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Protein

Anaphase-promoting complex subunit 2

Gene

Anapc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Together with the RING-H2 protein ANAPC11, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation.By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Differentiation, Mitosis, Neurogenesis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-MMU-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-MMU-176408 Regulation of APC/C activators between G1/S and early anaphase
R-MMU-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-MMU-176412 Phosphorylation of the APC/C
R-MMU-179409 APC-Cdc20 mediated degradation of Nek2A
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit 2
Short name:
APC2
Alternative name(s):
Cyclosome subunit 2
Gene namesi
Name:Anapc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2139135 Anapc2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198121 – 837Anaphase-promoting complex subunit 2Add BLAST837

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei233PhosphoserineBy similarity1
Modified residuei329PhosphoserineBy similarity1
Modified residuei485PhosphoserineBy similarity1
Modified residuei549PhosphoserineCombined sources1
Modified residuei712PhosphoserineCombined sources1
Modified residuei825PhosphotyrosineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BZQ7
MaxQBiQ8BZQ7
PaxDbiQ8BZQ7
PRIDEiQ8BZQ7

PTM databases

iPTMnetiQ8BZQ7
PhosphoSitePlusiQ8BZQ7

Expressioni

Gene expression databases

BgeeiENSMUSG00000026965 Expressed in 297 organ(s), highest expression level in neocortex
CleanExiMM_ANAPC2
ExpressionAtlasiQ8BZQ7 baseline and differential
GenevisibleiQ8BZQ7 MM

Interactioni

Subunit structurei

The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5 (By similarity). In the context of the APC/C complex, directly interacts with UBE2C and UBE2S (By similarity). Interacts (via cullin domain) with ANAPC11 and with UBCH10 (By similarity). Interacts with NEUROD2 (PubMed:19900895).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi221196, 39 interactors
CORUMiQ8BZQ7
IntActiQ8BZQ7, 36 interactors
STRINGi10090.ENSMUSP00000028341

Structurei

3D structure databases

ProteinModelPortaliQ8BZQ7
SMRiQ8BZQ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2165 Eukaryota
ENOG410XRBY LUCA
GeneTreeiENSGT00390000016127
HOVERGENiHBG045327
InParanoidiQ8BZQ7
KOiK03349
OMAiSMVVDIY
OrthoDBiEOG091G07N8
TreeFamiTF105442

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR014786 APC_su2_C
IPR016158 Cullin_homology
IPR036317 Cullin_homology_sf
IPR001373 Cullin_N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF08672 ANAPC2, 1 hit
PF00888 Cullin, 1 hit
SMARTiView protein in SMART
SM01013 APC2, 1 hit
SM00182 CULLIN, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF75632 SSF75632, 1 hit
PROSITEiView protein in PROSITE
PS50069 CULLIN_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8BZQ7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEAEGVAVAA AAAAAAAAAT IIASDDCDSR PGQELLVAWN TVSTGLVPPA
60 70 80 90 100
ALGLASSRTS GAVPPKEEEL RAAVEVLRGH GLHSVLEEWF VEVLQNDLQG
110 120 130 140 150
NIATEFWNAI ALRENSVDEP QCLGLLLDAF GLLESRLDPY LHSLELLEKW
160 170 180 190 200
TRLGLLMGAG AQGLREKVHT MLRGVLFFST PRTFQEMVQR LYGRFLRVYM
210 220 230 240 250
QSKRKGEGGT DPELEGELDS RYARRRYYRL LQSPLCAGCG SDKQQCWCRQ
260 270 280 290 300
ALEQFNQLSQ VLHRLSLLER VCAEAVTTTL HQVTRERMED RCRGEYERSF
310 320 330 340 350
LREFHKWIER VVGWLGKVFL QDNPTRPTSP EAGNTLRRWR CHVQRFFYRI
360 370 380 390 400
YATLRIEELF SIIRDFPDSR PAIEDLKYCL ERTDQRQQLL VSLKVALETR
410 420 430 440 450
LLHPGVNTCD IITLYISAIK ALRVLDPSMV ILEVACEPIR RYLRTREDTV
460 470 480 490 500
RQIVAGLTGD SDGTGDLAVE LSKTDPACLE TGQDSEDDSG EPEDWVPDPV
510 520 530 540 550
DADPVKSSSK RRSSDIISLL VSIYGSKDLF INEYRSLLAD RLLHQFSFSP
560 570 580 590 600
EREIRNVELL KLRFGEAPMH FCEVMLKDMA DSRRINANIR EEDEKRPVEE
610 620 630 640 650
QPPFGVYAVI LSSEFWPPFK DEKLEVPEDI RAALDVYCKK YEKLKAMRTL
660 670 680 690 700
SWKHTLGLVT MDVELADRTL SVAVTPVQAV VLLYFQNQAS WTLEELSKVV
710 720 730 740 750
KMPVALLRRR MSVWLQQGVL REEPPGTFSV IEEERPQDRD NMVLIDSDDE
760 770 780 790 800
SDSGMASQAD QKEEELLLFW AYIQAMLTNL ESLSLERIYS MLRMFVMTGP
810 820 830
ALAEIDLQEL QGYLQKKVRD QQLIYSAGVY RLPKNSN
Length:837
Mass (Da):95,307
Last modified:July 27, 2011 - v2
Checksum:i02E62B928C0E20B3
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2AI11A2AI11_MOUSE
Anaphase-promoting complex subunit ...
Anapc2
185Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti680V → L in BAC28472 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033784 mRNA Translation: BAC28472.1
AK170676 mRNA Translation: BAE41951.1
AL732309 Genomic DNA No translation available.
BC027351 mRNA Translation: AAH27351.1
BC137583 mRNA Translation: AAI37584.1
CCDSiCCDS15761.1
RefSeqiNP_780509.2, NM_175300.4
UniGeneiMm.291624

Genome annotation databases

EnsembliENSMUST00000028341; ENSMUSP00000028341; ENSMUSG00000026965
GeneIDi99152
KEGGimmu:99152
UCSCiuc008ird.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033784 mRNA Translation: BAC28472.1
AK170676 mRNA Translation: BAE41951.1
AL732309 Genomic DNA No translation available.
BC027351 mRNA Translation: AAH27351.1
BC137583 mRNA Translation: AAI37584.1
CCDSiCCDS15761.1
RefSeqiNP_780509.2, NM_175300.4
UniGeneiMm.291624

3D structure databases

ProteinModelPortaliQ8BZQ7
SMRiQ8BZQ7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221196, 39 interactors
CORUMiQ8BZQ7
IntActiQ8BZQ7, 36 interactors
STRINGi10090.ENSMUSP00000028341

PTM databases

iPTMnetiQ8BZQ7
PhosphoSitePlusiQ8BZQ7

Proteomic databases

EPDiQ8BZQ7
MaxQBiQ8BZQ7
PaxDbiQ8BZQ7
PRIDEiQ8BZQ7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028341; ENSMUSP00000028341; ENSMUSG00000026965
GeneIDi99152
KEGGimmu:99152
UCSCiuc008ird.2 mouse

Organism-specific databases

CTDi29882
MGIiMGI:2139135 Anapc2

Phylogenomic databases

eggNOGiKOG2165 Eukaryota
ENOG410XRBY LUCA
GeneTreeiENSGT00390000016127
HOVERGENiHBG045327
InParanoidiQ8BZQ7
KOiK03349
OMAiSMVVDIY
OrthoDBiEOG091G07N8
TreeFamiTF105442

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-MMU-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-MMU-176408 Regulation of APC/C activators between G1/S and early anaphase
R-MMU-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-MMU-176412 Phosphorylation of the APC/C
R-MMU-179409 APC-Cdc20 mediated degradation of Nek2A
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiAnapc2 mouse
PROiPR:Q8BZQ7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026965 Expressed in 297 organ(s), highest expression level in neocortex
CleanExiMM_ANAPC2
ExpressionAtlasiQ8BZQ7 baseline and differential
GenevisibleiQ8BZQ7 MM

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR014786 APC_su2_C
IPR016158 Cullin_homology
IPR036317 Cullin_homology_sf
IPR001373 Cullin_N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF08672 ANAPC2, 1 hit
PF00888 Cullin, 1 hit
SMARTiView protein in SMART
SM01013 APC2, 1 hit
SM00182 CULLIN, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF75632 SSF75632, 1 hit
PROSITEiView protein in PROSITE
PS50069 CULLIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiANC2_MOUSE
AccessioniPrimary (citable) accession number: Q8BZQ7
Secondary accession number(s): Q3TCK5, Q8R2Q1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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