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Protein

Serine/threonine-protein phosphatase PGAM5, mitochondrial

Gene

Pgam5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression (By similarity). Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

GO - Biological processi

  • dephosphorylation Source: UniProtKB
  • necroptotic process Source: UniProtKB
  • negative regulation of cold-induced thermogenesis Source: YuBioLab
  • positive regulation of GTPase activity Source: UniProtKB

Keywordsi

Molecular functionHydrolase
Biological processNecrosis

Enzyme and pathway databases

ReactomeiR-MMU-8934903 Receptor Mediated Mitophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PGAM5, mitochondrial (EC:3.1.3.16)
Alternative name(s):
Phosphoglycerate mutase family member 5
Gene namesi
Name:Pgam5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1919792 Pgam5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei7 – 29HelicalSequence analysisAdd BLAST23

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2331071

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002887831 – 288Serine/threonine-protein phosphatase PGAM5, mitochondrialAdd BLAST288

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei79PhosphoserineBy similarity1
Modified residuei86PhosphoserineBy similarity1
Modified residuei115N6-acetyllysineBy similarity1
Modified residuei143N6-acetyllysineBy similarity1
Modified residuei190N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BX10
PaxDbiQ8BX10
PeptideAtlasiQ8BX10
PRIDEiQ8BX10

PTM databases

iPTMnetiQ8BX10
PhosphoSitePlusiQ8BX10

Expressioni

Gene expression databases

BgeeiENSMUSG00000029500 Expressed in 273 organ(s), highest expression level in dorsal pancreas
CleanExiMM_PGAM5
ExpressionAtlasiQ8BX10 baseline and differential
GenevisibleiQ8BX10 MM

Interactioni

Subunit structurei

Dimer. Forms a ternary complex with NFE2L2 and KEAP1. Interacts with BCL2L1 and MAP3K5 (By similarity). Upon TNF-induced necrosis, forms in complex with RIPK1, RIPK3 and MLKL; the formation of this complex leads to PGAM5 phosphorylation (By similarity). Interacts with DNM1L; this interaction leads to DNM1L dephosphorylation and activation and eventually to mitochondria fragmentation (By similarity).By similarity

Protein-protein interaction databases

BioGridi215426, 4 interactors
DIPiDIP-32064N
IntActiQ8BX10, 5 interactors
STRINGi10090.ENSMUSP00000108124

Structurei

3D structure databases

ProteinModelPortaliQ8BX10
SMRiQ8BX10
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 81Interaction with KEAP1By similarity6

Domaini

The N-terminal 35 amino acids, including the potential transmembrane alpha-helix, function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4609 Eukaryota
ENOG410XXCQ LUCA
GeneTreeiENSGT00390000004796
HOGENOMiHOG000261217
HOVERGENiHBG105576
InParanoidiQ8BX10
KOiK15637
OMAiAVQYHED
OrthoDBiEOG091G0DEJ
PhylomeDBiQ8BX10
TreeFamiTF314977

Family and domain databases

CDDicd07067 HP_PGM_like, 1 hit
Gene3Di3.40.50.1240, 1 hit
InterProiView protein in InterPro
IPR013078 His_Pase_superF_clade-1
IPR029033 His_PPase_superfam
PfamiView protein in Pfam
PF00300 His_Phos_1, 1 hit
SMARTiView protein in SMART
SM00855 PGAM, 1 hit
SUPFAMiSSF53254 SSF53254, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BX10-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAFRQALQLA ACGLAGGSAA VLFSAVAVGK PRGGGDADTR ATEPPAWTGA
60 70 80 90 100
RAGRGVWDTN WDRREPLSLI NLKKRNVESG EDELTSRLDH YKAKATRHIF
110 120 130 140 150
LIRHSQYHVD GSLEKDRTLT PLGREQAELT GLRLASLGLK FNKIVHSSMT
160 170 180 190 200
RAVETTDIIS KHLPGVSRVS TDLLREGAPI EPDPPVSHWK PEAVQYYEDG
210 220 230 240 250
ARIEAAFRNY IHRADARQEE DSYEIFICHA NVIRYIVCRA LQFPPEGWLR
260 270 280
LSLNNGSITH LVIRPNGRVA LRTLGDTGFM PPDKITRS
Length:288
Mass (Da):31,994
Last modified:March 1, 2003 - v1
Checksum:iB704CDF4E640F888
GO
Isoform 2 (identifier: Q8BX10-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     195-195: Missing.

Show »
Length:287
Mass (Da):31,866
Checksum:iEF709F19D24DBBD6
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JG95A0A0G2JG95_MOUSE
Serine/threonine-protein phosphatas...
Pgam5
179Annotation score:

Sequence cautioni

The sequence BAB28067 differs from that shown. Reason: Frameshift at positions 52 and 66.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46A → V in BAE26984 (PubMed:16141072).Curated1
Sequence conflicti102I → M in BAC28763 (PubMed:16141072).Curated1
Sequence conflicti144I → V in BAB28067 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025762195Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012159 mRNA Translation: BAB28067.1 Frameshift.
AK034588 mRNA Translation: BAC28763.1
AK049246 mRNA Translation: BAC33634.1
AK146216 mRNA Translation: BAE26984.1
AK169643 mRNA Translation: BAE41272.1
BC052179 mRNA Translation: AAH52179.1
BC138924 mRNA Translation: AAI38925.1
BC138925 mRNA Translation: AAI38926.1
CCDSiCCDS19523.1 [Q8BX10-2]
CCDS51606.1 [Q8BX10-1]
RefSeqiNP_001157010.1, NM_001163538.1 [Q8BX10-1]
NP_082549.2, NM_028273.3 [Q8BX10-2]
UniGeneiMm.61682

Genome annotation databases

EnsembliENSMUST00000059229; ENSMUSP00000057760; ENSMUSG00000029500 [Q8BX10-2]
ENSMUST00000112505; ENSMUSP00000108124; ENSMUSG00000029500 [Q8BX10-1]
GeneIDi72542
KEGGimmu:72542
UCSCiuc008yqh.2 mouse [Q8BX10-2]
uc008yqi.2 mouse [Q8BX10-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012159 mRNA Translation: BAB28067.1 Frameshift.
AK034588 mRNA Translation: BAC28763.1
AK049246 mRNA Translation: BAC33634.1
AK146216 mRNA Translation: BAE26984.1
AK169643 mRNA Translation: BAE41272.1
BC052179 mRNA Translation: AAH52179.1
BC138924 mRNA Translation: AAI38925.1
BC138925 mRNA Translation: AAI38926.1
CCDSiCCDS19523.1 [Q8BX10-2]
CCDS51606.1 [Q8BX10-1]
RefSeqiNP_001157010.1, NM_001163538.1 [Q8BX10-1]
NP_082549.2, NM_028273.3 [Q8BX10-2]
UniGeneiMm.61682

3D structure databases

ProteinModelPortaliQ8BX10
SMRiQ8BX10
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215426, 4 interactors
DIPiDIP-32064N
IntActiQ8BX10, 5 interactors
STRINGi10090.ENSMUSP00000108124

Chemistry databases

ChEMBLiCHEMBL2331071

PTM databases

iPTMnetiQ8BX10
PhosphoSitePlusiQ8BX10

Proteomic databases

EPDiQ8BX10
PaxDbiQ8BX10
PeptideAtlasiQ8BX10
PRIDEiQ8BX10

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059229; ENSMUSP00000057760; ENSMUSG00000029500 [Q8BX10-2]
ENSMUST00000112505; ENSMUSP00000108124; ENSMUSG00000029500 [Q8BX10-1]
GeneIDi72542
KEGGimmu:72542
UCSCiuc008yqh.2 mouse [Q8BX10-2]
uc008yqi.2 mouse [Q8BX10-1]

Organism-specific databases

CTDi192111
MGIiMGI:1919792 Pgam5

Phylogenomic databases

eggNOGiKOG4609 Eukaryota
ENOG410XXCQ LUCA
GeneTreeiENSGT00390000004796
HOGENOMiHOG000261217
HOVERGENiHBG105576
InParanoidiQ8BX10
KOiK15637
OMAiAVQYHED
OrthoDBiEOG091G0DEJ
PhylomeDBiQ8BX10
TreeFamiTF314977

Enzyme and pathway databases

ReactomeiR-MMU-8934903 Receptor Mediated Mitophagy

Miscellaneous databases

ChiTaRSiPgam5 mouse
PROiPR:Q8BX10
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029500 Expressed in 273 organ(s), highest expression level in dorsal pancreas
CleanExiMM_PGAM5
ExpressionAtlasiQ8BX10 baseline and differential
GenevisibleiQ8BX10 MM

Family and domain databases

CDDicd07067 HP_PGM_like, 1 hit
Gene3Di3.40.50.1240, 1 hit
InterProiView protein in InterPro
IPR013078 His_Pase_superF_clade-1
IPR029033 His_PPase_superfam
PfamiView protein in Pfam
PF00300 His_Phos_1, 1 hit
SMARTiView protein in SMART
SM00855 PGAM, 1 hit
SUPFAMiSSF53254 SSF53254, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPGAM5_MOUSE
AccessioniPrimary (citable) accession number: Q8BX10
Secondary accession number(s): B2RSM6
, Q3UK19, Q80VY8, Q8BM78, Q9CZU2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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