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Protein

Cleavage and polyadenylation specificity factor subunit 7

Gene

Cpsf7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. CPSF7 activates directly the mRNA 3'-processing machinery. Binds to pA signals in RNA substrates.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing

Enzyme and pathway databases

ReactomeiR-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72187 mRNA 3'-end processing
R-MMU-77595 Processing of Intronless Pre-mRNAs

Names & Taxonomyi

Protein namesi
Recommended name:
Cleavage and polyadenylation specificity factor subunit 7Imported
Gene namesi
Name:Cpsf7Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1917826 Cpsf7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000815281 – 471Cleavage and polyadenylation specificity factor subunit 7Add BLAST471

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei203PhosphothreonineCombined sources1
Modified residuei205PhosphoserineCombined sources1
Cross-linki354Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei413PhosphoserineBy similarity1
Modified residuei423PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated.By similarity
Asymmetrically dimethylated on arginine residues by PRMT1.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8BTV2
PeptideAtlasiQ8BTV2
PRIDEiQ8BTV2

PTM databases

iPTMnetiQ8BTV2
PhosphoSitePlusiQ8BTV2

Miscellaneous databases

PMAP-CutDBiQ8BTV2

Expressioni

Gene expression databases

BgeeiENSMUSG00000034820 Expressed in 296 organ(s), highest expression level in mesenteric lymph node
CleanExiMM_5730453I16RIK
GenevisibleiQ8BTV2 MM

Interactioni

Subunit structurei

Component of the cleavage factor Im (CFIm) complex which is an heterotetramer composed of two subunits of NUDT21/CPSF5 and two subunits of CPSF6 or CPSF7 or an heterodimer of CPSF6 and CPSF7. The cleavage factor Im (CFIm) complex associates with the CPSF and CSTF complexes to promote the assembly of the core mRNA 3'-processing machinery. Interacts with NUDT21/CPSF5. Interacts (via Arg/Ser-rich domain) with FIP1L1 (preferentially via unphosphorylated form and Arg/Glu/Asp-rich region); this interaction mediates, at least in part, the interaction between the CFIm and CPSF complexes and may be inhibited by CPSF7 hyper-phosphorylation.By similarity

Protein-protein interaction databases

IntActiQ8BTV2, 1 interactor
MINTiQ8BTV2
STRINGi10090.ENSMUSP00000038958

Structurei

3D structure databases

ProteinModelPortaliQ8BTV2
SMRiQ8BTV2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 162RRMPROSITE-ProRule annotationAdd BLAST81

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni418 – 469Arg/Ser-rich domainBy similarityAdd BLAST52

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi51 – 54Poly-Pro4
Compositional biasi218 – 329Pro-richAdd BLAST112
Compositional biasi418 – 469Arg-richAdd BLAST52

Domaini

Contains an Arg/Ser-rich domain composed of arginine-serine dipeptide repeats within the C-terminal region that is necessary and sufficient for activating mRNA 3'-processing.By similarity

Sequence similaritiesi

Belongs to the RRM CPSF6/7 family.Curated

Phylogenomic databases

eggNOGiKOG4849 Eukaryota
ENOG4111NBM LUCA
GeneTreeiENSGT00730000110905
HOGENOMiHOG000111137
HOVERGENiHBG056699
InParanoidiQ8BTV2
KOiK14398
OMAiKASTPYN
OrthoDBiEOG091G0CVC
PhylomeDBiQ8BTV2
TreeFamiTF316430

Family and domain databases

CDDicd12644 RRM_CFIm59, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR034772 CPSF6/7
IPR034770 CPSF7
IPR034773 CPSF7_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PANTHERiPTHR23204 PTHR23204, 1 hit
PTHR23204:SF2 PTHR23204:SF2, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q8BTV2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSEGVDLIDI YADEEFNQDS EFNNTDQIDL YDDVLTAASQ PSDDRSSSTE
60 70 80 90 100
PPPPVRQEPA PKPNNKTPAI LYTYSGLRSR RAAVYVGSFS WWTTDQQLIQ
110 120 130 140 150
VIRSIGVYDV VELKFAENRA NGQSKGYAEV VVASENSVHK LLELLPGKVL
160 170 180 190 200
NGEKVDVRPA TRQNLSQFEA QARKRECVRV PRGGIPPRAH SRDSSDSADG
210 220 230 240 250
RATPSENLVP SSARVDKPPS VLPYFNRPPS ALPLMGLPPP PIPPPPPLSS
260 270 280 290 300
SFGVPPPPPG IHYQHLMPPP PRLPPHLAVP PPGAIPPALH LNPAFFPPPN
310 320 330 340 350
ATVGPPPDTY MKASTPYNHH GSRDSGPPPS TVSEAEFEEI MKRNRAISSS
360 370 380 390 400
AISKAVSGAS AGDYSDAIET LLTAIAVIKQ SRVANDERCR VLISSLKDCL
410 420 430 440 450
HGIEAKSYSV GASGSSSRKR HRSRERSPSR SRESSRRHRD LLHNEDRHDD
460 470
YFQERNREHE RHRDRERDRH H
Length:471
Mass (Da):52,011
Last modified:February 7, 2006 - v2
Checksum:i3ACC0F1FF7224109
GO
Isoform 2 (identifier: Q8BTV2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     176-184: Missing.

Show »
Length:462
Mass (Da):51,057
Checksum:i42D511ECC74E228E
GO
Isoform 3 (identifier: Q8BTV2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-234: Missing.

Show »
Length:237
Mass (Da):26,192
Checksum:iBA60549810D0C8FD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti262H → Q in BAC40447 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0171951 – 234Missing in isoform 3. 1 PublicationAdd BLAST234
Alternative sequenceiVSP_017196176 – 184Missing in isoform 2. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053375 mRNA Translation: BAC35368.1
AK165330 mRNA Translation: BAE38138.1
AK088603 mRNA Translation: BAC40447.1
BC038812 mRNA Translation: AAH38812.1
CCDSiCCDS29579.1 [Q8BTV2-1]
RefSeqiNP_001157744.1, NM_001164272.1 [Q8BTV2-2]
NP_758506.3, NM_172302.3 [Q8BTV2-1]
XP_006527179.1, XM_006527116.3 [Q8BTV2-1]
XP_006527186.1, XM_006527123.2
UniGeneiMm.490260

Genome annotation databases

EnsembliENSMUST00000038379; ENSMUSP00000038958; ENSMUSG00000034820 [Q8BTV2-1]
GeneIDi269061
KEGGimmu:269061
UCSCiuc008gpw.2 mouse [Q8BTV2-1]
uc008gqa.2 mouse [Q8BTV2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053375 mRNA Translation: BAC35368.1
AK165330 mRNA Translation: BAE38138.1
AK088603 mRNA Translation: BAC40447.1
BC038812 mRNA Translation: AAH38812.1
CCDSiCCDS29579.1 [Q8BTV2-1]
RefSeqiNP_001157744.1, NM_001164272.1 [Q8BTV2-2]
NP_758506.3, NM_172302.3 [Q8BTV2-1]
XP_006527179.1, XM_006527116.3 [Q8BTV2-1]
XP_006527186.1, XM_006527123.2
UniGeneiMm.490260

3D structure databases

ProteinModelPortaliQ8BTV2
SMRiQ8BTV2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BTV2, 1 interactor
MINTiQ8BTV2
STRINGi10090.ENSMUSP00000038958

PTM databases

iPTMnetiQ8BTV2
PhosphoSitePlusiQ8BTV2

Proteomic databases

PaxDbiQ8BTV2
PeptideAtlasiQ8BTV2
PRIDEiQ8BTV2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038379; ENSMUSP00000038958; ENSMUSG00000034820 [Q8BTV2-1]
GeneIDi269061
KEGGimmu:269061
UCSCiuc008gpw.2 mouse [Q8BTV2-1]
uc008gqa.2 mouse [Q8BTV2-2]

Organism-specific databases

CTDi79869
MGIiMGI:1917826 Cpsf7

Phylogenomic databases

eggNOGiKOG4849 Eukaryota
ENOG4111NBM LUCA
GeneTreeiENSGT00730000110905
HOGENOMiHOG000111137
HOVERGENiHBG056699
InParanoidiQ8BTV2
KOiK14398
OMAiKASTPYN
OrthoDBiEOG091G0CVC
PhylomeDBiQ8BTV2
TreeFamiTF316430

Enzyme and pathway databases

ReactomeiR-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72187 mRNA 3'-end processing
R-MMU-77595 Processing of Intronless Pre-mRNAs

Miscellaneous databases

PMAP-CutDBiQ8BTV2
PROiPR:Q8BTV2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034820 Expressed in 296 organ(s), highest expression level in mesenteric lymph node
CleanExiMM_5730453I16RIK
GenevisibleiQ8BTV2 MM

Family and domain databases

CDDicd12644 RRM_CFIm59, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR034772 CPSF6/7
IPR034770 CPSF7
IPR034773 CPSF7_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PANTHERiPTHR23204 PTHR23204, 1 hit
PTHR23204:SF2 PTHR23204:SF2, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCPSF7_MOUSE
AccessioniPrimary (citable) accession number: Q8BTV2
Secondary accession number(s): Q3TNF1, Q8BKE7, Q8CFS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: November 7, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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