UniProtKB - Q8BYW9 (EOGT_MOUSE)
Protein
EGF domain-specific O-linked N-acetylglucosamine transferase
Gene
Eogt
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains.1 Publication
Catalytic activityi
- L-seryl-[protein] + UDP-N-acetyl-α-D-glucosamine = 3-O-(N-acetyl-β-D-glucosaminyl)-L-seryl-[protein] + H+ + UDP1 PublicationEC:2.4.1.2551 Publication
- L-threonyl-[protein] + UDP-N-acetyl-α-D-glucosamine = 3-O-(N-acetyl-β-D-glucosaminyl)-L-threonyl-[protein] + H+ + UDP1 PublicationEC:2.4.1.2551 Publication
GO - Molecular functioni
- protein N-acetylglucosaminyltransferase activity Source: UniProtKB
- protein O-GlcNAc transferase activity Source: UniProtKB-EC
- transferase activity, transferring glycosyl groups Source: GO_Central
GO - Biological processi
- protein O-GlcNAcylation via threonine Source: GO_Central
- protein O-linked glycosylation Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Protein family/group databases
CAZyi | GT61, Glycosyltransferase Family 61 |
Names & Taxonomyi
Protein namesi | Recommended name: EGF domain-specific O-linked N-acetylglucosamine transferase (EC:2.4.1.2551 Publication)Alternative name(s): Extracellular O-linked N-acetylglucosamine transferase |
Gene namesi | Name:Eogt Synonyms:Aer61, Eogt1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2141669, Eogt |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen PROSITE-ProRule annotation
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: GO_Central
Keywords - Cellular componenti
Endoplasmic reticulumPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000301972 | 20 – 527 | EGF domain-specific O-linked N-acetylglucosamine transferaseAdd BLAST | 508 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 354 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | Q8BYW9 |
MaxQBi | Q8BYW9 |
PaxDbi | Q8BYW9 |
PeptideAtlasi | Q8BYW9 |
PRIDEi | Q8BYW9 |
PTM databases
GlyConnecti | 2279, 2 N-Linked glycans (1 site) |
GlyGeni | Q8BYW9, 1 site |
PhosphoSitePlusi | Q8BYW9 |
Expressioni
Tissue specificityi
Widely expressed. Expressed in brain, heart, kidney, lung, skeletal muscles and thymus. Highest expression is observed in lung and the lowest in skeletal muscles.1 Publication
Developmental stagei
Expressed at embryonic day (E) 10.5 in the growing edge of the limb buds. At 11.5 dpc, enriched in the apical ectodermal ridge of the limbs. By 12.5 dpc, expression assumes a digit-condensation pattern in the 4 limbs.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000035245, Expressed in embryonic post-anal tail and 307 other tissues |
ExpressionAtlasi | Q8BYW9, baseline and differential |
Genevisiblei | Q8BYW9, MM |
Interactioni
Protein-protein interaction databases
IntActi | Q8BYW9, 1 interactor |
STRINGi | 10090.ENSMUSP00000061610 |
Miscellaneous databases
RNActi | Q8BYW9, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 295 – 297 | Required for optimal activityBy similarity | 3 | |
Motifi | 524 – 527 | Prevents secretion from ERPROSITE-ProRule annotation | 4 |
Sequence similaritiesi
Belongs to the glycosyltransferase 61 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4698, Eukaryota |
GeneTreei | ENSGT00940000156493 |
HOGENOMi | CLU_039300_0_0_1 |
InParanoidi | Q8BYW9 |
OMAi | DFGYIQE |
OrthoDBi | 567582at2759 |
PhylomeDBi | Q8BYW9 |
TreeFami | TF313716 |
Family and domain databases
InterProi | View protein in InterPro IPR007657, Glycosyltransferase_61 |
PANTHERi | PTHR20961, PTHR20961, 1 hit |
Pfami | View protein in Pfam PF04577, DUF563, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q8BYW9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLMLLVFGVL LHEVPLSGQD KAHSEADDAP GKALYDYSSL RLPAEHIPFF
60 70 80 90 100
LHNNRHVASV CREDSHCPYK KHLENLNYCW GYEKSCAPEF RFGSPVCSYV
110 120 130 140 150
DLGWTDTLES AQDMFWRQAD FGYARERLGE IRTICQPERA SDSSLVCSRY
160 170 180 190 200
LQYCRATGLY LDLRNIKRNH DRFKEDFLQG GEIGGYCKLD SHALVSEGQR
210 220 230 240 250
KSPLQSWFAE LQGYTQLNFR PIEDAKCDIV VEKPTYFMKL DAGINMYHHF
260 270 280 290 300
CDFLNLYLTQ HVNNSFSTDV YIVMWDTSTY GYGDLFSDTW KAFTDYDVIH
310 320 330 340 350
LKTYDSKKVC FKEAVFSLLP RMRYGLFYNT PLISGCQNTG LFRAFSQHVL
360 370 380 390 400
HRLNITQEGP KDGKVRVTIL ARSTEYRKIL NQDELVNALK TVSTFEVRVV
410 420 430 440 450
DYKYRELGFL DQLRITHNTD IFIGMHGAGL THLLFLPDWA AVFELYNCED
460 470 480 490 500
ERCYLDLARL RGIHYITWRK PSKVFPQDKG HHPTLGEHPK FTNYSFDVEE
510 520
FMYLVLQAAE HVLQHPQWPF KKKHDEL
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3Z459 | D3Z459_MOUSE | EGF domain-specific O-linked N-acet... | Eogt A130022J15Rik | 148 | Annotation score: | ||
D3YWW8 | D3YWW8_MOUSE | EGF domain-specific O-linked N-acet... | Eogt A130022J15Rik | 90 | Annotation score: | ||
D3Z5Q3 | D3Z5Q3_MOUSE | EGF domain-specific O-linked N-acet... | Eogt A130022J15Rik | 49 | Annotation score: | ||
D3Z052 | D3Z052_MOUSE | EGF domain-specific O-linked N-acet... | Eogt A130022J15Rik | 61 | Annotation score: | ||
A0A0N4SVY8 | A0A0N4SVY8_MOUSE | EGF domain-specific O-linked N-acet... | Eogt | 67 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK037507 mRNA Translation: BAC29821.1 BC048939 mRNA Translation: AAH48939.1 |
CCDSi | CCDS20383.1 |
RefSeqi | NP_780522.1, NM_175313.4 XP_006505321.1, XM_006505258.3 |
Genome annotation databases
Ensembli | ENSMUST00000054344; ENSMUSP00000061610; ENSMUSG00000035245 |
GeneIDi | 101351 |
KEGGi | mmu:101351 |
UCSCi | uc009dai.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK037507 mRNA Translation: BAC29821.1 BC048939 mRNA Translation: AAH48939.1 |
CCDSi | CCDS20383.1 |
RefSeqi | NP_780522.1, NM_175313.4 XP_006505321.1, XM_006505258.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q8BYW9, 1 interactor |
STRINGi | 10090.ENSMUSP00000061610 |
Protein family/group databases
CAZyi | GT61, Glycosyltransferase Family 61 |
PTM databases
GlyConnecti | 2279, 2 N-Linked glycans (1 site) |
GlyGeni | Q8BYW9, 1 site |
PhosphoSitePlusi | Q8BYW9 |
Proteomic databases
EPDi | Q8BYW9 |
MaxQBi | Q8BYW9 |
PaxDbi | Q8BYW9 |
PeptideAtlasi | Q8BYW9 |
PRIDEi | Q8BYW9 |
Protocols and materials databases
Antibodypediai | 15479, 47 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000054344; ENSMUSP00000061610; ENSMUSG00000035245 |
GeneIDi | 101351 |
KEGGi | mmu:101351 |
UCSCi | uc009dai.1, mouse |
Organism-specific databases
CTDi | 285203 |
MGIi | MGI:2141669, Eogt |
Phylogenomic databases
eggNOGi | KOG4698, Eukaryota |
GeneTreei | ENSGT00940000156493 |
HOGENOMi | CLU_039300_0_0_1 |
InParanoidi | Q8BYW9 |
OMAi | DFGYIQE |
OrthoDBi | 567582at2759 |
PhylomeDBi | Q8BYW9 |
TreeFami | TF313716 |
Miscellaneous databases
BioGRID-ORCSi | 101351, 0 hits in 17 CRISPR screens |
ChiTaRSi | Eogt, mouse |
PROi | PR:Q8BYW9 |
RNActi | Q8BYW9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000035245, Expressed in embryonic post-anal tail and 307 other tissues |
ExpressionAtlasi | Q8BYW9, baseline and differential |
Genevisiblei | Q8BYW9, MM |
Family and domain databases
InterProi | View protein in InterPro IPR007657, Glycosyltransferase_61 |
PANTHERi | PTHR20961, PTHR20961, 1 hit |
Pfami | View protein in Pfam PF04577, DUF563, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EOGT_MOUSE | |
Accessioni | Q8BYW9Primary (citable) accession number: Q8BYW9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families