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Protein

Chloride intracellular channel protein 5

Gene

Clic5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for normal hearing (By similarity). It is necessary for the formation of stereocilia in the inner ear and normal development of the organ of Corti. Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture (PubMed:17021174).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChloride channel, Ion channel, Voltage-gated channel
Biological processHearing, Ion transport, Transport
LigandChloride

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride intracellular channel protein 5
Gene namesi
Name:Clic5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1917912 Clic5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei34 – 54Helical; Note=After insertion into the membraneSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Clic5 are a cause of the jitterbug (jbg) phenotype. Jbg is the result of a spontaneous mutation that leads to severe degeneration of the organ of Corti in the inner ear. Jbg leads to progressive degeneration of inner ear hair cells. Affected mice are identified by head bobbing, circling behavior and their inability to swim. They cannot hear well when young, and become completely deaf after 5 months.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001442151 – 251Chloride intracellular channel protein 5Add BLAST251

Proteomic databases

EPDiQ8BXK9
MaxQBiQ8BXK9
PaxDbiQ8BXK9
PeptideAtlasiQ8BXK9
PRIDEiQ8BXK9

PTM databases

iPTMnetiQ8BXK9
PhosphoSitePlusiQ8BXK9

Expressioni

Tissue specificityi

Detected in lung and inner ear. Detected in embryonic cochlea, on microvilli-covered apical surfaces of interdental cells, columnar cells of Kolliker's organ, and on stereocilia of inner and outer hair cells (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000023959 Expressed in 155 organ(s), highest expression level in lung
CleanExiMM_CLIC5
GenevisibleiQ8BXK9 MM

Interactioni

Subunit structurei

Component of a multimeric complex consisting of several cytoskeletal proteins, including actin, ezrin, alpha-actinin, gelsolin, and IQGAP1. Interacts with AKAP9 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8BXK9, 4 interactors
MINTiQ8BXK9
STRINGi10090.ENSMUSP00000024755

Structurei

3D structure databases

ProteinModelPortaliQ8BXK9
SMRiQ8BXK9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 241GST C-terminalAdd BLAST141

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 98Required for insertion into the membraneBy similarityAdd BLAST98

Domaini

Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion (By similarity).By similarity

Sequence similaritiesi

Belongs to the chloride channel CLIC family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1422 Eukaryota
ENOG410ZRK6 LUCA
GeneTreeiENSGT00550000074477
HOGENOMiHOG000231548
HOVERGENiHBG050994
InParanoidiQ8BXK9
KOiK05025
OMAiGAICKAG
OrthoDBiEOG091G0IHT
PhylomeDBiQ8BXK9
TreeFamiTF315438

Family and domain databases

InterProiView protein in InterPro
IPR002946 CLIC
IPR030264 CLIC-5
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR036249 Thioredoxin-like_sf
PANTHERiPTHR43920:SF6 PTHR43920:SF6, 1 hit
PfamiView protein in Pfam
PF13417 GST_N_3, 1 hit
PRINTSiPR01263 INTCLCHANNEL
SFLDiSFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
TIGRFAMsiTIGR00862 O-ClC, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BXK9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTDSATTNGD DRDPEIELFV KAGIDGESIG NCPFSQRLFM ILWLKGVVFN
60 70 80 90 100
VTTVDLKRKP ADLHNLAPGT HPPFLTFNGD VKTDVNKIEE FLEETLTPEK
110 120 130 140 150
YPKLAAKHRE SNTAGIDIFS KFSAYIKNTK QQNNAALERG LTKALRKLDD
160 170 180 190 200
YLNSPLPEEI DTNTHGDEKG SQRKFLDGDE LTLADCNLLP KLHVVKIVAK
210 220 230 240 250
KYRNYDIPAE MTGLWRYLKN AYARDEFTNT CAADSEIELA YADVARRLSR

S
Length:251
Mass (Da):28,287
Last modified:March 1, 2003 - v1
Checksum:i7055A17D22977974
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046522 mRNA Translation: BAC32769.1
AK156849 mRNA Translation: BAE33875.1
BC064037 mRNA Translation: AAH64037.1
CCDSiCCDS28804.1
RefSeqiNP_766209.1, NM_172621.2
UniGeneiMm.37666

Genome annotation databases

EnsembliENSMUST00000024755; ENSMUSP00000024755; ENSMUSG00000023959
GeneIDi224796
KEGGimmu:224796
UCSCiuc008cpw.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046522 mRNA Translation: BAC32769.1
AK156849 mRNA Translation: BAE33875.1
BC064037 mRNA Translation: AAH64037.1
CCDSiCCDS28804.1
RefSeqiNP_766209.1, NM_172621.2
UniGeneiMm.37666

3D structure databases

ProteinModelPortaliQ8BXK9
SMRiQ8BXK9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BXK9, 4 interactors
MINTiQ8BXK9
STRINGi10090.ENSMUSP00000024755

PTM databases

iPTMnetiQ8BXK9
PhosphoSitePlusiQ8BXK9

Proteomic databases

EPDiQ8BXK9
MaxQBiQ8BXK9
PaxDbiQ8BXK9
PeptideAtlasiQ8BXK9
PRIDEiQ8BXK9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024755; ENSMUSP00000024755; ENSMUSG00000023959
GeneIDi224796
KEGGimmu:224796
UCSCiuc008cpw.1 mouse

Organism-specific databases

CTDi53405
MGIiMGI:1917912 Clic5

Phylogenomic databases

eggNOGiKOG1422 Eukaryota
ENOG410ZRK6 LUCA
GeneTreeiENSGT00550000074477
HOGENOMiHOG000231548
HOVERGENiHBG050994
InParanoidiQ8BXK9
KOiK05025
OMAiGAICKAG
OrthoDBiEOG091G0IHT
PhylomeDBiQ8BXK9
TreeFamiTF315438

Miscellaneous databases

ChiTaRSiClic5 mouse
PROiPR:Q8BXK9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023959 Expressed in 155 organ(s), highest expression level in lung
CleanExiMM_CLIC5
GenevisibleiQ8BXK9 MM

Family and domain databases

InterProiView protein in InterPro
IPR002946 CLIC
IPR030264 CLIC-5
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR036249 Thioredoxin-like_sf
PANTHERiPTHR43920:SF6 PTHR43920:SF6, 1 hit
PfamiView protein in Pfam
PF13417 GST_N_3, 1 hit
PRINTSiPR01263 INTCLCHANNEL
SFLDiSFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
TIGRFAMsiTIGR00862 O-ClC, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCLIC5_MOUSE
AccessioniPrimary (citable) accession number: Q8BXK9
Secondary accession number(s): Q3U0H8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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