UniProtKB - Q8BU31 (RAP2C_MOUSE)
Protein
Ras-related protein Rap-2c
Gene
Rap2c
Organism
Mus musculus (Mouse)
Status
Functioni
Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May play a role in SRE-mediated gene transcription.1 Publication
Catalytic activityi
- EC:3.6.5.2By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 17 | GTPBy similarity | 8 | |
Nucleotide bindingi | 57 – 61 | GTPBy similarity | 5 | |
Nucleotide bindingi | 116 – 119 | GTPBy similarity | 4 |
GO - Molecular functioni
- GDP binding Source: MGI
- GTPase activity Source: GO_Central
- GTP binding Source: MGI
- transcription coactivator activity Source: MGI
GO - Biological processi
- establishment of endothelial intestinal barrier Source: MGI
- negative regulation of cell migration Source: MGI
- positive regulation of protein autophosphorylation Source: MGI
- Rap protein signal transduction Source: UniProtKB
- regulation of protein tyrosine kinase activity Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Rap2c |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919315, Rap2c |
Subcellular locationi
Endosome
- Recycling endosome membrane 1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication
Other locations
- Cytoplasm By similarity
Cytosol
- cytosol Source: MGI
Endosome
- recycling endosome Source: MGI
- recycling endosome membrane Source: UniProtKB
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- bicellular tight junction Source: MGI
- cell-cell contact zone Source: MGI
- cytoplasm Source: MGI
- membrane Source: MGI
Keywords - Cellular componenti
Cytoplasm, Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 176 | C → G: Loss of association with the recycling endosome membranes and loss of TNIK activation; when associated with C-177. 1 Publication | 1 | |
Mutagenesisi | 177 | C → G: Loss of association with the recycling endosome membranes and loss of TNIK activation; when associated with C-176. 1 Publication | 1 | |
Mutagenesisi | 180 | C → A: Loss of association with membranes. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000030223 | 1 – 180 | Ras-related protein Rap-2cAdd BLAST | 180 | |
PropeptideiPRO_0000030224 | 181 – 183 | Removed in mature form1 Publication | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 176 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 177 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 180 | Cysteine methyl ester1 Publication | 1 | |
Lipidationi | 180 | S-geranylgeranyl cysteine1 Publication | 1 |
Post-translational modificationi
Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK.1 Publication
Keywords - PTMi
Lipoprotein, Methylation, Palmitate, PrenylationProteomic databases
EPDi | Q8BU31 |
jPOSTi | Q8BU31 |
MaxQBi | Q8BU31 |
PaxDbi | Q8BU31 |
PeptideAtlasi | Q8BU31 |
PRIDEi | Q8BU31 |
PTM databases
iPTMneti | Q8BU31 |
PhosphoSitePlusi | Q8BU31 |
SwissPalmi | Q8BU31 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000050029, Expressed in embryonic post-anal tail and 288 other tissues |
Genevisiblei | Q8BU31, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 215127, 2 interactors |
STRINGi | 10090.ENSMUSP00000058391 |
Miscellaneous databases
RNActi | Q8BU31, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 32 – 40 | Effector regionCurated | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000157245 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | Q8BU31 |
OMAi | QQIYRIK |
OrthoDBi | 1353024at2759 |
PhylomeDBi | Q8BU31 |
TreeFami | TF313014 |
Family and domain databases
CDDi | cd04176, Rap2, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR041840, Rap2 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8BU31-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MREYKVVVLG SGGVGKSALT VQFVTGTFIE KYDPTIEDFY RKEIEVDSSP
60 70 80 90 100
SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI
110 120 130 140 150
VRVKRYEKVP LILVGNKVDL EPEREVMSSE GRALAQEWGC PFMETSAKSK
160 170 180
SMVDELFAEI VRQMNYSSLP EKQDQCCTTC VVQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 83 | S → N in AAH27363 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK087971 mRNA Translation: BAC40066.1 AK146057 mRNA Translation: BAE26866.1 AK146956 mRNA Translation: BAE27564.1 AK147015 mRNA Translation: BAE27609.1 BC027363 mRNA Translation: AAH27363.3 BC050056 mRNA Translation: AAH50056.2 BC064814 mRNA Translation: AAH64814.2 BC094890 mRNA Translation: AAH94890.1 |
CCDSi | CCDS30121.1 |
RefSeqi | NP_766001.1, NM_172413.2 XP_006541608.1, XM_006541545.2 XP_006541609.1, XM_006541546.2 |
Genome annotation databases
Ensembli | ENSMUST00000053593; ENSMUSP00000058391; ENSMUSG00000050029 |
GeneIDi | 72065 |
KEGGi | mmu:72065 |
UCSCi | uc009tds.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK087971 mRNA Translation: BAC40066.1 AK146057 mRNA Translation: BAE26866.1 AK146956 mRNA Translation: BAE27564.1 AK147015 mRNA Translation: BAE27609.1 BC027363 mRNA Translation: AAH27363.3 BC050056 mRNA Translation: AAH50056.2 BC064814 mRNA Translation: AAH64814.2 BC094890 mRNA Translation: AAH94890.1 |
CCDSi | CCDS30121.1 |
RefSeqi | NP_766001.1, NM_172413.2 XP_006541608.1, XM_006541545.2 XP_006541609.1, XM_006541546.2 |
3D structure databases
SMRi | Q8BU31 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 215127, 2 interactors |
STRINGi | 10090.ENSMUSP00000058391 |
PTM databases
iPTMneti | Q8BU31 |
PhosphoSitePlusi | Q8BU31 |
SwissPalmi | Q8BU31 |
Proteomic databases
EPDi | Q8BU31 |
jPOSTi | Q8BU31 |
MaxQBi | Q8BU31 |
PaxDbi | Q8BU31 |
PeptideAtlasi | Q8BU31 |
PRIDEi | Q8BU31 |
Protocols and materials databases
Antibodypediai | 56035, 122 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000053593; ENSMUSP00000058391; ENSMUSG00000050029 |
GeneIDi | 72065 |
KEGGi | mmu:72065 |
UCSCi | uc009tds.1, mouse |
Organism-specific databases
CTDi | 57826 |
MGIi | MGI:1919315, Rap2c |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000157245 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | Q8BU31 |
OMAi | QQIYRIK |
OrthoDBi | 1353024at2759 |
PhylomeDBi | Q8BU31 |
TreeFami | TF313014 |
Enzyme and pathway databases
Reactomei | R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 72065, 0 hits in 17 CRISPR screens |
ChiTaRSi | Rap2c, mouse |
PROi | PR:Q8BU31 |
RNActi | Q8BU31, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000050029, Expressed in embryonic post-anal tail and 288 other tissues |
Genevisiblei | Q8BU31, MM |
Family and domain databases
CDDi | cd04176, Rap2, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR041840, Rap2 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAP2C_MOUSE | |
Accessioni | Q8BU31Primary (citable) accession number: Q8BU31 Secondary accession number(s): Q504Q0 Q8R2P4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families