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Protein

Autophagy-related protein 16-1

Gene

Atg16l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:18849966, PubMed:12665549, PubMed:24954904, PubMed:24553140, PubMed:23392225). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (By similarity). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340). Instead, promotes with NOD2 an autophagy-dependent antibacterial pathway. Plays a role in regulating morphology and function of Paneth cell.By similarity6 Publications

GO - Molecular functioni

  • GTPase binding Source: MGI
  • identical protein binding Source: IntAct

GO - Biological processi

  • autophagosome assembly Source: MGI
  • negative stranded viral RNA replication Source: MGI
  • protein lipidation involved in autophagosome assembly Source: MGI
  • protein localization to phagophore assembly site Source: MGI
  • protein transport Source: UniProtKB-KW
  • xenophagy Source: MGI

Keywordsi

Biological processAutophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 16-1
Alternative name(s):
APG16-like 1
Gene namesi
Name:Atg16l1
Synonyms:Apg16l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1924290 Atg16l1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi299D → E: Prevents cleavage by activated CASP3. 1 Publication1
Mutagenesisi300T → A: No effect on the stability of the protein under normal conditions. Enhances cleavage and degradation mediated by activated CASP3 and CASP7. Results in reduced autophagy and defective clearance of intestinal pathogens. Increases secretion of proinflammatory cytokine IL1B and type I IFN. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508491 – 607Autophagy-related protein 16-1Add BLAST607

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei139PhosphoserineBy similarity1
Modified residuei269PhosphoserineCombined sources1
Modified residuei287PhosphoserineCombined sources1

Post-translational modificationi

Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.1 Publication
Phosphorylation at Ser-139 promotes association with the ATG12-ATG5 conjugate to form the ATG12-ATG5-ATG16L1 complex.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8C0J2
PeptideAtlasiQ8C0J2
PRIDEiQ8C0J2

2D gel databases

REPRODUCTION-2DPAGEiQ8C0J2

PTM databases

iPTMnetiQ8C0J2
PhosphoSitePlusiQ8C0J2

Expressioni

Tissue specificityi

Widely expressed. Isoform 1: Expressed in liver. Isoform 2: Highly expressed in liver. Isoform 3: Expressed in brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000026289 Expressed in 274 organ(s), highest expression level in primary oocyte
CleanExiMM_ATG16L1
ExpressionAtlasiQ8C0J2 baseline and differential
GenevisibleiQ8C0J2 MM

Interactioni

Subunit structurei

Homodimer (By similarity). Homooligomer (PubMed:12665549). Interacts with WIPI2 (PubMed:24954904). Interacts with RB1CC1; the interaction is required for ULK1 complex-dependent autophagy (PubMed:23392225, PubMed:23262492). Interacts with ATG5 (PubMed:12665549). Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Interacts with RAB33B (PubMed:18448665). Interacts (via WD repeats) with TMEM59; the interaction mediates unconventional autophagic activity of TMEM59 (By similarity). Interacts with TLR2 and NOD2 (By similarity). Interacts (via WD repeats) with MEFV (By similarity). Interacts (via N-terminal) with CLTC (By similarity). Interacts with NOD2 (By similarity). Interacts with TUFM (By similarity).By similarity5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi218476, 7 interactors
ComplexPortaliCPX-328 Atg12-Atg5-Atg16l1 complex
CORUMiQ8C0J2
DIPiDIP-31966N
IntActiQ8C0J2, 15 interactors
MINTiQ8C0J2
STRINGi10090.ENSMUSP00000108815

Structurei

3D structure databases

ProteinModelPortaliQ8C0J2
SMRiQ8C0J2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati320 – 359WD 1PROSITE-ProRule annotationAdd BLAST40
Repeati364 – 403WD 2PROSITE-ProRule annotationAdd BLAST40
Repeati406 – 445WD 3PROSITE-ProRule annotationAdd BLAST40
Repeati447 – 484WD 4PROSITE-ProRule annotationAdd BLAST38
Repeati486 – 525WD 5PROSITE-ProRule annotationAdd BLAST40
Repeati532 – 573WD 6PROSITE-ProRule annotationAdd BLAST42
Repeati575 – 607WD 7PROSITE-ProRule annotationAdd BLAST33

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 43Interaction with ATG5By similarityAdd BLAST31
Regioni207 – 230WIPI2-bindingBy similarityAdd BLAST24
Regioni230 – 242RB1CC1-binding1 PublicationAdd BLAST13

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili79 – 230Sequence analysisAdd BLAST152

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi296 – 299Caspase cleavage4

Sequence similaritiesi

Belongs to the WD repeat ATG16 family.Curated

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0288 Eukaryota
ENOG410XQYA LUCA
GeneTreeiENSGT00910000144058
HOVERGENiHBG050534
InParanoidiQ8C0J2
KOiK17890
OMAiNAVKQTF
PhylomeDBiQ8C0J2
TreeFamiTF315541

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR013923 Autophagy-rel_prot_16
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF08614 ATG16, 1 hit
PF00400 WD40, 4 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 3 hits
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8C0J2-1) [UniParc]FASTAAdd to basket
Also known as: Apg16Lbeta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSSGLRAADF PRWKRHIAEE LRRRDRLQRQ AFEEIILQYT KLLEKSDLHS
60 70 80 90 100
VLTQKLQAEK HDMPNRHEIS PGHDGAWNDS QLQEMAQLRI KHQEELTELH
110 120 130 140 150
KKRGELAQLV IDLNNQMQQK DKEIQMNEAK ISEYLQTISD LETNCLDLRT
160 170 180 190 200
KLQDLEVANQ TLKDEYDALQ ITFTALEEKL RKTTEENQEL VTRWMAEKAQ
210 220 230 240 250
EANRLNAENE KDSRRRQARL QKELAEAAKE PLPVEQDDDI EVIVDETSDH
260 270 280 290 300
TEETSPVRAV SRAATKRLSQ PAGGLLDSIT NIFGRRSVSS IPVPQDIMDT
310 320 330 340 350
HPASGKDVRV PTTASYVFDA HDGEVNAVQF SPGSRLLATG GMDRRVKLWE
360 370 380 390 400
AFGDKCEFKG SLSGSNAGIT SIEFDSAGAY LLAASNDFAS RIWTVDDYRL
410 420 430 440 450
RHTLTGHSGK VLSAKFLLDN ARIVSGSHDR TLKLWDLRSK VCIKTVFAGS
460 470 480 490 500
SCNDIVCTEQ CVMSGHFDKK IRFWDIRSES VVREMELLGK ITALDLNPER
510 520 530 540 550
TELLSCSRDD LLKVIDLRTN AVKQTFSAPG FKCGSDWTRV VFSPDGSYVA
560 570 580 590 600
AGSAEGSLYV WSVLTGKVEK VLSKQHSSSI NAVAWAPSGL HVVSVDKGSR

AVLWAQP
Length:607
Mass (Da):68,172
Last modified:March 1, 2003 - v1
Checksum:i63920320883A69DF
GO
Isoform 2 (identifier: Q8C0J2-2) [UniParc]FASTAAdd to basket
Also known as: Apg16Lalpha

The sequence of this isoform differs from the canonical sequence as follows:
     266-284: Missing.

Note: No experimental confirmation available.
Show »
Length:588
Mass (Da):66,203
Checksum:iCE2AB829A7911274
GO
Isoform 3 (identifier: Q8C0J2-3) [UniParc]FASTAAdd to basket
Also known as: Apg16Lgamma

The sequence of this isoform differs from the canonical sequence as follows:
     284-284: G → GLSESPLLGHHSSDAAR

Show »
Length:623
Mass (Da):69,831
Checksum:i823453BB5D7DE6FE
GO
Isoform 4 (identifier: Q8C0J2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     284-284: G → GLSESPLLGHHSSDAAR
     443-452: IKTVFAGSSC → EEMQSLCVFM
     453-607: Missing.

Note: No experimental confirmation available.
Show »
Length:468
Mass (Da):53,024
Checksum:iFFB7AC4AE34DF8F7
GO
Isoform 5 (identifier: Q8C0J2-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     266-284: Missing.
     442-442: C → CEEMQSLCVLMVFGFLSG

Note: No experimental confirmation available.
Show »
Length:605
Mass (Da):68,075
Checksum:iB0BB8DE0748401B6
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YZW7D3YZW7_MOUSE
Autophagy-related protein 16-1
Atg16l1
154Annotation score:

Sequence cautioni

The sequence BAD21370 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83Q → H in BAC55090 (PubMed:12665549).Curated1
Sequence conflicti83Q → H in BAC55091 (PubMed:12665549).Curated1
Sequence conflicti83Q → H in BAC55092 (PubMed:12665549).Curated1
Sequence conflicti83Q → H in BAB23866 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013391266 – 284Missing in isoform 2 and isoform 5. 2 PublicationsAdd BLAST19
Alternative sequenceiVSP_013392284G → GLSESPLLGHHSSDAAR in isoform 3 and isoform 4. 3 Publications1
Alternative sequenceiVSP_013393442C → CEEMQSLCVLMVFGFLSG in isoform 5. 1 Publication1
Alternative sequenceiVSP_013394443 – 452IKTVFAGSSC → EEMQSLCVFM in isoform 4. 1 Publication10
Alternative sequenceiVSP_013395453 – 607Missing in isoform 4. 1 PublicationAdd BLAST155

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB087879 mRNA Translation: BAC55090.1
AB087880 mRNA Translation: BAC55091.1
AB087881 mRNA Translation: BAC55092.1
AK005181 mRNA Translation: BAB23866.1
AK030983 mRNA Translation: BAC27201.1
AK131120 Transcribed RNA Translation: BAD21370.1 Different initiation.
BC049122 mRNA Translation: AAH49122.1
CCDSiCCDS15136.1 [Q8C0J2-1]
CCDS56637.1 [Q8C0J2-3]
CCDS56638.1 [Q8C0J2-2]
RefSeqiNP_001192320.1, NM_001205391.1 [Q8C0J2-3]
NP_084122.2, NM_029846.4 [Q8C0J2-1]
UniGeneiMm.272972
Mm.456099

Genome annotation databases

EnsembliENSMUST00000027512; ENSMUSP00000027512; ENSMUSG00000026289 [Q8C0J2-1]
ENSMUST00000113186; ENSMUSP00000108811; ENSMUSG00000026289 [Q8C0J2-2]
ENSMUST00000113190; ENSMUSP00000108815; ENSMUSG00000026289 [Q8C0J2-3]
GeneIDi77040
KEGGimmu:77040
UCSCiuc007bxl.2 mouse [Q8C0J2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB087879 mRNA Translation: BAC55090.1
AB087880 mRNA Translation: BAC55091.1
AB087881 mRNA Translation: BAC55092.1
AK005181 mRNA Translation: BAB23866.1
AK030983 mRNA Translation: BAC27201.1
AK131120 Transcribed RNA Translation: BAD21370.1 Different initiation.
BC049122 mRNA Translation: AAH49122.1
CCDSiCCDS15136.1 [Q8C0J2-1]
CCDS56637.1 [Q8C0J2-3]
CCDS56638.1 [Q8C0J2-2]
RefSeqiNP_001192320.1, NM_001205391.1 [Q8C0J2-3]
NP_084122.2, NM_029846.4 [Q8C0J2-1]
UniGeneiMm.272972
Mm.456099

3D structure databases

ProteinModelPortaliQ8C0J2
SMRiQ8C0J2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218476, 7 interactors
ComplexPortaliCPX-328 Atg12-Atg5-Atg16l1 complex
CORUMiQ8C0J2
DIPiDIP-31966N
IntActiQ8C0J2, 15 interactors
MINTiQ8C0J2
STRINGi10090.ENSMUSP00000108815

PTM databases

iPTMnetiQ8C0J2
PhosphoSitePlusiQ8C0J2

2D gel databases

REPRODUCTION-2DPAGEiQ8C0J2

Proteomic databases

PaxDbiQ8C0J2
PeptideAtlasiQ8C0J2
PRIDEiQ8C0J2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027512; ENSMUSP00000027512; ENSMUSG00000026289 [Q8C0J2-1]
ENSMUST00000113186; ENSMUSP00000108811; ENSMUSG00000026289 [Q8C0J2-2]
ENSMUST00000113190; ENSMUSP00000108815; ENSMUSG00000026289 [Q8C0J2-3]
GeneIDi77040
KEGGimmu:77040
UCSCiuc007bxl.2 mouse [Q8C0J2-1]

Organism-specific databases

CTDi55054
MGIiMGI:1924290 Atg16l1

Phylogenomic databases

eggNOGiKOG0288 Eukaryota
ENOG410XQYA LUCA
GeneTreeiENSGT00910000144058
HOVERGENiHBG050534
InParanoidiQ8C0J2
KOiK17890
OMAiNAVKQTF
PhylomeDBiQ8C0J2
TreeFamiTF315541

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy

Miscellaneous databases

PROiPR:Q8C0J2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026289 Expressed in 274 organ(s), highest expression level in primary oocyte
CleanExiMM_ATG16L1
ExpressionAtlasiQ8C0J2 baseline and differential
GenevisibleiQ8C0J2 MM

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR013923 Autophagy-rel_prot_16
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF08614 ATG16, 1 hit
PF00400 WD40, 4 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 3 hits
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiA16L1_MOUSE
AccessioniPrimary (citable) accession number: Q8C0J2
Secondary accession number(s): Q6KAT7
, Q80U97, Q80U98, Q80U99, Q80Y53, Q9DB63
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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