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Protein

Translation factor Guf1, mitochondrial

Gene

Guf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi57 – 64GTPUniRule annotation8
Nucleotide bindingi122 – 126GTPUniRule annotation5
Nucleotide bindingi176 – 179GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Translation factor Guf1, mitochondrialUniRule annotation (EC:3.6.5.-)
Alternative name(s):
Elongation factor 4 homologUniRule annotation
Short name:
EF-4UniRule annotation
GTPase Guf1UniRule annotation
Ribosomal back-translocaseUniRule annotation
Gene namesi
Name:Guf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2140726 Guf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 31MitochondrionUniRule annotationAdd BLAST31
ChainiPRO_000025625232 – 651Translation factor Guf1, mitochondrialAdd BLAST620

Proteomic databases

EPDiQ8C3X4
PaxDbiQ8C3X4
PeptideAtlasiQ8C3X4
PRIDEiQ8C3X4

PTM databases

iPTMnetiQ8C3X4
PhosphoSitePlusiQ8C3X4

Expressioni

Gene expression databases

BgeeiENSMUSG00000029208 Expressed in 222 organ(s), highest expression level in bone marrow
CleanExiMM_GUF1
ExpressionAtlasiQ8C3X4 baseline and differential
GenevisibleiQ8C3X4 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084480

Structurei

3D structure databases

ProteinModelPortaliQ8C3X4
SMRiQ8C3X4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 229tr-type GAdd BLAST182

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0462 Eukaryota
COG0481 LUCA
GeneTreeiENSGT00550000074940
HOGENOMiHOG000020624
HOVERGENiHBG081580
InParanoidiQ8C3X4
KOiK21594
OMAiALIFDSW
OrthoDBiEOG091G03BP
PhylomeDBiQ8C3X4
TreeFamiTF314751

Family and domain databases

CDDicd03709 lepA_C, 1 hit
Gene3Di3.30.70.2570, 1 hit
HAMAPiMF_00071 LepA, 1 hit
InterProiView protein in InterPro
IPR006297 EF-4
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR038363 LepA_C_sf
IPR013842 LepA_CTD
IPR035654 LepA_IV
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF06421 LepA_C, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR01393 lepA, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8C3X4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MWALVGRALA PWAAGARHAA ASEPRAACRL FSAAELKEKP DMSRFPVEDI
60 70 80 90 100
RNFSIIAHVD HGKSTLADRL LELTGTIDKT KKNKQVLDKL QVERERGITV
110 120 130 140 150
KAQTASLFYS FGGKQYLLNL IDTPGHVDFS YEVSRSLSAC QGVLLVVDAN
160 170 180 190 200
EGIQAQTVAN FFLAFEAQLS VIPVINKIDL KNADPERVGK QIEKVFDIPS
210 220 230 240 250
EECIKISAKL GTNVDSVLQA VIERIPPPKV HRENPLKALV FDSTFDQYRG
260 270 280 290 300
VIANIALFDG VVSKGDKIVS AHTKKAYEVN EVGILNPNEQ PTHKLYAGQV
310 320 330 340 350
GFLIAGMKDV TEAQIGDTLY LHNHPVEPLP GFKSAKPMVF AGVYPIDQSE
360 370 380 390 400
YNNLKSAIEK LTLNDSSVTV HRDSSLALGA GWRLGFLGLL HMEVFNQRLE
410 420 430 440 450
QEYNASVILT TPTVPYKAVL SSAKLIKEYK EKEITIINPA QFPEKSQVTE
460 470 480 490 500
YLEPVVLGTV ITPTEYTGKI MALCQARRAI QKNMTFIDEN RVMLKYLFPL
510 520 530 540 550
NEIVVDFYDS LKSLSSGYAS FDYEDAGYQT AELVKMDILL NGNMVEELVT
560 570 580 590 600
VVHREKAYTV GKSICERLKE SLPRQLYEIA IQAAVGSKVI ARETVKAYRK
610 620 630 640 650
NVLAKCYGGD ITRKMKLLKR QSEGKKKLRK IGNIEIPKDA FIKVLKTQPN

K
Length:651
Mass (Da):72,463
Last modified:March 1, 2003 - v1
Checksum:i7DA56A6C43753960
GO
Isoform 2 (identifier: Q8C3X4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     296-383: Missing.

Show »
Length:563
Mass (Da):62,933
Checksum:i75F2DB129305A977
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3UWY0G3UWY0_MOUSE
Translation factor Guf1, mitochondr...
Guf1
590Annotation score:
F6ZM03F6ZM03_MOUSE
Translation factor Guf1, mitochondr...
Guf1
296Annotation score:
A0A0J9YUM0A0A0J9YUM0_MOUSE
Translation factor Guf1, mitochondr...
Guf1
302Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021345296 – 383Missing in isoform 2. 1 PublicationAdd BLAST88

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053420 mRNA Translation: BAC35380.2
AK084627 mRNA Translation: BAC39234.1
BC057962 mRNA Translation: AAH57962.1
CCDSiCCDS19322.1 [Q8C3X4-1]
CCDS80296.1 [Q8C3X4-2]
RefSeqiNP_001297560.1, NM_001310631.1 [Q8C3X4-2]
NP_766299.1, NM_172711.3 [Q8C3X4-1]
UniGeneiMm.21105

Genome annotation databases

EnsembliENSMUST00000031113; ENSMUSP00000031113; ENSMUSG00000029208 [Q8C3X4-2]
ENSMUST00000087228; ENSMUSP00000084480; ENSMUSG00000029208 [Q8C3X4-1]
GeneIDi231279
KEGGimmu:231279
UCSCiuc008xqm.1 mouse [Q8C3X4-1]
uc008xqn.1 mouse [Q8C3X4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053420 mRNA Translation: BAC35380.2
AK084627 mRNA Translation: BAC39234.1
BC057962 mRNA Translation: AAH57962.1
CCDSiCCDS19322.1 [Q8C3X4-1]
CCDS80296.1 [Q8C3X4-2]
RefSeqiNP_001297560.1, NM_001310631.1 [Q8C3X4-2]
NP_766299.1, NM_172711.3 [Q8C3X4-1]
UniGeneiMm.21105

3D structure databases

ProteinModelPortaliQ8C3X4
SMRiQ8C3X4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084480

PTM databases

iPTMnetiQ8C3X4
PhosphoSitePlusiQ8C3X4

Proteomic databases

EPDiQ8C3X4
PaxDbiQ8C3X4
PeptideAtlasiQ8C3X4
PRIDEiQ8C3X4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031113; ENSMUSP00000031113; ENSMUSG00000029208 [Q8C3X4-2]
ENSMUST00000087228; ENSMUSP00000084480; ENSMUSG00000029208 [Q8C3X4-1]
GeneIDi231279
KEGGimmu:231279
UCSCiuc008xqm.1 mouse [Q8C3X4-1]
uc008xqn.1 mouse [Q8C3X4-2]

Organism-specific databases

CTDi60558
MGIiMGI:2140726 Guf1

Phylogenomic databases

eggNOGiKOG0462 Eukaryota
COG0481 LUCA
GeneTreeiENSGT00550000074940
HOGENOMiHOG000020624
HOVERGENiHBG081580
InParanoidiQ8C3X4
KOiK21594
OMAiALIFDSW
OrthoDBiEOG091G03BP
PhylomeDBiQ8C3X4
TreeFamiTF314751

Miscellaneous databases

ChiTaRSiGuf1 mouse
PROiPR:Q8C3X4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029208 Expressed in 222 organ(s), highest expression level in bone marrow
CleanExiMM_GUF1
ExpressionAtlasiQ8C3X4 baseline and differential
GenevisibleiQ8C3X4 MM

Family and domain databases

CDDicd03709 lepA_C, 1 hit
Gene3Di3.30.70.2570, 1 hit
HAMAPiMF_00071 LepA, 1 hit
InterProiView protein in InterPro
IPR006297 EF-4
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR038363 LepA_C_sf
IPR013842 LepA_CTD
IPR035654 LepA_IV
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF06421 LepA_C, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR01393 lepA, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGUF1_MOUSE
AccessioniPrimary (citable) accession number: Q8C3X4
Secondary accession number(s): Q6PEN7, Q8BPS6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: March 1, 2003
Last modified: October 10, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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