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Protein

Zinc fingers and homeoboxes protein 2

Gene

Zhx2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Represses the promoter activity of the CDC25C gene stimulated by NFYA (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri78 – 101C2H2-type 1PROSITE-ProRule annotationCuratedAdd BLAST24
Zinc fingeri110 – 133C2H2-type 2PROSITE-ProRule annotationCuratedAdd BLAST24
DNA bindingi263 – 324Homeobox 1PROSITE-ProRule annotationCuratedAdd BLAST62
DNA bindingi439 – 501Homeobox 2PROSITE-ProRule annotationCuratedAdd BLAST63
DNA bindingi530 – 591Homeobox 3PROSITE-ProRule annotationCuratedAdd BLAST62
DNA bindingi628 – 690Homeobox 4PROSITE-ProRule annotationCuratedAdd BLAST63

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc fingers and homeoboxes protein 2
Alternative name(s):
Alpha-fetoprotein regulator 1
Short name:
AFP regulator 1
Regulator of AFP
Zinc finger and homeodomain protein 2
Gene namesi
Name:Zhx2
Synonyms:Afr1, Raf
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2683087 Zhx2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000493921 – 836Zinc fingers and homeoboxes protein 2Add BLAST836

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki64Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki455Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei824PhosphoserineCombined sources1
Modified residuei826PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8C0C0
MaxQBiQ8C0C0
PaxDbiQ8C0C0
PRIDEiQ8C0C0

PTM databases

iPTMnetiQ8C0C0
PhosphoSitePlusiQ8C0C0

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000071757 Expressed in 202 organ(s), highest expression level in quadriceps femoris
CleanExiMM_ZHX2
GenevisibleiQ8C0C0 MM

Interactioni

Subunit structurei

Forms a homodimer (via homeobox domain). Forms a heterodimer with ZHX1 (via HD1 domain). Forms a heterodimer with ZHX3. Heterodimerization with ZHX1 is not necessary for repressor activity. Interacts (via homeobox domain) with NFYA (via N-terminus) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Dock1Q8BUR43EBI-646042,EBI-646023

GO - Molecular functioni

Protein-protein interaction databases

BioGridi239920, 2 interactors
IntActiQ8C0C0, 1 interactor
STRINGi10090.ENSMUSP00000094164

Structurei

3D structure databases

ProteinModelPortaliQ8C0C0
SMRiQ8C0C0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni195 – 358Required for homodimerizationBy similarityAdd BLAST164
Regioni263 – 497Required for interaction with NFYABy similarityAdd BLAST235
Regioni263 – 446Required for repressor activityBy similarityAdd BLAST184
Regioni317 – 446Required for nuclear localizationBy similarityAdd BLAST130

Sequence similaritiesi

Belongs to the ZHX family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri78 – 101C2H2-type 1PROSITE-ProRule annotationCuratedAdd BLAST24
Zinc fingeri110 – 133C2H2-type 2PROSITE-ProRule annotationCuratedAdd BLAST24

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IHET Eukaryota
ENOG410Z8A2 LUCA
GeneTreeiENSGT00530000063149
HOGENOMiHOG000013231
HOVERGENiHBG007920
InParanoidiQ8C0C0
OMAiLKHGISW
OrthoDBiEOG091G02WT
PhylomeDBiQ8C0C0
TreeFamiTF333363

Family and domain databases

CDDicd00086 homeodomain, 4 hits
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR001356 Homeobox_dom
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00046 Homeobox, 3 hits
SMARTiView protein in SMART
SM00389 HOX, 4 hits
SM00355 ZnF_C2H2, 2 hits
SUPFAMiSSF46689 SSF46689, 4 hits
SSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS50071 HOMEOBOX_2, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8C0C0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASKRKSTTP CMVRTSQVLE QDMLEEADRA KDKGAGMPQS DVTKDSWAAE
60 70 80 90 100
PEHSSKETEV VEVKSMGENL SKKLQGGYEC KYCPYSTQNL NEFTEHVDMQ
110 120 130 140 150
HPNVILNPLY VCAECNFTTK KYDSLSDHNS KFHPGETNFK LKLIKRNNQT
160 170 180 190 200
VLEQSIEATN HVVPITASGP GSSDNDPGVS VGKTPMTKTG KLKADAKKVP
210 220 230 240 250
KKPDEAAPEN HMEGTARLVT DTAEILARLG SVELLQDSLG HVMPSVQLPP
260 270 280 290 300
NINLVPKVPV PLNTTKYNSA LDTNATMINS FNKFPYPTQA ELSWLTAASK
310 320 330 340 350
HPEEHIRIWF ATQRLKHGIS WSPEEVEEAR KKMFNGTIQS VPPTITVLPA
360 370 380 390 400
QLTPTKVSQP ILQTALPCQI LGQPSLVLTQ VTSGSTTVSC SPITLAVAGV
410 420 430 440 450
TNHGQKRPLV TPQAAPEPKR PHIAQVPEPP PKVANTPLTP ASDRKKTKLQ
460 470 480 490 500
IAHLKASFLQ SQFPDDAEVY RLIEVTGLAR SEIKKWFSDH RYRCQRGIVH
510 520 530 540 550
ITSESLAKDQ MAITGTRHGR TYHVYPDFAP QKFKEKSQGQ LKTLEDSFLK
560 570 580 590 600
SSFPTQAEVE RLRVETKLSR REIDSWFSER RKLRDSMEQA VLDSMGSGKK
610 620 630 640 650
GSDAVAPNGA LSRLDQLSGA QLAGSLPSPS SAIVQNQEQV HLLRSTFART
660 670 680 690 700
QWPTPQEYDQ LAAKTGLVRT EIVRWFKENR CLLKTGTLSW LEQYQRHHMS
710 720 730 740 750
DDRGRDAVSR KVAKQVAESP KNGSEAAHQY AKDPKALSEE DSEKLVPRMK
760 770 780 790 800
VGGDPTKDCL AGKPSEATSD RSEGSRDGQG SEENEESGIV DFVEVTVGEE
810 820 830
DAISEKWGSW SRRVAEGTVE RADSDSDSTP AEAGQA
Length:836
Mass (Da):92,259
Last modified:March 1, 2003 - v1
Checksum:iC28AF20925D62054
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70L → P in BAC87710 (PubMed:14659886).Curated1
Sequence conflicti70L → P in BAC87712 (PubMed:14659886).Curated1
Sequence conflicti699M → L in BAC87710 (PubMed:14659886).Curated1
Sequence conflicti699M → L in BAC87712 (PubMed:14659886).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB099526 mRNA Translation: BAC87710.1
AB099704 Genomic DNA Translation: BAC87712.1
AK031782 mRNA Translation: BAC27546.1
BC059178 mRNA Translation: AAH59178.1
CCDSiCCDS27483.1
RefSeqiNP_955520.1, NM_199449.2
XP_011243990.1, XM_011245688.2
XP_011243991.1, XM_011245689.2
UniGeneiMm.36557

Genome annotation databases

EnsembliENSMUST00000096430; ENSMUSP00000094164; ENSMUSG00000071757
GeneIDi387609
KEGGimmu:387609
UCSCiuc007vsp.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB099526 mRNA Translation: BAC87710.1
AB099704 Genomic DNA Translation: BAC87712.1
AK031782 mRNA Translation: BAC27546.1
BC059178 mRNA Translation: AAH59178.1
CCDSiCCDS27483.1
RefSeqiNP_955520.1, NM_199449.2
XP_011243990.1, XM_011245688.2
XP_011243991.1, XM_011245689.2
UniGeneiMm.36557

3D structure databases

ProteinModelPortaliQ8C0C0
SMRiQ8C0C0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi239920, 2 interactors
IntActiQ8C0C0, 1 interactor
STRINGi10090.ENSMUSP00000094164

PTM databases

iPTMnetiQ8C0C0
PhosphoSitePlusiQ8C0C0

Proteomic databases

EPDiQ8C0C0
MaxQBiQ8C0C0
PaxDbiQ8C0C0
PRIDEiQ8C0C0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000096430; ENSMUSP00000094164; ENSMUSG00000071757
GeneIDi387609
KEGGimmu:387609
UCSCiuc007vsp.1 mouse

Organism-specific databases

CTDi22882
MGIiMGI:2683087 Zhx2

Phylogenomic databases

eggNOGiENOG410IHET Eukaryota
ENOG410Z8A2 LUCA
GeneTreeiENSGT00530000063149
HOGENOMiHOG000013231
HOVERGENiHBG007920
InParanoidiQ8C0C0
OMAiLKHGISW
OrthoDBiEOG091G02WT
PhylomeDBiQ8C0C0
TreeFamiTF333363

Miscellaneous databases

PROiPR:Q8C0C0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000071757 Expressed in 202 organ(s), highest expression level in quadriceps femoris
CleanExiMM_ZHX2
GenevisibleiQ8C0C0 MM

Family and domain databases

CDDicd00086 homeodomain, 4 hits
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR001356 Homeobox_dom
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00046 Homeobox, 3 hits
SMARTiView protein in SMART
SM00389 HOX, 4 hits
SM00355 ZnF_C2H2, 2 hits
SUPFAMiSSF46689 SSF46689, 4 hits
SSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS50071 HOMEOBOX_2, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiZHX2_MOUSE
AccessioniPrimary (citable) accession number: Q8C0C0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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