UniProtKB - Q8BX65 (MDFIC_MOUSE)
MyoD family inhibitor domain-containing protein
Mdfic
Functioni
GO - Molecular functioni
- cyclin binding Source: UniProtKB
- Tat protein binding Source: UniProtKB
- transcription factor binding Source: UniProtKB
GO - Biological processi
- activation of JUN kinase activity Source: MGI
- negative regulation of protein import into nucleus Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of viral transcription Source: UniProtKB
- regulation of Wnt signaling pathway Source: MGI
Keywordsi
Molecular function | Activator, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: MyoD family inhibitor domain-containing proteinAlternative name(s): I-mfa domain-containing protein Kidney cell line-derived transcript 1 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104611, Mdfic |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000280223 | 1 – 247 | MyoD family inhibitor domain-containing proteinAdd BLAST | 247 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 129 | PhosphoserineBy similarity | 1 | |
Modified residuei | 141 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q8BX65 |
PaxDbi | Q8BX65 |
PRIDEi | Q8BX65 |
ProteomicsDBi | 252758 [Q8BX65-1] 252759 [Q8BX65-2] |
PTM databases
iPTMneti | Q8BX65 |
PhosphoSitePlusi | Q8BX65 |
SwissPalmi | Q8BX65 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000041390, Expressed in female gonad and 264 other tissues |
ExpressionAtlasi | Q8BX65, baseline and differential |
Genevisiblei | Q8BX65, MM |
Interactioni
Subunit structurei
Interacts with HAND1; leading to sequester HAND1 into the nucleolus and prevent its activity (PubMed:17891141).
Interacts with ZIC2 (PubMed:15207726, PubMed:15465018). The C-terminus interacts with AXIN1, the histidine-rich region of CCNT1/cyclin-T and weakly with LEF1.
Interacts with CCNT2 (By similarity).
By similarity3 PublicationsGO - Molecular functioni
- cyclin binding Source: UniProtKB
- Tat protein binding Source: UniProtKB
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
IntActi | Q8BX65, 1 interactor |
STRINGi | 10090.ENSMUSP00000099186 |
Miscellaneous databases
RNActi | Q8BX65, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 74 – 247 | MDFIAdd BLAST | 174 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 167 – 244 | Cys-richSequence analysisAdd BLAST | 78 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QSK8, Eukaryota |
GeneTreei | ENSGT00940000158685 |
HOGENOMi | CLU_067479_0_1_1 |
InParanoidi | Q8BX65 |
OrthoDBi | 1487193at2759 |
PhylomeDBi | Q8BX65 |
TreeFami | TF332113 |
Family and domain databases
InterProi | View protein in InterPro IPR026134, MDFI/MDFIC |
PANTHERi | PTHR15304, PTHR15304, 1 hit |
Pfami | View protein in Pfam PF15316, MDFI, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSCAGEALAP GPAEQQCPVE AGGGRLGSPA HEACNEDNTE KDKRPATSGH
60 70 80 90 100
TRCGLMRDQS IWPNPSAGEL VRTQPERLPQ LQTSAQEPGK EETGKIKNGG
110 120 130 140 150
HTRMSNGNGI PHGAKHVSVE NHKISAPVSQ KMHRKIQSSL SVNNDISKKS
160 170 180 190 200
KVNAVFSPKA ASSPEDCCVH CILACLFCEF LTLCNIVLGQ ASCGICTSEA
210 220 230 240
CCCCCGDEMG DDCSCPCDMD CGIMDACCES SDCLEICMEC CGICFPS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WRH9 | A0A087WRH9_MOUSE | MyoD family inhibitor domain-contai... | Mdfic | 329 | Annotation score: | ||
A0A087WPB2 | A0A087WPB2_MOUSE | MyoD family inhibitor domain-contai... | Mdfic | 200 | Annotation score: | ||
D6REI0 | D6REI0_MOUSE | MyoD family inhibitor domain-contai... | Mdfic | 109 | Annotation score: | ||
A0A087WRW6 | A0A087WRW6_MOUSE | MyoD family inhibitor domain-contai... | Mdfic | 112 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_052336 | 32 – 72 | Missing in isoform 2. 1 PublicationAdd BLAST | 41 | |
Alternative sequenceiVSP_052337 | 73 | T → A in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK048821 mRNA Translation: BAC33469.1 AK080178 mRNA Translation: BAC37840.1 Different initiation. AK165016 mRNA Translation: BAE38001.1 AK172522 mRNA Translation: BAE43047.1 Different initiation. BC067006 mRNA Translation: AAH67006.1 Different initiation. |
CCDSi | CCDS19919.1 [Q8BX65-1] |
RefSeqi | NP_780297.3, NM_175088.5 [Q8BX65-1] |
Genome annotation databases
Ensembli | ENSMUST00000101663; ENSMUSP00000099186; ENSMUSG00000041390 [Q8BX65-1] ENSMUST00000120512; ENSMUSP00000113050; ENSMUSG00000041390 [Q8BX65-1] ENSMUST00000189359; ENSMUSP00000140208; ENSMUSG00000041390 [Q8BX65-1] |
GeneIDi | 16543 |
KEGGi | mmu:16543 |
UCSCi | uc009azc.2, mouse [Q8BX65-1] uc012eif.1, mouse [Q8BX65-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK048821 mRNA Translation: BAC33469.1 AK080178 mRNA Translation: BAC37840.1 Different initiation. AK165016 mRNA Translation: BAE38001.1 AK172522 mRNA Translation: BAE43047.1 Different initiation. BC067006 mRNA Translation: AAH67006.1 Different initiation. |
CCDSi | CCDS19919.1 [Q8BX65-1] |
RefSeqi | NP_780297.3, NM_175088.5 [Q8BX65-1] |
3D structure databases
SMRi | Q8BX65 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q8BX65, 1 interactor |
STRINGi | 10090.ENSMUSP00000099186 |
PTM databases
iPTMneti | Q8BX65 |
PhosphoSitePlusi | Q8BX65 |
SwissPalmi | Q8BX65 |
Proteomic databases
jPOSTi | Q8BX65 |
PaxDbi | Q8BX65 |
PRIDEi | Q8BX65 |
ProteomicsDBi | 252758 [Q8BX65-1] 252759 [Q8BX65-2] |
Protocols and materials databases
Antibodypediai | 31567, 152 antibodies |
DNASUi | 16543 |
Genome annotation databases
Ensembli | ENSMUST00000101663; ENSMUSP00000099186; ENSMUSG00000041390 [Q8BX65-1] ENSMUST00000120512; ENSMUSP00000113050; ENSMUSG00000041390 [Q8BX65-1] ENSMUST00000189359; ENSMUSP00000140208; ENSMUSG00000041390 [Q8BX65-1] |
GeneIDi | 16543 |
KEGGi | mmu:16543 |
UCSCi | uc009azc.2, mouse [Q8BX65-1] uc012eif.1, mouse [Q8BX65-2] |
Organism-specific databases
CTDi | 29969 |
MGIi | MGI:104611, Mdfic |
Phylogenomic databases
eggNOGi | ENOG502QSK8, Eukaryota |
GeneTreei | ENSGT00940000158685 |
HOGENOMi | CLU_067479_0_1_1 |
InParanoidi | Q8BX65 |
OrthoDBi | 1487193at2759 |
PhylomeDBi | Q8BX65 |
TreeFami | TF332113 |
Miscellaneous databases
BioGRID-ORCSi | 16543, 0 hits in 52 CRISPR screens |
ChiTaRSi | Mdfic, mouse |
PROi | PR:Q8BX65 |
RNActi | Q8BX65, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000041390, Expressed in female gonad and 264 other tissues |
ExpressionAtlasi | Q8BX65, baseline and differential |
Genevisiblei | Q8BX65, MM |
Family and domain databases
InterProi | View protein in InterPro IPR026134, MDFI/MDFIC |
PANTHERi | PTHR15304, PTHR15304, 1 hit |
Pfami | View protein in Pfam PF15316, MDFI, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MDFIC_MOUSE | |
Accessioni | Q8BX65Primary (citable) accession number: Q8BX65 Secondary accession number(s): Q3T9H3, Q6NXM1, Q8BJS3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 2007 |
Last sequence update: | March 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families