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Protein

Calcium release-activated calcium channel protein 1

Gene

Orai1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.By similarity

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • calcium ion import Source: MGI
  • mammary gland epithelium development Source: MGI
  • positive regulation of calcium ion transport Source: UniProtKB
  • regulation of calcium ion transport Source: MGI
  • store-operated calcium entry Source: MGI

Keywordsi

Molecular functionCalcium channel, Calmodulin-binding, Ion channel
Biological processAdaptive immunity, Calcium transport, Immunity, Ion transport, Transport
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium release-activated calcium channel protein 1
Alternative name(s):
Protein orai-1
Transmembrane protein 142A
Gene namesi
Name:Orai1
Synonyms:Cracm1, Tmem142a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1925542 Orai1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 89CytoplasmicBy similarityAdd BLAST89
Transmembranei90 – 107HelicalSequence analysisAdd BLAST18
Topological domaini108 – 121ExtracellularSequence analysisAdd BLAST14
Transmembranei122 – 142HelicalSequence analysisAdd BLAST21
Topological domaini143 – 175CytoplasmicSequence analysisAdd BLAST33
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Topological domaini197 – 237ExtracellularSequence analysisAdd BLAST41
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
Topological domaini259 – 304CytoplasmicBy similarityAdd BLAST46

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002343821 – 304Calcium release-activated calcium channel protein 1Add BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi225N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei298PhosphothreonineBy similarity1

Post-translational modificationi

N-glycosylated.By similarity
Ubiquitinated.By similarity
Cys-197 is oxidated, leading to inactivate channel activity.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BWG9
MaxQBiQ8BWG9
PaxDbiQ8BWG9
PRIDEiQ8BWG9

PTM databases

iPTMnetiQ8BWG9
PhosphoSitePlusiQ8BWG9
SwissPalmiQ8BWG9

Expressioni

Gene expression databases

BgeeiENSMUSG00000049686 Expressed in 220 organ(s), highest expression level in vastus lateralis
ExpressionAtlasiQ8BWG9 baseline and differential
GenevisibleiQ8BWG9 MM

Interactioni

Subunit structurei

Interacts with STIM1 and STIM2; this regulates channel activity. Interacts with CALM; this may displace STIM1 and STIM2 and might thereby modulate channel activity. Interacts with CRACR2A/EFCAB4B; the interaction is direct and takes place in absence of Ca2+. Forms a complex with CRACR2A/EFCAB4B and STIM1 at low concentration of Ca2+, the complex dissociates at elevated Ca2+ concentrations. Interacts with SLC35G1. Interacts with UBQLN1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi224640, 1 interactor
STRINGi10090.ENSMUSP00000058169

Structurei

3D structure databases

ProteinModelPortaliQ8BWG9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni72 – 92Interaction with STIM1By similarityAdd BLAST21
Regioni275 – 295Interaction with STIM1By similarityAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi43 – 50Poly-Pro8

Sequence similaritiesi

Belongs to the Orai family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4298 Eukaryota
ENOG4110DSB LUCA
GeneTreeiENSGT00390000015354
HOGENOMiHOG000246925
HOVERGENiHBG081343
InParanoidiQ8BWG9
KOiK16056
OMAiQLDHRGD
OrthoDBiEOG091G0E4P
PhylomeDBiQ8BWG9
TreeFamiTF313576

Family and domain databases

InterProiView protein in InterPro
IPR012446 CRAC_channel
PANTHERiPTHR31501 PTHR31501, 1 hit
PfamiView protein in Pfam
PF07856 Orai-1, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BWG9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHPEPAPPPS HSNPELPVSG GSSTSGSRRS RRRSGDGEPS GAPPLPPPPP
60 70 80 90 100
AVSYPDWIGQ SYSEVMSLNE HSMQALSWRK LYLSRAKLKA SSRTSALLSG
110 120 130 140 150
FAMVAMVEVQ LDTDHDYPPG LLIVFSACTT VLVAVHLFAL MISTCILPNI
160 170 180 190 200
EAVSNVHNLN SVKESPHERM HRHIELAWAF STVIGTLLFL AEVVLLCWVK
210 220 230 240 250
FLPLKRQAGQ PSPTKPPAES VIVANHSDSS GITPGEAAAI ASTAIMVPCG
260 270 280 290 300
LVFIVFAVHF YRSLVSHKTD RQFQELNELA EFARLQDQLD HRGDHSLTPG

THYA
Length:304
Mass (Da):33,062
Last modified:March 1, 2003 - v1
Checksum:i9743395B63D2E41F
GO

Sequence cautioni

The sequence AAH23149 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16L → F in AAH23149 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK052573 mRNA Translation: BAC35045.1
AK085755 mRNA Translation: BAC39533.1
BC023149 mRNA Translation: AAH23149.1 Different initiation.
CCDSiCCDS39261.1
RefSeqiNP_780632.1, NM_175423.3
UniGeneiMm.218582

Genome annotation databases

EnsembliENSMUST00000051016; ENSMUSP00000058169; ENSMUSG00000049686
ENSMUST00000121652; ENSMUSP00000113097; ENSMUSG00000049686
GeneIDi109305
KEGGimmu:109305
UCSCiuc008zmy.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK052573 mRNA Translation: BAC35045.1
AK085755 mRNA Translation: BAC39533.1
BC023149 mRNA Translation: AAH23149.1 Different initiation.
CCDSiCCDS39261.1
RefSeqiNP_780632.1, NM_175423.3
UniGeneiMm.218582

3D structure databases

ProteinModelPortaliQ8BWG9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi224640, 1 interactor
STRINGi10090.ENSMUSP00000058169

PTM databases

iPTMnetiQ8BWG9
PhosphoSitePlusiQ8BWG9
SwissPalmiQ8BWG9

Proteomic databases

EPDiQ8BWG9
MaxQBiQ8BWG9
PaxDbiQ8BWG9
PRIDEiQ8BWG9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051016; ENSMUSP00000058169; ENSMUSG00000049686
ENSMUST00000121652; ENSMUSP00000113097; ENSMUSG00000049686
GeneIDi109305
KEGGimmu:109305
UCSCiuc008zmy.1 mouse

Organism-specific databases

CTDi84876
MGIiMGI:1925542 Orai1

Phylogenomic databases

eggNOGiKOG4298 Eukaryota
ENOG4110DSB LUCA
GeneTreeiENSGT00390000015354
HOGENOMiHOG000246925
HOVERGENiHBG081343
InParanoidiQ8BWG9
KOiK16056
OMAiQLDHRGD
OrthoDBiEOG091G0E4P
PhylomeDBiQ8BWG9
TreeFamiTF313576

Miscellaneous databases

ChiTaRSiOrai1 mouse
PROiPR:Q8BWG9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049686 Expressed in 220 organ(s), highest expression level in vastus lateralis
ExpressionAtlasiQ8BWG9 baseline and differential
GenevisibleiQ8BWG9 MM

Family and domain databases

InterProiView protein in InterPro
IPR012446 CRAC_channel
PANTHERiPTHR31501 PTHR31501, 1 hit
PfamiView protein in Pfam
PF07856 Orai-1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCRCM1_MOUSE
AccessioniPrimary (citable) accession number: Q8BWG9
Secondary accession number(s): Q8BUD4, Q8R586
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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