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Protein

Calcium-responsive transactivator

Gene

Ss18l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Chromatin regulator
Biological processTranscription, Transcription regulation
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-responsive transactivator
Alternative name(s):
SS18-like protein 1
Gene namesi
Name:Ss18l1
Synonyms:Crest, Kiaa0693
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2444061 Ss18l1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are smaller than littermates and show coordination defects. There is increased mortality in beginning at about P14, and about 80% die by P28. Less than 20% survive to adulthood, and they are infertile.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003913461 – 402Calcium-responsive transactivatorAdd BLAST402

Proteomic databases

MaxQBiQ8BW22
PaxDbiQ8BW22
PeptideAtlasiQ8BW22
PRIDEiQ8BW22

PTM databases

iPTMnetiQ8BW22
PhosphoSitePlusiQ8BW22

Expressioni

Gene expression databases

BgeeiENSMUSG00000039086 Expressed in 224 organ(s), highest expression level in forebrain
GenevisibleiQ8BW22 MM

Interactioni

Subunit structurei

Homodimer. Dimerization may be necessary for its function in neuronal dendritic development. Interacts (via C-terminus) with CREBBP (via N-terminus), EP300 and SMARCA4/BRG1. Interacts with the nBAF complex. Association with CREBBP facilitates transcription while the association with SMARCA4/BRG1 suppresses CREST-mediated transcription in resting neurons (By similarity).By similarity

Protein-protein interaction databases

BioGridi234646, 2 interactors
CORUMiQ8BW22
IntActiQ8BW22, 1 interactor
MINTiQ8BW22
STRINGi10090.ENSMUSP00000041288

Structurei

3D structure databases

ProteinModelPortaliQ8BW22
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 148N-terminal auto-inhibitory domain; necessary for interaction with SMARCA4/BRG1By similarityAdd BLAST148
Regioni149 – 238Methionine-rich intra-molecular domainBy similarityAdd BLAST90
Regioni252 – 323MFD domainBy similarityAdd BLAST72
Regioni340 – 402Necessary for nuclear localizationBy similarityAdd BLAST63
Regioni393 – 402Necessary for interaction with CREBBP and for the recruitment of CREBBP to the nuclear bodiesBy similarity10

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi50 – 53SH2-bindingSequence analysis4
Motifi359 – 362SH2-bindingSequence analysis4
Motifi377 – 385SH3-bindingSequence analysis9
Motifi397 – 400SH2-bindingSequence analysis4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi197 – 402Gln-richAdd BLAST206

Domaini

The MFD (multi-functional domain) domain is involved in transcription transactivation, nuclear body targeting and dimerization.By similarity

Sequence similaritiesi

Belongs to the SS18 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3227 Eukaryota
ENOG41128HA LUCA
GeneTreeiENSGT00500000044808
HOGENOMiHOG000048717
HOVERGENiHBG003892
InParanoidiQ8BW22
OMAiHYNSAQG
OrthoDBiEOG091G0O10
PhylomeDBiQ8BW22
TreeFamiTF330999

Family and domain databases

InterProiView protein in InterPro
IPR007726 SS18_fam
PfamiView protein in Pfam
PF05030 SSXT, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BW22-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVAFASARP RGKGEVTQQT IQKMLDENHH LIQCILDYQS KGKTAECTQY
60 70 80 90 100
QQILHRNLVY LATIADSNQN MQSLLPAPPT QNMNLGPGAL SQSGSSQGLH
110 120 130 140 150
PQGSLSDTVS TGLPPASLMQ GQIGNGPNHV SMQQTAQSTL PTTSMSLSGS
160 170 180 190 200
GHGTGPGYSH SGPTSQSVPM QGQGAISNYV SRTNINMQSN PVSMMHQQAA
210 220 230 240 250
TSHYNSAQGG SQHYQGQAPI AMMGQGGQGG SMMGQRPMAP YRPSQQGSSQ
260 270 280 290 300
QYLGQEEYYS EQYSHSQGSA EPMSQQYYPD GHGDYAYQQS SYTEQSYDRS
310 320 330 340 350
FEDPTQHYYE GGNSQYSQQQ AGYQQGTAQQ QTYSQQQYPN QQSYPGQQQG
360 370 380 390 400
YGPAQGAPSQ YSSYQQGQGQ QYGSYRTSQT GPSAQQQRPY GYEQGQYGNY

QQ
Length:402
Mass (Da):43,729
Last modified:March 1, 2003 - v1
Checksum:i9C309B9E7446CCAA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti130V → L in BAC30089 (PubMed:16141072).Curated1
Sequence conflicti159S → I in AAH54730 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173004 mRNA Translation: BAD32282.1
AK038669 mRNA Translation: BAC30089.1
AK054560 mRNA Translation: BAC35824.1
AK133944 mRNA Translation: BAE21942.1
AL663067 Genomic DNA No translation available.
BC053087 mRNA Translation: AAH53087.1
BC054730 mRNA Translation: AAH54730.1
CCDSiCCDS17167.1
RefSeqiNP_848865.4, NM_178750.5
UniGeneiMm.63068

Genome annotation databases

EnsembliENSMUST00000041126; ENSMUSP00000041288; ENSMUSG00000039086
GeneIDi269397
KEGGimmu:269397
UCSCiuc008oic.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173004 mRNA Translation: BAD32282.1
AK038669 mRNA Translation: BAC30089.1
AK054560 mRNA Translation: BAC35824.1
AK133944 mRNA Translation: BAE21942.1
AL663067 Genomic DNA No translation available.
BC053087 mRNA Translation: AAH53087.1
BC054730 mRNA Translation: AAH54730.1
CCDSiCCDS17167.1
RefSeqiNP_848865.4, NM_178750.5
UniGeneiMm.63068

3D structure databases

ProteinModelPortaliQ8BW22
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234646, 2 interactors
CORUMiQ8BW22
IntActiQ8BW22, 1 interactor
MINTiQ8BW22
STRINGi10090.ENSMUSP00000041288

PTM databases

iPTMnetiQ8BW22
PhosphoSitePlusiQ8BW22

Proteomic databases

MaxQBiQ8BW22
PaxDbiQ8BW22
PeptideAtlasiQ8BW22
PRIDEiQ8BW22

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041126; ENSMUSP00000041288; ENSMUSG00000039086
GeneIDi269397
KEGGimmu:269397
UCSCiuc008oic.2 mouse

Organism-specific databases

CTDi26039
MGIiMGI:2444061 Ss18l1
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3227 Eukaryota
ENOG41128HA LUCA
GeneTreeiENSGT00500000044808
HOGENOMiHOG000048717
HOVERGENiHBG003892
InParanoidiQ8BW22
OMAiHYNSAQG
OrthoDBiEOG091G0O10
PhylomeDBiQ8BW22
TreeFamiTF330999

Miscellaneous databases

PROiPR:Q8BW22
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039086 Expressed in 224 organ(s), highest expression level in forebrain
GenevisibleiQ8BW22 MM

Family and domain databases

InterProiView protein in InterPro
IPR007726 SS18_fam
PfamiView protein in Pfam
PF05030 SSXT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCREST_MOUSE
AccessioniPrimary (citable) accession number: Q8BW22
Secondary accession number(s): Q6A014, Q7TQF3, Q8BYQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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