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Protein

Isoaspartyl peptidase/L-asparaginase

Gene

Asrgl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.By similarity

Catalytic activityi

L-asparagine + H2O = L-aspartate + NH3.By similarity
Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei185NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease

Enzyme and pathway databases

ReactomeiR-MMU-71182 Phenylalanine and tyrosine catabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Isoaspartyl peptidase/L-asparaginase (EC:3.4.19.5By similarity, EC:3.5.1.1By similarity)
Alternative name(s):
Asparaginase-like protein 1
Beta-aspartyl-peptidase
Isoaspartyl dipeptidase
L-asparagine amidohydrolase
Cleaved into the following 2 chains:
Gene namesi
Name:Asrgl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1913764 Asrgl1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004205591 – 184Isoaspartyl peptidase/L-asparaginase alpha chainAdd BLAST184
ChainiPRO_0000420560185 – 326Isoaspartyl peptidase/L-asparaginase beta chainAdd BLAST142

Post-translational modificationi

Cleaved into an alpha and beta chain by autocatalysis; this activates the enzyme. The N-terminal residue of the beta subunit is responsible for the nucleophile hydrolase activity.By similarity

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

EPDiQ8C0M9
MaxQBiQ8C0M9
PaxDbiQ8C0M9
PeptideAtlasiQ8C0M9
PRIDEiQ8C0M9

2D gel databases

REPRODUCTION-2DPAGEiIPI00223875

PTM databases

iPTMnetiQ8C0M9
PhosphoSitePlusiQ8C0M9
SwissPalmiQ8C0M9

Expressioni

Gene expression databases

BgeeiENSMUSG00000024654
CleanExiMM_ASRGL1
GenevisibleiQ8C0M9 MM

Interactioni

Subunit structurei

Heterodimer of an alpha and beta chain produced by autocleavage. This heterodimer may then dimerize in turn, giving rise to a heterotetramer.By similarity

Protein-protein interaction databases

BioGridi211529, 1 interactor
IntActiQ8C0M9, 2 interactors
MINTiQ8C0M9
STRINGi10090.ENSMUSP00000051709

Structurei

3D structure databases

ProteinModelPortaliQ8C0M9
SMRiQ8C0M9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni213 – 216Substrate bindingBy similarity4
Regioni236 – 239Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the Ntn-hydrolase family.Curated

Phylogenomic databases

eggNOGiKOG1592 Eukaryota
COG1446 LUCA
GeneTreeiENSGT00530000063034
HOGENOMiHOG000174613
HOVERGENiHBG101662
InParanoidiQ8C0M9
KOiK13051
OMAiWAAAKDD
OrthoDBiEOG091G0CKQ
PhylomeDBiQ8C0M9
TreeFamiTF323960

Family and domain databases

CDDicd04702 ASRGL1_like, 1 hit
InterProiView protein in InterPro
IPR033844 ASRGL1_meta
IPR029055 Ntn_hydrolases_N
IPR000246 Peptidase_T2
PANTHERiPTHR10188 PTHR10188, 1 hit
PfamiView protein in Pfam
PF01112 Asparaginase_2, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8C0M9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACARGTVAP PVRASIDVSL VVVVHGGGAS NISANRKELV REGIARAATE
60 70 80 90 100
GYKILKAGGS AVDAVEGAVT VLENDPEFNA GYGSVLNVNG DIEMDASIMD
110 120 130 140 150
GKDLSAGAVS AVRCIANPVK LARLVMEKTP HCFLTGHGAE KFAEDMGIPQ
160 170 180 190 200
VPVEKLITER TKKHLEKEKL EKGAQNADCP KNSGTVGAVA LDCRGNLAYA
210 220 230 240 250
TSTGGIVNKM VGRVGDSPCI GAGGYADNNL GAVSTTGHGE SILKVNLARL
260 270 280 290 300
ALFHVEQGKT VEEAAQLALD YMKSKLKGLG GLILVNKTGD WVAKWTSASM
310 320
PWAAVKNGKL QAGIDLCETR TRDLPC
Length:326
Mass (Da):33,950
Last modified:March 1, 2003 - v1
Checksum:iB5AC25922D9A1338
GO

Sequence cautioni

The sequence AAH16106 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB24431 differs from that shown. Reason: Frameshift at position 29.Curated
The sequence BAC25294 differs from that shown. Reason: Frameshift at positions 24 and 29.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006147 mRNA Translation: BAB24431.1 Frameshift.
AK010381 mRNA Translation: BAC25294.1 Frameshift.
AK030209 mRNA Translation: BAC26844.1
BC016106 mRNA Translation: AAH16106.1 Different initiation.
CCDSiCCDS29566.1
RefSeqiNP_079886.2, NM_025610.3
UniGeneiMm.272847

Genome annotation databases

EnsembliENSMUST00000049948; ENSMUSP00000051709; ENSMUSG00000024654
GeneIDi66514
KEGGimmu:66514
UCSCiuc008gon.1 mouse

Similar proteinsi

Entry informationi

Entry nameiASGL1_MOUSE
AccessioniPrimary (citable) accession number: Q8C0M9
Secondary accession number(s): Q91WC8, Q9CVX3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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