UniProtKB - Q8BXK9 (CLIC5_MOUSE)
Protein
Chloride intracellular channel protein 5
Gene
Clic5
Organism
Mus musculus (Mouse)
Status
Functioni
Required for normal hearing (By similarity). It is necessary for the formation of stereocilia in the inner ear and normal development of the organ of Corti. Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture (PubMed:17021174). Plays a role in formation of the lens suture in the eye, which is important for normal optical properties of the lens (PubMed:29425878).By similarity2 Publications
GO - Molecular functioni
- chloride channel activity Source: MGI
- voltage-gated ion channel activity Source: UniProtKB-KW
GO - Biological processi
- auditory receptor cell stereocilium organization Source: MGI
- chloride transport Source: MGI
- diet induced thermogenesis Source: MGI
- neuromuscular process controlling balance Source: MGI
- protein localization Source: MGI
- regulation of ion transmembrane transport Source: UniProtKB-KW
- response to dietary excess Source: MGI
- sensory perception of sound Source: MGI
- visual perception Source: UniProtKB-KW
Keywordsi
Molecular function | Chloride channel, Ion channel, Voltage-gated channel |
Biological process | Hearing, Ion transport, Sensory transduction, Transport, Vision |
Ligand | Chloride |
Names & Taxonomyi
Protein namesi | Recommended name: Chloride intracellular channel protein 5 |
Gene namesi | Name:Clic5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1917912, Clic5 |
Subcellular locationi
Plasma membrane
- Apical cell membrane 1 Publication; Single-pass membrane protein Sequence analysis
Golgi apparatus
- Golgi apparatus By similarity
Cytoskeleton
- centrosome 1 Publication
- cytoskeleton By similarity
Other locations
- cell cortex By similarity
- Membrane By similarity; Single-pass membrane protein Sequence analysis
- Cytoplasm By similarity
Note: Colocalized with AKAP9 at the Golgi apparatus as well as, to a lesser extent, the centrosome (By similarity). Associates with the cortical actin cytoskeleton (By similarity). Localizes to the apical region of cochlear hair cells, at the base of the actin-rich hair bundle (PubMed:17021174). Colocalizes with podocalyxin at the apical cell membrane in renal glomeruli (By similarity). May localize to the centrosome in lens epithelial cells (PubMed:29425878). Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (By similarity).By similarity2 Publications
Cytoskeleton
- actin cytoskeleton Source: UniProtKB
- microtubule organizing center Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: MGI
Nucleus
- nucleus Source: GO_Central
Plasma Membrane
- apical plasma membrane Source: UniProtKB-SubCell
Other locations
- cell cortex Source: UniProtKB-SubCell
- chloride channel complex Source: UniProtKB-KW
- stereocilium Source: MGI
- stereocilium bundle Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 34 – 54 | Helical; Note=After insertion into the membraneSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, MembranePathology & Biotechi
Involvement in diseasei
Defects in Clic5 are a cause of the jitterbug (jbg) phenotype. Jbg is the result of a spontaneous mutation that leads to severe degeneration of the organ of Corti in the inner ear. Jbg leads to progressive degeneration of inner ear hair cells. Affected mice are identified by head bobbing, circling behavior and their inability to swim. They cannot hear well when young, and become completely deaf after 5 months (PubMed:17021174). In addition, there are eye defects with aberrant development of the lens suture and formation of lens opacities (PubMed:29425878).2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000144215 | 1 – 251 | Chloride intracellular channel protein 5Add BLAST | 251 |
Proteomic databases
EPDi | Q8BXK9 |
jPOSTi | Q8BXK9 |
MaxQBi | Q8BXK9 |
PaxDbi | Q8BXK9 |
PeptideAtlasi | Q8BXK9 |
PRIDEi | Q8BXK9 |
PTM databases
iPTMneti | Q8BXK9 |
PhosphoSitePlusi | Q8BXK9 |
Expressioni
Tissue specificityi
Detected in lung and inner ear. Detected in embryonic cochlea, on microvilli-covered apical surfaces of interdental cells, columnar cells of Kolliker's organ, and on stereocilia of inner and outer hair cells (at protein level) (PubMed:17021174). Also detected in the eye, where it localizes to lens fiber cells in the lens epithelium (at protein level) (PubMed:29425878).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000023959, Expressed in intestine and 172 other tissues |
Genevisiblei | Q8BXK9, MM |
Interactioni
Subunit structurei
Component of a multimeric complex consisting of several cytoskeletal proteins, including actin, ezrin, alpha-actinin, gelsolin, and IQGAP1.
Interacts with AKAP9 (By similarity).
By similarityProtein-protein interaction databases
IntActi | Q8BXK9, 4 interactors |
MINTi | Q8BXK9 |
STRINGi | 10090.ENSMUSP00000024755 |
Miscellaneous databases
RNActi | Q8BXK9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 101 – 241 | GST C-terminalAdd BLAST | 141 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 98 | Required for insertion into the membraneBy similarityAdd BLAST | 98 |
Domaini
Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion (By similarity).By similarity
Sequence similaritiesi
Belongs to the chloride channel CLIC family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1422, Eukaryota |
GeneTreei | ENSGT00940000156406 |
HOGENOMi | CLU_061051_1_0_1 |
InParanoidi | Q8BXK9 |
OMAi | FTNQRET |
OrthoDBi | 974249at2759 |
PhylomeDBi | Q8BXK9 |
TreeFami | TF315438 |
Family and domain databases
CDDi | cd10297, GST_C_CLIC5, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR002946, CLIC IPR030264, CLIC-5 IPR042069, CLIC5_C_GST IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR040079, Glutathione_S-Trfase IPR004045, Glutathione_S-Trfase_N IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR45476:SF4, PTHR45476:SF4, 1 hit |
Pfami | View protein in Pfam PF13409, GST_N_2, 1 hit |
PRINTSi | PR01263, INTCLCHANNEL |
SFLDi | SFLDS00019, Glutathione_Transferase_(cytos, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR00862, O-ClC, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8BXK9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDSATTNGD DRDPEIELFV KAGIDGESIG NCPFSQRLFM ILWLKGVVFN
60 70 80 90 100
VTTVDLKRKP ADLHNLAPGT HPPFLTFNGD VKTDVNKIEE FLEETLTPEK
110 120 130 140 150
YPKLAAKHRE SNTAGIDIFS KFSAYIKNTK QQNNAALERG LTKALRKLDD
160 170 180 190 200
YLNSPLPEEI DTNTHGDEKG SQRKFLDGDE LTLADCNLLP KLHVVKIVAK
210 220 230 240 250
KYRNYDIPAE MTGLWRYLKN AYARDEFTNT CAADSEIELA YADVARRLSR
S
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A668KLB7 | A0A668KLB7_MOUSE | Chloride intracellular channel prot... | Clic5 | 485 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK046522 mRNA Translation: BAC32769.1 AK156849 mRNA Translation: BAE33875.1 BC064037 mRNA Translation: AAH64037.1 |
CCDSi | CCDS28804.1 |
RefSeqi | NP_766209.1, NM_172621.2 |
Genome annotation databases
Ensembli | ENSMUST00000024755; ENSMUSP00000024755; ENSMUSG00000023959 |
GeneIDi | 224796 |
KEGGi | mmu:224796 |
UCSCi | uc008cpw.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK046522 mRNA Translation: BAC32769.1 AK156849 mRNA Translation: BAE33875.1 BC064037 mRNA Translation: AAH64037.1 |
CCDSi | CCDS28804.1 |
RefSeqi | NP_766209.1, NM_172621.2 |
3D structure databases
SMRi | Q8BXK9 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q8BXK9, 4 interactors |
MINTi | Q8BXK9 |
STRINGi | 10090.ENSMUSP00000024755 |
PTM databases
iPTMneti | Q8BXK9 |
PhosphoSitePlusi | Q8BXK9 |
Proteomic databases
EPDi | Q8BXK9 |
jPOSTi | Q8BXK9 |
MaxQBi | Q8BXK9 |
PaxDbi | Q8BXK9 |
PeptideAtlasi | Q8BXK9 |
PRIDEi | Q8BXK9 |
Protocols and materials databases
Antibodypediai | 16850, 243 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000024755; ENSMUSP00000024755; ENSMUSG00000023959 |
GeneIDi | 224796 |
KEGGi | mmu:224796 |
UCSCi | uc008cpw.1, mouse |
Organism-specific databases
CTDi | 53405 |
MGIi | MGI:1917912, Clic5 |
Phylogenomic databases
eggNOGi | KOG1422, Eukaryota |
GeneTreei | ENSGT00940000156406 |
HOGENOMi | CLU_061051_1_0_1 |
InParanoidi | Q8BXK9 |
OMAi | FTNQRET |
OrthoDBi | 974249at2759 |
PhylomeDBi | Q8BXK9 |
TreeFami | TF315438 |
Miscellaneous databases
BioGRID-ORCSi | 224796, 1 hit in 18 CRISPR screens |
ChiTaRSi | Clic5, mouse |
PROi | PR:Q8BXK9 |
RNActi | Q8BXK9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000023959, Expressed in intestine and 172 other tissues |
Genevisiblei | Q8BXK9, MM |
Family and domain databases
CDDi | cd10297, GST_C_CLIC5, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR002946, CLIC IPR030264, CLIC-5 IPR042069, CLIC5_C_GST IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR040079, Glutathione_S-Trfase IPR004045, Glutathione_S-Trfase_N IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR45476:SF4, PTHR45476:SF4, 1 hit |
Pfami | View protein in Pfam PF13409, GST_N_2, 1 hit |
PRINTSi | PR01263, INTCLCHANNEL |
SFLDi | SFLDS00019, Glutathione_Transferase_(cytos, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR00862, O-ClC, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CLIC5_MOUSE | |
Accessioni | Q8BXK9Primary (citable) accession number: Q8BXK9 Secondary accession number(s): Q3U0H8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families