UniProtKB - Q8BTQ0 (PCGF3_MOUSE)
Polycomb group RING finger protein 3
PcgF3
Functioni
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:28596365).
Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (By similarity).
Plays a redundant role with PCGF5 as part of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females (PubMed:28596365).
By similarity1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 17 – 56 | RING-typePROSITE-ProRule annotationAdd BLAST | 40 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- histone H2A-K119 monoubiquitination Source: UniProtKB
- inactivation of X chromosome by genomic imprinting Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Polycomb group RING finger protein 3Alternative name(s): RING finger protein 3A |
Gene namesi | Name:PcgF3 Synonyms:Rnf3, Rnf3a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1916837, Pcgf3 |
VEuPathDBi | HostDB:ENSMUSG00000033623 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
- nucleoplasm 1 Publication
Note: Recruited by the non-coding RNA Xist to specific nuclear foci that probably correspond to the inactivated X chromosome.1 Publication
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
- PcG protein complex Source: UniProtKB
- PRC1 complex Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000277865 | 1 – 241 | Polycomb group RING finger protein 3Add BLAST | 241 |
Proteomic databases
MaxQBi | Q8BTQ0 |
PaxDbi | Q8BTQ0 |
PRIDEi | Q8BTQ0 |
ProteomicsDBi | 287889 [Q8BTQ0-1] 287890 [Q8BTQ0-2] |
PTM databases
PhosphoSitePlusi | Q8BTQ0 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000033623, Expressed in dorsal root ganglion and 271 other tissues |
Genevisiblei | Q8BTQ0, MM |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 213553, 1 interactor |
IntActi | Q8BTQ0, 1 interactor |
STRINGi | 10090.ENSMUSP00000041790 |
Miscellaneous databases
RNActi | Q8BTQ0, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 115 – 148 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 131 – 241 | Interaction with BCORL1By similarityAdd BLAST | 111 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 17 – 56 | RING-typePROSITE-ProRule annotationAdd BLAST | 40 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2660, Eukaryota |
GeneTreei | ENSGT00940000158395 |
HOGENOMi | CLU_046427_4_3_1 |
InParanoidi | Q8BTQ0 |
OMAi | ILCNNEL |
OrthoDBi | 1203951at2759 |
PhylomeDBi | Q8BTQ0 |
TreeFami | TF324206 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR032443, RAWUL IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF16207, RAWUL, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLTRKIKLWD INAHITCRLC SGYLIDATTV TECLHTFCRS CLVKYLEENN
60 70 80 90 100
TCPTCRIVIH QSHPLQYIGH DRTMQDIVYK LVPGLQEAEM RKQREFYHKL
110 120 130 140 150
GMEVPGDIKG EACSAKQHLD PRNGETKADD NSNKETAEEK QEEDNDYHRS
160 170 180 190 200
DEQVSICLEC NSSKLRGLKR KWIRCSAQAT VLHLKKFIAK KLNLSSFNEL
210 220 230 240
DILCNEEILG KDHTLKFVVV TRWRFKKAPL LLHYRPKMDL L
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JDR0 | A0A0G2JDR0_MOUSE | Polycomb group ring finger 3 | Pcgf3 | 56 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_023116 | 155 – 178 | SICLE…RCSAQ → NTRPLWIFWHQFIPCLRTSF ENVT in isoform 2. 1 PublicationAdd BLAST | 24 | |
Alternative sequenceiVSP_023117 | 179 – 241 | Missing in isoform 2. 1 PublicationAdd BLAST | 63 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK033609 mRNA Translation: BAC28388.1 AK089091 mRNA Translation: BAC40745.1 BC049266 mRNA Translation: AAH49266.1 |
CCDSi | CCDS19512.1 [Q8BTQ0-1] |
RefSeqi | NP_766304.2, NM_172716.4 [Q8BTQ0-1] |
Genome annotation databases
Ensembli | ENSMUST00000046975; ENSMUSP00000041790; ENSMUSG00000033623 [Q8BTQ0-1] ENSMUST00000112597; ENSMUSP00000108216; ENSMUSG00000033623 [Q8BTQ0-2] |
GeneIDi | 69587 |
KEGGi | mmu:69587 |
UCSCi | uc008yod.1, mouse [Q8BTQ0-2] uc008yoe.1, mouse [Q8BTQ0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK033609 mRNA Translation: BAC28388.1 AK089091 mRNA Translation: BAC40745.1 BC049266 mRNA Translation: AAH49266.1 |
CCDSi | CCDS19512.1 [Q8BTQ0-1] |
RefSeqi | NP_766304.2, NM_172716.4 [Q8BTQ0-1] |
3D structure databases
AlphaFoldDBi | Q8BTQ0 |
SMRi | Q8BTQ0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 213553, 1 interactor |
IntActi | Q8BTQ0, 1 interactor |
STRINGi | 10090.ENSMUSP00000041790 |
PTM databases
PhosphoSitePlusi | Q8BTQ0 |
Proteomic databases
MaxQBi | Q8BTQ0 |
PaxDbi | Q8BTQ0 |
PRIDEi | Q8BTQ0 |
ProteomicsDBi | 287889 [Q8BTQ0-1] 287890 [Q8BTQ0-2] |
Protocols and materials databases
Antibodypediai | 4972, 160 antibodies from 29 providers |
DNASUi | 69587 |
Genome annotation databases
Ensembli | ENSMUST00000046975; ENSMUSP00000041790; ENSMUSG00000033623 [Q8BTQ0-1] ENSMUST00000112597; ENSMUSP00000108216; ENSMUSG00000033623 [Q8BTQ0-2] |
GeneIDi | 69587 |
KEGGi | mmu:69587 |
UCSCi | uc008yod.1, mouse [Q8BTQ0-2] uc008yoe.1, mouse [Q8BTQ0-1] |
Organism-specific databases
CTDi | 10336 |
MGIi | MGI:1916837, Pcgf3 |
VEuPathDBi | HostDB:ENSMUSG00000033623 |
Phylogenomic databases
eggNOGi | KOG2660, Eukaryota |
GeneTreei | ENSGT00940000158395 |
HOGENOMi | CLU_046427_4_3_1 |
InParanoidi | Q8BTQ0 |
OMAi | ILCNNEL |
OrthoDBi | 1203951at2759 |
PhylomeDBi | Q8BTQ0 |
TreeFami | TF324206 |
Miscellaneous databases
BioGRID-ORCSi | 69587, 3 hits in 73 CRISPR screens |
ChiTaRSi | Pcgf3, mouse |
PROi | PR:Q8BTQ0 |
RNActi | Q8BTQ0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000033623, Expressed in dorsal root ganglion and 271 other tissues |
Genevisiblei | Q8BTQ0, MM |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR032443, RAWUL IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF16207, RAWUL, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PCGF3_MOUSE | |
Accessioni | Q8BTQ0Primary (citable) accession number: Q8BTQ0 Secondary accession number(s): Q8BZT0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 20, 2007 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot