UniProtKB - Q8BZ89 (ZN322_MOUSE)

BLAST

>sp|Q8BZ89|ZN322_MOUSE Zinc finger protein 322 OS=Mus musculus OX=10090 GN=Znf322 PE=1 SV=1
MENHLGENHRRCTLQKRNVTRELKKGKHTMYALKRVKKIYIRVHEITQIDNQTYQCLERE
QNFCENLARMCERTYTEEKPYRCDMCEKTFIQSSDLISHQRIHNYEKPYKCSKCEKSFWH
HLALSGHQRTHAGKKFYTCDICGKNFGQSSDLLVHQRSHTGEKPYLCNECDKCFSRSTNL
IRHRRTHTGEKPFKCLECEKAFSGKSDLISHQRTHTGERPYKCNKCEKSYRHRSAFIVHK
RVHTGEKPYKCGACEKCFGQKSDLIVHQRVHTGEKPYKCLECMRSFTRSANLIRHQATHT
HTFKCLEYEKSFNCSSDFIVHQRIHMEEKPHQWSMCESDFLLGMDFVAQQKMRAQTEELH
YKYSVCDKTFHHSSALLQHQTVHIDDEYICNMSEKGLDLSSHASETSRVS

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History
Entry version 132 (07 Nov 2018)
Sequence version 1 (01 Mar 2003)
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Protein

Zinc finger protein 322

Gene

Znf322

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Transcriptional activator. Important for maintenance of pluripotency in embryonic stem cells. Binds directly to the POU5F1 distal enhancer and the NANOG proximal promoter, and enhances expression of both genes. Can also bind to numerous other gene promoters and regulates expression of many other pluripotency factors, either directly or indirectly. Promotes inhibition of MAPK signaling during embryonic stem cell differentiation.1 Publication

Miscellaneous

Significantly enhances POU5F1/OCT4-SOX2-KLF4-MYC (OSKM) mediated reprogramming of mouse embryonic fibroblasts into induced pluripotent stem cells, and can also substitute for SOX2 in this process.1 Publication

Regions

Feature key	Position(s)	DescriptionActions	Length
Zinc fingerⁱ	81 – 103	C2H2-type 1PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	109 – 131	C2H2-type 2PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	137 – 159	C2H2-type 3PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	165 – 187	C2H2-type 4PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	193 – 215	C2H2-type 5PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	221 – 243	C2H2-type 6PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	249 – 271	C2H2-type 7PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	277 – 299	C2H2-type 8PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	303 – 325	C2H2-type 9; degeneratePROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	361 – 383	C2H2-type 10; degeneratePROSITE-ProRule annotationAdd BLAST	23

GO - Molecular functionⁱ

DNA-binding transcription factor activity
Source: UniProtKB
Inferred from direct assayⁱ
- 24550733
enhancer binding
Source: UniProtKB
Inferred from direct assayⁱ
- 24550733
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

positive regulation of stem cell population maintenance
Source: UniProtKB
Inferred from direct assayⁱ
- 24550733
regulation of transcription, DNA-templated
Source: UniProtKB
Inferred from direct assayⁱ
- 24550733

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding
Biological process	Transcription, Transcription regulation
Ligand	Metal-binding, Zinc

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Zinc finger protein 322 Alternative name(s): Zinc finger protein 322A
Gene namesⁱ	Name:Znf322 Synonyms:Zfp322a, Znf322a
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 13

Organism-specific databases

MGIⁱ	MGI:2442566 Zfp322a

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000047531	1 – 410	Zinc finger protein 322Add BLAST		410

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	400	PhosphoserineBy similarity		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	Q8BZ89
PRIDEⁱ	Q8BZ89

PTM databases

iPTMnetⁱ	Q8BZ89
PhosphoSitePlusⁱ	Q8BZ89

Expressionⁱ

Developmental stageⁱ

Expressed in the inner cell mass of the blastocyst during preimplantation stage embryonic development.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000046351 Expressed in 293 organ(s), highest expression level in retina
CleanExⁱ	MM_ZFP322A
ExpressionAtlasⁱ	Q8BZ89 baseline and differential
Genevisibleⁱ	Q8BZ89 MM

Interactionⁱ

Subunit structureⁱ

Interacts with POU5F1.1 Publication

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000061524

STRINGⁱ

10090.ENSMUSP00000061524

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8BZ89
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the krueppel C2H2-type zinc-finger protein family.Curated

Zinc finger

Feature key	Position(s)	DescriptionActions	Length
Zinc fingerⁱ	81 – 103	C2H2-type 1PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	109 – 131	C2H2-type 2PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	137 – 159	C2H2-type 3PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	165 – 187	C2H2-type 4PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	193 – 215	C2H2-type 5PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	221 – 243	C2H2-type 6PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	249 – 271	C2H2-type 7PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	277 – 299	C2H2-type 8PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	303 – 325	C2H2-type 9; degeneratePROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	361 – 383	C2H2-type 10; degeneratePROSITE-ProRule annotationAdd BLAST	23

Keywords - Domainⁱ

Repeat, Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG1721 Eukaryota COG5048 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00930000150882
HOGENOMⁱ	HOG000234617
HOVERGENⁱ	HBG018163
InParanoidⁱ	Q8BZ89
OMAⁱ	STCESGF
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G02KC
PhylomeDBⁱ	Q8BZ89
TreeFamⁱ	TF338126

Family and domain databases

InterProⁱ	View protein in InterPro IPR036236 Znf_C2H2_sf IPR013087 Znf_C2H2_type
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00096 zf-C2H2, 7 hits
SMARTⁱ	View protein in SMART SM00355 ZnF_C2H2, 10 hits
SUPFAMⁱ	SSF57667 SSF57667, 6 hits
PROSITEⁱ	View protein in PROSITE PS00028 ZINC_FINGER_C2H2_1, 8 hits PS50157 ZINC_FINGER_C2H2_2, 10 hits

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show all Align All

Q8BZ89-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MENHLGENHR RCTLQKRNVT RELKKGKHTM YALKRVKKIY IRVHEITQID 
        60         70         80         90        100
NQTYQCLERE QNFCENLARM CERTYTEEKP YRCDMCEKTF IQSSDLISHQ 
       110        120        130        140        150
RIHNYEKPYK CSKCEKSFWH HLALSGHQRT HAGKKFYTCD ICGKNFGQSS 
       160        170        180        190        200
DLLVHQRSHT GEKPYLCNEC DKCFSRSTNL IRHRRTHTGE KPFKCLECEK 
       210        220        230        240        250
AFSGKSDLIS HQRTHTGERP YKCNKCEKSY RHRSAFIVHK RVHTGEKPYK 
       260        270        280        290        300
CGACEKCFGQ KSDLIVHQRV HTGEKPYKCL ECMRSFTRSA NLIRHQATHT 
       310        320        330        340        350
HTFKCLEYEK SFNCSSDFIV HQRIHMEEKP HQWSMCESDF LLGMDFVAQQ 
       360        370        380        390        400
KMRAQTEELH YKYSVCDKTF HHSSALLQHQ TVHIDDEYIC NMSEKGLDLS 
       410
SHASETSRVS

Length:410

Mass (Da):48,492

Last modified:March 1, 2003 - v1

Checksum:ⁱ27E5668CC383FAA7

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

B7ZCB3	B7ZCB3_MOUSE	Zinc finger protein 322A Zinc finger protein 322A	Zfp322a	126	Annotation score:
B7ZCB1	B7ZCB1_MOUSE	Zinc finger protein 322A Zinc finger protein 322A	Zfp322a	179	Annotation score:
A0A1B0GX55	A0A1B0GX55_MOUSE	Zinc finger protein 322A Zinc finger protein 322A	Zfp322a	180	Annotation score:

Sequence cautionⁱ

The sequence AAH32268 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	1	M → V in AAH32268 (PubMed:15489334).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK036302 mRNA Translation: BAC29377.1 AK162608 mRNA Translation: BAE36986.1 AK170525 mRNA Translation: BAE41858.1 AL669819 Genomic DNA No translation available. BC017143 mRNA Translation: AAH17143.1 BC032268 mRNA Translation: AAH32268.1 Different initiation. BC043711 mRNA Translation: AAH43711.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS26337.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001104577.1, NM_001111107.2 NP_001273084.1, NM_001286155.1 NP_001273085.1, NM_001286156.1 NP_766174.1, NM_172586.4
UniGeneⁱ	Mm.286454

Genome annotation databases

Ensemblⁱ	ENSMUST00000050101; ENSMUSP00000061524; ENSMUSG00000046351
GeneIDⁱ	218100
KEGGⁱ	mmu:218100
UCSC genome browser More...UCSCⁱ	uc007ptn.3 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q8BZ89	Zinc finger protein 322A (Fragment)		MOUSE		179	UniRef100_Q8BZ89
Zinc finger protein 322A (Fragment)		MOUSE		180
Zinc finger protein 322A (Fragment)		MOUSE		126

Protein	Similar proteins		Species	Score	Length	Source
Q8BZ89	Zinc finger protein 322A (Fragment)		MOUSE		179	UniRef90_Q8BZ89
RCG45242, isoform CRA_a		RAT		399
zinc finger protein 322		MESAU		335
UPI000A30AC07		MUSPA		410
UPI000A315FDB		MUSCR		399
+4

Protein	Similar proteins		Species	Score	Length	Source
Q8BZ89	Zinc finger protein 322		HUMAN		402	UniRef50_Q8BZ89
Zinc finger protein 322		MACFA		402
Zinc finger protein 322A (Fragment)		MOUSE		179
RCG45242, isoform CRA_a		RAT		399
zinc finger protein 322		MESAU		335
+127

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK036302 mRNA Translation: BAC29377.1 AK162608 mRNA Translation: BAE36986.1 AK170525 mRNA Translation: BAE41858.1 AL669819 Genomic DNA No translation available. BC017143 mRNA Translation: AAH17143.1 BC032268 mRNA Translation: AAH32268.1 Different initiation. BC043711 mRNA Translation: AAH43711.1
CCDSⁱ	CCDS26337.1
RefSeqⁱ	NP_001104577.1, NM_001111107.2 NP_001273084.1, NM_001286155.1 NP_001273085.1, NM_001286156.1 NP_766174.1, NM_172586.4
UniGeneⁱ	Mm.286454

3D structure databases

ProteinModelPortalⁱ	Q8BZ89
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000061524

STRINGⁱ

10090.ENSMUSP00000061524

PTM databases

iPTMnetⁱ	Q8BZ89
PhosphoSitePlusⁱ	Q8BZ89

Proteomic databases

PaxDbⁱ	Q8BZ89
PRIDEⁱ	Q8BZ89

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000050101; ENSMUSP00000061524; ENSMUSG00000046351
GeneIDⁱ	218100
KEGGⁱ	mmu:218100
UCSCⁱ	uc007ptn.3 mouse

Organism-specific databases

CTDⁱ	218100
MGIⁱ	MGI:2442566 Zfp322a

Phylogenomic databases

eggNOGⁱ	KOG1721 Eukaryota COG5048 LUCA
GeneTreeⁱ	ENSGT00930000150882
HOGENOMⁱ	HOG000234617
HOVERGENⁱ	HBG018163
InParanoidⁱ	Q8BZ89
OMAⁱ	STCESGF
OrthoDBⁱ	EOG091G02KC
PhylomeDBⁱ	Q8BZ89
TreeFamⁱ	TF338126

Miscellaneous databases

ChiTaRSⁱ	Zfp322a mouse
Protein Ontology More...PROⁱ	PR:Q8BZ89
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000046351 Expressed in 293 organ(s), highest expression level in retina
CleanExⁱ	MM_ZFP322A
ExpressionAtlasⁱ	Q8BZ89 baseline and differential
Genevisibleⁱ	Q8BZ89 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR036236 Znf_C2H2_sf IPR013087 Znf_C2H2_type
Pfamⁱ	View protein in Pfam PF00096 zf-C2H2, 7 hits
SMARTⁱ	View protein in SMART SM00355 ZnF_C2H2, 10 hits
SUPFAMⁱ	SSF57667 SSF57667, 6 hits
PROSITEⁱ	View protein in PROSITE PS00028 ZINC_FINGER_C2H2_1, 8 hits PS50157 ZINC_FINGER_C2H2_2, 10 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ZN322_MOUSE
Accessionⁱ	Q8BZ89Primary (citable) accession number: Q8BZ89 Secondary accession number(s): Q8K0A0, Q91W42
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
	Last sequence update:	March 1, 2003
	Last modified:	November 7, 2018
	This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8BZ89 (ZN322_MOUSE)

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Zinc finger protein 322

Znf322

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cytoskeleton

Cytosol

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ