UniProtKB - Q8BY02 (NKRF_MOUSE)
Protein
NF-kappa-B-repressing factor
Gene
Nkrf
Organism
Mus musculus (Mouse)
Status
Functioni
Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 296 – 388 | By similarityAdd BLAST | 93 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- negative regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: NF-kappa-B-repressing factorShort name: NFkB-repressing factor Alternative name(s): Transcription factor NRF |
Gene namesi | Name:Nkrf Synonyms:Nrf |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1924536, Nkrf |
Subcellular locationi
Nucleus
- nucleolus By similarity
Nucleus
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096870 | 1 – 690 | NF-kappa-B-repressing factorAdd BLAST | 690 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 68 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 500 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 618 | PhosphoserineBy similarity | 1 | |
Cross-linki | 666 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 674 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8BY02 |
MaxQBi | Q8BY02 |
PaxDbi | Q8BY02 |
PeptideAtlasi | Q8BY02 |
PRIDEi | Q8BY02 |
PTM databases
PhosphoSitePlusi | Q8BY02 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000044149, Expressed in lumbar subsegment of spinal cord and 255 other tissues |
Genevisiblei | Q8BY02, MM |
Interactioni
Subunit structurei
Interacts with NF-kappa-B.
Interacts with XRN2.
By similarityProtein-protein interaction databases
BioGRIDi | 218599, 6 interactors |
IntActi | Q8BY02, 1 interactor |
STRINGi | 10090.ENSMUSP00000061546 |
Miscellaneous databases
RNActi | Q8BY02, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 551 – 596 | G-patchPROSITE-ProRule annotationAdd BLAST | 46 | |
Domaini | 600 – 664 | R3HPROSITE-ProRule annotationAdd BLAST | 65 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 296 | Active repression domainBy similarityAdd BLAST | 296 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 25 – 45 | Nuclear localization signalBy similarityAdd BLAST | 21 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000157256 |
HOGENOMi | CLU_025268_0_0_1 |
InParanoidi | Q8BY02 |
OMAi | SSQCHSG |
OrthoDBi | 1179335at2759 |
TreeFami | TF326321 |
Family and domain databases
CDDi | cd02640, R3H_NRF, 1 hit |
Gene3Di | 3.30.1370.50, 1 hit |
InterProi | View protein in InterPro IPR000467, G_patch_dom IPR001374, R3H_dom IPR036867, R3H_dom_sf IPR034071, R3H_NRF |
Pfami | View protein in Pfam PF01585, G-patch, 1 hit PF01424, R3H, 1 hit |
SMARTi | View protein in SMART SM00443, G_patch, 1 hit SM00393, R3H, 1 hit |
SUPFAMi | SSF82708, SSF82708, 1 hit |
PROSITEi | View protein in PROSITE PS50174, G_PATCH, 1 hit PS51061, R3H, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8BY02-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEKILHMAEG IDIGEMPSYD LMLPKPSKGQ KRYLSTYDGQ NPPKKQAGSK
60 70 80 90 100
FHVRARFEPV HFVASSSKAE RQEDPYGPQT KDVNGRTHFA SMPRNFYQDY
110 120 130 140 150
TQDSFSIQDG NSQYCNSSGF IFTKDQPVAT NMYFDSGNPA PSSTSQQANC
160 170 180 190 200
QPAPEPPPSQ MYPESLVAEK QYFIEKLTAT IWKNLSNPEM TSGSDKINYT
210 220 230 240 250
YMLTRCIQAC KTNPEYIYAP LKEIPPADIP KNKKLLTDGY ACEVRCQNIY
260 270 280 290 300
LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHI IGEDLVVCQI
310 320 330 340 350
GMLSYEFPPA LKPPEDLVVL GKDASGQPIF NSSAKHWTNF VITENANDAI
360 370 380 390 400
GILNNSASFN KMSIEYKYEM MPNRTWRCRV FLQDHCLAEG YGTKKTSKHA
410 420 430 440 450
AADEALKVLQ KTQPTYPSVK SSQCHSGSSP KGSGKKKDIK DLVVYENSSN
460 470 480 490 500
PVCTLNDTAQ FNRMTVEYVY ERMTGLRWKC KVILESEVIA EAVGVKKSVK
510 520 530 540 550
YEAAGEAVKT LKKTQPTVIN NLKKGTVEDV ISRNEIQGRS AEEAYKQQIK
560 570 580 590 600
EDNIGNQLLR KMGWTGGGLG KSGEGIREPI SVKEQHKREG LGLDVERVNK
610 620 630 640 650
IAKRDIEQII RNYARSESHS DLTFSTELTN DERKQIHQIA QKYGLKSKSH
660 670 680 690
GVGHDRYLVV GRKRRKEDLL DQLKQEGQVG HYELVMPQAN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A668KL68 | A0A668KL68_MOUSE | NF-kappa-B-repressing factor | Nkrf | 784 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 686 | M → V in BAC37550 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK020455 mRNA Translation: BAB32107.1 AK042690 mRNA Translation: BAC31334.1 AK079123 mRNA Translation: BAC37550.1 AL450399 Genomic DNA No translation available. BC138828 mRNA Translation: AAI38829.1 BC138829 mRNA Translation: AAI38830.1 |
RefSeqi | NP_084167.2, NM_029891.2 |
Genome annotation databases
Ensembli | ENSMUST00000057093; ENSMUSP00000061546; ENSMUSG00000044149 |
GeneIDi | 77286 |
KEGGi | mmu:77286 |
UCSCi | uc009sxw.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK020455 mRNA Translation: BAB32107.1 AK042690 mRNA Translation: BAC31334.1 AK079123 mRNA Translation: BAC37550.1 AL450399 Genomic DNA No translation available. BC138828 mRNA Translation: AAI38829.1 BC138829 mRNA Translation: AAI38830.1 |
RefSeqi | NP_084167.2, NM_029891.2 |
3D structure databases
SMRi | Q8BY02 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 218599, 6 interactors |
IntActi | Q8BY02, 1 interactor |
STRINGi | 10090.ENSMUSP00000061546 |
PTM databases
PhosphoSitePlusi | Q8BY02 |
Proteomic databases
EPDi | Q8BY02 |
MaxQBi | Q8BY02 |
PaxDbi | Q8BY02 |
PeptideAtlasi | Q8BY02 |
PRIDEi | Q8BY02 |
Protocols and materials databases
Antibodypediai | 462, 195 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000057093; ENSMUSP00000061546; ENSMUSG00000044149 |
GeneIDi | 77286 |
KEGGi | mmu:77286 |
UCSCi | uc009sxw.1, mouse |
Organism-specific databases
CTDi | 55922 |
MGIi | MGI:1924536, Nkrf |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000157256 |
HOGENOMi | CLU_025268_0_0_1 |
InParanoidi | Q8BY02 |
OMAi | SSQCHSG |
OrthoDBi | 1179335at2759 |
TreeFami | TF326321 |
Miscellaneous databases
BioGRID-ORCSi | 77286, 0 hits in 17 CRISPR screens |
ChiTaRSi | Nkrf, mouse |
PROi | PR:Q8BY02 |
RNActi | Q8BY02, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000044149, Expressed in lumbar subsegment of spinal cord and 255 other tissues |
Genevisiblei | Q8BY02, MM |
Family and domain databases
CDDi | cd02640, R3H_NRF, 1 hit |
Gene3Di | 3.30.1370.50, 1 hit |
InterProi | View protein in InterPro IPR000467, G_patch_dom IPR001374, R3H_dom IPR036867, R3H_dom_sf IPR034071, R3H_NRF |
Pfami | View protein in Pfam PF01585, G-patch, 1 hit PF01424, R3H, 1 hit |
SMARTi | View protein in SMART SM00443, G_patch, 1 hit SM00393, R3H, 1 hit |
SUPFAMi | SSF82708, SSF82708, 1 hit |
PROSITEi | View protein in PROSITE PS50174, G_PATCH, 1 hit PS51061, R3H, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NKRF_MOUSE | |
Accessioni | Q8BY02Primary (citable) accession number: Q8BY02 Secondary accession number(s): A2A3V7, Q8BJU5, Q9CRL2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 15, 2003 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 147 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot