UniProtKB - Q8BYK5 (PHAR3_MOUSE)
Protein
Phosphatase and actin regulator 3
Gene
Phactr3
Organism
Mus musculus (Mouse)
Status
Functioni
GO - Molecular functioni
- actin binding Source: MGI
- protein phosphatase 1 binding Source: InterPro
- protein phosphatase inhibitor activity Source: UniProtKB-KW
GO - Biological processi
- actin cytoskeleton organization Source: GO_Central
Keywordsi
Molecular function | Actin-binding, Protein phosphatase inhibitor |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatase and actin regulator 3Alternative name(s): Scaffold-associated PP1-inhibiting protein Short name: Scapinin |
Gene namesi | Name:Phactr3 Synonyms:Scapin1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1921439, Phactr3 |
Subcellular locationi
Nucleus
- Nucleus matrix By similarity
Note: Localized to the nuclear matrix-intermediate filament scaffold.By similarity
Nucleus
- nuclear matrix Source: UniProtKB-SubCell
- nucleoplasm Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000126639 | 1 – 558 | Phosphatase and actin regulator 3Add BLAST | 558 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 69 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 229 | PhosphoserineCombined sources | 1 | |
Modified residuei | 235 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8BYK5 |
PRIDEi | Q8BYK5 |
PTM databases
iPTMneti | Q8BYK5 |
PhosphoSitePlusi | Q8BYK5 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000027525, Expressed in lumbar subsegment of spinal cord and 150 other tissues |
ExpressionAtlasi | Q8BYK5, baseline and differential |
Genevisiblei | Q8BYK5, MM |
Interactioni
Subunit structurei
Binds PPP1CA and actin; thus inhibiting the protein phosphatase 1 (PP1) activity.
By similarityGO - Molecular functioni
- actin binding Source: MGI
- protein phosphatase 1 binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 216561, 2 interactors |
STRINGi | 10090.ENSMUSP00000104543 |
Miscellaneous databases
RNActi | Q8BYK5, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 92 – 117 | RPEL 1Add BLAST | 26 | |
Repeati | 400 – 425 | RPEL 2Add BLAST | 26 | |
Repeati | 438 – 463 | RPEL 3Add BLAST | 26 | |
Repeati | 476 – 501 | RPEL 4Add BLAST | 26 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 449 – 485 | Sequence analysisAdd BLAST | 37 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 216 – 238 | Pro-richAdd BLAST | 23 |
Sequence similaritiesi
Belongs to the phosphatase and actin regulator family.Curated
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG4339, Eukaryota |
GeneTreei | ENSGT00940000157562 |
HOGENOMi | CLU_015753_3_1_1 |
InParanoidi | Q8BYK5 |
OMAi | EDCVLQR |
OrthoDBi | 1205245at2759 |
TreeFami | TF316316 |
Family and domain databases
InterProi | View protein in InterPro IPR029990, Phactr3 IPR004018, RPEL_repeat |
PANTHERi | PTHR12751:SF7, PTHR12751:SF7, 1 hit |
Pfami | View protein in Pfam PF02755, RPEL, 1 hit |
SMARTi | View protein in SMART SM00707, RPEL, 4 hits |
PROSITEi | View protein in PROSITE PS51073, RPEL, 4 hits |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8BYK5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAASEDGSSC LVSRGRSQSD PSFLSDSSAT STDAGENPDE MDQTPPARSE
60 70 80 90 100
PLVSGIRTPP VRRNSKLATL GRIFKPWKWR KKKNEKLKQT TSALEKKMAG
110 120 130 140 150
RQGREELIKQ GLLEMMEQDS ENKACSPKEG SQPVQSEPPA GEQETLTSEG
160 170 180 190 200
AQPGSPSASG TDQVSQDELL SSDAHLDDTA NIPSASTAEE ADAGSLLPTT
210 220 230 240 250
DEPSQALAGS DSLDSPPRSL ERSVSQLPSP PLLPTPPPKA SSKATKNVTG
260 270 280 290 300
QAALFQGPSM KNNEPALRGQ LATPTGSPHV TTVHRPLPPS RVMEELHRAL
310 320 330 340 350
ATKHRQDSFQ GRECRGSPKK RMDVRLSRTS SMERGKERDE AWSFDGASEN
360 370 380 390 400
KWTATKDSEE NKENLMLSSE LKDDMLLYQD EEALNDSIIS GTLPRKCKKE
410 420 430 440 450
LLAVKLRNRP SKQELEDRNI FPRRTDEERQ EIRQQIEMKL SKRLSQRPAV
460 470 480 490 500
EELERRNILK QRNDQTEQEE RREIKQRLTR KLNQRPTVDE LRDRKILIRF
510 520 530 540 550
SDYVEVARAQ DYDRRADKPW TRLSAADKAA IRKELNEYKS NEMEVHASSK
HLTRFHRP
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2AHM4 | A2AHM4_MOUSE | Phosphatase and actin regulator | Phactr3 | 553 | Annotation score: | ||
A0A5F8MPC1 | A0A5F8MPC1_MOUSE | Phosphatase and actin regulator | Phactr3 | 128 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009095 | 1 – 40 | Missing in isoform 4. 1 PublicationAdd BLAST | 40 | |
Alternative sequenceiVSP_009093 | 1 – 38 | MAASE…AGENP → MQTANQMLSLNFRRMKSGTA AVRTRARHRPPGSGPSRCG in isoform 2. 1 PublicationAdd BLAST | 38 | |
Alternative sequenceiVSP_009094 | 1 – 38 | MAASE…AGENP → MRGQGRGHARWPAPLRSLLR AFGPQDTTTGGLEQ in isoform 3. 1 PublicationAdd BLAST | 38 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY501387 mRNA Translation: AAS86433.1 AK005136 mRNA Translation: BAB23834.1 AK032242 mRNA Translation: BAC27776.1 AK038449 mRNA Translation: BAC30003.1 AK039213 mRNA Translation: BAC30278.1 BC058621 mRNA Translation: AAH58621.1 BC059869 mRNA Translation: AAH59869.1 |
CCDSi | CCDS17158.1 [Q8BYK5-1] CCDS17159.1 [Q8BYK5-4] CCDS50837.1 [Q8BYK5-3] CCDS50839.1 [Q8BYK5-2] |
RefSeqi | NP_001007155.1, NM_001007154.3 [Q8BYK5-4] NP_001171260.1, NM_001177789.1 [Q8BYK5-3] NP_001171261.1, NM_001177790.1 NP_001171262.1, NM_001177791.1 [Q8BYK5-2] NP_083082.1, NM_028806.2 [Q8BYK5-1] XP_006500793.1, XM_006500730.2 [Q8BYK5-4] |
Genome annotation databases
Ensembli | ENSMUST00000103065; ENSMUSP00000099354; ENSMUSG00000027525 [Q8BYK5-4] ENSMUST00000103066; ENSMUSP00000099355; ENSMUSG00000027525 [Q8BYK5-1] ENSMUST00000108915; ENSMUSP00000104543; ENSMUSG00000027525 [Q8BYK5-2] ENSMUST00000108916; ENSMUSP00000104544; ENSMUSG00000027525 [Q8BYK5-3] |
GeneIDi | 74189 |
KEGGi | mmu:74189 |
UCSCi | uc008ohj.2, mouse [Q8BYK5-3] uc008ohk.2, mouse [Q8BYK5-1] uc008ohl.2, mouse [Q8BYK5-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY501387 mRNA Translation: AAS86433.1 AK005136 mRNA Translation: BAB23834.1 AK032242 mRNA Translation: BAC27776.1 AK038449 mRNA Translation: BAC30003.1 AK039213 mRNA Translation: BAC30278.1 BC058621 mRNA Translation: AAH58621.1 BC059869 mRNA Translation: AAH59869.1 |
CCDSi | CCDS17158.1 [Q8BYK5-1] CCDS17159.1 [Q8BYK5-4] CCDS50837.1 [Q8BYK5-3] CCDS50839.1 [Q8BYK5-2] |
RefSeqi | NP_001007155.1, NM_001007154.3 [Q8BYK5-4] NP_001171260.1, NM_001177789.1 [Q8BYK5-3] NP_001171261.1, NM_001177790.1 NP_001171262.1, NM_001177791.1 [Q8BYK5-2] NP_083082.1, NM_028806.2 [Q8BYK5-1] XP_006500793.1, XM_006500730.2 [Q8BYK5-4] |
3D structure databases
SMRi | Q8BYK5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 216561, 2 interactors |
STRINGi | 10090.ENSMUSP00000104543 |
PTM databases
iPTMneti | Q8BYK5 |
PhosphoSitePlusi | Q8BYK5 |
Proteomic databases
PaxDbi | Q8BYK5 |
PRIDEi | Q8BYK5 |
Protocols and materials databases
Antibodypediai | 29289, 111 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000103065; ENSMUSP00000099354; ENSMUSG00000027525 [Q8BYK5-4] ENSMUST00000103066; ENSMUSP00000099355; ENSMUSG00000027525 [Q8BYK5-1] ENSMUST00000108915; ENSMUSP00000104543; ENSMUSG00000027525 [Q8BYK5-2] ENSMUST00000108916; ENSMUSP00000104544; ENSMUSG00000027525 [Q8BYK5-3] |
GeneIDi | 74189 |
KEGGi | mmu:74189 |
UCSCi | uc008ohj.2, mouse [Q8BYK5-3] uc008ohk.2, mouse [Q8BYK5-1] uc008ohl.2, mouse [Q8BYK5-2] |
Organism-specific databases
CTDi | 116154 |
MGIi | MGI:1921439, Phactr3 |
Phylogenomic databases
eggNOGi | KOG4339, Eukaryota |
GeneTreei | ENSGT00940000157562 |
HOGENOMi | CLU_015753_3_1_1 |
InParanoidi | Q8BYK5 |
OMAi | EDCVLQR |
OrthoDBi | 1205245at2759 |
TreeFami | TF316316 |
Miscellaneous databases
BioGRID-ORCSi | 74189, 0 hits in 17 CRISPR screens |
PROi | PR:Q8BYK5 |
RNActi | Q8BYK5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027525, Expressed in lumbar subsegment of spinal cord and 150 other tissues |
ExpressionAtlasi | Q8BYK5, baseline and differential |
Genevisiblei | Q8BYK5, MM |
Family and domain databases
InterProi | View protein in InterPro IPR029990, Phactr3 IPR004018, RPEL_repeat |
PANTHERi | PTHR12751:SF7, PTHR12751:SF7, 1 hit |
Pfami | View protein in Pfam PF02755, RPEL, 1 hit |
SMARTi | View protein in SMART SM00707, RPEL, 4 hits |
PROSITEi | View protein in PROSITE PS51073, RPEL, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHAR3_MOUSE | |
Accessioni | Q8BYK5Primary (citable) accession number: Q8BYK5 Secondary accession number(s): Q8BYS8, Q8C058, Q9DB87 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2003 |
Last sequence update: | November 28, 2003 | |
Last modified: | December 2, 2020 | |
This is version 126 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families