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Protein

F-box/LRR-repeat protein 5

Gene

Fbxl5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi15Iron 1By similarity1
Metal bindingi57Iron 1By similarity1
Metal bindingi58Iron 1By similarity1
Metal bindingi61Iron 1By similarity1
Metal bindingi61Iron 2By similarity1
Metal bindingi80Iron 2By similarity1
Metal bindingi126Iron 2By similarity1
Metal bindingi130Iron 1By similarity1
Metal bindingi130Iron 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway
LigandIron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-8951664 Neddylation
R-MMU-917937 Iron uptake and transport
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
F-box/LRR-repeat protein 5
Alternative name(s):
F-box and leucine-rich repeat protein 5
Gene namesi
Name:Fbxl5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2152883 Fbxl5

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198461 – 690F-box/LRR-repeat protein 5Add BLAST690

Post-translational modificationi

Ubiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor (By similarity).By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ8C2S5
PRIDEiQ8C2S5

PTM databases

PhosphoSitePlusiQ8C2S5

Expressioni

Gene expression databases

BgeeiENSMUSG00000039753 Expressed in 263 organ(s), highest expression level in intestine
CleanExiMM_FBXL5
ExpressionAtlasiQ8C2S5 baseline and differential
GenevisibleiQ8C2S5 MM

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with ACO1, IREB2/IRP2; the interaction depends on the 4Fe-4S cluster. Interacts with DCTN1/p150-glued (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045792

Structurei

3D structure databases

ProteinModelPortaliQ8C2S5
SMRiQ8C2S5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini202 – 248F-boxPROSITE-ProRule annotationAdd BLAST47
Repeati340 – 364LRR 1Add BLAST25
Repeati365 – 392LRR 2Add BLAST28
Repeati393 – 418LRR 3Add BLAST26
Repeati478 – 507LRR 4Add BLAST30
Repeati575 – 606LRR 5Add BLAST32
Repeati607 – 634LRR 6Add BLAST28
Repeati635 – 660LRR 7Add BLAST26

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 159Hemerythrin-likeBy similarityAdd BLAST159

Domaini

The hemerythrin-like region acts as an oxygen and iron sensor by binding oxygen through a diiron metal-center. In absence of oxygen and iron, the protein is ubiquitinated and degraded (By similarity).By similarity

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiENOG410IHDI Eukaryota
ENOG410ZKEM LUCA
GeneTreeiENSGT00390000006172
HOVERGENiHBG051589
InParanoidiQ8C2S5
KOiK10271
OMAiLNDEHFY
OrthoDBiEOG091G02J5
PhylomeDBiQ8C2S5
TreeFamiTF331105

Family and domain databases

Gene3Di3.80.10.10, 2 hits
InterProiView protein in InterPro
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR012312 Haemerythrin-like
IPR001611 Leu-rich_rpt
IPR006553 Leu-rich_rpt_Cys-con_subtyp
IPR032675 LRR_dom_sf
PfamiView protein in Pfam
PF12937 F-box-like, 1 hit
PF01814 Hemerythrin, 1 hit
PF13516 LRR_6, 2 hits
SMARTiView protein in SMART
SM00256 FBOX, 1 hit
SM00367 LRR_CC, 4 hits
SUPFAMiSSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS50181 FBOX, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8C2S5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAPFPDEVDV FTAPHWRMKQ LVGRYCDKLS KTNFSNNNDF RALLQSLYAT
60 70 80 90 100
FKEFKMHEQI ENEYIIGLLQ QRSQTIYNVH SDNKLSEMLS LFEKGLKNVK
110 120 130 140 150
NEYEQLNYAK QLKERLEAFT RDFLPHMKEE EEVFQPMLME YFTYEELKDI
160 170 180 190 200
KKKVIAQHCS QKDTAELLRG LSLWNQAEER QKVLKYSVDE KADTEAEVSE
210 220 230 240 250
HSTGITHLPP EVMLSIFSYL NPQELCRCSQ VSTKWSQLAK TGSLWKHLYP
260 270 280 290 300
VHWARGDWYS GPATELDTEP DEEWVRNRKD ESRAFQEWDE DADIDESEES
310 320 330 340 350
AEESVAISIA QMEKRVLHGL IHNVLPYVGT SVKTLVLAYS SAVSSKMVRQ
360 370 380 390 400
ILELCPNLEH LDLTQTDISD SAFDSWSWLG CCQSLRHLDL SGCEKITDMA
410 420 430 440 450
LEKISRALGV LTSHQSGFLK SAGKAASTPW TSKDITMPST TQYACLHNLT
460 470 480 490 500
DKGIGEEIDN EHSWTEPVSS ESLTSPYVWM LDAEDLADIE DAVEWRHRNV
510 520 530 540 550
ESLCVMETAS NFGCSSSGCY SKDIVGLRTS VCWQQHCASP AFAYCGHSFC
560 570 580 590 600
CTGTALRTMT TLPATSAMCR KALRTTLPRG KDLIYFGSEK SDQETGRVLL
610 620 630 640 650
FLSLSGCYQI TDHGLRALTL GGGLPYLEHL NLSGCLTVTG AGLQDLVSAC
660 670 680 690
PSLNDEYFYY CDNINGPHAD TASGCQNLQC GFRACCRSGE
Length:690
Mass (Da):77,894
Last modified:October 3, 2003 - v2
Checksum:i9558476AFB3ECCA0
GO
Isoform 2 (identifier: Q8C2S5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: MAPFPDEVDVFTAPHWRMKQLVGRYCDK → MRNNQELKYEIKWKHLDCSVVE

Note: No experimental confirmation available.
Show »
Length:684
Mass (Da):77,319
Checksum:iB1B47D533E3FC8DA
GO
Isoform 3 (identifier: Q8C2S5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     617-623: ALTLGGG → WVISPSC
     624-690: Missing.

Show »
Length:623
Mass (Da):70,924
Checksum:i9DAE11360271A8AE
GO
Isoform 4 (identifier: Q8C2S5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     298-298: Missing.

Note: No experimental confirmation available.
Show »
Length:689
Mass (Da):77,765
Checksum:i47DD6E9530CD54F7
GO
Isoform 5 (identifier: Q8C2S5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     29-71: Missing.
     617-623: ALTLGGG → WVISPSC
     624-690: Missing.

Note: No experimental confirmation available.
Show »
Length:580
Mass (Da):65,804
Checksum:iB024C4DE375A3E7C
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z584D3Z584_MOUSE
F-box/LRR-repeat protein 5
Fbxl5
673Annotation score:
F7BZC4F7BZC4_MOUSE
F-box/LRR-repeat protein 5
Fbxl5
679Annotation score:
F6W6I1F6W6I1_MOUSE
F-box/LRR-repeat protein 5
Fbxl5
611Annotation score:
A0A0G2JE06A0A0G2JE06_MOUSE
F-box/LRR-repeat protein 5
Fbxl5
107Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105Q → R in BAC38698 (PubMed:16141072).Curated1
Sequence conflicti127M → K in BAC40126 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0084181 – 28MAPFP…RYCDK → MRNNQELKYEIKWKHLDCSV VE in isoform 2. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_03852929 – 71Missing in isoform 5. 1 PublicationAdd BLAST43
Alternative sequenceiVSP_038530298Missing in isoform 4. 1 Publication1
Alternative sequenceiVSP_008419617 – 623ALTLGGG → WVISPSC in isoform 3 and isoform 5. 2 Publications7
Alternative sequenceiVSP_008420624 – 690Missing in isoform 3 and isoform 5. 2 PublicationsAdd BLAST67

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029957 mRNA Translation: BAC26698.1
AK041497 mRNA Translation: BAC30964.1
AK082931 mRNA Translation: BAC38698.1
AK085100 mRNA Translation: BAC39366.1
AK088067 mRNA Translation: BAC40126.1
AK150664 mRNA Translation: BAE29748.1
AK159744 mRNA Translation: BAE35337.1
AK171783 mRNA Translation: BAE42662.1
BC047214 mRNA Translation: AAH47214.1
BC113798 mRNA Translation: AAI13799.1
CCDSiCCDS19263.1 [Q8C2S5-3]
CCDS51489.1 [Q8C2S5-1]
RefSeqiNP_001153435.1, NM_001159963.1 [Q8C2S5-1]
NP_848844.1, NM_178729.4 [Q8C2S5-3]
UniGeneiMm.25794

Genome annotation databases

EnsembliENSMUST00000047857; ENSMUSP00000045792; ENSMUSG00000039753 [Q8C2S5-1]
ENSMUST00000087465; ENSMUSP00000084733; ENSMUSG00000039753 [Q8C2S5-3]
ENSMUST00000114047; ENSMUSP00000109681; ENSMUSG00000039753 [Q8C2S5-2]
ENSMUST00000121736; ENSMUSP00000112444; ENSMUSG00000039753 [Q8C2S5-5]
ENSMUST00000196483; ENSMUSP00000143703; ENSMUSG00000039753 [Q8C2S5-4]
GeneIDi242960
KEGGimmu:242960
UCSCiuc008xhu.2 mouse [Q8C2S5-1]
uc008xhv.2 mouse [Q8C2S5-4]
uc008xhw.2 mouse [Q8C2S5-3]
uc008xhx.2 mouse [Q8C2S5-5]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029957 mRNA Translation: BAC26698.1
AK041497 mRNA Translation: BAC30964.1
AK082931 mRNA Translation: BAC38698.1
AK085100 mRNA Translation: BAC39366.1
AK088067 mRNA Translation: BAC40126.1
AK150664 mRNA Translation: BAE29748.1
AK159744 mRNA Translation: BAE35337.1
AK171783 mRNA Translation: BAE42662.1
BC047214 mRNA Translation: AAH47214.1
BC113798 mRNA Translation: AAI13799.1
CCDSiCCDS19263.1 [Q8C2S5-3]
CCDS51489.1 [Q8C2S5-1]
RefSeqiNP_001153435.1, NM_001159963.1 [Q8C2S5-1]
NP_848844.1, NM_178729.4 [Q8C2S5-3]
UniGeneiMm.25794

3D structure databases

ProteinModelPortaliQ8C2S5
SMRiQ8C2S5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045792

PTM databases

PhosphoSitePlusiQ8C2S5

Proteomic databases

PaxDbiQ8C2S5
PRIDEiQ8C2S5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047857; ENSMUSP00000045792; ENSMUSG00000039753 [Q8C2S5-1]
ENSMUST00000087465; ENSMUSP00000084733; ENSMUSG00000039753 [Q8C2S5-3]
ENSMUST00000114047; ENSMUSP00000109681; ENSMUSG00000039753 [Q8C2S5-2]
ENSMUST00000121736; ENSMUSP00000112444; ENSMUSG00000039753 [Q8C2S5-5]
ENSMUST00000196483; ENSMUSP00000143703; ENSMUSG00000039753 [Q8C2S5-4]
GeneIDi242960
KEGGimmu:242960
UCSCiuc008xhu.2 mouse [Q8C2S5-1]
uc008xhv.2 mouse [Q8C2S5-4]
uc008xhw.2 mouse [Q8C2S5-3]
uc008xhx.2 mouse [Q8C2S5-5]

Organism-specific databases

CTDi26234
MGIiMGI:2152883 Fbxl5

Phylogenomic databases

eggNOGiENOG410IHDI Eukaryota
ENOG410ZKEM LUCA
GeneTreeiENSGT00390000006172
HOVERGENiHBG051589
InParanoidiQ8C2S5
KOiK10271
OMAiLNDEHFY
OrthoDBiEOG091G02J5
PhylomeDBiQ8C2S5
TreeFamiTF331105

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-8951664 Neddylation
R-MMU-917937 Iron uptake and transport
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiFbxl5 mouse
PROiPR:Q8C2S5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039753 Expressed in 263 organ(s), highest expression level in intestine
CleanExiMM_FBXL5
ExpressionAtlasiQ8C2S5 baseline and differential
GenevisibleiQ8C2S5 MM

Family and domain databases

Gene3Di3.80.10.10, 2 hits
InterProiView protein in InterPro
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR012312 Haemerythrin-like
IPR001611 Leu-rich_rpt
IPR006553 Leu-rich_rpt_Cys-con_subtyp
IPR032675 LRR_dom_sf
PfamiView protein in Pfam
PF12937 F-box-like, 1 hit
PF01814 Hemerythrin, 1 hit
PF13516 LRR_6, 2 hits
SMARTiView protein in SMART
SM00256 FBOX, 1 hit
SM00367 LRR_CC, 4 hits
SUPFAMiSSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS50181 FBOX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFBXL5_MOUSE
AccessioniPrimary (citable) accession number: Q8C2S5
Secondary accession number(s): Q14CG1
, Q3TAK6, Q3TWC9, Q3UC66, Q80XI5, Q8BGF5, Q8BNL3, Q8C3Q8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: November 7, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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