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Protein

RAS guanyl-releasing protein 4

Gene

Rasgrp4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri540 – 590Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
Biological processDifferentiation
LigandCalcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5673001 RAF/MAP kinase cascade

Names & Taxonomyi

Protein namesi
Recommended name:
RAS guanyl-releasing protein 4
Gene namesi
Name:Rasgrp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2386851 Rasgrp4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003152141 – 673RAS guanyl-releasing protein 4Add BLAST673

Proteomic databases

PaxDbiQ8BTM9
PRIDEiQ8BTM9

PTM databases

iPTMnetiQ8BTM9
PhosphoSitePlusiQ8BTM9

Expressioni

Tissue specificityi

Expressed by mast cells and their progenitors (at protein level).2 Publications

Developmental stagei

Expressed at all stages in the development of mast cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000030589 Expressed in 23 organ(s), highest expression level in bone marrow
CleanExiMM_RASGRP4
ExpressionAtlasiQ8BTM9 baseline and differential
GenevisibleiQ8BTM9 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi231357, 1 interactor
IntActiQ8BTM9, 2 interactors
STRINGi10090.ENSMUSP00000092200

Structurei

3D structure databases

ProteinModelPortaliQ8BTM9
SMRiQ8BTM9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 175N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST127
Domaini201 – 432Ras-GEFPROSITE-ProRule annotationAdd BLAST232
Domaini466 – 501EF-handAdd BLAST36

Domaini

The phorbol-ester/DAG-type zinc finger mediates the binding and the functional activation by DAG.

Sequence similaritiesi

Belongs to the RASGRP family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri540 – 590Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410KD3C Eukaryota
ENOG410XQ12 LUCA
GeneTreeiENSGT00880000137870
HOVERGENiHBG007513
InParanoidiQ8BTM9
KOiK12363
PhylomeDBiQ8BTM9

Family and domain databases

CDDicd00029 C1, 1 hit
cd00155 RasGEF, 1 hit
Gene3Di1.10.840.10, 1 hit
InterProiView protein in InterPro
IPR020454 DAG/PE-bd
IPR002219 PE/DAG-bd
IPR008937 Ras-like_GEF
IPR000651 Ras-like_Gua-exchang_fac_N
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
PANTHERiPTHR23113 PTHR23113, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF00617 RasGEF, 1 hit
PRINTSiPR00008 DAGPEDOMAIN
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00147 RasGEF, 1 hit
SUPFAMiSSF48366 SSF48366, 1 hit
PROSITEiView protein in PROSITE
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BTM9-1) [UniParc]FASTAAdd to basket
Also known as: RASGRP4alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNRKDIKRKS HQECSGKAGG RGRSRQARRH KTCPTPREIS KVMASMNLGV
60 70 80 90 100
LSESSCSEDE LLEECIRCFD SAGSLRRGDH ILKMVLTMHS WVLPSSELAA
110 120 130 140 150
RLLTSYQKAA KDAQELRQLQ ICYLVRYWLT HHHEAVHQEP QLEAVISRFW
160 170 180 190 200
TTVAQEGNMA QRSLGDASSL LSPGGPGPPP PMSSPGLGKK RKVSLLFDHL
210 220 230 240 250
ETEELAQHLT YLEFRSFQAI TPQDLRGYVL QGSVRGCPAL EGSVGLSNSV
260 270 280 290 300
SRWVQVMVLS RPGPAQRAQV LDKFIRVAQR LHQLQNFNTL MAVTGGLCHS
310 320 330 340 350
AISRLKDSHV HLSPDSTKAL LELTELLSSH NNYAHYRRTW AGCTGFRLPV
360 370 380 390 400
LGVHLKDLVS LYEAHPDRLP DGRLHLPKLN SLYLRLQELM ALQGQHPPCS
410 420 430 440 450
ANEDLLHLLT LSLDLFYTED EIYELSYARE PRCPKSLPPS PFKAPVVVEW
460 470 480 490 500
AQGVTPKPDS VTLGQHVEQL VESVFKNYDP EGRGSISLED FERLSGNFPF
510 520 530 540 550
ACHGLHPPPR HGSGSFSREE LTKYLLHASA ICSKLGLAFL HAFQEVTFRK
560 570 580 590 600
PTFCHSCSGF LWGVTKQGYR CRDCGLCCHR HCRDQVRVEC KKRPETKGDP
610 620 630 640 650
GPPGAPVPAT SLPPANCGSE ESLSYTLSPD PESGCHLRHA WTQTESSHSS
660 670
WEPEVVPCPA RVLPSRASSK PSV
Length:673
Mass (Da):74,907
Last modified:March 1, 2003 - v1
Checksum:i418DACEF8064AD18
GO
Isoform 2 (identifier: Q8BTM9-2) [UniParc]FASTAAdd to basket
Also known as: RASGRP4beta

The sequence of this isoform differs from the canonical sequence as follows:
     560-560: F → FVSTGP

Show »
Length:678
Mass (Da):75,349
Checksum:i3D24B36EB8FE2454
GO
Isoform 3 (identifier: Q8BTM9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     561-611: LWGVTKQGYR...PPGAPVPATS → VSTGPVSTLG...PHLRYSTEFI
     612-673: Missing.

Note: Due to an intron retention. No experimental confirmation available.
Show »
Length:611
Mass (Da):68,428
Checksum:i3693356ABF9B65E6
GO
Isoform 4 (identifier: Q8BTM9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     574-578: CGLCC → TAGIK
     579-673: Missing.

Note: Minor isoform which is not activated by DAG and probably not functional. Preferentially expressed in the C3H/HeJ strain. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:578
Mass (Da):64,654
Checksum:iEF858A9B62D5411F
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QKB7E9QKB7_MOUSE
RAS guanyl-releasing protein 4
Rasgrp4
679Annotation score:
E0CZ06E0CZ06_MOUSE
RAS guanyl-releasing protein 4
Rasgrp4
659Annotation score:
A0A0N4SVP1A0A0N4SVP1_MOUSE
RAS guanyl-releasing protein 4
Rasgrp4
484Annotation score:
A0A0N4SW33A0A0N4SW33_MOUSE
RAS guanyl-releasing protein 4
Rasgrp4
581Annotation score:
A0A0N4SVV6A0A0N4SVV6_MOUSE
RAS guanyl-releasing protein 4
Rasgrp4
576Annotation score:
A0A0N4SUN7A0A0N4SUN7_MOUSE
RAS guanyl-releasing protein 4
Rasgrp4
604Annotation score:
A0A0N4SUQ3A0A0N4SUQ3_MOUSE
RAS guanyl-releasing protein 4
Rasgrp4
639Annotation score:
E0CX54E0CX54_MOUSE
RAS guanyl-releasing protein 4
Rasgrp4
429Annotation score:
F7BK25F7BK25_MOUSE
RAS guanyl-releasing protein 4
Rasgrp4
85Annotation score:

Sequence cautioni

The sequence BAE32802 differs from that shown. Reason: Frameshift at position 672.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti182M → I in AAK81694 (PubMed:11956218).Curated1
Sequence conflicti182M → I in AAK84299 (PubMed:11956218).Curated1
Sequence conflicti443K → R in AAK81694 (PubMed:11956218).Curated1
Sequence conflicti443K → R in AAK84299 (PubMed:11956218).Curated1
Sequence conflicti468E → G in AAK81694 (PubMed:11956218).Curated1
Sequence conflicti468E → G in AAK84299 (PubMed:11956218).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030483560F → FVSTGP in isoform 2. 1 Publication1
Alternative sequenceiVSP_030484561 – 611LWGVT…VPATS → VSTGPVSTLGSHLPWIVSAP HTLTWDSVGTGFWWWWGLQK CPHLRYSTEFI in isoform 3. 1 PublicationAdd BLAST51
Alternative sequenceiVSP_030485574 – 578CGLCC → TAGIK in isoform 4. 1 Publication5
Alternative sequenceiVSP_030486579 – 673Missing in isoform 4. 1 PublicationAdd BLAST95
Alternative sequenceiVSP_030487612 – 673Missing in isoform 3. 1 PublicationAdd BLAST62

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF331457 mRNA Translation: AAK84299.1
AY040628 mRNA Translation: AAK81694.1
AY196476 mRNA Translation: AAO20841.1
AK049562 mRNA Translation: BAC33811.1
AK089282 mRNA Translation: BAC40827.1
AK154746 mRNA Translation: BAE32802.1 Frameshift.
AK162667 mRNA Translation: BAE37013.1
AK165106 mRNA Translation: BAE38037.1
CCDSiCCDS85257.1 [Q8BTM9-1]
CCDS85258.1 [Q8BTM9-2]
RefSeqiNP_001167626.1, NM_001174155.1 [Q8BTM9-2]
NP_660131.2, NM_145149.4 [Q8BTM9-1]
UniGeneiMm.465324

Genome annotation databases

EnsembliENSMUST00000032811; ENSMUSP00000032811; ENSMUSG00000030589 [Q8BTM9-1]
ENSMUST00000159351; ENSMUSP00000124183; ENSMUSG00000030589 [Q8BTM9-4]
ENSMUST00000159975; ENSMUSP00000125137; ENSMUSG00000030589 [Q8BTM9-2]
GeneIDi233046
KEGGimmu:233046
UCSCiuc009gas.2 mouse [Q8BTM9-1]
uc009gat.1 mouse [Q8BTM9-3]
uc012fha.1 mouse [Q8BTM9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF331457 mRNA Translation: AAK84299.1
AY040628 mRNA Translation: AAK81694.1
AY196476 mRNA Translation: AAO20841.1
AK049562 mRNA Translation: BAC33811.1
AK089282 mRNA Translation: BAC40827.1
AK154746 mRNA Translation: BAE32802.1 Frameshift.
AK162667 mRNA Translation: BAE37013.1
AK165106 mRNA Translation: BAE38037.1
CCDSiCCDS85257.1 [Q8BTM9-1]
CCDS85258.1 [Q8BTM9-2]
RefSeqiNP_001167626.1, NM_001174155.1 [Q8BTM9-2]
NP_660131.2, NM_145149.4 [Q8BTM9-1]
UniGeneiMm.465324

3D structure databases

ProteinModelPortaliQ8BTM9
SMRiQ8BTM9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231357, 1 interactor
IntActiQ8BTM9, 2 interactors
STRINGi10090.ENSMUSP00000092200

PTM databases

iPTMnetiQ8BTM9
PhosphoSitePlusiQ8BTM9

Proteomic databases

PaxDbiQ8BTM9
PRIDEiQ8BTM9

Protocols and materials databases

DNASUi233046
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032811; ENSMUSP00000032811; ENSMUSG00000030589 [Q8BTM9-1]
ENSMUST00000159351; ENSMUSP00000124183; ENSMUSG00000030589 [Q8BTM9-4]
ENSMUST00000159975; ENSMUSP00000125137; ENSMUSG00000030589 [Q8BTM9-2]
GeneIDi233046
KEGGimmu:233046
UCSCiuc009gas.2 mouse [Q8BTM9-1]
uc009gat.1 mouse [Q8BTM9-3]
uc012fha.1 mouse [Q8BTM9-2]

Organism-specific databases

CTDi115727
MGIiMGI:2386851 Rasgrp4

Phylogenomic databases

eggNOGiENOG410KD3C Eukaryota
ENOG410XQ12 LUCA
GeneTreeiENSGT00880000137870
HOVERGENiHBG007513
InParanoidiQ8BTM9
KOiK12363
PhylomeDBiQ8BTM9

Enzyme and pathway databases

ReactomeiR-MMU-5673001 RAF/MAP kinase cascade

Miscellaneous databases

PROiPR:Q8BTM9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030589 Expressed in 23 organ(s), highest expression level in bone marrow
CleanExiMM_RASGRP4
ExpressionAtlasiQ8BTM9 baseline and differential
GenevisibleiQ8BTM9 MM

Family and domain databases

CDDicd00029 C1, 1 hit
cd00155 RasGEF, 1 hit
Gene3Di1.10.840.10, 1 hit
InterProiView protein in InterPro
IPR020454 DAG/PE-bd
IPR002219 PE/DAG-bd
IPR008937 Ras-like_GEF
IPR000651 Ras-like_Gua-exchang_fac_N
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
PANTHERiPTHR23113 PTHR23113, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF00617 RasGEF, 1 hit
PRINTSiPR00008 DAGPEDOMAIN
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00147 RasGEF, 1 hit
SUPFAMiSSF48366 SSF48366, 1 hit
PROSITEiView protein in PROSITE
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGRP4_MOUSE
AccessioniPrimary (citable) accession number: Q8BTM9
Secondary accession number(s): Q3TNQ1
, Q3TRL5, Q3U3I4, Q811P7, Q8BKV7, Q91ZC8, Q920A2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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