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Protein

Retinoblastoma-binding protein 5

Gene

Rbbp5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid. Does not affect ES cell self-renewal.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-201722 Formation of the beta-catenin:TCF transactivating complex
R-MMU-3214841 PKMTs methylate histone lysines
R-MMU-3769402 Deactivation of the beta-catenin transactivating complex
R-MMU-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-MMU-8951664 Neddylation

Names & Taxonomyi

Protein namesi
Recommended name:
Retinoblastoma-binding protein 5
Short name:
RBBP-5
Gene namesi
Name:Rbbp5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1918367 Rbbp5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003909991 – 538Retinoblastoma-binding protein 5By similarityAdd BLAST538

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki129Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei252Phosphothreonine; by CDK1By similarity1
Modified residuei350PhosphoserineBy similarity1
Modified residuei388PhosphoserineBy similarity1
Modified residuei389PhosphoserineBy similarity1
Modified residuei497Phosphoserine; by CDK1By similarity1
Modified residuei525PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BX09
PaxDbiQ8BX09
PeptideAtlasiQ8BX09
PRIDEiQ8BX09

PTM databases

iPTMnetiQ8BX09
PhosphoSitePlusiQ8BX09

Expressioni

Gene expression databases

BgeeiENSMUSG00000026439 Expressed in 246 organ(s), highest expression level in pes
ExpressionAtlasiQ8BX09 baseline and differential
GenevisibleiQ8BX09 MM

Interactioni

Subunit structurei

Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, CFC1 and DPY30 (By similarity). Interacts with WDR82 and SETD1A (By similarity). Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity (By similarity). Core component of several methyltransferase-containing complexes including MLL1/MLL, MLL2/3 (also named ASCOM complex) and MLL4/WBP7. Each complex is at least composed of ASH2L, RBBP5, DPY30, WDR5, one or more specific histone methyltransferases (KMT2A/MLL1, KMT2D/MLL2, KMT2C/MLL3 and KMT2B/MLL4), and the facultative components PAGR1, BAP18, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, MYST1/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9, TEX10 and alpha- and beta-tubulin. Interacts with ZNF335.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ash2lQ91X2010EBI-1556543,EBI-1556554

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229436, 12 interactors
DIPiDIP-61809N
IntActiQ8BX09, 14 interactors
MINTiQ8BX09
STRINGi10090.ENSMUSP00000027700

Structurei

3D structure databases

ProteinModelPortaliQ8BX09
SMRiQ8BX09
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8BX09

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati22 – 63WD 1Add BLAST42
Repeati64 – 103WD 2Add BLAST40
Repeati148 – 188WD 3Add BLAST41
Repeati196 – 235WD 4Add BLAST40
Repeati249 – 291WD 5Add BLAST43
Repeati293 – 331WD 6Add BLAST39

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1273 Eukaryota
ENOG410XTA2 LUCA
GeneTreeiENSGT00530000064100
HOGENOMiHOG000204856
HOVERGENiHBG054324
InParanoidiQ8BX09
KOiK14961
OMAiFRMPILF
OrthoDBiEOG091G038T
PhylomeDBiQ8BX09
TreeFamiTF313289

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR037850 RBBP5/Swd1
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
PANTHERiPTHR44040 PTHR44040, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 5 hits
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q8BX09-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNLELLESFG QNYPEEADGT LDCISMALTC TFNRWGTLLA VGCNDGRIVI
60 70 80 90 100
WDFLTRGIAK IISAHIHPVC SLCWSRDGHK LVSASTDNIV SQWDVLSGDC
110 120 130 140 150
DQRFRFPSPI LKVQYHPRDQ NKVLVCPMKS APVMLTLSDS KHVVLPVDDD
160 170 180 190 200
SDLNVVASFD RRGEYIYTGN AKGKILVLKT DSQDLVASFR VTTGTSNTTA
210 220 230 240 250
IKSIEFARKG SCFLINTADR IIRVYDGREI LTCGRDGEPE PMQKLQDLVN
260 270 280 290 300
RTPWKKCCFS GDGEYIVAGS ARQHALYIWE KSIGNLVKIL HGTRGELLLD
310 320 330 340 350
VAWHPVRPII ASISSGVVSI WAQNQVENWS AFAPDFKELD ENVEYEERES
360 370 380 390 400
EFDIEDEDKS EPEQTGADAA EDEEVDVTSV DPIAAFCSSD EELEDSKALL
410 420 430 440 450
YLPIAPEVED PEENPYGPPP DAVPSSLMDE GASSEKKRQS SADGSQPPKK
460 470 480 490 500
KPKTTNIELQ GVPNDEVHPL LGVKGDGKSK KKQAGRPKGS KGKEKDSPFK
510 520 530
PKLYKGDRGL PLEGSTKGKV QAELSQSLAA GGAISELL
Length:538
Mass (Da):59,098
Last modified:January 19, 2010 - v2
Checksum:i7D4E9FD5B38A7EB0
GO
Isoform 2 (identifier: Q8BX09-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-127: Missing.
     492-529: Missing.

Note: No experimental confirmation available.
Show »
Length:373
Mass (Da):40,764
Checksum:iD5BD12F61C597B19
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WRV7A0A087WRV7_MOUSE
Retinoblastoma-binding protein 5
Rbbp5
149Annotation score:
A0A0R4J2B6A0A0R4J2B6_MOUSE
Retinoblastoma-binding protein 5
Rbbp5
537Annotation score:
A0A087WQU2A0A087WQU2_MOUSE
Retinoblastoma-binding protein 5
Rbbp5
107Annotation score:
A0A087WST2A0A087WST2_MOUSE
Retinoblastoma-binding protein 5
Rbbp5
410Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti243Q → K in BAC33635 (PubMed:16141072).Curated1
Sequence conflicti529Missing in BAE42295 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0386711 – 127Missing in isoform 2. 1 PublicationAdd BLAST127
Alternative sequenceiVSP_038672492 – 529Missing in isoform 2. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049247 mRNA Translation: BAC33635.1
AK154782 mRNA Translation: BAE32825.1
AK171177 mRNA Translation: BAE42295.1
CH466520 Genomic DNA Translation: EDL39676.1
BC057632 mRNA Translation: AAH57632.1
BC060186 mRNA Translation: AAH60186.2
CCDSiCCDS15286.1 [Q8BX09-1]
RefSeqiNP_766105.2, NM_172517.2 [Q8BX09-1]
UniGeneiMm.132868

Genome annotation databases

EnsembliENSMUST00000027700; ENSMUSP00000027700; ENSMUSG00000026439 [Q8BX09-2]
ENSMUST00000190997; ENSMUSP00000141003; ENSMUSG00000026439 [Q8BX09-1]
GeneIDi213464
KEGGimmu:213464
UCSCiuc007cow.2 mouse [Q8BX09-1]
uc007coy.2 mouse [Q8BX09-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRBBP5_MOUSE
AccessioniPrimary (citable) accession number: Q8BX09
Secondary accession number(s): Q3TBL4
, Q3U3G1, Q6PAP0, Q6PFC2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: January 19, 2010
Last modified: September 12, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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