UniProtKB - Q8BW41 (PMGT2_MOUSE)
Protein
Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2
Gene
Pomgnt2
Organism
Mus musculus (Mouse)
Status
Functioni
O-linked mannose beta-1,4-N-acetylglucosaminyltransferase that transfers UDP-N-acetyl-D-glucosamine to the 4-position of the mannose to generate N-acetyl-D-glucosamine-beta-1,4-O-D-mannosylprotein (By similarity). Involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed:24256719).By similarity1 Publication
Catalytic activityi
- 3-O-(α-D-mannosyl)-L-threonyl-[protein] + UDP-N-acetyl-α-D-glucosamine = 3-O-(N-acetyl-β-D-glucosaminyl-(1→4)-α-D-mannosyl)-L-threonyl-[protein] + H+ + UDPBy similarityEC:2.4.1.312By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- acetylglucosaminyltransferase activity Source: UniProtKB
- protein O-GlcNAc transferase activity Source: MGI
- transferase activity, transferring glycosyl groups Source: GO_Central
GO - Biological processi
- neuron migration Source: UniProtKB
- protein O-linked glycosylation Source: UniProtKB
- protein O-linked mannosylation Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
Reactomei | R-MMU-5173105, O-linked glycosylation |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT61, Glycosyltransferase Family 61 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 (EC:2.4.1.312By similarity)Short name: POMGnT2 Alternative name(s): Extracellular O-linked N-acetylglucosamine transferase-like Glycosyltransferase-like domain-containing protein 2 |
Gene namesi | Name:Pomgnt2 Synonyms:Ago61, Eogtl, Gtdc2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2143424, Pomgnt2 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass type II membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 4 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 5 – 25 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 26 – 580 | LumenalSequence analysisAdd BLAST | 555 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
Newborn are slightly smaller and die within the first day of birth due to abnormal basal lamina formation and neuronal migration defects. Defects are due to a lack of laminin-binding glycans.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000249015 | 1 – 605 | Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2Add BLAST | 605 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 99 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 276 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q8BW41 |
PeptideAtlasi | Q8BW41 |
PRIDEi | Q8BW41 |
ProteomicsDBi | 289457 [Q8BW41-1] 289458 [Q8BW41-2] |
PTM databases
GlyConnecti | 2638, 4 N-Linked glycans (2 sites) |
GlyGeni | Q8BW41, 3 sites |
iPTMneti | Q8BW41 |
PhosphoSitePlusi | Q8BW41 |
SwissPalmi | Q8BW41 |
Expressioni
Tissue specificityi
Mainly expressed in the central nervous system.1 Publication
Developmental stagei
Expressed during brain development. At 14.5 dpc, expressed throughout the cortical plate and the developing brain, as well as throughout the eye, including the lens and cornea. Cortical expression is already detected at 12.5 dpc, peaks between 14.5 and 16.5 dpc and is reduced at birth.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000066235, Expressed in facial nucleus and 253 other tissues |
ExpressionAtlasi | Q8BW41, baseline and differential |
Genevisiblei | Q8BW41, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 229636, 1 interactor |
STRINGi | 10090.ENSMUSP00000095868 |
Miscellaneous databases
RNActi | Q8BW41, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 488 – 584 | Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST | 97 |
Sequence similaritiesi
Belongs to the glycosyltransferase 61 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4698, Eukaryota |
GeneTreei | ENSGT00940000160695 |
HOGENOMi | CLU_020169_0_0_1 |
InParanoidi | Q8BW41 |
OMAi | EVWIQEQ |
OrthoDBi | 820160at2759 |
PhylomeDBi | Q8BW41 |
TreeFami | TF332712 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR003961, FN3_dom IPR036116, FN3_sf IPR007657, Glycosyltransferase_61 IPR013783, Ig-like_fold |
PANTHERi | PTHR20961, PTHR20961, 1 hit |
Pfami | View protein in Pfam PF04577, DUF563, 1 hit |
SUPFAMi | SSF49265, SSF49265, 1 hit |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8BW41-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MHLSAVFNAL LVSVLAAVLW KHVRLREHAA TLEEELALGQ QSLDPVLGLK
60 70 80 90 100
IDYPKALQIL MEGGTHMVCT GRTHTDRICR FKWLCYSNEA EEFIFFHGNS
110 120 130 140 150
SVMLPNLGSR RFQPALLDLS TVEDHNAQYF NFVELPAAAL RFMPKPVFVP
160 170 180 190 200
DVALIANRFN PDNLMHVFHD DLLPLFYTLR QFPGLAQEAR LFFMEGWGEG
210 220 230 240 250
AHFDLYKLLS PKQPLLRAQL KTLGRLLCFS HAFVGLSKVT TWYQYGFVQP
260 270 280 290 300
QGPKANILVS GNEIRQFTRF MTERLNVSHA GAPLGEEYIL VFSRTQNRLI
310 320 330 340 350
LNEAELLLEL AQEFQMKTVT VSLEDHTFAD VVRLVSNASM LVSMHGAQLV
360 370 380 390 400
TALFLPRGAT VVELFPYAVN PDHYTPYKTL ATLPGMDLQY VAWRNMIREN
410 420 430 440 450
TVTHPERPWD QGGITHLDRA EQARILQSRE VPRHLCCRNP EWLFRIYQDT
460 470 480 490 500
RVDIPSLMQS IRRVVKGRPG PRRQRWAISL YPGKVREARC QASVQGATEA
510 520 530 540 550
RLSVSWQIPW NLKYLKVREV KYEVWLQEQG ENTYVPYMLT LQNHTFTENI
560 570 580 590 600
KPFTTYLVWV RCIFNRSLLG PFCRCAGVQH VASRPWPGLW RAPLAGSAFP
GPQFC
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1L1SV90 | A0A1L1SV90_MOUSE | Protein O-linked-mannose beta-1,4-N... | Pomgnt2 | 154 | Annotation score: | ||
A0A1L1SUZ1 | A0A1L1SUZ1_MOUSE | Protein O-linked-mannose beta-1,4-N... | Pomgnt2 | 41 | Annotation score: | ||
A0A1L1SSQ2 | A0A1L1SSQ2_MOUSE | Protein O-linked-mannose beta-1,4-N... | Pomgnt2 | 22 | Annotation score: |
Sequence cautioni
The sequence BAC31348 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 567 | S → T in AAH30931 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020354 | 573 – 580 | CRCAGVQH → ADVLVCST in isoform 2. 2 Publications | 8 | |
Alternative sequenceiVSP_020355 | 581 – 605 | Missing in isoform 2. 2 PublicationsAdd BLAST | 25 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK042747 mRNA Translation: BAC31348.1 Sequence problems. AK054403 mRNA Translation: BAC35765.1 AK133802 mRNA Translation: BAE21851.1 BC025056 mRNA Translation: AAH25056.1 BC030931 mRNA Translation: AAH30931.1 |
CCDSi | CCDS23643.1 [Q8BW41-2] |
RefSeqi | NP_001276487.1, NM_001289558.1 [Q8BW41-2] NP_001276488.1, NM_001289559.1 [Q8BW41-2] NP_001276489.1, NM_001289560.1 [Q8BW41-2] NP_705768.4, NM_153540.4 [Q8BW41-2] XP_011241257.1, XM_011242955.1 [Q8BW41-2] |
Genome annotation databases
Ensembli | ENSMUST00000084743; ENSMUSP00000095868; ENSMUSG00000066235 [Q8BW41-2] ENSMUST00000216669; ENSMUSP00000149077; ENSMUSG00000066235 [Q8BW41-2] ENSMUST00000217610; ENSMUSP00000149753; ENSMUSG00000066235 [Q8BW41-2] |
GeneIDi | 215494 |
KEGGi | mmu:215494 |
UCSCi | uc009sek.2, mouse [Q8BW41-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK042747 mRNA Translation: BAC31348.1 Sequence problems. AK054403 mRNA Translation: BAC35765.1 AK133802 mRNA Translation: BAE21851.1 BC025056 mRNA Translation: AAH25056.1 BC030931 mRNA Translation: AAH30931.1 |
CCDSi | CCDS23643.1 [Q8BW41-2] |
RefSeqi | NP_001276487.1, NM_001289558.1 [Q8BW41-2] NP_001276488.1, NM_001289559.1 [Q8BW41-2] NP_001276489.1, NM_001289560.1 [Q8BW41-2] NP_705768.4, NM_153540.4 [Q8BW41-2] XP_011241257.1, XM_011242955.1 [Q8BW41-2] |
3D structure databases
SMRi | Q8BW41 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 229636, 1 interactor |
STRINGi | 10090.ENSMUSP00000095868 |
Protein family/group databases
CAZyi | GT61, Glycosyltransferase Family 61 |
PTM databases
GlyConnecti | 2638, 4 N-Linked glycans (2 sites) |
GlyGeni | Q8BW41, 3 sites |
iPTMneti | Q8BW41 |
PhosphoSitePlusi | Q8BW41 |
SwissPalmi | Q8BW41 |
Proteomic databases
PaxDbi | Q8BW41 |
PeptideAtlasi | Q8BW41 |
PRIDEi | Q8BW41 |
ProteomicsDBi | 289457 [Q8BW41-1] 289458 [Q8BW41-2] |
Protocols and materials databases
Antibodypediai | 29340, 155 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000084743; ENSMUSP00000095868; ENSMUSG00000066235 [Q8BW41-2] ENSMUST00000216669; ENSMUSP00000149077; ENSMUSG00000066235 [Q8BW41-2] ENSMUST00000217610; ENSMUSP00000149753; ENSMUSG00000066235 [Q8BW41-2] |
GeneIDi | 215494 |
KEGGi | mmu:215494 |
UCSCi | uc009sek.2, mouse [Q8BW41-2] |
Organism-specific databases
CTDi | 84892 |
MGIi | MGI:2143424, Pomgnt2 |
Phylogenomic databases
eggNOGi | KOG4698, Eukaryota |
GeneTreei | ENSGT00940000160695 |
HOGENOMi | CLU_020169_0_0_1 |
InParanoidi | Q8BW41 |
OMAi | EVWIQEQ |
OrthoDBi | 820160at2759 |
PhylomeDBi | Q8BW41 |
TreeFami | TF332712 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Reactomei | R-MMU-5173105, O-linked glycosylation |
Miscellaneous databases
BioGRID-ORCSi | 215494, 1 hit in 53 CRISPR screens |
PROi | PR:Q8BW41 |
RNActi | Q8BW41, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000066235, Expressed in facial nucleus and 253 other tissues |
ExpressionAtlasi | Q8BW41, baseline and differential |
Genevisiblei | Q8BW41, MM |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR003961, FN3_dom IPR036116, FN3_sf IPR007657, Glycosyltransferase_61 IPR013783, Ig-like_fold |
PANTHERi | PTHR20961, PTHR20961, 1 hit |
Pfami | View protein in Pfam PF04577, DUF563, 1 hit |
SUPFAMi | SSF49265, SSF49265, 1 hit |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PMGT2_MOUSE | |
Accessioni | Q8BW41Primary (citable) accession number: Q8BW41 Secondary accession number(s): Q58F17, Q8BXZ9, Q8K0M5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families