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Protein

N-fatty-acyl-amino acid synthase/hydrolase PM20D1

Gene

Pm20d1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids. These metabolites are endogenous chemical uncouplers of mitochondrial respiration. In an UCP1-independent manner, maybe through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. Thereby, this secreted protein may indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.1 Publication

Miscellaneous

Overexpression in mice blunts, high fat diet-induced, fat mass increase and associated weight gain.1 Publication

Catalytic activityi

A N-fatty-acyl-L-amino acid + H2O = a fatty acid + an amino acid.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi125Zinc 2By similarity1
Active sitei127By similarity1
Metal bindingi157Zinc 1By similarity1
Metal bindingi157Zinc 2By similarity1
Active sitei191Proton acceptorBy similarity1
Metal bindingi192Zinc 1By similarity1
Metal bindingi218Zinc 2By similarity1
Metal bindingi465Zinc 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM20.011

Chemistry databases

SwissLipidsiSLP:000001659

Names & Taxonomyi

Protein namesi
Recommended name:
N-fatty-acyl-amino acid synthase/hydrolase PM20D11 Publication (EC:3.5.1.-1 Publication, EC:4.3.-.-1 Publication)
Alternative name(s):
Peptidase M20 domain-containing protein 1Imported
Gene namesi
Name:Pm20d1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2442939 Pm20d1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi125H → A: Loss of N-fatty-acyl-amino acid synthase/hydrolase activity. 1 Publication1
Mutagenesisi127D → A: Loss of N-fatty-acyl-amino acid synthase/hydrolase activity. 1 Publication1
Mutagenesisi465H → A: Loss of N-fatty-acyl-amino acid synthase/hydrolase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000032192925 – 503N-fatty-acyl-amino acid synthase/hydrolase PM20D1Add BLAST479

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi72N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi443N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8C165
PaxDbiQ8C165
PeptideAtlasiQ8C165
PRIDEiQ8C165

PTM databases

CarbonylDBiQ8C165
iPTMnetiQ8C165
PhosphoSitePlusiQ8C165
SwissPalmiQ8C165

Expressioni

Tissue specificityi

Detected in blood (at protein level). Widely expressed with higher expression in kidney, liver and brown adipose tissues.1 Publication

Inductioni

Up-regulated in adipose tissue upon cold exposure.1 Publication

Gene expression databases

BgeeiENSMUSG00000042251 Expressed in 159 organ(s), highest expression level in liver
CleanExiMM_PM20D1
ExpressionAtlasiQ8C165 baseline and differential
GenevisibleiQ8C165 MM

Interactioni

Protein-protein interaction databases

IntActiQ8C165, 5 interactors
MINTiQ8C165
STRINGi10090.ENSMUSP00000046079

Structurei

3D structure databases

ProteinModelPortaliQ8C165
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20A family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2275 Eukaryota
COG0624 LUCA
GeneTreeiENSGT00880000137960
HOGENOMiHOG000245216
HOVERGENiHBG059923
InParanoidiQ8C165
KOiK13049
OMAiPGICIGN
OrthoDBiEOG091G0BP2
PhylomeDBiQ8C165
TreeFamiTF328688

Family and domain databases

InterProiView protein in InterPro
IPR036264 Bact_exopeptidase_dim_dom
IPR002933 Peptidase_M20
IPR011650 Peptidase_M20_dimer
PfamiView protein in Pfam
PF07687 M20_dimer, 1 hit
PF01546 Peptidase_M20, 1 hit
SUPFAMiSSF55031 SSF55031, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8C165-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAELLASLPA WAAVLLLFFA TVSGSTGPRS RENRGASRIP SQFSEEERVA
60 70 80 90 100
IKEALKGAIQ IPTVSFSHEE SNTTALAEFG EYIRKAFPTV FHSSLVQHEV
110 120 130 140 150
VAKYSHLFTI QGSDPSLQPY MLMAHIDVVP APEEGWEVPP FSGLERNGFI
160 170 180 190 200
YGRGALDNKN SVMAILHALE LLLIRNYSPK RSFFIALGHD EEVSGEKGAQ
210 220 230 240 250
KISALLQARG VQLAFLVDEG SFILEGFIPN LEKPVAMISV TEKGALDLML
260 270 280 290 300
QVNMTPGHSS APPKETSIGI LSAAVSRLEQ TPMPNMFGGG PLKKTMKLLA
310 320 330 340 350
NEFSFPINIV LRNLWLFHPI VSRIMERNPI TNALVRTTTA LTMFNAGIKV
360 370 380 390 400
NVIPPLAQAT INCRIHPSQT VHEVLELVKN TVADDRVQLH VLRSFEPLPI
410 420 430 440 450
SPSDDQAMGY QLLQETIRSV FPEVDIVVPG ICIANTDTRH YANITNGMYR
460 470 480 490 500
FNPLPLNPQD FSGVHGINEK VSVQNYQNQV KFIFEFIQNA DTYKEPVPHL

HEL
Length:503
Mass (Da):55,663
Last modified:March 1, 2003 - v1
Checksum:iCD6C87E276E77795
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F7B8J4F7B8J4_MOUSE
N-fatty-acyl-amino acid synthase/hy...
Pm20d1
99Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti373E → G in BAE25627 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028883 mRNA Translation: BAC26171.1
AK143953 mRNA Translation: BAE25627.1
BC025830 mRNA Translation: AAH25830.1
BC120725 mRNA Translation: AAI20726.1
BC125395 mRNA Translation: AAI25396.1
CCDSiCCDS15274.1
RefSeqiNP_835180.2, NM_178079.3
XP_006529401.1, XM_006529338.3
UniGeneiMm.23844

Genome annotation databases

EnsembliENSMUST00000048660; ENSMUSP00000046079; ENSMUSG00000042251
ENSMUST00000112393; ENSMUSP00000108012; ENSMUSG00000042251
GeneIDi212933
KEGGimmu:212933
UCSCiuc007cnt.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028883 mRNA Translation: BAC26171.1
AK143953 mRNA Translation: BAE25627.1
BC025830 mRNA Translation: AAH25830.1
BC120725 mRNA Translation: AAI20726.1
BC125395 mRNA Translation: AAI25396.1
CCDSiCCDS15274.1
RefSeqiNP_835180.2, NM_178079.3
XP_006529401.1, XM_006529338.3
UniGeneiMm.23844

3D structure databases

ProteinModelPortaliQ8C165
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8C165, 5 interactors
MINTiQ8C165
STRINGi10090.ENSMUSP00000046079

Chemistry databases

SwissLipidsiSLP:000001659

Protein family/group databases

MEROPSiM20.011

PTM databases

CarbonylDBiQ8C165
iPTMnetiQ8C165
PhosphoSitePlusiQ8C165
SwissPalmiQ8C165

Proteomic databases

MaxQBiQ8C165
PaxDbiQ8C165
PeptideAtlasiQ8C165
PRIDEiQ8C165

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048660; ENSMUSP00000046079; ENSMUSG00000042251
ENSMUST00000112393; ENSMUSP00000108012; ENSMUSG00000042251
GeneIDi212933
KEGGimmu:212933
UCSCiuc007cnt.2 mouse

Organism-specific databases

CTDi148811
MGIiMGI:2442939 Pm20d1

Phylogenomic databases

eggNOGiKOG2275 Eukaryota
COG0624 LUCA
GeneTreeiENSGT00880000137960
HOGENOMiHOG000245216
HOVERGENiHBG059923
InParanoidiQ8C165
KOiK13049
OMAiPGICIGN
OrthoDBiEOG091G0BP2
PhylomeDBiQ8C165
TreeFamiTF328688

Miscellaneous databases

PROiPR:Q8C165
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042251 Expressed in 159 organ(s), highest expression level in liver
CleanExiMM_PM20D1
ExpressionAtlasiQ8C165 baseline and differential
GenevisibleiQ8C165 MM

Family and domain databases

InterProiView protein in InterPro
IPR036264 Bact_exopeptidase_dim_dom
IPR002933 Peptidase_M20
IPR011650 Peptidase_M20_dimer
PfamiView protein in Pfam
PF07687 M20_dimer, 1 hit
PF01546 Peptidase_M20, 1 hit
SUPFAMiSSF55031 SSF55031, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiP20D1_MOUSE
AccessioniPrimary (citable) accession number: Q8C165
Secondary accession number(s): Q3UNX0, Q8R117
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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