Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tubulinyl-Tyr carboxypeptidase 1

Gene

Vash1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tyrosine carboxypeptidase that removes the C-terminal tyrosine residue of alpha-tubulin, thereby regulating microtubule dynamics and function (PubMed:29146868). Acts as an angiogenesis inhibitor: inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis (PubMed:19204325). This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts (By similarity).By similarity2 Publications

Catalytic activityi

Cleavage of the -Glu-|-Tyr bond to release the C-terminal tyrosine residue from the native tyrosinated tubulin. Inactive on Z-Glu-Tyr.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1791 Publication1 Publication1
Active sitei2141 Publication1
Active sitei2311 Publication1

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • metallocarboxypeptidase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease
Biological processCell cycle, Growth arrest

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulinyl-Tyr carboxypeptidase 1Curated (EC:3.4.17.171 Publication)
Alternative name(s):
Tyrosine carboxypeptidase 1Curated
Short name:
TTCP 1Curated
Vasohibin-11 Publication
Gene namesi
Name:Vash1Imported
Synonyms:Vash
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2442543 Vash1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi179C → A: Abolished tyrosine carboxypeptidase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001899811 – 375Tubulinyl-Tyr carboxypeptidase 1Add BLAST375

Post-translational modificationi

Ubiquitinated in vitro.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei39 – 40CleavageBy similarity2
Sitei86 – 87CleavageBy similarity2

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ8C1W1
PaxDbiQ8C1W1
PRIDEiQ8C1W1

PTM databases

iPTMnetiQ8C1W1
PhosphoSitePlusiQ8C1W1

Expressioni

Tissue specificityi

Expressed at low level in proliferating endothelial cells at the sprouting front but highly expressed in nonproliferating endothelial cells in the termination zone.1 Publication

Gene expression databases

BgeeiENSMUSG00000021256 Expressed in 29 organ(s), highest expression level in cerebral cortex marginal layer
CleanExiMM_VASH1

Interactioni

Subunit structurei

Interacts with SVBP; interaction enhances VASH1 tyrosine carboxypeptidase activity.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021681

Structurei

3D structure databases

ProteinModelPortaliQ8C1W1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni329 – 375Involved in heparin-binding and antiangiogenic activityBy similarityAdd BLAST47

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFRP Eukaryota
ENOG410ZG80 LUCA
GeneTreeiENSGT00390000012703
HOGENOMiHOG000007477
HOVERGENiHBG079337
InParanoidiQ8C1W1
OMAiIEWKHSI
OrthoDBiEOG091G0CSJ
TreeFamiTF329370

Family and domain databases

InterProiView protein in InterPro
IPR028131 Vasohibin
IPR028132 Vasohibin-1
PANTHERiPTHR15750 PTHR15750, 1 hit
PTHR15750:SF2 PTHR15750:SF2, 1 hit
PfamiView protein in Pfam
PF14822 Vasohibin, 1 hit

Sequencei

Sequence statusi: Complete.

Q8C1W1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPGGKKVVPS GSSSASPNAA ATTTAAAAAA AAAPHSGTKR LETTEGASAQ
60 70 80 90 100
RDEEPEEEGE EDLRDGGVPF FINRGGLPVD EATWERMWKH VAKIHPDGEK
110 120 130 140 150
VALRIRGATD LPKIPIPSVP TFQPTTPVPE RLEAVQRYIR ELQYNHTGTQ
160 170 180 190 200
FFEIKKSRPL TGLMDLAKEM TKEALPIKCL EAVILGIYLT NSMPTLERFP
210 220 230 240 250
ISFKTYFSGN YFRHIVLGVN FGGRYGALGM SRREDLMYKP PAFRTLSELV
260 270 280 290 300
LDYEAAYGRC WHVLKKVKLG QCVSHDPHSV EQIEWKHSVL DVERLGREDF
310 320 330 340 350
RKELERHARD MRLKIGKGTG PPSPTKDRKK DVSSPQRAQS SPHRRNSRSE
360 370
RRPSGEKKPA EPKAMPDLSG YQIRV
Length:375
Mass (Da):41,875
Last modified:July 27, 2011 - v4
Checksum:i82F7348A17B32B6D
GO

Sequence cautioni

The sequence BAC41121 differs from that shown. Reason: Frameshift at position 27.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2P → A in BAC41121 (PubMed:16141072).Curated1
Sequence conflicti157S → G in BAC41121 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090166 mRNA Translation: BAC41121.1 Frameshift.
AC102689 Genomic DNA No translation available.
CCDSiCCDS49118.1
RefSeqiNP_796328.2, NM_177354.4
UniGeneiMm.216101

Genome annotation databases

EnsembliENSMUST00000021681; ENSMUSP00000021681; ENSMUSG00000021256
GeneIDi238328
KEGGimmu:238328
UCSCiuc007ohx.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090166 mRNA Translation: BAC41121.1 Frameshift.
AC102689 Genomic DNA No translation available.
CCDSiCCDS49118.1
RefSeqiNP_796328.2, NM_177354.4
UniGeneiMm.216101

3D structure databases

ProteinModelPortaliQ8C1W1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021681

PTM databases

iPTMnetiQ8C1W1
PhosphoSitePlusiQ8C1W1

Proteomic databases

MaxQBiQ8C1W1
PaxDbiQ8C1W1
PRIDEiQ8C1W1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021681; ENSMUSP00000021681; ENSMUSG00000021256
GeneIDi238328
KEGGimmu:238328
UCSCiuc007ohx.2 mouse

Organism-specific databases

CTDi22846
MGIiMGI:2442543 Vash1

Phylogenomic databases

eggNOGiENOG410IFRP Eukaryota
ENOG410ZG80 LUCA
GeneTreeiENSGT00390000012703
HOGENOMiHOG000007477
HOVERGENiHBG079337
InParanoidiQ8C1W1
OMAiIEWKHSI
OrthoDBiEOG091G0CSJ
TreeFamiTF329370

Miscellaneous databases

ChiTaRSiVash1 mouse
PROiPR:Q8C1W1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021256 Expressed in 29 organ(s), highest expression level in cerebral cortex marginal layer
CleanExiMM_VASH1

Family and domain databases

InterProiView protein in InterPro
IPR028131 Vasohibin
IPR028132 Vasohibin-1
PANTHERiPTHR15750 PTHR15750, 1 hit
PTHR15750:SF2 PTHR15750:SF2, 1 hit
PfamiView protein in Pfam
PF14822 Vasohibin, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVASH1_MOUSE
AccessioniPrimary (citable) accession number: Q8C1W1
Secondary accession number(s): E9QKT9, Q8C394
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 99 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again