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Protein

Zinc finger protein 322

Gene

Znf322

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator. Important for maintenance of pluripotency in embryonic stem cells. Binds directly to the POU5F1 distal enhancer and the NANOG proximal promoter, and enhances expression of both genes. Can also bind to numerous other gene promoters and regulates expression of many other pluripotency factors, either directly or indirectly. Promotes inhibition of MAPK signaling during embryonic stem cell differentiation.1 Publication

Miscellaneous

Significantly enhances POU5F1/OCT4-SOX2-KLF4-MYC (OSKM) mediated reprogramming of mouse embryonic fibroblasts into induced pluripotent stem cells, and can also substitute for SOX2 in this process.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri81 – 103C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri109 – 131C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri137 – 159C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri165 – 187C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri193 – 215C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri221 – 243C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri249 – 271C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri277 – 299C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri303 – 325C2H2-type 9; degeneratePROSITE-ProRule annotationAdd BLAST23
Zinc fingeri361 – 383C2H2-type 10; degeneratePROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

  • DNA-binding transcription factor activity Source: UniProtKB
  • enhancer binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 322
Alternative name(s):
Zinc finger protein 322A
Gene namesi
Name:Znf322
Synonyms:Zfp322a, Znf322a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2442566 Zfp322a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000475311 – 410Zinc finger protein 322Add BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei400PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8BZ89
PRIDEiQ8BZ89

PTM databases

iPTMnetiQ8BZ89
PhosphoSitePlusiQ8BZ89

Expressioni

Developmental stagei

Expressed in the inner cell mass of the blastocyst during preimplantation stage embryonic development.1 Publication

Gene expression databases

BgeeiENSMUSG00000046351 Expressed in 293 organ(s), highest expression level in retina
CleanExiMM_ZFP322A
ExpressionAtlasiQ8BZ89 baseline and differential
GenevisibleiQ8BZ89 MM

Interactioni

Subunit structurei

Interacts with POU5F1.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000061524

Structurei

3D structure databases

ProteinModelPortaliQ8BZ89
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri81 – 103C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri109 – 131C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri137 – 159C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri165 – 187C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri193 – 215C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri221 – 243C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri249 – 271C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri277 – 299C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri303 – 325C2H2-type 9; degeneratePROSITE-ProRule annotationAdd BLAST23
Zinc fingeri361 – 383C2H2-type 10; degeneratePROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00930000150882
HOGENOMiHOG000234617
HOVERGENiHBG018163
InParanoidiQ8BZ89
OMAiSTCESGF
OrthoDBiEOG091G02KC
PhylomeDBiQ8BZ89
TreeFamiTF338126

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 7 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 10 hits
SUPFAMiSSF57667 SSF57667, 6 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 8 hits
PS50157 ZINC_FINGER_C2H2_2, 10 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q8BZ89-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MENHLGENHR RCTLQKRNVT RELKKGKHTM YALKRVKKIY IRVHEITQID
60 70 80 90 100
NQTYQCLERE QNFCENLARM CERTYTEEKP YRCDMCEKTF IQSSDLISHQ
110 120 130 140 150
RIHNYEKPYK CSKCEKSFWH HLALSGHQRT HAGKKFYTCD ICGKNFGQSS
160 170 180 190 200
DLLVHQRSHT GEKPYLCNEC DKCFSRSTNL IRHRRTHTGE KPFKCLECEK
210 220 230 240 250
AFSGKSDLIS HQRTHTGERP YKCNKCEKSY RHRSAFIVHK RVHTGEKPYK
260 270 280 290 300
CGACEKCFGQ KSDLIVHQRV HTGEKPYKCL ECMRSFTRSA NLIRHQATHT
310 320 330 340 350
HTFKCLEYEK SFNCSSDFIV HQRIHMEEKP HQWSMCESDF LLGMDFVAQQ
360 370 380 390 400
KMRAQTEELH YKYSVCDKTF HHSSALLQHQ TVHIDDEYIC NMSEKGLDLS
410
SHASETSRVS
Length:410
Mass (Da):48,492
Last modified:March 1, 2003 - v1
Checksum:i27E5668CC383FAA7
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7ZCB3B7ZCB3_MOUSE
Zinc finger protein 322A
Zfp322a
126Annotation score:
B7ZCB1B7ZCB1_MOUSE
Zinc finger protein 322A
Zfp322a
179Annotation score:
A0A1B0GX55A0A1B0GX55_MOUSE
Zinc finger protein 322A
Zfp322a
180Annotation score:

Sequence cautioni

The sequence AAH32268 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1M → V in AAH32268 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036302 mRNA Translation: BAC29377.1
AK162608 mRNA Translation: BAE36986.1
AK170525 mRNA Translation: BAE41858.1
AL669819 Genomic DNA No translation available.
BC017143 mRNA Translation: AAH17143.1
BC032268 mRNA Translation: AAH32268.1 Different initiation.
BC043711 mRNA Translation: AAH43711.1
CCDSiCCDS26337.1
RefSeqiNP_001104577.1, NM_001111107.2
NP_001273084.1, NM_001286155.1
NP_001273085.1, NM_001286156.1
NP_766174.1, NM_172586.4
UniGeneiMm.286454

Genome annotation databases

EnsembliENSMUST00000050101; ENSMUSP00000061524; ENSMUSG00000046351
GeneIDi218100
KEGGimmu:218100
UCSCiuc007ptn.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036302 mRNA Translation: BAC29377.1
AK162608 mRNA Translation: BAE36986.1
AK170525 mRNA Translation: BAE41858.1
AL669819 Genomic DNA No translation available.
BC017143 mRNA Translation: AAH17143.1
BC032268 mRNA Translation: AAH32268.1 Different initiation.
BC043711 mRNA Translation: AAH43711.1
CCDSiCCDS26337.1
RefSeqiNP_001104577.1, NM_001111107.2
NP_001273084.1, NM_001286155.1
NP_001273085.1, NM_001286156.1
NP_766174.1, NM_172586.4
UniGeneiMm.286454

3D structure databases

ProteinModelPortaliQ8BZ89
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000061524

PTM databases

iPTMnetiQ8BZ89
PhosphoSitePlusiQ8BZ89

Proteomic databases

PaxDbiQ8BZ89
PRIDEiQ8BZ89

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050101; ENSMUSP00000061524; ENSMUSG00000046351
GeneIDi218100
KEGGimmu:218100
UCSCiuc007ptn.3 mouse

Organism-specific databases

CTDi218100
MGIiMGI:2442566 Zfp322a

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00930000150882
HOGENOMiHOG000234617
HOVERGENiHBG018163
InParanoidiQ8BZ89
OMAiSTCESGF
OrthoDBiEOG091G02KC
PhylomeDBiQ8BZ89
TreeFamiTF338126

Miscellaneous databases

ChiTaRSiZfp322a mouse
PROiPR:Q8BZ89
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000046351 Expressed in 293 organ(s), highest expression level in retina
CleanExiMM_ZFP322A
ExpressionAtlasiQ8BZ89 baseline and differential
GenevisibleiQ8BZ89 MM

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 7 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 10 hits
SUPFAMiSSF57667 SSF57667, 6 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 8 hits
PS50157 ZINC_FINGER_C2H2_2, 10 hits
ProtoNetiSearch...

Entry informationi

Entry nameiZN322_MOUSE
AccessioniPrimary (citable) accession number: Q8BZ89
Secondary accession number(s): Q8K0A0, Q91W42
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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