UniProtKB - Q8C432 (RN182_MOUSE)
Protein
E3 ubiquitin-protein ligase RNF182
Gene
Rnf182
Organism
Mus musculus (Mouse)
Status
Functioni
E3 ubiquitin-protein ligase that mediates the ubiquitination of ATP6V0C and targets it to degradation via the ubiquitin-proteasome pathway. Plays also a role in the inhibition of TLR-triggered innate immune response by mediating 'Lys'-48-linked ubiquitination and subsequent degradation of NF-kappa-B component RELA.By similarity
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 20 – 68 | RING-typePROSITE-ProRule annotationAdd BLAST | 49 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- protein ubiquitination Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase RNF182 (EC:2.3.2.27By similarity)Alternative name(s): RING finger protein 182 RING-type E3 ubiquitin transferase RNF182Curated |
Gene namesi | Name:Rnf182 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3045355, Rnf182 |
Subcellular locationi
Other locations
- Membrane By similarity; Multi-pass membrane protein By similarity
- Cytoplasm By similarity
Other locations
- cytoplasm Source: MGI
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 184 – 204 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 211 – 231 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000261621 | 1 – 247 | E3 ubiquitin-protein ligase RNF182Add BLAST | 247 |
Proteomic databases
PaxDbi | Q8C432 |
PRIDEi | Q8C432 |
PTM databases
PhosphoSitePlusi | Q8C432 |
Expressioni
Tissue specificityi
Expressed in the cortex, hippocampus, cerebellum and spinal cord, but not in the heart, liver, kidney or skeletal muscle.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000044164, Expressed in Ammon's horn and 175 other tissues |
Genevisiblei | Q8C432, MM |
Interactioni
Subunit structurei
Interacts with ATP6V0C.
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000062005 |
Miscellaneous databases
RNActi | Q8C432, protein |
Family & Domainsi
Domaini
The RING-type zinc finger domain is required for E3 ligase activity.By similarity
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 20 – 68 | RING-typePROSITE-ProRule annotationAdd BLAST | 49 |
Keywords - Domaini
Transmembrane, Transmembrane helix, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00730000111020 |
HOGENOMi | CLU_100624_0_0_1 |
InParanoidi | Q8C432 |
OMAi | FLDCMAL |
OrthoDBi | 1216224at2759 |
PhylomeDBi | Q8C432 |
TreeFami | TF331690 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR042285, RNF182 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR46675, PTHR46675, 1 hit |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8C432-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASQPLEEPA ESQASDELEC KICYNRYNLK QRKPKVLECC HRVCAKCLYK
60 70 80 90 100
IIDFGDSPQG VIVCPFCRFE TCLPDDEVSS LPDDNNILVN LTCGGKGKKC
110 120 130 140 150
LPENPTELLL TPKRLASLVS PSHTSSNCLV ITIMEVQRES SPSLSSTPVV
160 170 180 190 200
EFYRPASFDS VTTVSHNWTV WNCTSLLFQT SIRVLVWLLG LLYFSSLPLG
210 220 230 240
IYLLVSKKVT LGVVFVSLVP SSLVILMVYG FCQCVCHEFL DCMALPS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE0CZ87 | E0CZ87_MOUSE | E3 ubiquitin-protein ligase RNF182 | Rnf182 | 30 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK083175 mRNA Translation: BAC38795.1 BC089488 mRNA Translation: AAH89488.1 |
CCDSi | CCDS26480.1 |
RefSeqi | NP_899027.1, NM_183204.4 XP_006516996.1, XM_006516933.3 |
Genome annotation databases
Ensembli | ENSMUST00000059986; ENSMUSP00000062005; ENSMUSG00000044164 |
GeneIDi | 328234 |
KEGGi | mmu:328234 |
UCSCi | uc007qgh.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK083175 mRNA Translation: BAC38795.1 BC089488 mRNA Translation: AAH89488.1 |
CCDSi | CCDS26480.1 |
RefSeqi | NP_899027.1, NM_183204.4 XP_006516996.1, XM_006516933.3 |
3D structure databases
SMRi | Q8C432 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000062005 |
PTM databases
PhosphoSitePlusi | Q8C432 |
Proteomic databases
PaxDbi | Q8C432 |
PRIDEi | Q8C432 |
Protocols and materials databases
Antibodypediai | 1547, 128 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000059986; ENSMUSP00000062005; ENSMUSG00000044164 |
GeneIDi | 328234 |
KEGGi | mmu:328234 |
UCSCi | uc007qgh.2, mouse |
Organism-specific databases
CTDi | 221687 |
MGIi | MGI:3045355, Rnf182 |
Phylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00730000111020 |
HOGENOMi | CLU_100624_0_0_1 |
InParanoidi | Q8C432 |
OMAi | FLDCMAL |
OrthoDBi | 1216224at2759 |
PhylomeDBi | Q8C432 |
TreeFami | TF331690 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 328234, 0 hits in 17 CRISPR screens |
PROi | PR:Q8C432 |
RNActi | Q8C432, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000044164, Expressed in Ammon's horn and 175 other tissues |
Genevisiblei | Q8C432, MM |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR042285, RNF182 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR46675, PTHR46675, 1 hit |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RN182_MOUSE | |
Accessioni | Q8C432Primary (citable) accession number: Q8C432 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways