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Protein

Centrosomal protein of 68 kDa

Gene

Cep68

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in maintenance of centrosome cohesion, probably as part of a linker structure which prevents centrosome splitting. Required for localization of CDK5RAP2 to the centrosome during interphase.By similarity

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Centrosomal protein of 68 kDa
Short name:
Cep68
Gene namesi
Name:Cep68
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2667663 Cep68

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000894951 – 733Centrosomal protein of 68 kDaAdd BLAST733

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei315PhosphoserineBy similarity1
Modified residuei453PhosphoserineCombined sources1
Modified residuei459PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation by PLK1 is required for binding to BTRC in prometaphase. Phosphorylated directly or indirectly by NEK2. NEK2-mediated phosphorylation promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis.By similarity
Ubiquitinated and targeted for proteasomal degradation in early mitosis by the SCF(BTRC) and/or SCF(FBXW11) E3 ubiquitin-protein ligase complexes. Degradation is complete by prometaphase and is required for removal of CDK5RAP2 from the peripheral pericentriolar material and subsequent centriole separation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8C0D9
PeptideAtlasiQ8C0D9
PRIDEiQ8C0D9

PTM databases

iPTMnetiQ8C0D9
PhosphoSitePlusiQ8C0D9

Expressioni

Gene expression databases

BgeeiENSMUSG00000044066 Expressed in 266 organ(s), highest expression level in brain blood vessel
CleanExiMM_CEP68
ExpressionAtlasiQ8C0D9 baseline and differential
GenevisibleiQ8C0D9 MM

Interactioni

Subunit structurei

Interacts with CNTLN; the interaction recruits CEP68 to the centrosome. Interacts with the SCF(FBXW11) complex which contains SKP1, CUL1 and FBXW11; the interaction is probably mediated by FBXW11 and the complex also contains CDK5RAP2 and PCNT. Also interacts with F-box protein BTRC. Interacts with serine/threonine-protein kinase PLK1; the interaction leads to phosphorylation of CEP68 and its subsequent degradation. Interacts with NEK2; the interaction leads to phosphorylation of CEP68.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054943

Structurei

3D structure databases

ProteinModelPortaliQ8C0D9
SMRiQ8C0D9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG410IIDK Eukaryota
ENOG41129RE LUCA
GeneTreeiENSGT00810000125473
HOGENOMiHOG000070069
HOVERGENiHBG050898
InParanoidiQ8C0D9
KOiK16764
OMAiRRSPLWN
OrthoDBiEOG091G0T7P
PhylomeDBiQ8C0D9
TreeFamiTF333570

Family and domain databases

InterProiView protein in InterPro
IPR026696 AKAP6/CEP68
PANTHERiPTHR14514 PTHR14514, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8C0D9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MALSEDEAEA EVSVNTKVPS CGRWNSGKLL PSGLEPDQPL HLGVEGGPLW
60 70 80 90 100
RAEADPGCIS GVFLSRVHTA SKEPVADRSK PPLRGPLPSA SVGTGEVLHS
110 120 130 140 150
MGSQMEEDRL PASQDLLPAL QVFGTITVCS GQEADSEDFQ ATLDPSQVLG
160 170 180 190 200
LSQQPHTSGL PLPPQWKSTV SPGAPQLSSR SISASSVGSS LQDHQEKAGP
210 220 230 240 250
QRASFANVSS PELTVPQAAH SVVGAGPPLQ GSAQPLTSGS DATGLGKRHL
260 270 280 290 300
SFQAEYWACA LPNSLPPSPN RHSALWDPNK EYEDLLDYTY PLRPGPQLPK
310 320 330 340 350
QPESHVLTEP VLQDSGVDLD SLSVSPASTL KSPTNVSHNC SSAEVPTLPF
360 370 380 390 400
SGARESCLKR WPLGIFQKQG GTSLSSWNQL ASTPRAPGTE DASWENREAA
410 420 430 440 450
LRGTAEDCLP IGEDLRMGSP QLKTKEKEPP FPRQKRGRQH VSCPACVTPG
460 470 480 490 500
WPSEEEVGSD EEYLALPTRL TQVSSLVSYS GARPSFVNLH TGAAEEHSSL
510 520 530 540 550
QVSDSDKPAS PTLDSSHRKH PSGTSFQGPV GQNPCFRHSI QPQDSRGKSS
560 570 580 590 600
LMSNQTLGVS SKPLKTQPAS KAMTDRRLFS ELVAGETLPR TTDEQEKASL
610 620 630 640 650
VQCVQTFCCR LEELICWLYN VTDVADLSAP PRTSLTGLKS SLQLYRQFKK
660 670 680 690 700
DVDEHQSLTE SVLEKGEILL QCLLDNTPVL KDVLERIAKQ SGELESRADH
710 720 730
LYDSILASLD MLAGCTLIPD NRPTAAEHPH EGL
Length:733
Mass (Da):78,749
Last modified:April 26, 2005 - v2
Checksum:i15D93FCECDDC92A9
GO
Isoform 2 (identifier: Q8C0D9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     647-733: QFKKDVDEHQ...TAAEHPHEGL → VICSPGLFIHGDGS

Note: No experimental confirmation available.
Show »
Length:660
Mass (Da):70,487
Checksum:i4076BCB810C66E65
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3TRP8Q3TRP8_MOUSE
Centrosomal protein of 68 kDa
Cep68
633Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti228P → Q in BAC27484 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013477647 – 733QFKKD…PHEGL → VICSPGLFIHGDGS in isoform 2. 1 PublicationAdd BLAST87

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031622 mRNA Translation: BAC27484.1
AK140820 mRNA Translation: BAE24488.1
AL606522 Genomic DNA No translation available.
BC027174 mRNA Translation: AAH27174.1
CCDSiCCDS24456.1 [Q8C0D9-1]
RefSeqiNP_758464.2, NM_172260.3 [Q8C0D9-1]
UniGeneiMm.260314

Genome annotation databases

EnsembliENSMUST00000050611; ENSMUSP00000054943; ENSMUSG00000044066 [Q8C0D9-1]
ENSMUST00000109596; ENSMUSP00000105225; ENSMUSG00000044066 [Q8C0D9-2]
GeneIDi216543
KEGGimmu:216543
UCSCiuc007icw.1 mouse [Q8C0D9-1]
uc007icx.1 mouse [Q8C0D9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031622 mRNA Translation: BAC27484.1
AK140820 mRNA Translation: BAE24488.1
AL606522 Genomic DNA No translation available.
BC027174 mRNA Translation: AAH27174.1
CCDSiCCDS24456.1 [Q8C0D9-1]
RefSeqiNP_758464.2, NM_172260.3 [Q8C0D9-1]
UniGeneiMm.260314

3D structure databases

ProteinModelPortaliQ8C0D9
SMRiQ8C0D9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054943

PTM databases

iPTMnetiQ8C0D9
PhosphoSitePlusiQ8C0D9

Proteomic databases

PaxDbiQ8C0D9
PeptideAtlasiQ8C0D9
PRIDEiQ8C0D9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050611; ENSMUSP00000054943; ENSMUSG00000044066 [Q8C0D9-1]
ENSMUST00000109596; ENSMUSP00000105225; ENSMUSG00000044066 [Q8C0D9-2]
GeneIDi216543
KEGGimmu:216543
UCSCiuc007icw.1 mouse [Q8C0D9-1]
uc007icx.1 mouse [Q8C0D9-2]

Organism-specific databases

CTDi23177
MGIiMGI:2667663 Cep68

Phylogenomic databases

eggNOGiENOG410IIDK Eukaryota
ENOG41129RE LUCA
GeneTreeiENSGT00810000125473
HOGENOMiHOG000070069
HOVERGENiHBG050898
InParanoidiQ8C0D9
KOiK16764
OMAiRRSPLWN
OrthoDBiEOG091G0T7P
PhylomeDBiQ8C0D9
TreeFamiTF333570

Miscellaneous databases

PROiPR:Q8C0D9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000044066 Expressed in 266 organ(s), highest expression level in brain blood vessel
CleanExiMM_CEP68
ExpressionAtlasiQ8C0D9 baseline and differential
GenevisibleiQ8C0D9 MM

Family and domain databases

InterProiView protein in InterPro
IPR026696 AKAP6/CEP68
PANTHERiPTHR14514 PTHR14514, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCEP68_MOUSE
AccessioniPrimary (citable) accession number: Q8C0D9
Secondary accession number(s): Q3US49, Q5SW85, Q8R2V0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: November 7, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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