UniProtKB - Q8BZ89 (ZN322_MOUSE)
Zinc finger protein 322
Znf322
Functioni
Transcriptional activator. Important for maintenance of pluripotency in embryonic stem cells. Binds directly to the POU5F1 distal enhancer and the NANOG proximal promoter, and enhances expression of both genes. Can also bind to numerous other gene promoters and regulates expression of many other pluripotency factors, either directly or indirectly. Promotes inhibition of MAPK signaling during embryonic stem cell differentiation.
1 PublicationMiscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 81 – 103 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 109 – 131 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 137 – 159 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 165 – 187 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 193 – 215 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 221 – 243 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 249 – 271 | C2H2-type 7PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 277 – 299 | C2H2-type 8PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 303 – 325 | C2H2-type 9; degeneratePROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 361 – 383 | C2H2-type 10; degeneratePROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- positive regulation of stem cell population maintenance Source: UniProtKB
- regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein 322Alternative name(s): Zinc finger protein 322A |
Gene namesi | Name:Znf322 Synonyms:Zfp322a, Znf322a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2442566, Zfp322a |
VEuPathDBi | HostDB:ENSMUSG00000046351 |
Subcellular locationi
Cytoskeleton
- centrosome Source: MGI
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047531 | 1 – 410 | Zinc finger protein 322Add BLAST | 410 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 400 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8BZ89 |
PRIDEi | Q8BZ89 |
ProteomicsDBi | 275281 |
PTM databases
iPTMneti | Q8BZ89 |
PhosphoSitePlusi | Q8BZ89 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000046351, Expressed in forelimb bud and 315 other tissues |
Genevisiblei | Q8BZ89, MM |
Interactioni
Subunit structurei
Interacts with POU5F1.
1 PublicationProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000061524 |
Miscellaneous databases
RNActi | Q8BZ89, protein |
Family & Domainsi
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 81 – 103 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 109 – 131 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 137 – 159 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 165 – 187 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 193 – 215 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 221 – 243 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 249 – 271 | C2H2-type 7PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 277 – 299 | C2H2-type 8PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 303 – 325 | C2H2-type 9; degeneratePROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 361 – 383 | C2H2-type 10; degeneratePROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000162956 |
HOGENOMi | CLU_002678_44_10_1 |
InParanoidi | Q8BZ89 |
OMAi | CESDFLL |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q8BZ89 |
TreeFami | TF338126 |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 7 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 10 hits |
SUPFAMi | SSF57667, SSF57667, 6 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 8 hits PS50157, ZINC_FINGER_C2H2_2, 10 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MENHLGENHR RCTLQKRNVT RELKKGKHTM YALKRVKKIY IRVHEITQID
60 70 80 90 100
NQTYQCLERE QNFCENLARM CERTYTEEKP YRCDMCEKTF IQSSDLISHQ
110 120 130 140 150
RIHNYEKPYK CSKCEKSFWH HLALSGHQRT HAGKKFYTCD ICGKNFGQSS
160 170 180 190 200
DLLVHQRSHT GEKPYLCNEC DKCFSRSTNL IRHRRTHTGE KPFKCLECEK
210 220 230 240 250
AFSGKSDLIS HQRTHTGERP YKCNKCEKSY RHRSAFIVHK RVHTGEKPYK
260 270 280 290 300
CGACEKCFGQ KSDLIVHQRV HTGEKPYKCL ECMRSFTRSA NLIRHQATHT
310 320 330 340 350
HTFKCLEYEK SFNCSSDFIV HQRIHMEEKP HQWSMCESDF LLGMDFVAQQ
360 370 380 390 400
KMRAQTEELH YKYSVCDKTF HHSSALLQHQ TVHIDDEYIC NMSEKGLDLS
410
SHASETSRVS
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB7ZCB1 | B7ZCB1_MOUSE | Zinc finger protein 322A | Zfp322a | 179 | Annotation score: | ||
B7ZCB3 | B7ZCB3_MOUSE | Zinc finger protein 322A | Zfp322a | 126 | Annotation score: | ||
A0A1B0GX55 | A0A1B0GX55_MOUSE | Zinc finger protein 322A | Zfp322a | 180 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 | M → V in AAH32268 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK036302 mRNA Translation: BAC29377.1 AK162608 mRNA Translation: BAE36986.1 AK170525 mRNA Translation: BAE41858.1 AL669819 Genomic DNA No translation available. BC017143 mRNA Translation: AAH17143.1 BC032268 mRNA Translation: AAH32268.1 Different initiation. BC043711 mRNA Translation: AAH43711.1 |
CCDSi | CCDS26337.1 |
RefSeqi | NP_001104577.1, NM_001111107.2 NP_001273084.1, NM_001286155.1 NP_001273085.1, NM_001286156.1 NP_766174.1, NM_172586.4 |
Genome annotation databases
Ensembli | ENSMUST00000050101; ENSMUSP00000061524; ENSMUSG00000046351 |
GeneIDi | 218100 |
KEGGi | mmu:218100 |
UCSCi | uc007ptn.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK036302 mRNA Translation: BAC29377.1 AK162608 mRNA Translation: BAE36986.1 AK170525 mRNA Translation: BAE41858.1 AL669819 Genomic DNA No translation available. BC017143 mRNA Translation: AAH17143.1 BC032268 mRNA Translation: AAH32268.1 Different initiation. BC043711 mRNA Translation: AAH43711.1 |
CCDSi | CCDS26337.1 |
RefSeqi | NP_001104577.1, NM_001111107.2 NP_001273084.1, NM_001286155.1 NP_001273085.1, NM_001286156.1 NP_766174.1, NM_172586.4 |
3D structure databases
SMRi | Q8BZ89 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000061524 |
PTM databases
iPTMneti | Q8BZ89 |
PhosphoSitePlusi | Q8BZ89 |
Proteomic databases
PaxDbi | Q8BZ89 |
PRIDEi | Q8BZ89 |
ProteomicsDBi | 275281 |
Protocols and materials databases
Antibodypediai | 11103, 122 antibodies from 19 providers |
DNASUi | 218100 |
Genome annotation databases
Ensembli | ENSMUST00000050101; ENSMUSP00000061524; ENSMUSG00000046351 |
GeneIDi | 218100 |
KEGGi | mmu:218100 |
UCSCi | uc007ptn.3, mouse |
Organism-specific databases
CTDi | 218100 |
MGIi | MGI:2442566, Zfp322a |
VEuPathDBi | HostDB:ENSMUSG00000046351 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000162956 |
HOGENOMi | CLU_002678_44_10_1 |
InParanoidi | Q8BZ89 |
OMAi | CESDFLL |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q8BZ89 |
TreeFami | TF338126 |
Miscellaneous databases
BioGRID-ORCSi | 218100, 0 hits in 63 CRISPR screens |
ChiTaRSi | Zfp322a, mouse |
PROi | PR:Q8BZ89 |
RNActi | Q8BZ89, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000046351, Expressed in forelimb bud and 315 other tissues |
Genevisiblei | Q8BZ89, MM |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 7 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 10 hits |
SUPFAMi | SSF57667, SSF57667, 6 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 8 hits PS50157, ZINC_FINGER_C2H2_2, 10 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ZN322_MOUSE | |
Accessioni | Q8BZ89Primary (citable) accession number: Q8BZ89 Secondary accession number(s): Q8K0A0, Q91W42 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families