UniProtKB - Q8C0C7 (SYFA_MOUSE)
Protein
Phenylalanine--tRNA ligase alpha subunit
Gene
Farsa
Organism
Mus musculus (Mouse)
Status
Functioni
Catalytic activityi
- EC:6.1.1.20By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 329 | L-phenylalanineBy similarity | 1 | |
Binding sitei | 435 | L-phenylalanineBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- phenylalanine-tRNA ligase activity Source: UniProtKB
- tRNA binding Source: InterPro
GO - Biological processi
- phenylalanyl-tRNA aminoacylation Source: UniProtKB
- protein heterotetramerization Source: UniProtKB
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Phenylalanine--tRNA ligase alpha subunit (EC:6.1.1.20By similarity)Alternative name(s): Phenylalanyl-tRNA synthetase alpha subunit Short name: PheRS |
Gene namesi | Name:Farsa Synonyms:Farsla |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913840, Farsa |
Subcellular locationi
Other locations
- Cytoplasm By similarity
Cytosol
- cytosol Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB
- phenylalanine-tRNA ligase complex Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000280447 | 2 – 508 | Phenylalanine--tRNA ligase alpha subunitAdd BLAST | 507 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 193 | PhosphoserineBy similarity | 1 | |
Modified residuei | 301 | PhosphoserineCombined sources | 1 | |
Modified residuei | 311 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8C0C7 |
jPOSTi | Q8C0C7 |
MaxQBi | Q8C0C7 |
PaxDbi | Q8C0C7 |
PeptideAtlasi | Q8C0C7 |
PRIDEi | Q8C0C7 |
PTM databases
iPTMneti | Q8C0C7 |
PhosphoSitePlusi | Q8C0C7 |
SwissPalmi | Q8C0C7 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000003808, Expressed in spermatocyte and 248 other tissues |
ExpressionAtlasi | Q8C0C7, baseline and differential |
Genevisiblei | Q8C0C7, MM |
Interactioni
Subunit structurei
Heterotetramer; dimer of two heterodimers formed by FARSA and FARSB.
By similarityProtein-protein interaction databases
BioGRIDi | 211576, 10 interactors |
IntActi | Q8C0C7, 7 interactors |
MINTi | Q8C0C7 |
STRINGi | 10090.ENSMUSP00000003906 |
Miscellaneous databases
RNActi | Q8C0C7, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 372 – 374 | L-phenylalanine bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.Curated
Phylogenomic databases
eggNOGi | KOG2784, Eukaryota |
GeneTreei | ENSGT00390000006387 |
InParanoidi | Q8C0C7 |
OMAi | QIEGWVM |
OrthoDBi | 733355at2759 |
PhylomeDBi | Q8C0C7 |
TreeFami | TF300647 |
Family and domain databases
InterProi | View protein in InterPro IPR006195, aa-tRNA-synth_II IPR004529, Phe-tRNA-synth_IIc_asu IPR002319, Phenylalanyl-tRNA_Synthase IPR040724, PheRS_DBD1 IPR040586, PheRS_DBD2 IPR040725, PheRS_DBD3 IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF18552, PheRS_DBD1, 1 hit PF18554, PheRS_DBD2, 1 hit PF18553, PheRS_DBD3, 1 hit PF01409, tRNA-synt_2d, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
TIGRFAMsi | TIGR00468, pheS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q8C0C7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADNPVLELL LRRLEVADGG LDSAELATQL GVEHQAVVGA VKSLQALGEV
60 70 80 90 100
IEAELRSTKC WELTTEGEEI AREGSHEARV FRSIPLEGLV QSELMHLPSG
110 120 130 140 150
KVGFSKAMSN KWIRVDKSAA DGPRVFRVVD SIEDEVQKRL QLVQAGQAEK
160 170 180 190 200
LAEKERNELR KRKLLTEVIL KTYWVSKGKA FSTSVSKQEA ELSPEMISSG
210 220 230 240 250
SWRDRPFKPY NFSARGVLPD SGHLHPLLKV RSQFRQIFLE MGFTEMPTDN
260 270 280 290 300
FIESSFWNFD ALFQPQQHPA RDQHDTFFLR DPAEALQLPM GYVQRVKRTH
310 320 330 340 350
SQGGYGSQGY KYTWKLEEAR KNLLRTHTTA ASARALYQLA QKKPFTPAKY
360 370 380 390 400
FSIDRVFRNE TLDATHLAEF HQIEGVIADH GLTLGHLMGV LREFFTKLGI
410 420 430 440 450
TQLRFKPAYN PYTEPSMEVF SYHQGLKKWV EVGNSGVFRP EMLLPMGLPE
460 470 480 490 500
NVSVIAWGLS LERPTMIKYG INNIRELVGH KVNLQMVYDS PVCRLDIEPR
SSKTQEAA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PWY9 | E9PWY9_MOUSE | Phenylalanine--tRNA ligase | Farsa | 507 | Annotation score: | ||
D6RIJ2 | D6RIJ2_MOUSE | Phenylalanine--tRNA ligase alpha su... | Farsa | 105 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 291 | G → D in AAH06862 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 291 | G → D in AAH13533 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 343 | Missing in AAH13533 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 455 | I → T in AAH06862 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 455 | I → V in BAE32726 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK031697 mRNA Translation: BAC27519.1 AK154629 mRNA Translation: BAE32726.1 BC006862 mRNA Translation: AAH06862.1 BC013533 mRNA Translation: AAH13533.1 |
CCDSi | CCDS22480.2 |
RefSeqi | NP_079924.2, NM_025648.3 |
Genome annotation databases
Ensembli | ENSMUST00000003906; ENSMUSP00000003906; ENSMUSG00000003808 |
GeneIDi | 66590 |
KEGGi | mmu:66590 |
UCSCi | uc009mnt.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK031697 mRNA Translation: BAC27519.1 AK154629 mRNA Translation: BAE32726.1 BC006862 mRNA Translation: AAH06862.1 BC013533 mRNA Translation: AAH13533.1 |
CCDSi | CCDS22480.2 |
RefSeqi | NP_079924.2, NM_025648.3 |
3D structure databases
SMRi | Q8C0C7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 211576, 10 interactors |
IntActi | Q8C0C7, 7 interactors |
MINTi | Q8C0C7 |
STRINGi | 10090.ENSMUSP00000003906 |
PTM databases
iPTMneti | Q8C0C7 |
PhosphoSitePlusi | Q8C0C7 |
SwissPalmi | Q8C0C7 |
Proteomic databases
EPDi | Q8C0C7 |
jPOSTi | Q8C0C7 |
MaxQBi | Q8C0C7 |
PaxDbi | Q8C0C7 |
PeptideAtlasi | Q8C0C7 |
PRIDEi | Q8C0C7 |
Protocols and materials databases
Antibodypediai | 1072, 131 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000003906; ENSMUSP00000003906; ENSMUSG00000003808 |
GeneIDi | 66590 |
KEGGi | mmu:66590 |
UCSCi | uc009mnt.2, mouse |
Organism-specific databases
CTDi | 2193 |
MGIi | MGI:1913840, Farsa |
Phylogenomic databases
eggNOGi | KOG2784, Eukaryota |
GeneTreei | ENSGT00390000006387 |
InParanoidi | Q8C0C7 |
OMAi | QIEGWVM |
OrthoDBi | 733355at2759 |
PhylomeDBi | Q8C0C7 |
TreeFami | TF300647 |
Miscellaneous databases
BioGRID-ORCSi | 66590, 8 hits in 18 CRISPR screens |
ChiTaRSi | Farsa, mouse |
PROi | PR:Q8C0C7 |
RNActi | Q8C0C7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000003808, Expressed in spermatocyte and 248 other tissues |
ExpressionAtlasi | Q8C0C7, baseline and differential |
Genevisiblei | Q8C0C7, MM |
Family and domain databases
InterProi | View protein in InterPro IPR006195, aa-tRNA-synth_II IPR004529, Phe-tRNA-synth_IIc_asu IPR002319, Phenylalanyl-tRNA_Synthase IPR040724, PheRS_DBD1 IPR040586, PheRS_DBD2 IPR040725, PheRS_DBD3 IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF18552, PheRS_DBD1, 1 hit PF18554, PheRS_DBD2, 1 hit PF18553, PheRS_DBD3, 1 hit PF01409, tRNA-synt_2d, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
TIGRFAMsi | TIGR00468, pheS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYFA_MOUSE | |
Accessioni | Q8C0C7Primary (citable) accession number: Q8C0C7 Secondary accession number(s): Q3U3Q9, Q91WR4, Q922S1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 2007 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries