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Protein

Rap guanine nucleotide exchange factor 5

Gene

Rapgef5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Rap guanine nucleotide exchange factor 5
Alternative name(s):
Guanine nucleotide exchange factor for Rap1
M-Ras-regulated Rap GEF
Short name:
MR-GEF
Gene namesi
Name:Rapgef5
Synonyms:Gfr, Kiaa0277, Mrgef
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2444365 Rapgef5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688741 – 814Rap guanine nucleotide exchange factor 5Add BLAST814

Proteomic databases

EPDiQ8C0Q9
PaxDbiQ8C0Q9
PeptideAtlasiQ8C0Q9
PRIDEiQ8C0Q9

PTM databases

iPTMnetiQ8C0Q9
PhosphoSitePlusiQ8C0Q9

Expressioni

Gene expression databases

BgeeiENSMUSG00000041992 Expressed in 216 organ(s), highest expression level in embryo
CleanExiMM_RAPGEF5
ExpressionAtlasiQ8C0Q9 baseline and differential
GenevisibleiQ8C0Q9 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105313

Structurei

3D structure databases

ProteinModelPortaliQ8C0Q9
SMRiQ8C0Q9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 118DEPPROSITE-ProRule annotationAdd BLAST76
Domaini301 – 434N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST134
Domaini578 – 813Ras-GEFPROSITE-ProRule annotationAdd BLAST236

Phylogenomic databases

eggNOGiKOG2378 Eukaryota
ENOG410XPX9 LUCA
GeneTreeiENSGT00880000137870
HOGENOMiHOG000230545
HOVERGENiHBG056985
InParanoidiQ8C0Q9
KOiK08019
OMAiEEIFCRV
OrthoDBiEOG091G0HKG
PhylomeDBiQ8C0Q9
TreeFamiTF313184

Family and domain databases

CDDicd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
Gene3Di1.10.10.10, 1 hit
1.10.840.10, 1 hit
InterProiView protein in InterPro
IPR000591 DEP_dom
IPR008937 Ras-like_GEF
IPR000651 Ras-like_Gua-exchang_fac_N
IPR019804 Ras_G-nucl-exch_fac_CS
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
IPR029071 Ubiquitin-like_domsf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR23113 PTHR23113, 1 hit
PfamiView protein in Pfam
PF00610 DEP, 1 hit
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
SMARTiView protein in SMART
SM00049 DEP, 1 hit
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF48366 SSF48366, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50186 DEP, 1 hit
PS00720 RASGEF, 1 hit
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8C0Q9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTTGDCQTLS RRISNPYLEH SPSQIYGENS SCAGRALRNI IILQAADLVK
60 70 80 90 100
DRVNLKGFYR RSCVGSELVD WLLEHCPFVQ CRSMAIGVWQ LLLDMGIMSS
110 120 130 140 150
VDQHLYFQDN YVFYQFSSDE CSYLYCEFER EEEWQKGVKL LLELVHLIPA
160 170 180 190 200
RAGICDLSHQ KTEDSEESSD EILARLTSAV QRELAAVIAL KARKSAIEQD
210 220 230 240 250
DENADKHVTV TEANNGPDPQ AGVMCKLQER DDIGRIELVH KLARENCQFL
260 270 280 290 300
QTEKKESEKL EQQDDEVTMV QVKEQGQSVL VLKKVASCGP APTSGSAEND
310 320 330 340 350
ARYVVVSGTP EKILEHLLND LHLAEVQHKE TETLLDDFLL TYTVFMTTDD
360 370 380 390 400
LCQALLRHYS AKKYQGEEEN SDVPCRKRKV LHLVSQWISL YKDWLHEDEH
410 420 430 440 450
SKMFLKTIYR NVLDDVYEYP ILEKELKEFQ KILGVYRRHT VDEYSPQKKN
460 470 480 490 500
KALFHQFSLK ENWLQHRGTV AETEEIFCHV YITEHSYISV KAKVSSTAQE
510 520 530 540 550
ILKVVAEKLQ RAEEDLALVA ITFSGEKHEF QPNDLAISKS LEASGRIYVY
560 570 580 590 600
RKDLADTLNP LAENEESQQR SMRILGMNTW DLALELMSFD WSLFNSIHEQ
610 620 630 640 650
ELIYFTFSRQ GNGENTVNLS LLLQRCNEVQ LWVATEILLC SQLGKRVQLV
660 670 680 690 700
KKFIKIAAHC KAQQNLNSFF AIVMGLNTAS VSRLSQTWEK IPGKFKKLFS
710 720 730 740 750
ELESLTDPSL NHKAYRDAFK KMKPPKIPFM PLLLKDVTFI HEGNKTFLDN
760 770 780 790 800
LVNFEKLHMI ADTVRTLRHC RTNQFGSDVS PKEQQELKSY VNHLYVIDSQ
810
QALFELSHRL EPRA
Note: No experimental confirmation available.
Length:814
Mass (Da):93,725
Last modified:June 20, 2003 - v2
Checksum:i2E30E9956D25F0E2
GO
Isoform 2 (identifier: Q8C0Q9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-202: Missing.
     203-220: NADKHVTVTEANNGPDPQ → MSWDCGFKYLFAFSPTLK

Note: No experimental confirmation available.
Show »
Length:612
Mass (Da):70,913
Checksum:iDBD6552DDFD392B6
GO
Isoform 3 (identifier: Q8C0Q9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-234: Missing.
     235-301: RIELVHKLAR...PTSGSAENDA → MGSSRLRVFD...DDSVDSLLSD

Show »
Length:580
Mass (Da):67,547
Checksum:iBB394A21B3C9267B
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1Y7VJI0A0A1Y7VJI0_MOUSE
Rap guanine nucleotide exchange fac...
Rapgef5
359Annotation score:

Sequence cautioni

The sequence BAC65516 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti410R → W in BAC26736 (PubMed:12693553).Curated1
Sequence conflicti513E → K in AAH46627 (PubMed:15489334).Curated1
Sequence conflicti646R → L in BAC26723 (PubMed:12693553).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0076191 – 234Missing in isoform 3. 2 PublicationsAdd BLAST234
Alternative sequenceiVSP_0076171 – 202Missing in isoform 2. 1 PublicationAdd BLAST202
Alternative sequenceiVSP_007618203 – 220NADKH…GPDPQ → MSWDCGFKYLFAFSPTLK in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_007620235 – 301RIELV…AENDA → MGSSRLRVFDPPLERKDSAA LSERQLPLPTFDVPYFKYID EEDEDDEWSSRSQSSTEDDS VDSLLSD in isoform 3. 2 PublicationsAdd BLAST67

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030016 mRNA Translation: BAC26736.1
AK029995 mRNA Translation: BAC26723.1
AK083591 mRNA Translation: BAC38963.1
AK122234 mRNA Translation: BAC65516.1 Different initiation.
BC046627 mRNA Translation: AAH46627.1
CCDSiCCDS36577.1 [Q8C0Q9-1]
RefSeqiNP_787126.3, NM_175930.5 [Q8C0Q9-1]
UniGeneiMm.227642

Genome annotation databases

EnsembliENSMUST00000109691; ENSMUSP00000105313; ENSMUSG00000041992 [Q8C0Q9-1]
ENSMUST00000222105; ENSMUSP00000152875; ENSMUSG00000041992 [Q8C0Q9-2]
ENSMUST00000222185; ENSMUSP00000152339; ENSMUSG00000041992 [Q8C0Q9-3]
GeneIDi217944
KEGGimmu:217944
UCSCiuc007pib.2 mouse [Q8C0Q9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030016 mRNA Translation: BAC26736.1
AK029995 mRNA Translation: BAC26723.1
AK083591 mRNA Translation: BAC38963.1
AK122234 mRNA Translation: BAC65516.1 Different initiation.
BC046627 mRNA Translation: AAH46627.1
CCDSiCCDS36577.1 [Q8C0Q9-1]
RefSeqiNP_787126.3, NM_175930.5 [Q8C0Q9-1]
UniGeneiMm.227642

3D structure databases

ProteinModelPortaliQ8C0Q9
SMRiQ8C0Q9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105313

PTM databases

iPTMnetiQ8C0Q9
PhosphoSitePlusiQ8C0Q9

Proteomic databases

EPDiQ8C0Q9
PaxDbiQ8C0Q9
PeptideAtlasiQ8C0Q9
PRIDEiQ8C0Q9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000109691; ENSMUSP00000105313; ENSMUSG00000041992 [Q8C0Q9-1]
ENSMUST00000222105; ENSMUSP00000152875; ENSMUSG00000041992 [Q8C0Q9-2]
ENSMUST00000222185; ENSMUSP00000152339; ENSMUSG00000041992 [Q8C0Q9-3]
GeneIDi217944
KEGGimmu:217944
UCSCiuc007pib.2 mouse [Q8C0Q9-1]

Organism-specific databases

CTDi9771
MGIiMGI:2444365 Rapgef5
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2378 Eukaryota
ENOG410XPX9 LUCA
GeneTreeiENSGT00880000137870
HOGENOMiHOG000230545
HOVERGENiHBG056985
InParanoidiQ8C0Q9
KOiK08019
OMAiEEIFCRV
OrthoDBiEOG091G0HKG
PhylomeDBiQ8C0Q9
TreeFamiTF313184

Miscellaneous databases

ChiTaRSiRapgef5 mouse
PROiPR:Q8C0Q9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041992 Expressed in 216 organ(s), highest expression level in embryo
CleanExiMM_RAPGEF5
ExpressionAtlasiQ8C0Q9 baseline and differential
GenevisibleiQ8C0Q9 MM

Family and domain databases

CDDicd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
Gene3Di1.10.10.10, 1 hit
1.10.840.10, 1 hit
InterProiView protein in InterPro
IPR000591 DEP_dom
IPR008937 Ras-like_GEF
IPR000651 Ras-like_Gua-exchang_fac_N
IPR019804 Ras_G-nucl-exch_fac_CS
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
IPR029071 Ubiquitin-like_domsf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR23113 PTHR23113, 1 hit
PfamiView protein in Pfam
PF00610 DEP, 1 hit
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
SMARTiView protein in SMART
SM00049 DEP, 1 hit
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF48366 SSF48366, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50186 DEP, 1 hit
PS00720 RASGEF, 1 hit
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPGF5_MOUSE
AccessioniPrimary (citable) accession number: Q8C0Q9
Secondary accession number(s): Q8BJJ9, Q8C0R5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: November 7, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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