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Protein

Vacuolar protein sorting-associated protein 26B

Gene

Vps26b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the retromer cargo-selective complex (CSC) (PubMed:21040701, PubMed:21920005). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (By similarity). May be involved in retrograde transport of SORT1 but not of IGF2R (PubMed:21040701). Acts redundantly with VSP26A in SNX-27 mediated endocytic recycling of SLC2A1/GLUT1 (PubMed:25136126).By similarity3 Publications

GO - Molecular functioni

  • protein transporter activity Source: GO_Central

GO - Biological processi

  • cellular response to interferon-gamma Source: MGI
  • intracellular protein transport Source: GO_Central
  • retrograde transport, endosome to Golgi Source: UniProtKB

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 26B
Alternative name(s):
Vesicle protein sorting 26B
Gene namesi
Name:Vps26b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1917656 Vps26b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42D → A: Disrupts interaction with SNX27. 1 Publication1
Mutagenesisi152L → A: Disrupts interaction with SNX27. 1 Publication1
Mutagenesisi197L → S: No endosomal localization; no effect on interaction with VPS35:VPS29 dimer; when associated with E-199. 1 Publication1
Mutagenesisi199R → E: No endosomal localization; no effect on interaction with VPS35:VPS29 dimer; when associated with S-197. 1 Publication1
Mutagenesisi233 – 234IM → DN: Disrupts interaction with VPS35:VPS29 dimer; no endosomal localization. 1 Publication2
Mutagenesisi240 – 242RGE → SAS: No endosomal localization; no effect on interaction with VPS35:VPS29 dimer. 1 Publication3
Mutagenesisi245P → S: Disrupts interaction with VPS35:VPS29 dimer; no endosomal localization; when associated with S-247. 1 Publication1
Mutagenesisi247R → S: Disrupts interaction with VPS35:VPS29 dimer; no endosomal localization; when associated with S-245. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002470901 – 336Vacuolar protein sorting-associated protein 26BAdd BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei302PhosphoserineCombined sources1
Modified residuei304PhosphoserineCombined sources1
Modified residuei319PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8C0E2
PaxDbiQ8C0E2
PeptideAtlasiQ8C0E2
PRIDEiQ8C0E2

PTM databases

iPTMnetiQ8C0E2
PhosphoSitePlusiQ8C0E2

Expressioni

Tissue specificityi

Ubiquitously expressed in developing embryo and adult. Highly expressed in brain.2 Publications

Gene expression databases

BgeeiENSMUSG00000031988 Expressed in 303 organ(s), highest expression level in cardiac ventricle
CleanExiMM_VPS26B
GenevisibleiQ8C0E2 MM

Interactioni

Subunit structurei

Component of the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS), formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35 (PubMed:21040701, PubMed:21920005, PubMed:18088321, PubMed:20875039). The CSC has a highly elongated structure with VPS26 and VPS29 binding independently at opposite distal ends of VPS35 as central platform (Probable). The CSC is believed to associate with variable sorting nexins to form functionally distinct retromer complex variants. The originally described SNX-BAR retromer is a pentamer containing the CSC and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity. The CSC associates with SNX3 to form a SNX3-retromer complex. The CSC associates with SNX27, the WASH complex and the SNX-BAR subcomplex to form the SNX27-retromer complex (By similarity). Interacts with VPS29, VPS35, TBC1D5, GOLPH3, SNX27 (PubMed:16190980, PubMed:21040701, PubMed:21920005, PubMed:25136126).1 PublicationBy similarity6 Publications

Protein-protein interaction databases

BioGridi213223, 3 interactors
IntActiQ8C0E2, 6 interactors
MINTiQ8C0E2
STRINGi10090.ENSMUSP00000034470

Structurei

Secondary structure

1336
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8C0E2
SMRiQ8C0E2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8C0E2

Family & Domainsi

Sequence similaritiesi

Belongs to the VPS26 family.Curated

Phylogenomic databases

eggNOGiKOG3063 Eukaryota
ENOG410XSI1 LUCA
GeneTreeiENSGT00390000002588
HOGENOMiHOG000191799
HOVERGENiHBG082914
InParanoidiQ8C0E2
KOiK18466
OMAiGEMRQAQ
OrthoDBiEOG091G09TQ
PhylomeDBiQ8C0E2
TreeFamiTF300907

Family and domain databases

Gene3Di2.60.40.640, 2 hits
InterProiView protein in InterPro
IPR014752 Arrestin_C
IPR028934 Vps26-related
PANTHERiPTHR12233 PTHR12233, 1 hit
PfamiView protein in Pfam
PF03643 Vps26, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q8C0E2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSFFGFGQSV EVEILLNDAE SRKRAEHKTE DGKKEKYFLF YDGETVSGKV
60 70 80 90 100
SLSLKNPNKR LEHQGIKIEF IGQIELYYDR GNHHEFVSLV KDLARPGEIT
110 120 130 140 150
QSQAFDFEFT HVEKPYESYT GQNVKLRYFL RATISRRLND VVKEMDIVVH
160 170 180 190 200
TLSTYPELNS SIKMEVGIED CLHIEFEYNK SKYHLKDVIV GKIYFLLVRI
210 220 230 240 250
KIKHMEIDII KRETTGTGPN VYHENDTIAK YEIMDGAPVR GESIPIRLFL
260 270 280 290 300
AGYELTPTMR DINKKFSVRY YLNLVLIDEE ERRYFKQQEV VLWRKGDIVR
310 320 330
KSMSHQAAIA SQRFEGTTSL GEVRTPGQLS DNNSRQ
Length:336
Mass (Da):39,125
Last modified:March 1, 2003 - v1
Checksum:iAD5395AF30900018
GO
Isoform 2 (identifier: Q8C0E2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     243-336: SIPIRLFLAG...GQLSDNNSRQ → PPGLGPLSLC...LFYFIYLFIF

Note: No experimental confirmation available.
Show »
Length:282
Mass (Da):32,465
Checksum:i6ACC5EFA4E20BD3B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019927243 – 336SIPIR…NNSRQ → PPGLGPLSLCSQTTCSVSAL GKGSNFEVKSLFYFIYLFIF in isoform 2. 1 PublicationAdd BLAST94

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031589 mRNA Translation: BAC27465.1
AK150929 mRNA Translation: BAE29967.1
AK155578 mRNA Translation: BAE33333.1
AK165539 mRNA Translation: BAE38244.1
AK171154 mRNA Translation: BAE42279.1
BC029758 mRNA Translation: AAH29758.1
BC049180 mRNA Translation: AAH49180.1
BC052721 mRNA Translation: AAH52721.1
BC062972 mRNA Translation: AAH62972.1
CCDSiCCDS22938.1 [Q8C0E2-1]
RefSeqiNP_821170.1, NM_178027.4 [Q8C0E2-1]
UniGeneiMm.218717
Mm.440390

Genome annotation databases

EnsembliENSMUST00000034470; ENSMUSP00000034470; ENSMUSG00000031988 [Q8C0E2-1]
GeneIDi69091
KEGGimmu:69091
UCSCiuc009oqd.2 mouse [Q8C0E2-1]
uc009oqf.2 mouse [Q8C0E2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031589 mRNA Translation: BAC27465.1
AK150929 mRNA Translation: BAE29967.1
AK155578 mRNA Translation: BAE33333.1
AK165539 mRNA Translation: BAE38244.1
AK171154 mRNA Translation: BAE42279.1
BC029758 mRNA Translation: AAH29758.1
BC049180 mRNA Translation: AAH49180.1
BC052721 mRNA Translation: AAH52721.1
BC062972 mRNA Translation: AAH62972.1
CCDSiCCDS22938.1 [Q8C0E2-1]
RefSeqiNP_821170.1, NM_178027.4 [Q8C0E2-1]
UniGeneiMm.218717
Mm.440390

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2R51X-ray2.10A7-336[»]
3LH8X-ray2.60A/B7-336[»]
3LH9X-ray2.40A/B7-336[»]
3LHAX-ray2.80A/B7-336[»]
ProteinModelPortaliQ8C0E2
SMRiQ8C0E2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213223, 3 interactors
IntActiQ8C0E2, 6 interactors
MINTiQ8C0E2
STRINGi10090.ENSMUSP00000034470

PTM databases

iPTMnetiQ8C0E2
PhosphoSitePlusiQ8C0E2

Proteomic databases

EPDiQ8C0E2
PaxDbiQ8C0E2
PeptideAtlasiQ8C0E2
PRIDEiQ8C0E2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034470; ENSMUSP00000034470; ENSMUSG00000031988 [Q8C0E2-1]
GeneIDi69091
KEGGimmu:69091
UCSCiuc009oqd.2 mouse [Q8C0E2-1]
uc009oqf.2 mouse [Q8C0E2-2]

Organism-specific databases

CTDi112936
MGIiMGI:1917656 Vps26b

Phylogenomic databases

eggNOGiKOG3063 Eukaryota
ENOG410XSI1 LUCA
GeneTreeiENSGT00390000002588
HOGENOMiHOG000191799
HOVERGENiHBG082914
InParanoidiQ8C0E2
KOiK18466
OMAiGEMRQAQ
OrthoDBiEOG091G09TQ
PhylomeDBiQ8C0E2
TreeFamiTF300907

Miscellaneous databases

ChiTaRSiVps26b mouse
EvolutionaryTraceiQ8C0E2
PROiPR:Q8C0E2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031988 Expressed in 303 organ(s), highest expression level in cardiac ventricle
CleanExiMM_VPS26B
GenevisibleiQ8C0E2 MM

Family and domain databases

Gene3Di2.60.40.640, 2 hits
InterProiView protein in InterPro
IPR014752 Arrestin_C
IPR028934 Vps26-related
PANTHERiPTHR12233 PTHR12233, 1 hit
PfamiView protein in Pfam
PF03643 Vps26, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVP26B_MOUSE
AccessioniPrimary (citable) accession number: Q8C0E2
Secondary accession number(s): Q3TN45, Q8K2V3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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