, PTHR13503, 1 hit |
Pfami | View protein in Pfam PF06209, COBRA1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NELFB_MOUSE | |
Accessioni | Q8C4Y3Primary (citable) accession number: Q8C4Y3 Secondary accession number(s): A0A0X1KG62 Q9JJA5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 14, 2003 |
Last sequence update: | November 14, 2003 | |
Last modified: | April 7, 2021 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families