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Protein

Gephyrin

Gene

Gphn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.By similarity

Catalytic activityi

ATP + molybdopterin = diphosphate + adenylyl-molybdopterin.By similarity
Adenylyl-molybdopterin + molybdate = molybdenum cofactor + AMP.By similarity

Cofactori

Mg2+By similarity

Activity regulationi

Inhibited by copper and tungsten.By similarity

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMultifunctional enzyme, Transferase
Biological processMolybdenum cofactor biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Molybdenum, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-947581 Molybdenum cofactor biosynthesis
UniPathwayi
UPA00344

Names & Taxonomyi

Protein namesi
Recommended name:
Gephyrin
Including the following 2 domains:
Molybdopterin adenylyltransferase (EC:2.7.7.75By similarity)
Short name:
MPT adenylyltransferase
Alternative name(s):
Domain G
Molybdopterin molybdenumtransferase (EC:2.10.1.1By similarity)
Short name:
MPT Mo-transferase
Alternative name(s):
Domain E
Gene namesi
Name:Gphn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:109602 Gphn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002690391 – 769GephyrinAdd BLAST769

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188PhosphoserineCombined sources1
Modified residuei194PhosphoserineCombined sources1
Modified residuei198PhosphothreonineBy similarity1
Modified residuei200PhosphoserineCombined sources1
Modified residuei262PhosphoserineCombined sources1
Modified residuei265PhosphothreonineCombined sources1
Modified residuei266PhosphothreonineCombined sources1
Modified residuei268PhosphoserineCombined sources1
Modified residuei270PhosphoserineCombined sources1
Modified residuei338PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BUV3
MaxQBiQ8BUV3
PeptideAtlasiQ8BUV3
PRIDEiQ8BUV3

PTM databases

iPTMnetiQ8BUV3
PhosphoSitePlusiQ8BUV3
SwissPalmiQ8BUV3

Expressioni

Gene expression databases

BgeeiENSMUSG00000047454 Expressed in 296 organ(s), highest expression level in central nervous system
CleanExiMM_GPHN
ExpressionAtlasiQ8BUV3 baseline and differential
GenevisibleiQ8BUV3 MM

Interactioni

Subunit structurei

Homotrimer, homodimer and homooligomer (By similarity). Interacts with SRGAP2 (via SH3 domain) (PubMed:22126966). Interacts with GLRB (By similarity). Interacts with GABARAP (By similarity). Interacts with GABRA3 (By similarity). GABRA3 and GLRB occupy overlapping binding sites (By similarity). Interacts with ARHGAP32; IQSEC3, INSYN1 and INSYN2 (PubMed:27609886).By similarity2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi234521, 5 interactors
ELMiQ8BUV3
IntActiQ8BUV3, 8 interactors
MINTiQ8BUV3

Structurei

3D structure databases

ProteinModelPortaliQ8BUV3
SMRiQ8BUV3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 153MPT Mo-transferaseAdd BLAST140
Regioni140 – 349Interaction with GABARAPBy similarityAdd BLAST210
Regioni327 – 769MPT adenylyltransferaseAdd BLAST443

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi187 – 192Poly-Pro6
Compositional biasi213 – 218Glu-rich (acidic)6

Sequence similaritiesi

In the N-terminal section; belongs to the MoaB/Mog family.Curated
In the C-terminal section; belongs to the MoeA family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000016577
HOGENOMiHOG000280651
HOVERGENiHBG005828
InParanoidiQ8BUV3
KOiK15376
OMAiHFGRVAM

Family and domain databases

CDDicd00887 MoeA, 1 hit
cd00886 MogA_MoaB, 1 hit
Gene3Di2.40.340.10, 1 hit
3.40.980.10, 2 hits
InterProiView protein in InterPro
IPR036425 MoaB/Mog-like_dom_sf
IPR001453 MoaB/Mog_dom
IPR008284 MoCF_biosynth_CS
IPR038987 MoeA-like
IPR005111 MoeA_C_domain_IV
IPR036688 MoeA_C_domain_IV_sf
IPR005110 MoeA_linker/N
IPR036135 MoeA_linker/N_sf
PANTHERiPTHR10192 PTHR10192, 1 hit
PfamiView protein in Pfam
PF00994 MoCF_biosynth, 2 hits
PF03454 MoeA_C, 1 hit
PF03453 MoeA_N, 1 hit
SMARTiView protein in SMART
SM00852 MoCF_biosynth, 2 hits
SUPFAMiSSF53218 SSF53218, 2 hits
SSF63867 SSF63867, 1 hit
SSF63882 SSF63882, 1 hit
TIGRFAMsiTIGR00177 molyb_syn, 2 hits
PROSITEiView protein in PROSITE
PS01078 MOCF_BIOSYNTHESIS_1, 1 hit
PS01079 MOCF_BIOSYNTHESIS_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8BUV3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATEGMILTN HDHQIRVGVL TVSDSCFRNL AEDRSGINLK DLVQDPSLLG
60 70 80 90 100
GTISAYKIVP DEIEEIKETL IDWCDEKELN LILTTGGTGF APRDVTPEAT
110 120 130 140 150
KEVIEREAPG MALAMLMGSL NVTPLGMLSR PVCGIRGKTL IINLPGSKKG
160 170 180 190 200
SQECFQFILP ALPHAIDLLR DAIVKVKEVH DELEDLPSPP PPLSPPPTTS
210 220 230 240 250
PHKQTEDKGV QCEEEEEEKK DSGVASTEDS SSSHITAAAL AAKIPDSIIS
260 270 280 290 300
RGVQVLPRDT ASLSTTPSES PRAQATSRLS TASCPTPKQI RRPDESKGVA
310 320 330 340 350
SRVGSLKARL PSCSSTYSVS EVQSRCSSKE NILRASHSAV DITKVARRHR
360 370 380 390 400
MSPFPLTSMD KAFITVLEMT PVLGTEIINY RDGMGRVLAQ DVYAKDNLPP
410 420 430 440 450
FPASVKDGYA VRAADGPGDR FIIGESQAGE QPTQTVMPGQ VMRVTTGAPI
460 470 480 490 500
PCGADAVVQV EDTELIRESD DGTEELEVRI LVQARPGQDI RPIGHDIKRG
510 520 530 540 550
ECVLAKGTHM GPSEIGLLAT VGVTEVEVNK FPVVAVMSTG NELLNPEDDL
560 570 580 590 600
LPGKIRDSNR STLLATIQEH GYPTINLGIV GDNPDDLLNA LNEGISRADV
610 620 630 640 650
IITSGGVSMG EKDYLKQVLD IDLHAQIHFG RVFMKPGLPT TFATLDIDGV
660 670 680 690 700
RKIIFALPGN PVSAVVTCNL FVVPALRKMQ GILDPRPTII KARLSCDVKL
710 720 730 740 750
DPRPEYHRCI LTWHHQEPLP WAQSTGNQMS SRLMSMRSAN GLLMLPPKTE
760
QYVELHKGEV VDVMVIGRL
Length:769
Mass (Da):83,282
Last modified:July 27, 2011 - v2
Checksum:iD0EF325CC3CF87E9
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0JNY3A0JNY3_MOUSE
Gephyrin
Gphn
772Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti262S → T in BAC38476 (PubMed:16141072).Curated1
Sequence conflicti463T → S in BAC38476 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK082353 mRNA Translation: BAC38476.1
AC124346 Genomic DNA No translation available.
AC124572 Genomic DNA No translation available.
AC125057 Genomic DNA No translation available.
AC132097 Genomic DNA No translation available.
AC148324 Genomic DNA No translation available.
CCDSiCCDS26000.1
RefSeqiNP_766540.2, NM_172952.3
UniGeneiMm.341742
Mm.363753
Mm.453131

Genome annotation databases

EnsembliENSMUST00000052472; ENSMUSP00000054064; ENSMUSG00000047454
GeneIDi268566
KEGGimmu:268566
UCSCiuc007nzc.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK082353 mRNA Translation: BAC38476.1
AC124346 Genomic DNA No translation available.
AC124572 Genomic DNA No translation available.
AC125057 Genomic DNA No translation available.
AC132097 Genomic DNA No translation available.
AC148324 Genomic DNA No translation available.
CCDSiCCDS26000.1
RefSeqiNP_766540.2, NM_172952.3
UniGeneiMm.341742
Mm.363753
Mm.453131

3D structure databases

ProteinModelPortaliQ8BUV3
SMRiQ8BUV3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234521, 5 interactors
ELMiQ8BUV3
IntActiQ8BUV3, 8 interactors
MINTiQ8BUV3

PTM databases

iPTMnetiQ8BUV3
PhosphoSitePlusiQ8BUV3
SwissPalmiQ8BUV3

Proteomic databases

EPDiQ8BUV3
MaxQBiQ8BUV3
PeptideAtlasiQ8BUV3
PRIDEiQ8BUV3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052472; ENSMUSP00000054064; ENSMUSG00000047454
GeneIDi268566
KEGGimmu:268566
UCSCiuc007nzc.2 mouse

Organism-specific databases

CTDi10243
MGIiMGI:109602 Gphn

Phylogenomic databases

GeneTreeiENSGT00390000016577
HOGENOMiHOG000280651
HOVERGENiHBG005828
InParanoidiQ8BUV3
KOiK15376
OMAiHFGRVAM

Enzyme and pathway databases

UniPathwayi
UPA00344

ReactomeiR-MMU-947581 Molybdenum cofactor biosynthesis

Miscellaneous databases

ChiTaRSiGphn mouse
PROiPR:Q8BUV3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047454 Expressed in 296 organ(s), highest expression level in central nervous system
CleanExiMM_GPHN
ExpressionAtlasiQ8BUV3 baseline and differential
GenevisibleiQ8BUV3 MM

Family and domain databases

CDDicd00887 MoeA, 1 hit
cd00886 MogA_MoaB, 1 hit
Gene3Di2.40.340.10, 1 hit
3.40.980.10, 2 hits
InterProiView protein in InterPro
IPR036425 MoaB/Mog-like_dom_sf
IPR001453 MoaB/Mog_dom
IPR008284 MoCF_biosynth_CS
IPR038987 MoeA-like
IPR005111 MoeA_C_domain_IV
IPR036688 MoeA_C_domain_IV_sf
IPR005110 MoeA_linker/N
IPR036135 MoeA_linker/N_sf
PANTHERiPTHR10192 PTHR10192, 1 hit
PfamiView protein in Pfam
PF00994 MoCF_biosynth, 2 hits
PF03454 MoeA_C, 1 hit
PF03453 MoeA_N, 1 hit
SMARTiView protein in SMART
SM00852 MoCF_biosynth, 2 hits
SUPFAMiSSF53218 SSF53218, 2 hits
SSF63867 SSF63867, 1 hit
SSF63882 SSF63882, 1 hit
TIGRFAMsiTIGR00177 molyb_syn, 2 hits
PROSITEiView protein in PROSITE
PS01078 MOCF_BIOSYNTHESIS_1, 1 hit
PS01079 MOCF_BIOSYNTHESIS_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGEPH_MOUSE
AccessioniPrimary (citable) accession number: Q8BUV3
Secondary accession number(s): E9QKJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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