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Protein

GPI transamidase component PIG-T

Gene

Pigt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates (By similarity).By similarity

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Biological processi

  • attachment of GPI anchor to protein Source: GO_Central
  • neuron apoptotic process Source: MGI
  • neuron differentiation Source: MGI

Keywordsi

Biological processGPI-anchor biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-162791 Attachment of GPI anchor to uPAR
UniPathwayi
UPA00196

Names & Taxonomyi

Protein namesi
Recommended name:
GPI transamidase component PIG-T
Alternative name(s):
Neuronal development-associated protein 7
Phosphatidylinositol-glycan biosynthesis class T protein
Gene namesi
Name:Pigt
Synonyms:Ndap7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1926178 Pigt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 525LumenalSequence analysisAdd BLAST500
Transmembranei526 – 548HelicalSequence analysisAdd BLAST23
Topological domaini549 – 582CytoplasmicSequence analysisAdd BLAST34

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000002410826 – 582GPI transamidase component PIG-TAdd BLAST557

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi168N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi186Interchain (with C-92 in PIGK/GPI8)By similarity
Glycosylationi295N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi331N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ8BXQ2
MaxQBiQ8BXQ2
PaxDbiQ8BXQ2
PRIDEiQ8BXQ2

PTM databases

iPTMnetiQ8BXQ2
PhosphoSitePlusiQ8BXQ2
SwissPalmiQ8BXQ2

Expressioni

Gene expression databases

BgeeiENSMUSG00000017721 Expressed in 106 organ(s), highest expression level in colon
ExpressionAtlasiQ8BXQ2 baseline and differential
GenevisibleiQ8BXQ2 MM

Interactioni

Subunit structurei

Forms a complex with PIGK/GPI8, PIGS, PIGU and GPAA1/GAA1. Has a critical role in maintaining the complex by stabilizing the expression of GPAA1 and GPI8 and linking them to PIGS (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8BXQ2, 1 interactor
MINTiQ8BXQ2
STRINGi10090.ENSMUSP00000099390

Structurei

3D structure databases

ProteinModelPortaliQ8BXQ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGT family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2407 Eukaryota
ENOG410XRR0 LUCA
GeneTreeiENSGT00390000018558
HOGENOMiHOG000215945
HOVERGENiHBG049395
InParanoidiQ8BXQ2
KOiK05292
OMAiDANHGHY
OrthoDBiEOG091G06DH
PhylomeDBiQ8BXQ2
TreeFamiTF105921

Family and domain databases

InterProiView protein in InterPro
IPR007245 PIG-T
PANTHERiPTHR12959 PTHR12959, 1 hit
PfamiView protein in Pfam
PF04113 Gpi16, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q8BXQ2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAAMPLGLP LRLLVLLLVG RGCCGCAEGP RDSLREELVI TPLPSGDVAA
60 70 80 90 100
TFQFRTRWDS DLQREGVSHY RLFPKALGQL ISKYSLRELH LSFTQGFWRT
110 120 130 140 150
RYWGPPFLQA PSGAELWVWF QDTVTDVDKS WRELSNVLSG IFCASLNFID
160 170 180 190 200
ATNTVTPTAS FKPLGLANDT DDYFLRYAVL PREVVCTENL TPWKKLLPCS
210 220 230 240 250
SKAGLSVLLK ADRLFHTSYH SQAVHIRPIC RNAHCTSISW ELRQTLSVVF
260 270 280 290 300
DAFITGQGKK DWSLFRMFSR TLTEACPLAS QSLVYVDITG YSQDNETLEV
310 320 330 340 350
SPPPTSTYQD VILGTRKTYA VYDLFDTAMI NNSRNLNIQL KWKRPPDNEA
360 370 380 390 400
LPVPFLHAQR YVSGYGLQKG ELSTLLYNSH PYRAFPVLLL DVVPWYLRLY
410 420 430 440 450
VHTLTITSKG KENKPSYIHY QPAQDRQQPH LLEMLIQLPA NSVTKVSIQF
460 470 480 490 500
ERALLKWTEY TPDPNHGFYV SPSVLSALVP SVVAAKPVDW EGSPLFNTLF
510 520 530 540 550
PVSDGSSYFV RLYTEPLLVN LPTPDFSMPY NVICLTCTVV AVCYGSFYNL
560 570 580
LTRTFHIEEP KSGGLAKRLA NLIRRARGVP PL
Length:582
Mass (Da):65,705
Last modified:March 1, 2004 - v2
Checksum:i39FF3CF98D3F9322
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7ZC19B7ZC19_MOUSE
GPI transamidase component PIG-T
Pigt
435Annotation score:
F6U4X6F6U4X6_MOUSE
GPI transamidase component PIG-T
Pigt
143Annotation score:

Sequence cautioni

The sequence AAH34175 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAK32809 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB39711 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAC34729 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti346P → Q in BAC31951 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044499 mRNA Translation: BAC31951.1
AK155057 mRNA Translation: BAE33018.1
AL591478 Genomic DNA No translation available.
AJ308886 mRNA Translation: CAC34729.1 Different initiation.
AF361435 mRNA Translation: AAK32809.1 Different initiation.
AB057593 mRNA Translation: BAB39711.1 Different initiation.
BC034175 mRNA Translation: AAH34175.1 Different initiation.
CCDSiCCDS17038.2
RefSeqiNP_598540.2, NM_133779.2
XP_006500484.2, XM_006500421.3
UniGeneiMm.28228

Genome annotation databases

EnsembliENSMUST00000103101; ENSMUSP00000099390; ENSMUSG00000017721
GeneIDi78928
KEGGimmu:78928
UCSCiuc008nvc.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044499 mRNA Translation: BAC31951.1
AK155057 mRNA Translation: BAE33018.1
AL591478 Genomic DNA No translation available.
AJ308886 mRNA Translation: CAC34729.1 Different initiation.
AF361435 mRNA Translation: AAK32809.1 Different initiation.
AB057593 mRNA Translation: BAB39711.1 Different initiation.
BC034175 mRNA Translation: AAH34175.1 Different initiation.
CCDSiCCDS17038.2
RefSeqiNP_598540.2, NM_133779.2
XP_006500484.2, XM_006500421.3
UniGeneiMm.28228

3D structure databases

ProteinModelPortaliQ8BXQ2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BXQ2, 1 interactor
MINTiQ8BXQ2
STRINGi10090.ENSMUSP00000099390

PTM databases

iPTMnetiQ8BXQ2
PhosphoSitePlusiQ8BXQ2
SwissPalmiQ8BXQ2

Proteomic databases

EPDiQ8BXQ2
MaxQBiQ8BXQ2
PaxDbiQ8BXQ2
PRIDEiQ8BXQ2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103101; ENSMUSP00000099390; ENSMUSG00000017721
GeneIDi78928
KEGGimmu:78928
UCSCiuc008nvc.1 mouse

Organism-specific databases

CTDi51604
MGIiMGI:1926178 Pigt

Phylogenomic databases

eggNOGiKOG2407 Eukaryota
ENOG410XRR0 LUCA
GeneTreeiENSGT00390000018558
HOGENOMiHOG000215945
HOVERGENiHBG049395
InParanoidiQ8BXQ2
KOiK05292
OMAiDANHGHY
OrthoDBiEOG091G06DH
PhylomeDBiQ8BXQ2
TreeFamiTF105921

Enzyme and pathway databases

UniPathwayi
UPA00196

ReactomeiR-MMU-162791 Attachment of GPI anchor to uPAR

Miscellaneous databases

PROiPR:Q8BXQ2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017721 Expressed in 106 organ(s), highest expression level in colon
ExpressionAtlasiQ8BXQ2 baseline and differential
GenevisibleiQ8BXQ2 MM

Family and domain databases

InterProiView protein in InterPro
IPR007245 PIG-T
PANTHERiPTHR12959 PTHR12959, 1 hit
PfamiView protein in Pfam
PF04113 Gpi16, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPIGT_MOUSE
AccessioniPrimary (citable) accession number: Q8BXQ2
Secondary accession number(s): A2A5G2, Q3U2X2, Q99JA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: November 7, 2018
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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