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Protein

Protein IWS1 homolog

Gene

Iws1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing, mRNA transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-75955 RNA Polymerase II Transcription Elongation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein IWS1 homolog
Alternative name(s):
IWS1-like protein
Gene namesi
Name:Iws1
Synonyms:Iws1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1920723 Iws1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000833471 – 766Protein IWS1 homologAdd BLAST766

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei27PhosphoserineBy similarity1
Modified residuei54PhosphoserineBy similarity1
Modified residuei69PhosphoserineBy similarity1
Modified residuei157PhosphoserineBy similarity1
Modified residuei170PhosphoserineBy similarity1
Modified residuei172PhosphoserineBy similarity1
Modified residuei183PhosphoserineBy similarity1
Modified residuei196PhosphoserineCombined sources1
Modified residuei198PhosphoserineBy similarity1
Modified residuei209PhosphoserineCombined sources1
Modified residuei211PhosphoserineCombined sources1
Modified residuei222PhosphoserineCombined sources1
Modified residuei224PhosphoserineBy similarity1
Modified residuei235PhosphoserineCombined sources1
Modified residuei237PhosphoserineCombined sources1
Modified residuei248PhosphoserineBy similarity1
Modified residuei250PhosphoserineBy similarity1
Modified residuei252PhosphoserineBy similarity1
Modified residuei261PhosphoserineCombined sources1
Modified residuei263PhosphoserineCombined sources1
Modified residuei277PhosphoserineBy similarity1
Modified residuei280PhosphoserineBy similarity1
Modified residuei295PhosphoserineBy similarity1
Modified residuei306PhosphoserineCombined sources1
Modified residuei307PhosphoserineCombined sources1
Modified residuei309PhosphoserineCombined sources1
Modified residuei321PhosphoserineCombined sources1
Modified residuei343PhosphoserineCombined sources1
Modified residuei345PhosphoserineCombined sources1
Modified residuei366PhosphoserineCombined sources1
Modified residuei368PhosphoserineCombined sources1
Modified residuei372PhosphoserineCombined sources1
Modified residuei381PhosphothreonineBy similarity1
Modified residuei384PhosphoserineCombined sources1
Modified residuei386PhosphoserineCombined sources1
Modified residuei427PhosphoserineBy similarity1
Modified residuei458PhosphoserineBy similarity1
Modified residuei460PhosphoserineBy similarity1
Modified residuei672PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8C1D8
PaxDbiQ8C1D8
PeptideAtlasiQ8C1D8
PRIDEiQ8C1D8

PTM databases

iPTMnetiQ8C1D8
PhosphoSitePlusiQ8C1D8

Expressioni

Tissue specificityi

Ubiquitous. Expressed at highest level in kidney, then testicle, large intestine, small intestine, spleen and prostate, whereas the lowest level is detected in heart.1 Publication

Gene expression databases

BgeeiENSMUSG00000024384 Expressed in 245 organ(s), highest expression level in blastocyst
CleanExiMM_IWS1
ExpressionAtlasiQ8C1D8 baseline and differential
GenevisibleiQ8C1D8 MM

Interactioni

Subunit structurei

Interacts with SUPT6H; binds preferentially to the POLR2A-bound SUPT6H. Interacts with ALYREF/THOC4, SETD2 and PRMT5 (By similarity). Interacts with HDGFRP2 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8C1D8, 3 interactors
MINTiQ8C1D8
STRINGi10090.ENSMUSP00000025243

Structurei

3D structure databases

ProteinModelPortaliQ8C1D8
SMRiQ8C1D8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini561 – 639TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni470 – 766Interaction with SUPT6H and ALYREFBy similarityAdd BLAST297

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi83 – 456Glu-richAdd BLAST374

Sequence similaritiesi

Belongs to the IWS1 family.Curated

Phylogenomic databases

eggNOGiKOG1793 Eukaryota
COG5139 LUCA
GeneTreeiENSGT00720000108834
HOGENOMiHOG000113120
HOVERGENiHBG081811
InParanoidiQ8C1D8
KOiK17498
OMAiNEDVNQH
OrthoDBiEOG091G0K1Z
PhylomeDBiQ8C1D8
TreeFamiTF315504

Family and domain databases

Gene3Di1.20.930.10, 1 hit
InterProiView protein in InterPro
IPR035441 TFIIS/LEDGF_dom_sf
IPR017923 TFIIS_N
PfamiView protein in Pfam
PF08711 Med26, 1 hit
SUPFAMiSSF47676 SSF47676, 1 hit
PROSITEiView protein in PROSITE
PS51319 TFIIS_N, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8C1D8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDSEYYSGDQ SDDGGATPVQ DERDSGSDGE DGVTEQHSGS DTGSVDHHSE
60 70 80 90 100
NETSDREDGL AKIHNGTDSE NDEPSNANAS DSESEELHRP KDSDSDSEEH
110 120 130 140 150
AESPASDSEN EPVNQHGSDS ENEELLNGHA SDSEKEEVSK HAASDSEAED
160 170 180 190 200
TLQPQVSESD SEDPPRPQAS DSENEEPPKP RISDSESEEL PKPRVSDSES
210 220 230 240 250
EDPPRPQASD SESEELPKPR VSDSESEDPP RPQASDSESE ELPKPRVSDS
260 270 280 290 300
ESEDPQKGPA SDSEAEDASR HKEKPDSDDS DGENKREDSE VQNESDGHTD
310 320 330 340 350
RKGLHSSDSE EEEPKRQKID SDDDEEKEGD EEKVAKRKAA VLSDSEDDAG
360 370 380 390 400
NASAKKSRVV CDADDSDSDV VSDKSGKRET TVASDSEEEA GKEESSVKKK
410 420 430 440 450
EDKDLFGSDS ESGNEEENLI ADIFGESGDE EEEEFTGFNQ EDLEEEKNET
460 470 480 490 500
QLKEAEDSDS DDNIKRGKHM DFLSDFEMML QRKKSMCGKR RRNRDGGTFI
510 520 530 540 550
SDADDVVSAM IVKMNEAAEE DRQLNNQKKP ALKKLTLLPT VVMHLKKQDL
560 570 580 590 600
KETFIDSGVM SAIKEWLSPL PDRSLPALKI REELLKILQE LPSVSQETLK
610 620 630 640 650
HSGIGRAVMY LYKHPKESRS NKDMAGKLIN EWSRPIFGLT SNYKGMTREE
660 670 680 690 700
REQRDLEQMP QRRRLSSTGG QTPRRDLEKV LTGEEKALRP GDPGFCARAR
710 720 730 740 750
VPMPSNKDYV VRPKWNVEME SSRFQASSKK GISRLDKQMR KFTDIRKKSR
760
SAHAVKISIE GNKMPL
Length:766
Mass (Da):85,248
Last modified:March 1, 2003 - v1
Checksum:i5787745679C3A3A7
GO
Isoform 2 (identifier: Q8C1D8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     520-543: EDRQLNNQKKPALKKLTLLPTVVM → VSSSRLPFSPPALNQIDGVANLSL
     544-766: Missing.

Note: No experimental confirmation available.
Show »
Length:543
Mass (Da):59,298
Checksum:i21025D95E6580C2F
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1D5RLV0A0A1D5RLV0_MOUSE
Protein IWS1 homolog
Iws1
765Annotation score:
A0A1D5RMB4A0A1D5RMB4_MOUSE
Protein IWS1 homolog
Iws1
198Annotation score:
A0A1D5RM16A0A1D5RM16_MOUSE
Protein IWS1 homolog
Iws1
155Annotation score:
A0A1D5RLS6A0A1D5RLS6_MOUSE
Protein IWS1 homolog
Iws1
91Annotation score:

Sequence cautioni

The sequence BAB24793 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE23006 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti260 – 261AS → VI in BAC31034 (PubMed:16141072).Curated2
Sequence conflicti289S → F in BAC31034 (PubMed:16141072).Curated1
Sequence conflicti480L → T in BAB24793 (PubMed:16141072).Curated1
Sequence conflicti578L → M in BAB24793 (PubMed:16141072).Curated1
Sequence conflicti700R → S in BAE23006 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016994520 – 543EDRQL…PTVVM → VSSSRLPFSPPALNQIDGVA NLSL in isoform 2. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_016995544 – 766Missing in isoform 2. 1 PublicationAdd BLAST223

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006925 mRNA Translation: BAB24793.1 Different initiation.
AK028247 mRNA Translation: BAC25838.1
AK041691 mRNA Translation: BAC31034.1
AK136492 mRNA Translation: BAE23006.1 Different initiation.
AK163972 mRNA Translation: BAE37560.1
BC068184 mRNA Translation: AAH68184.1
CCDSiCCDS29115.1 [Q8C1D8-1]
RefSeqiNP_775617.1, NM_173441.3 [Q8C1D8-1]
UniGeneiMm.66853

Genome annotation databases

EnsembliENSMUST00000025243; ENSMUSP00000025243; ENSMUSG00000024384 [Q8C1D8-1]
GeneIDi73473
KEGGimmu:73473
UCSCiuc008eiy.2 mouse [Q8C1D8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006925 mRNA Translation: BAB24793.1 Different initiation.
AK028247 mRNA Translation: BAC25838.1
AK041691 mRNA Translation: BAC31034.1
AK136492 mRNA Translation: BAE23006.1 Different initiation.
AK163972 mRNA Translation: BAE37560.1
BC068184 mRNA Translation: AAH68184.1
CCDSiCCDS29115.1 [Q8C1D8-1]
RefSeqiNP_775617.1, NM_173441.3 [Q8C1D8-1]
UniGeneiMm.66853

3D structure databases

ProteinModelPortaliQ8C1D8
SMRiQ8C1D8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8C1D8, 3 interactors
MINTiQ8C1D8
STRINGi10090.ENSMUSP00000025243

PTM databases

iPTMnetiQ8C1D8
PhosphoSitePlusiQ8C1D8

Proteomic databases

EPDiQ8C1D8
PaxDbiQ8C1D8
PeptideAtlasiQ8C1D8
PRIDEiQ8C1D8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025243; ENSMUSP00000025243; ENSMUSG00000024384 [Q8C1D8-1]
GeneIDi73473
KEGGimmu:73473
UCSCiuc008eiy.2 mouse [Q8C1D8-1]

Organism-specific databases

CTDi55677
MGIiMGI:1920723 Iws1

Phylogenomic databases

eggNOGiKOG1793 Eukaryota
COG5139 LUCA
GeneTreeiENSGT00720000108834
HOGENOMiHOG000113120
HOVERGENiHBG081811
InParanoidiQ8C1D8
KOiK17498
OMAiNEDVNQH
OrthoDBiEOG091G0K1Z
PhylomeDBiQ8C1D8
TreeFamiTF315504

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-75955 RNA Polymerase II Transcription Elongation

Miscellaneous databases

PROiPR:Q8C1D8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024384 Expressed in 245 organ(s), highest expression level in blastocyst
CleanExiMM_IWS1
ExpressionAtlasiQ8C1D8 baseline and differential
GenevisibleiQ8C1D8 MM

Family and domain databases

Gene3Di1.20.930.10, 1 hit
InterProiView protein in InterPro
IPR035441 TFIIS/LEDGF_dom_sf
IPR017923 TFIIS_N
PfamiView protein in Pfam
PF08711 Med26, 1 hit
SUPFAMiSSF47676 SSF47676, 1 hit
PROSITEiView protein in PROSITE
PS51319 TFIIS_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIWS1_MOUSE
AccessioniPrimary (citable) accession number: Q8C1D8
Secondary accession number(s): Q3TQ25
, Q3UWB0, Q6NVD1, Q8BY73, Q9D9H4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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