UniProtKB - Q8BU30 (SYIC_MOUSE)
Protein
Isoleucine--tRNA ligase, cytoplasmic
Gene
Iars1
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.1 Publication
Catalytic activityi
- EC:6.1.1.51 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 603 | ATPBy similarity | 1 |
GO - Molecular functioni
- aminoacyl-tRNA editing activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- GTPase binding Source: MGI
- isoleucine-tRNA ligase activity Source: CAFA
- tRNA binding Source: InterPro
GO - Biological processi
- isoleucyl-tRNA aminoacylation Source: CAFA
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Isoleucine--tRNA ligase, cytoplasmic (EC:6.1.1.51 Publication)Alternative name(s): Isoleucyl-tRNA synthetase Short name: IRS Short name: IleRS |
Gene namesi | Name:Iars1 Synonyms:Iars |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2145219, Iars |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB
Other locations
- aminoacyl-tRNA synthetase multienzyme complex Source: CAFA
- cytoplasm Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000098598 | 1 – 1262 | Isoleucine--tRNA ligase, cytoplasmicAdd BLAST | 1262 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 1049 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1058 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8BU30 |
jPOSTi | Q8BU30 |
MaxQBi | Q8BU30 |
PaxDbi | Q8BU30 |
PeptideAtlasi | Q8BU30 |
PRIDEi | Q8BU30 |
PTM databases
iPTMneti | Q8BU30 |
PhosphoSitePlusi | Q8BU30 |
SwissPalmi | Q8BU30 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000037851, Expressed in forelimb bud and 125 other tissues |
Genevisiblei | Q8BU30, MM |
Interactioni
Subunit structurei
Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18.
1 PublicationGO - Molecular functioni
- GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 222774, 8 interactors |
IntActi | Q8BU30, 2 interactors |
MINTi | Q8BU30 |
STRINGi | 10090.ENSMUSP00000132082 |
Miscellaneous databases
RNActi | Q8BU30, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 48 – 58 | 'HIGH' regionAdd BLAST | 11 | |
Motifi | 600 – 604 | 'KMSKS' region | 5 |
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | KOG0434, Eukaryota |
GeneTreei | ENSGT00550000074921 |
HOGENOMi | CLU_001493_1_1_1 |
InParanoidi | Q8BU30 |
OMAi | GYRVMPY |
OrthoDBi | 59501at2759 |
PhylomeDBi | Q8BU30 |
Family and domain databases
CDDi | cd07961, Anticodon_Ia_Ile_ABEc, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.90.740.10, 1 hit |
HAMAPi | MF_02003, Ile_tRNA_synth_type2, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033709, Anticodon_Ile_ABEc IPR002301, Ile-tRNA-ligase IPR023586, Ile-tRNA-ligase_type2 IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit |
PRINTSi | PR00984, TRNASYNTHILE |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00392, ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q8BU30-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVQQVPENIS FPAEEEKILE FWSKHNCFQE CLKQSKLRPK FTFYDGPPFA
60 70 80 90 100
TGLPHYGHIL AGTIKDIVTR YAHQSGFHVD RRFGWDCHGL PVEYEIDKTL
110 120 130 140 150
GIKGPEDVAK MGIAEYNKQC RAIVMRYSAE WKSTVTRLGR WIDFDNDYKT
160 170 180 190 200
LYPQFMESVW WVFKQLYDKG LVYRGVKVMP FSTACGTPLS NFESNQNYKD
210 220 230 240 250
VQDPSVFVTF PLEEDENTSL VAWTTTPWTL PSNLALCVNP EIQYVKIKDV
260 270 280 290 300
ARGKLFILTE ARLSALYKQE SDYEILERFP GASLKGKKYK PLFDYFIKCK
310 320 330 340 350
ENGAFTVLVD HYVKDEEGTG VVHQAPYFGA DDHRVCMDFN IIQKDSVPVC
360 370 380 390 400
PVDASGCFTE EVTHFVGQYV KDADKNIIRM LKEQGRLLAA GTFTHSYPFC
410 420 430 440 450
WRSDTPLIYK SVPSWFVRVE PMVDQLLKNN DLCYWVPEFV REKRFGNWLK
460 470 480 490 500
EARDWAISRN RYWGTPIPLW VSEDLEEVVC IGSVAELEEL SGTKISDLHR
510 520 530 540 550
ESIDHLTIPS RCGKAPLRRV SEVFDCWFES GSMPYAQVHY PFESKREFED
560 570 580 590 600
AFPADFIAEG IDQTRGWFYT LLVLATALFG QPPFKNVIVN GLILASDGQK
610 620 630 640 650
MSKRKKNYPD PVSIIDKYGA DALRLYLINS PVVRAENLRF KEEGVRDVLK
660 670 680 690 700
DVLLPWYNAY RFFIQNVFRL HKEEEVKFLY NEHTVRESPN ITDRWVLSFM
710 720 730 740 750
QSLLGFFETE MAAYRLYTVV PRLVKFVDIL TNWYVRMNRR RLKGESGVED
760 770 780 790 800
CVMALETLFS VLLSLCRLMA PYTPFLTELM YQNLKLLIDP ASLRDKDTLS
810 820 830 840 850
IHYLMLPRVR EELIDKKTEN AVSRMQSVIE LGRVIRDRKT IPIKYPLKEI
860 870 880 890 900
VVIHQDPEAL EDIRSLEKYI IEELNVRKVT LSTDKNKYGI RLRAEPDHMV
910 920 930 940 950
LGKRLKGAFK AVMMAIKRLS NEELERFQKS GSIVVEGHEL HEEDIRLMYT
960 970 980 990 1000
FDQATGGTAQ FEAHSDAQAL VLLDVTPDQS MVDEGMAREV INRIQKLRKK
1010 1020 1030 1040 1050
CNLVPTDEIT VYYNAKSEGR YLNNVIESHT DFIFATIKAP LKPYPVPTSD
1060 1070 1080 1090 1100
NILIQEQTQL KGSELEITLT KGSCVPGPAC AYVNLNICAN GTEQGGVLLL
1110 1120 1130 1140 1150
ENPKGDNQLN LVKLKTVVTS VFGVKNAKLS VFHGETEIQN QTDLLSLSGR
1160 1170 1180 1190 1200
TLCVTAGASP SPISSPSTLL CQYLNLQLLN AEPQECLTGT VGTLLLENPL
1210 1220 1230 1240 1250
GQNGLTHQGL VHEAAKVFGL RSRRLRLFLN ETQTQEITED IPMKTLNMKT
1260
VYVSVLPTTA DG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q866 | E9Q866_MOUSE | Isoleucine-tRNA synthetase | Iars | 92 | Annotation score: | ||
F6Q6R1 | F6Q6R1_MOUSE | Isoleucine-tRNA synthetase | Iars | 96 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1033 | I → V in BAC40081 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK087992 mRNA Translation: BAC40081.1 AK133856 mRNA Translation: BAE21890.1 CH466546 Genomic DNA Translation: EDL41088.1 BC067029 mRNA Translation: AAH67029.1 |
CCDSi | CCDS36659.1 |
RefSeqi | NP_742012.2, NM_172015.3 XP_006516889.1, XM_006516826.2 |
Genome annotation databases
Ensembli | ENSMUST00000047363; ENSMUSP00000048096; ENSMUSG00000037851 ENSMUST00000164260; ENSMUSP00000126806; ENSMUSG00000037851 ENSMUST00000165316; ENSMUSP00000132082; ENSMUSG00000037851 |
GeneIDi | 105148 |
KEGGi | mmu:105148 |
UCSCi | uc007qjv.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK087992 mRNA Translation: BAC40081.1 AK133856 mRNA Translation: BAE21890.1 CH466546 Genomic DNA Translation: EDL41088.1 BC067029 mRNA Translation: AAH67029.1 |
CCDSi | CCDS36659.1 |
RefSeqi | NP_742012.2, NM_172015.3 XP_006516889.1, XM_006516826.2 |
3D structure databases
SMRi | Q8BU30 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 222774, 8 interactors |
IntActi | Q8BU30, 2 interactors |
MINTi | Q8BU30 |
STRINGi | 10090.ENSMUSP00000132082 |
PTM databases
iPTMneti | Q8BU30 |
PhosphoSitePlusi | Q8BU30 |
SwissPalmi | Q8BU30 |
Proteomic databases
EPDi | Q8BU30 |
jPOSTi | Q8BU30 |
MaxQBi | Q8BU30 |
PaxDbi | Q8BU30 |
PeptideAtlasi | Q8BU30 |
PRIDEi | Q8BU30 |
Protocols and materials databases
Antibodypediai | 28131, 129 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000047363; ENSMUSP00000048096; ENSMUSG00000037851 ENSMUST00000164260; ENSMUSP00000126806; ENSMUSG00000037851 ENSMUST00000165316; ENSMUSP00000132082; ENSMUSG00000037851 |
GeneIDi | 105148 |
KEGGi | mmu:105148 |
UCSCi | uc007qjv.2, mouse |
Organism-specific databases
CTDi | 105148 |
MGIi | MGI:2145219, Iars |
Phylogenomic databases
eggNOGi | KOG0434, Eukaryota |
GeneTreei | ENSGT00550000074921 |
HOGENOMi | CLU_001493_1_1_1 |
InParanoidi | Q8BU30 |
OMAi | GYRVMPY |
OrthoDBi | 59501at2759 |
PhylomeDBi | Q8BU30 |
Miscellaneous databases
BioGRID-ORCSi | 105148, 9 hits in 18 CRISPR screens |
ChiTaRSi | Iars, mouse |
PROi | PR:Q8BU30 |
RNActi | Q8BU30, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037851, Expressed in forelimb bud and 125 other tissues |
Genevisiblei | Q8BU30, MM |
Family and domain databases
CDDi | cd07961, Anticodon_Ia_Ile_ABEc, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.90.740.10, 1 hit |
HAMAPi | MF_02003, Ile_tRNA_synth_type2, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033709, Anticodon_Ile_ABEc IPR002301, Ile-tRNA-ligase IPR023586, Ile-tRNA-ligase_type2 IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit |
PRINTSi | PR00984, TRNASYNTHILE |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00392, ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYIC_MOUSE | |
Accessioni | Q8BU30Primary (citable) accession number: Q8BU30 Secondary accession number(s): Q6NXK4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 137 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries